Dr Dan Peet

Daniel Peet
Biochemistry and CMGD Lecturer
School of Biological Sciences
Faculty of Sciences

Dr Peet is an Associate Professor in Biochemistry at the University of Adelaide. His research interests are centred on understanding the molecular events within cells that underpin the genomic response to hypoxia. The cellular response to hypoxia is essential for many physiological processes in normal development and adult physiology, and contributes to the pathophysiology of major human diseases, including cancer, myocardial and cerebral ischaemia, pulmonary hypertension, and wound healing. The key components of this cellular response to hypoxia are a unique family of hydroxylase enzymes that act as the primary oxygen sensors, the Hypoxic Inducible transcription Factors (HIFs) that directly regulate gene expression, and specific target genes that mediate cellular changes. His research interests extend beyond the HIFs, to the identification and characterisation of novel substrates of the oxygen-sensing hydroxylases. Understanding these processes has major implications for the diagnosis, prognosis and novel therapeutic approaches to treat human disease.

A more recent focus is on cellular metabolism, specifically the unusual metabolism displayed by proliferating cells, especially cancer cells, that mimic quiescent cells in hypoxia. Of particular interest are the photoreceptor cells in the retina, which also display cancer-like metabolism. A key aim is to determine what controls this unusual metabolism, and whether it is a similar mechanism of control to cancer cells, with important implications for retinal disease.

Connect with me

Dr Dan Peet

Dr Peet is an Associate Professor in Biochemistry at the University of Adelaide. His research interests are centred on understanding the molecular events within cells that underpin the genomic response to hypoxia. The cellular response to hypoxia is essential for many physiological processes in normal development and adult physiology, and contributes to the pathophysiology of major human diseases, including cancer, myocardial and cerebral ischaemia, pulmonary hypertension, and wound healing. The key components of this cellular response to hypoxia are a unique family of hydroxylase enzymes that act as the primary oxygen sensors, the Hypoxic Inducible transcription Factors (HIFs) that directly regulate gene expression, and specific target genes that mediate cellular changes. His research interests extend beyond the HIFs, to the identification and characterisation of novel substrates of the oxygen-sensing hydroxylases. Understanding these processes has major implications for the diagnosis, prognosis and novel therapeutic approaches to treat human disease.

A more recent focus is on cellular metabolism, specifically the unusual metabolism displayed by proliferating cells, especially cancer cells, that mimic quiescent cells in hypoxia. Of particular interest are the photoreceptor cells in the retina, which also display cancer-like metabolism. A key aim is to determine what controls this unusual metabolism, and whether it is a similar mechanism of control to cancer cells, with important implications for retinal disease.

Appointments

Date Position Institution name
2003 Associate Professor University of Adelaide

Education

Date Institution name Country Title
University of Melbourne Australia PhD

Research Interests

Biochemistry & Molecular Biology, Biochemistry and Cell Biology, Cancer Cell Biology, Medical Biochemistry: Nucleic Acids, Medical Biochemistry: Proteins and Peptides

