Dr David Sharkey

NHMRC Postdoctoral Researcher (B)

School of Biomedicine

Faculty of Health and Medical Sciences

Eligible to supervise Masters and PhD (as Co-Supervisor) - email supervisor to discuss availability.

Dr David Sharkey
PhD (Medicine), Discipline of Obstetrics and Gynaecology, The University of Adelaide.

Thesis entitled: Seminal plasma regulation of the post-coital inflammatory response in the human cervix'
Supervisors; Professor Sarah Robertson and Professor Robert Norman

My Research
Career
Publications
Grants and Funding
Teaching
Supervision
Professional Activities
Contact

Dr David Sharkey is a postdoctoral research associate within the Reproductive Immunology laboratory at The University of Adelaide’s, Robinson Research Institute. Since completing his PhD at The University of Adelaide in 2005, under the supervision of Professors Sarah Robertson and Robert Norman, he has continued his postdoctoral training within the Robertson laboratory where he has now amassed almost two decades of experience in investigating how male seminal fluid interacts with the female immune response to prepare the female reproductive tract for pregnancy. He is first author on ground-breaking research papers demonstrating that after coitus, contact with seminal fluid elicits an immune response in the cervix of women. He is also a chief investigator on a recently awarded NHMRC project grant which seeks to determine the extent and significance of the link between factors present in male semen and the establishment of immune tolerance in women necessary for successful pregnancy.

Appointments

Date Position Institution name

2005 - ongoing Senior Postdoctoral Research Associate University of Adelaide

Date	Position	Institution name
2005 - ongoing	Senior Postdoctoral Research Associate	University of Adelaide

Awards and Achievements

Date	Type	Title	Institution Name	Amount
2015	Achievement	High Performance Researcher Program
2011	Award	Travel award, Society for Reproduction and Fertility
2011	Award	Travel Award, Research Centre for Reproductive Health	University of Adelaide	$500
2010	Award	Travel Award, Research Centre for Reproductive Health	University of Adelaide
2010	Achievement	Robinson Research Institute Mentor Program Training
2009	Achievement	Researcher Education and Development, Future Research Leaders Course	University of Adelaide
2009	Award	Travel Award, Research Centre for Reproductive Health	University of Adelaide	$295
2008	Achievement	Australian Society for Medical Research Professional Development Workshop
2008	Achievement	Higher Degree Research Supervisor Induction Course	University of Adelaide
2008	Award	Travel Award, Research Centre for Reproductive Health	University of Adelaide	$1000
2008	Award	Travel Award, ARC / NHMRC Research Network in Genes and Environment in Development (NGED)		$1000
2008	Award	Overseas Conference Travel Award, Faculty of Health Sciences	University of Adelaide	$1000
2007	Award	Travel Award, Research Centre for Reproductive Health		$1000
2007	Award	Travel Award, ARC / NHMRC Research Network in Genes and Environment in Development (NGED)		$1000
2007	Award	Overseas Conference Travel Award, Faculty of Health Sciences	University of Adelaide	$1500
2006	Award	Travel Award, Society for Reproduction and Fertility (SRF)		£500
2004	Scholarship	Postgraduate Travel Award, Faculty of Health Sciences Travel Scholarship		$1500
2003	Award	Holden Young investigator Award
2002	Award	Junior Scientist Award
2000	Award	Student Poster Award		$250
2000	Award	Travel Award, Australian Society for Medical Research		$150

Education

Date	Institution name	Country	Title
2000 - 2005	University of Adelaide	Australia	PhD
1999	University of Adelaide	Australia	Bachelor of Science (Honours) in Obstetrics and Gynaecology – First Class