Journals

Year Citation
2017 Peet, D., Kittipassorn, T., Wood, J., Chidlow, G. & Casson, R. (2017). HIF signalling: the eyes have it. Experimental Cell Research, 356, 2, 136-140.
10.1016/j.yexcr.2017.03.030
2017 Nagarajan, Y., Rychkov, G. & Peet, D. (2017). Modulation of TRP channel activity by hydroxylation and its therapeutic potential. Pharmaceuticals, 10, 2, 35-35.
10.3390/ph10020035
2017 Chen, D., Fabrizio, J., Wilkins, S., Dave, K., Gorman, J., Gleadle, J. ... Mercer, A. (2017). Ankyrin Repeat Proteins of Orf Virus Influence the Cellular Hypoxia Response Pathway.. J Virol, 91, 1, e01430-1-e01430-16.
10.1128/JVI.01430-16
2017 Vandyke, K., Zeissig, M., Hewett, D., Martin, S., Mrozik, K., Cheong, C. ... Zannettino, A. (2017). HIF-2α promotes dissemination of plasma cells in multiple myeloma by regulating CXCL12/CXCR4 and CCR1. Cancer Research, 77, 20, 5452-5463.
10.1158/0008-5472.CAN-17-0115
2016 Casson, R., Wood, J., Han, G., Kittipassorn, T., Peet, D. & Chidlow, G. (2016). M-type pyruvate kinase isoforms and lactate dehydrogenase a in the mammalian retina: metabolic implications. Investigative Ophthalmology and Visual Science, 57, 1, 66-80.
10.1167/iovs.15-17962
2016 Sullivan, A., Peet, D. & Whitelaw, M. (2016). MAGED1 is a novel regulator of a select subset of bHLH PAS transcription factors. FEBS Journal, 283, 18, 3488-3502.
10.1111/febs.13824
2016 Scholz, C., Rodriguez, J., Pickel, C., Burr, S., Fabrizio, J., Nolan, K. ... Taylor, C. (2016). FIH regulates cellular metabolism through hydroxylation of the deubiquitinase OTUB1. C. Simon (Ed.). PLoS Biology, 14, 1, e1002347-1-e1002347-22.
10.1371/journal.pbio.1002347
2015 Ng, S., Wood, J., Chidlow, G., Han, G., Kittipassorn, T., Peet, D. & Casson, R. (2015). Cancer-like metabolism of the mammalian retina. Clinical and Experimental Ophthalmology, 43, 4, 367-376.
10.1111/ceo.12462
2015 Kind, K., Tam, K., Banwell, K., Gauld, A., Russell, D., Macpherson, A. ... Thompson, J. (2015). Oxygen-regulated gene expression in murine cumulus cells. Reproduction, Fertility and Development, 27, 2, 407-418.
10.1071/RD13249
2015 Ng, S., Wood, J., Chidlow, G., Peet, D. & Casson, R. (2015). Potential adverse effects to the retina of cancer therapy targeting pyruvate kinase M2. Acta Oncologica, 54, 1, 136-137.
10.3109/0284186X.2014.922217
2015 Kittipassorn, T. & Peet, D. (2015). Hypoxia inducible factor single nucleotide polymorphisms: exploring the role of HIF polymorphisms in retinal disease. Clinical and Experimental Ophthalmology, 43, 1, 1-2.
10.1111/ceo.12493
2015 Karttunen, S., Duffield, M., Scrimgeour, N., Squires, L., Lim, W., Dallas, M. ... Peet, D. (2015). Oxygen-dependent hydroxylation by FIH regulates the TRPV3 ion channel. Journal of Cell Science, 128, 2, 225-231.
10.1242/jcs.158451
2014 Bersten, D., Bruning, J., Peet, D. & Whitelaw, M. (2014). Human variants in the Neuronal Basic Helix-Loop-Helix/ Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. M. Katoh (Ed.). PLoS One, 9, 1, e85768-1-e85768-11.
10.1371/journal.pone.0085768
2014 Sullivan, A., Raimondo, A., Schwab, T., Bruning, J., Froguel, P., Farooqi, I. ... Whitelaw, M. (2014). Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2. Biochemical Journal, 461, 3, 403-412.
10.1042/BJ20131618
2013 Ramachandrappa, S., Raimondo, A., Cali, A., Keogh, J., Henning, E., Saeed, S. ... Farooqi, I. (2013). Rare variants in single-minded 1 (SIM1) are associated with severe obesity. Journal of Clinical Investigation, 123, 7, 3042-3050.
10.1172/JCI68016
2013 Bersten, D., Sullivan, A., Peet, D. & Whitelaw, M. (2013). BHLH-PAS proteins in cancer. Nature Reviews. Cancer, 13, 12, 827-841.
10.1038/nrc3621
2013 Ramachandrappa, S., Raimondo, A., Cali, A., Keogh, J., Henning, E., Saeed, S. ... Farooqi, I. (2013). Erratum: Rare variants in single-minded 1 (SIM1) are associated with severe obesity (Journal of Clinical Investigation (2013) 123:7 (3042-3050) DOI: 10.1172/JCI68016). Journal of Clinical Investigation, 123, 8, 3635-.
10.1172/JCI72103
2013 Bonnefond, A., Raimondo, A., Stutzmann, F., Ghoussaini, M., Ramachandrappa, S., Bersten, D. ... Froguel, P. (2013). Loss-of-function mutations in SIM1 contribute to obesity and Prader-Willi-like features. Journal of Clinical Investigation, 123, 7, 3037-3041.
10.1172/JCI68035
2013 Hao, N., Bhakti, V., Peet, D. & Whitelaw, M. (2013). Reciprocal regulation of the basic helix-loop-helix/Per-Arnt-Sim partner proteins, Arnt and Arnt2, during neuronal differentiation. Nucleic Acids Research, 41, 11, 5626-5638.
10.1093/nar/gkt206
2012 Wilkins, S., Karttunen, S., Hampton-Smith, R., Murchland, I., Chapman-Smith, A. & Peet, D. (2012). Factor Inhibiting HIF (FIH) recognizes distinct molecular features within hypoxia-inducible factor-α (HIF-α) versus ankyrin repeat substrates. Journal of Biological Chemistry, 287, 12, 8769-8781.
10.1074/jbc.M111.294678
2012 Olechnowicz, S., Fedele, A. & Peet, D. (2012). Hypoxic induction of the regulator of G-protein signalling 4 gene is mediated by the hypoxia-inducible factor pathway. PLoS One, 7, 9, 1-12.
10.1371/journal.pone.0044564
2012 Yusuf, D., Olechnowicz, S. & Peet, D. (2012). The Transcription factor encyclopedia. Genome Biology, 13, 3, R24:1-R24:25.
10.1186/gb-2012-13-3-r24
2011 Martin, S., Diamond, P., Gronthos, S., Peet, D. & Zannettino, A. (2011). The emerging role of hypoxia, HIF-1 and HIF-2 in multiple myeloma. Leukemia, 25, 10, 1533-1542.
10.1038/leu.2011.122
2010 Devries, I., Hampton-Smith, R., Mulvihill, M., Alverdi, V., Peet, D. & Komives, E. (2010). Consequences of IkappaB alpha hydroxylation by the factor inhibiting HIF (FIH). FEBS Letters, 584, 23, 4725-4730.
10.1016/j.febslet.2010.10.060
2010 Zhang, N., Fu, Z., Karttunen, S., Chicher, J., Gorman, J., Visk, D. ... Johnson, R. (2010). The asparaginyl hydroxylase factor inhibiting HIF-1α is an essential regulator of metabolism. Cell Metabolism, 11, 5, 364-378.
10.1016/j.cmet.2010.03.001
2010 Martin, S., Diamond, P., Williams, S., To, L., Peet, D., Fujii, N. ... Zannettino, A. (2010). Hypoxia-inducible factor-2 is a novel regulator of aberrant CXCL12 expression in multiple myeloma plasma cells. Haematologica, 95, 5, 776-784.
10.3324/haematol.2009.015628
2010 Tam, K., Russell, D., Peet, D., Bracken, C., Rodgers, R., Thompson, J. & Kind, K. (2010). Hormonally regulated follicle differentiation and luteinization in the mouse is associated with hypoxia inducible factor activity. Molecular and Cellular Endocrinology, 327, 1-2, 47-55.