Journals

Year	Citation
2022	Castleton, P. E., Deluao, J. C., Sharkey, D. J., & McPherson, N. O. (2022). Measuring Reactive Oxygen Species in Semen for Male Preconception Care: A Scientist Perspective. Antioxidants, 11(2), 23 pages. DOI
2022	Moldenhauer, L. M., Jin, M., Wilson, J. J., Green, E. S., Sharkey, D. J., Salkeld, M. D., . . . Robertson, S. A. (2022). Regulatory T cell proportion and phenotype are altered in women using oral contraception.. Endocrinology, bqac098. DOI
2021	Foyle, K. L., Sharkey, D. J., Moldenhauer, L. M., Green, E. S., Wilson, J. J., Roccisano, C. J., . . . Robertson, S. A. (2021). Effect of Intralipid infusion on peripheral blood T cells and plasma cytokines in women undergoing assisted reproduction treatment. Clinical and Translational Immunology, 10(8), e1328-1-e1328-17. DOI Scopus2
2021	Tamessar, C. T., Trigg, N. A., Nixon, B., Skerrett-Byrne, D. A., Sharkey, D. J., Robertson, S. A., . . . Schjenken, J. E. (2021). Roles of male reproductive tract extracellular vesicles in reproduction. American Journal of Reproductive Immunology, 85(2), e13338-1-e13338-12. DOI Scopus13 WoS9 Europe PMC2
2021	Schjenken, J. E., Sharkey, D. J., Green, E. S., Chan, H. Y., Matias, R. A., Moldenhauer, L. M., & Robertson, S. A. (2021). Sperm modulate uterine immune parameters relevant to embryo implantation and reproductive success in mice. Communications Biology, 4(1), 1-14. DOI Scopus11 WoS11 Europe PMC4
2021	Schjenken, J. E., Moldenhauer, L. M., Sharkey, D. J., Chan, H. Y., Chin, P. Y., Fullston, T., . . . Robertson, S. A. (2021). High fat diet alters male seminal plasma composition to impair female immune adaptation for pregnancy in mice. Endocrinology, 162(10), 1-20. DOI Scopus4 WoS4 Europe PMC2
2020	Wahid, H. H., Chin, P. Y., Sharkey, D. J., Diener, K. R., Hutchinson, M. R., Rice, K. C., . . . Robertson, S. A. (2020). Toll-like receptor-4 antagonist (+)-naltrexone protects against carbamyl-platelet activating factor (Cpaf)-induced preterm labor in mice. American Journal of Pathology, 190(5), 1030-1045. DOI Scopus9 WoS9 Europe PMC5
2020	Sorvina, A., Shandala, T., Wang, S., Sharkey, D. J., Parkinson-Lawrence, E., Selemidis, S., & Brooks, D. A. (2020). CDKI-73 is a novel pharmacological inhibitor of Rab11 cargo delivery and innate immune secretion. Cells, 9(2), 1-17. DOI Scopus4 WoS4 Europe PMC4
2019	Chin, P. Y., Dorian, C., Sharkey, D. J., Hutchinson, M. R., Rice, K. C., Moldenhauer, L. M., & Robertson, S. A. (2019). Toll-like receptor-4 antagonist (+)-naloxone confers sexually dimorphic protection from inflammation-induced fetal programming in mice. Endocrinology, 160(11), 2646-2662. DOI Scopus7 WoS8 Europe PMC7
2019	Kedzior, S., Bianco-Miotto, T., Breen, J., Diener, K. R., Donnelley, M. W., Dunning, K. R., . . . Rumbold, A. R. (2019). It takes a community to conceive: an analysis of the scope, nature and accuracy of online sources of health information for couples trying to conceive. Reproductive Biomedicine and Society Online, 9, 48-63. DOI Scopus6 Europe PMC5
2019	Nisenblat, V., Sharkey, D., Wang, Z., Evans, S., Healey, M., Maria Ohlsson Teague, E., . . . Louise Hull, M. (2019). Plasma miRNAs display limited potential as diagnostic tools for endometriosis. Journal of Clinical Endocrinology and Metabolism, 104(6), 1999-2022. DOI Scopus15 WoS17 Europe PMC12
2018	Sharkey, D., Glynn, D., Schjenken, J., Tremellen, K., & Robertson, S. (2018). Interferon-gamma inhibits seminal plasma induction of colony stimulating factor 2 in mouse and human reproductive tract epithelial cells. Biology of Reproduction, 99(3), 514-526. DOI Scopus14 WoS12 Europe PMC10