10.1016/j.mce.2010.06.008
2009 Wilkins, S., Hyvarinen, J., Chicher, J., Gorman, J., Peet, D., Bilton, R. & Koivunen, P. (2009). Differences in hydroxylation and binding of Notch and HIF-1 alpha demonstrate substrate selectivity for factor inhibiting HIF-1 (FIH-1). International Journal of Biochemistry & Cell Biology, 41, 7, 1563-1571.
10.1016/j.biocel.2009.01.005
2009 Hampton-Smith, R. & Peet, D. (2009). From polyps to people: A HIghly Familiar response to hypoxia. Annals of the New York Academy of Sciences, 1177, 1, 19-29.
10.1111/j.1749-6632.2009.05035.x
2008 Lisy, K. & Peet, D. (2008). Erratum: Turn me on: Regulating HIF transcriptional activity (Cell Death and Differentiation (2008) 10.1038/sj.cdd.4402315). Cell Death and Differentiation, 15, 4, 805-.
10.1038/sj.cdd.4402308
2008 Lisy, K. & Peet, D. (2008). Turn me on: regulating HIF transcriptional activity. Cell Death and Differentiation, 15, 4, 642-649.
10.1038/sj.cdd.4402315
2008 Zheng, X., Karttunen, S., Dias, J., Zheng, X., Gradin, K., Wallis, T. ... Poellinger, L. (2008). Interaction with factor inhibiting HIF-1 defines an additional mode of cross-coupling between the Notch and hypoxia signaling pathways. Proceedings of the National Academy of Sciences of the United States of America, 105, 9, 3368-3373.
10.1073/pnas.0711591105
2007 Karttunen, S., Hampton-Smith, R. & Peet, D. (2007). Characterization of ankyrin repeat-containing proteins as substrates of the asparaginyl hydroxylase factor inhibiting hypoxia-inducible transcription factor. Methods in Enzymology, 435, 61-85.
10.1016/S0076-6879(07)35004-0
2007 Gorman, J., Wallis, T., Dave, K., Hamilton, B., Headlam, M., Linke, S. & Peet, D. (2007). Quantitative approaches for analysis of regulatory post-translational modifications. MOLECULAR & CELLULAR PROTEOMICS, 6, 8, 15-15.
2006 Bracken, C., Fedele, A., Karttunen, S., Balrak, W., Lisy, K., Whitelaw, M. & Peet, D. (2006). Cell-specific regulation of hypoxia-inducible factor (HIF)-1α and HIF-2α stabilization and transactivation in a graded oxygen environment. Journal of Biological Chemistry, 281, 32, 22575-22585.
10.1074/jbc.M600288200
2005 Bracken, C., Whitelaw, M. & Peet, D. (2005). Activity of hypoxia-inducible factor 2α is regulated by association with the NF-κB essential modulator. Journal of Biological Chemistry, 280, 14, 14240-14251.
10.1074/jbc.M409987200
2004 Karttunen, S., Stojkoski, C., Kewley, R., Booker, G., Whitelaw, M. & Peet, D. (2004). Substrate requirements of the oxygen-sensing asparaginyl hydroxylase factor-inhibiting hypoxia-inducible factor. Biological Chemistry, 279, 14, 14391-14397.
10.1074/jbc.M313614200
2004 Peet, D., Lando, D., Whelan, D., Whitelaw, M. & Gorman, J. (2004). Oxygen-dependent asparagine hydroxylation. Methods in Enzymology, 381, 467-487.
10.1016/S0076-6879(04)81031-0
2003 Lees, M., Peet, D. & Whitelaw, M. (2003). Defining the role for XAP2 in stabilization of the dioxin receptor. Journal of Biological Chemistry, 278, 38, 35878-35888.
10.1074/jbc.M302430200