2018	Schumacher, A., Sharkey, D., Robertson, S., & Zenclussen, A. (2018). Immune cells at the fetomaternal interface: how the microenvironment modulates immune cells to foster fetal development. Journal of Immunology, 201(2), 325-334. DOI Scopus60 WoS58 Europe PMC28
2017	Sharkey, D., Tremellen, K., Briggs, N., Dekker, G., & Robertson, S. (2017). Seminal plasma pro-inflammatory cytokines interferon-3 (IFNG) and C-X-C motif chemokine ligand 8 (CXCL8) fluctuate over time within men. Human Reproduction, 32(7), 1373-1381. DOI Scopus19 WoS17 Europe PMC9
2017	Glynn, D., Heng, K., Russell, D., Sharkey, D., Robertson, S., Anand-Ivell, R., & Ivell, R. (2017). Male seminal relaxin contributes to induction of the post-mating cytokine response in the female mouse uterus. Frontiers in Physiology, 8(JUN), 422-1-422-12. DOI Scopus7 WoS6 Europe PMC3
2017	Nadeau-Vallée, M., Chin, P., Belarbi, L., Brien, M., Pundir, S., Berryer, M., . . . Chemtob, S. (2017). Antenatal suppression of il-1 protects against inflammation-induced fetal injury and improves neonatal and developmental outcomes in mice. Journal of Immunology, 198(5), 2047-2062. DOI Scopus72 WoS71 Europe PMC42
2017	Robertson, S., Zhang, B., Chan, H., Sharkey, D., Barry, S., Fullston, T., & Schjenken, J. (2017). MicroRNA regulation of immune events at conception. Molecular Reproduction and Development, 84(9), 914-925. DOI Scopus15 WoS14 Europe PMC12
2016	Schjenken, J. E., Zhang, B., Chan, H. Y., Sharkey, D. J., Fullston, T., & Robertson, S. A. (2016). miRNA Regulation of Immune Tolerance in Early Pregnancy. American Journal of Reproductive Immunology, 75(3), 272-280. DOI Scopus31 WoS31 Europe PMC21
2016	Robertson, S., & Sharkey, D. (2016). Seminal fluid and fertility in women. Fertility and Sterility, 106(3), 511-519. DOI Scopus120 WoS111 Europe PMC58
2016	Sharkey, D., Tremellen, K., Briggs, N., Dekker, G., & Robertson, S. (2016). Seminal plasma transforming growth factor-β, activin A and follistatin fluctuate within men over time. Human Reproduction, 31(10), 2183-2191. DOI Scopus22 WoS19 Europe PMC9
2015	McGuane, J., Watson, K., Zhang, J., Johan, M., Wang, Z., Kuo, G., . . . Hull, M. (2015). Seminal plasma promotes lesion development in a xenograft model of endometriosis. American Journal of Pathology, 185(5), 1409-1422. DOI Scopus9 WoS8 Europe PMC3
2015	Schjenken, J., Glynn, D., Sharkey, D., & Robertson, S. (2015). TLR4 signaling is a major mediator of the female tract response to seminal fluid in mice. Biology of Reproduction, 93(3), 68-1-68-13. DOI Scopus53 WoS41 Europe PMC28
2015	Sharkey, D., Schjenken, J., Mottershead, D., & Robertson, S. (2015). Seminal fluid factors regulate activin A and follistatin synthesis in female cervical epithelial cells. Molecular and Cellular Endocrinology, 417(Dec), 178-190. DOI Scopus14 WoS13 Europe PMC9
2014	Dasari, P., Sharkey, D., Noordin, E., Glynn, D., Hodson, L., Chin, P., . . . Ingman, W. (2014). Hormonal regulation of the cytokine microenvironment in the mammary gland. Journal of Reproductive Immunology, 106, 58-66. DOI Scopus12 WoS12 Europe PMC10
2013	Robertson, S., Prins, J., Sharkey, D., & Moldenhauer, L. (2013). Seminal fluid and the generation of regulatory T cells for embryo implantation. American Journal of Reproductive Immunology (Print), 69(4), 315-330. DOI Scopus125 WoS111 Europe PMC79
2012	Dunning, K., Watson, L., Sharkey, D., Brown, H., Norman, R., Thompson, J., . . . Russell, D. (2012). Molecular filtration properties of the mouse expanded cumulus matrix: controlled supply of metabolites and extracellular signals to cumulus cells and the oocyte. Biology of Reproduction, 87(4), 1-10. DOI Scopus19 WoS20 Europe PMC12
2012	Sharkey, D., Macpherson, A., Tremellen, K., Mottershead, D., Gilchrist, R., & Robertson, S. (2012). TGF-β mediates proinflammatory seminal fluid signaling in human cervical epithelial cells. Journal of Immunology, 189(2), 1024-1035. DOI Scopus124 WoS117 Europe PMC84
2012	Sharkey, D., Tremellen, K., Jasper, M., Gemzell-Danielsson, K., & Robertson, S. (2012). Seminal fluid induces leukocyte recruitment and cytokine and chemokine mRNA expression in the human cervix after coitus. Journal of Immunology, 188(5), 2445-2454. DOI Scopus253 WoS243 Europe PMC174
2007	Sharkey, D., Macpherson, A., Tremellen, K., & Robertson, S. (2007). Seminal plasma differentially regulates inflammatory cytokine gene expression in human cervical and vaginal epithelial cells. Molecular Human Reproduction, 13(7-8), 491-501. DOI Scopus203 WoS197 Europe PMC164
2004	Sharkey, D. J., Jasper, M. J., Tremellen, K. P., Gemzell-Danielsson, K., & Robertson, S. A. (2004). Pro-inflammatory cytokine mRNA expression is induced within the human cervix following insemination. BIOLOGY OF REPRODUCTION, 221. WoS3
2004	Glynn, D., Sharkey, D., & Robertson, S. (2004). Interferon-gamma inhibits female reproductive tract responsiveness to seminal plasma.. BIOLOGY OF REPRODUCTION, 242. WoS6
2002	Robertson, S., Ingman, W., O'Leary, S., Sharkey, D., & Tremellen, K. (2002). Transforming growth factor β — a mediator of immune deviation in seminal plasma. Journal of Reproductive Immunology, 57(1-2), 109-128. DOI Scopus230 WoS207 Europe PMC148
2001	Robertson, S., & Sharkey, D. (2001). The role of semen in induction of maternal immune tolerance to pregnancy. Seminars in Immunology, 13(4), 243-254. DOI Scopus137 WoS129 Europe PMC84
1991	Sharkey, D. J., & Kornfeld, R. (1991). Developmental regulation of processing α-mannosidases and ''intersecting'' N-acetylglucosaminyltransferase in Dictyostelium discoideum. Journal of Biological Chemistry, 266(28), 18477-18484. DOI Scopus12 Europe PMC9
1991	Sharkey, D. J., & Kornfeld, R. (1991). Developmental regulation of asparagine-linked oligosaccharide synthesis in Dictyostelium discoideum. Journal of Biological Chemistry, 266(28), 18485-18497. DOI Scopus19 Europe PMC7
1989	Sharkey, D. J., & Kornfeld, R. (1989). Identification of an N-acetylglucosaminyltransferase in Dictyostelium discoideum that transfers an "intersecting" N-acetylglucosamine residue to high mannose oligosaccharides.. The Journal of biological chemistry, 264(18), 10411-10419. DOI Scopus20 Europe PMC11
1981	Gay, C. V., Shanabrook, V. M., & Sharkey, D. J. (1981). Development of a procedure for autoradiographic localization of carbonic anhydrase at the electron microscope level. Journal of Histochemistry and Cytochemistry, 29(3), 415-419. DOI Scopus4 Europe PMC1
1981	Gay, C. V., Schraer, H., Sharkey, D. J., & Rieder, E. (1981). Carbonic anhydrase in developing avian heart, blood and chorioallantoic membrane. Comparative Biochemistry and Physiology -- Part A: Physiology, 70(2), 173-177. DOI Scopus11
1979	Gay, C. V., Sharkey, D. J., Shanabrook, V. M., & Schraer, H. (1979). Parathyroid hormone induced increase in skeletal carbonic anhydrase. Federation Proceedings, 38(3 II).