2003 Lando, D., Gorman, J., Whitelaw, M. & Peet, D. (2003). Oxygen-dependent regulation of hypoxia-inducible factors by prolyl and asparaginyl hydroxylation. FEBS Journal, 270, 5, 781-790.
10.1046/j.1432-1033.2003.03445.x
2003 Bracken, C., Whitelaw, M. & Peet, D. (2003). The hypoxia-inducible factors: key transcriptional regulators of hypoxic responses. Cellular and Molecular Life Sciences, 60, 7, 1376-1393.
10.1007/s00018-003-2370-y
2002 Lando, D., Peet, D., Gorman, J., Whelan, D., Whitelaw, M. & Bruick, R. (2002). FIH-1 is an asparaginyl hydroxylase enzyme that regulates the transcriptional activity of hypoxia-inducible factor. Genes & Development, 16, 12, 1466-1471.
10.1101/gad.991402
2002 Fedele, A., Whitelaw, M. & Peet, D. (2002). Regulation of gene expression by the hypoxia-inducible factors. Molecular Interventions, 2, 4, 229-243.
10.1124/mi.2.4.229
2002 Lando, D., Peet, D., Whelan, D., Gorman, J. & Whitelaw, M. (2002). Asparagine hydroxylation of the HIF transactivation domain: A hypoxic switch. Science, 295, 5556, 858-861.
10.1126/science.1068592
2002 Lando, D., Peet, D., Pongratz, I. & Whitelaw, M. (2002). Mammalian two-hybrid assay showing redox control of HIF-like factor. Methods in Enzymology, 353, 3-10.
10.1016/S0076-6879(02)53031-7
1998 Doyle, D., Peet, D., Mangelsdorf, D. & Corey, D. (1998). Changing the ligand-binding specificity of the nuclear hormone receptor RXR.. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 215, U876-U876.
1998 Corey, D., Doyle, D., Peet, D. & Mangelsdorf, D. (1998). Engineering novel specificities for ligand dependent transcriptional activation. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 216, U256-U256.
1998 Peet, D., Turley, S., Ma, W., Janowski, B., Lobaccaro, J., Hammer, R. & Mangelsdorf, D. (1998). Cholesterol and bile acid metabolism are impaired in mice lacking the nuclear oxysterol receptor LXRα. Cell, 93, 5, 693-704.
10.1016/S0092-8674(00)81432-4
1998 Peet, D., Doyle, D., Corey, D. & Mangelsdorf, D. (1998). Engineering novel specificities for ligand-activated transcription in the nuclear hormone receptor RXR. Chemistry and Biology, 5, 1, 13-21.
10.1016/S1074-5521(98)90083-7
1998 Peet, D., Janowski, B. & Mangelsdorf, D. (1998). The LXRs: A new class of oxysterol receptors. Current Opinion in Genetics and Development, 8, 5, 571-575.
10.1016/S0959-437X(98)80013-0
1996 Negri, A., Peet, D., Rivett, D., Rivett, R. & Cornell, H. (1996). Covalently Bound Fatty Acids and Ceramides in Wool. Journal of the Textile Institute, 87, 3, 608-611.
10.1080/00405009608631365
1996 Jones, L., Peet, D., Danks, D., Negri, A. & Rivett, D. (1996). Hairs from patients with maple syrup urine disease show a structural defect in the fiber cuticle. Journal of Investigative Dermatology, 106, 3, 461-464.
10.1111/1523-1747.ep12343618
1995 Peet, D., Rivett, D. & Wettenhall, R. (1995). The Chemistry of the Cuticle Surface of Keratin Fibers. Textile Research Journal, 65, 1, 58-59.
10.1177/004051759506500109
1992 Peet, D., Wettenhall, R., Rivett, D. & Allen, A. (1992). A comparative study of covalently-bound fatty acids in keratinized tissues. Comparative Biochemistry and Physiology -- Part B: Biochemistry and, 102, 2, 363-366.
10.1016/0305-0491(92)90135-E

Book Chapters

Year Citation
2007 Hampton-Smith, R. & Peet, D. (2007). Hypoxia Inducible Factor. In T. M. H. E. Staff (Ed.), McGraw-Hill Yearbook of Science & Technology 2007 (pp. 108-111). United States: McGraw-Hill.
2006 Peet, D. & Karttunen, S. (2006). Regulation of HIF: asparaginyl hydroxylation. In D. Chadwick & J. Goode (Eds.), Signalling pathways in acute oxygen sensing (pp. 37-51). The Atrium, Southern Gate, Chichester PO19 8SQ, UK: Wiley.
10.1002/9780470035009.ch5

Conference Papers

Year Citation
2006 Duchen, M., Semenza, G., Ratcliffe, P., López-Barneo, J., Prabhakar, N., Acker, H. ... Archer, S. (2006). Discussion.
2006 Murphy, M., Schumacker, P., Kummer, W., Archer, S., Harris, A., Sylvester, J. ... Peet, D. (2006). Discussion.
2006 Ratcliffe, P., Peet, D., Chandel, N., Semenza, G., Stolze, I., Pouysségur, J. ... Schumacker, P. (2006). Discussion.
2005 Peet, D. (2005). Cellular response to hypoxia: A key role for oxygen-sensing peptidyl hydroxylases. 29th Annual Scientific Meeting of the Australian Section of the International-Society-Heart-Research. Perth, AUSTRALIA.
1999 Rivett, D. & Peet, D. (1999). Fatty acids chemically bound to protein forms the hydrophic membrane of the hair cuticle. Denmark.

Conference Items

Year Citation
2016 Scholz, C., Rodriguez, J., Pickel, C., Burr, S., Fabrizio, J. -. A., Nolan, K. ... Taylor, C. (2016). FIH regulates cellular metabolism through hydroxylation of the deubiquitinase OTUB1.
2009 Lisy, K. & Peet, D. J. (2009). Is FIH-1 Phosphorylated? 2-DE Analysis of FIH-1 Isoforms. Hypoxia and Consequences: From Molecule to Malady. New York, New York.
2007 Lisy, K. & Peet, D. J. (2007). Regulation of Gene Expression via the Asparaginyl Hydroxylase Factor Inhibiting HIF (FIH-1). Lorne Cancer Conference. Lorne, Australia.
2006 Lisy, K. & Peet, D. J. (2006). Characterising Expression of the Asparaginyl Hydroxylase Factor Inhibiting HIF (FIH-1). Lorne Genome Conference. Lorne, Australia.
2005 Lisy, K. & Peet, D. J. (2005). Characterisation of FIH-1 Expression. Australian Society for Biochemistry and Molecular Biology ComBio Conference. Adelaide, Australia.

Patents

Year Citation
2009 Whitelaw, M., Peet, D., Karttunen, S., Lando, D. & Gorman, J.; (2009); Asparagine Hydroxylation of the CAD Domain of a HIF Protein;
2008 Whitelaw, M., Peet, D., Karttunen, S., Gorman, J. & Lando, D.; (2008); Asparagine Hydroxylation of the CAD Domain of a HIF Protein;
2006 Gustafsson, M., Lendahl, U., Bondesson Bolin, M., Poellinger, L., Pereira, T., Ruas, J. ... Peet, D.; (2006); Modulation of cellular hypoxia responses through Notch signalling;
2002 Whitelaw, M., Lando, D., Peet, D., Gorman, J. & Karttunen, S.; (2002); Asparagine hydroxylation of the CAD domain of a HIF protein;

Memberships

Date Role Membership Country
2009 - 2011 Member New York Academy of Sciences
2004 - ongoing Member Federation of American Societies for Experimental Biology United States
2004 - ongoing Member American Society of Biochemistry and Molecular Biology United States
1990 - ongoing Member Australian Society of Biochemistry and Molecular Biology Australia

Editorial Boards

Date Role Editorial Board Name Country
2015 - ongoing Consulting Editor Journal of Hypoxia Australia
Position
Biochemistry and CMGD Lecturer
Phone
83135367
Fax
8313 4362
Campus
North Terrace
Building
Molecular Life Sciences
Room Number
3 40
Org Unit
Molecular and Cellular Biology

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