Book Chapters

Year	Citation
2019	Sharkey, D. J., & Robertson, S. A. (2019). Sex and immune receptivity for embryo transfer. In G. Kovacs, & L. Salamonsen (Eds.), How to prepare the endometrium to maximize implantation rates and IVF success (pp. 151-158). Cambridge, UK: Cambridge University Press. DOI
2018	Schjenken, J., Sharkey, D., & Robertson, S. (2018). Seminal vesicle-Secretion. In M. K. Skinner (Ed.), Encyclopedia of Reproduction (pp. 349-354). Academic Press. DOI Scopus3
2015	Robertson, S., Sharkey, D., Schjenken, J., Care, A., & Moldenhauer, L. (2015). Pathways for activating implantation tolerance in early pregnancy.. In G. CHAOUAT (Ed.), Immunology of Pregnancy (Vol. 2, pp. 1835-1874). Bentham Science Publishers. DOI Scopus25
2006	Robertson, S., Bromfield, J., Glynn, D., Sharkey, D., & Jasper, M. (2006). Actions of seminal plasma cytokines in priming female reproductive tract receptivity for embryo implantation. In Gil Mor (Ed.), Immunology of Pregnancy (pp. 148-158). New York: Springer. DOI

Conference Papers

Year	Citation
2008	Sharkey, D., & Robertson, S. (2008). Follistatin mRNA expression is regulated by seminal fluid TGF beta in human Ect1 cervical epithelial cells. In BIOLOGY OF REPRODUCTION Vol. 78 (pp. 97-98). Kona, HI: SOC STUDY REPRODUCTION. DOI WoS1
2008	Sharkey, D., & Robertson, S. (2008). Identification of TGF beta as a major active factor mediating seminal fluid signalling in human cervical epithelial cells. In BIOLOGY OF REPRODUCTION Vol. 78 (pp. 284). Kailua Kona, HI: SOC STUDY REPRODUCTION. DOI WoS1

Conference Items

Year	Citation
2018	Robertson, S. A., Sharkey, D. J., Tremellen, K. P., & Dekker, G. A. (2018). Interferon-G in seminal fluid - An 'anti-tolerance' signal in women?. Poster session presented at the meeting of JOURNAL OF REPRODUCTIVE IMMUNOLOGY. Aalborg, DENMARK: ELSEVIER IRELAND LTD. DOI
2017	Nadeau-Vallee, M., Chin, P. -Y., Belarbi, L., Brien, M. -E., Pundir, S., Beaudry-Richard, A., . . . Chemtob, S. (2017). Antenatal Suppression of Interleukin-1 Protects Against Inflammation-Induced Fetal Injury and Improves Neonatal and Developmental Outcomes in Mice.. Poster session presented at the meeting of REPRODUCTIVE SCIENCES. Orlando, FL: SAGE PUBLICATIONS INC.
2017	Nadeau-Vallee, M., Chin, P. -Y., Belarbi, L., Brien, M. -E., Pundir, S., Berryer, M. H., . . . Chemtob, S. (2017). PROTECTIVE EFFECT OF A NOVEL NONCOMPETITIVE IL-1 RECEPTOR-BIASED LIGAND IN AN INFECTION-AND INFLAMMATION-INDUCED PRETERM BIRTH AS WELL AS IN FETAL INJURY AND DEVELOPMENTAL OUTCOMES. Poster session presented at the meeting of ACTA PAEDIATRICA. WILEY.
2010	Sharkey, D. J., & Robertson, S. A. (2010). TGF beta superfamily members BMP-15, GDF-9 and Activin A act as seminal fluid signalling agents in human cervical epithelial cells. Poster session presented at the meeting of JOURNAL OF REPRODUCTIVE IMMUNOLOGY. Palm Cove, AUSTRALIA: ELSEVIER IRELAND LTD. DOI
2010	Sharkey, D. J., & Robertson, S. A. (2010). Sperm and seminal plasma differentially regulate cytokine and chemokine protein expression by human cervical epithelial cells. Poster session presented at the meeting of JOURNAL OF REPRODUCTIVE IMMUNOLOGY. Palm Cove, AUSTRALIA: ELSEVIER IRELAND LTD. DOI

Patents

Year	Citation
2004	Robertson, S., Sharkey, D., Tremellen, K., & Glynn, D. (2004). WO/2004/026333, Treatment and diagnosis of a reproductive disorder by measuring or inhibiting interferon-&gammad. Australia.

Filesets

Year	Citation
	Nisenblat, V., Sharkey, D., Wang, Z., Evans, S., HEALEY, M., Ohlsson-Teague, E. M. C., . . . Hull, L. (n.d.). Supplementary Material from: Plasma microRNAs display limited potential as diagnostic tools for endometriosis. DOI

Competitive Research Funding

2010

Recipient of Research Centre for Reproductive Health, Skills Competency Training Award

Project: Influence of seminal fluid and Chlamydia trachomatis infection on endocervical
epithelial cell cytokine production in vitro.
(Research project and initiation of new collaboration with A/Prof Alison Quayle and
Dr Lyndsey Buckner at Louisiana State University Health Science Center, New Orleans, USA)

AUD $7,500

2009

Recipient of inaugural Robinson Research Institute Early Career Research Grant
Project: Is Activin A involved in the post-coital inflammatory response in the human cervix?

AUD $10,000

2008

Recipient of Research Centre for Reproductive Health, Colin Matthews

Early Career Research Grant

Project: The post-coital inflammatory response in the human cervix: A potential role for Follistatin?

AUD $10,000

Supervision of Research Students and teaching Experience

2011 - Present	Teaching of the “Data Management and Introduction to Laboratory Safety” and “Patenting Research - working through the process” tutorials into the Human Reproductive Health III, Research Project in Reproductive Health and Bachelor of Health Sciences Honours courses
2005 - Present	Co-supervisor of Honours students from the Robinson Research Institute and School of Paediatrics and Reproductive Health
2005 - Present	Supervision of Discipline of Obstetrics and Gynaecology Summer Vacation Research Scholars (4)
2000 - 2004	1^st and 2^nd year Medical Tutor and Examiner - Problem Based Learning, Department of Medicine, The University of Adelaide
2000	Microbiology and Immunology practical course demonstrator and assessor for 2^nd year Bachelor of Science students and 3^rd year Medical students

Current Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2021	Co-Supervisor	Novel molecular mediators of seminal fluid interaction with the female reproductive tract immune response	Doctor of Philosophy	Doctorate	Full Time	Miss Hannah Elizabeth Lyons
2020	Co-Supervisor	New insight into spermatozoa selection for improving assisted reproductive technologies (ARTs) outcomes	Doctor of Philosophy	Doctorate	Full Time	Mrs Soraya Rasi Ghaemi

Committee Memberships

Date	Role	Committee	Institution	Country
2019 - ongoing	Secretary	Society for Reproductive Biology Council	Society for Reproductive Biology	Australia
2019 - ongoing	Member	New Frontiers For A Healthy Start To Life Organising Committee	Robinson Research Institute, The University of Adelaide	Australia
2018 - ongoing	Member	American Society for Reproductive Immunology Program Organising Committee	American Society for Reproductive Immunology	United States
2015 - 2016	Member	Adelaide Medical and Nursing School – Look and Feel Workgroup
2010 - 2013	Member	School of Paediatrics and Reproductive Health – IP and Commercialisation Committee
2010 - 2012	Co-Chair	School of Paediatrics and Reproductive Health Research Seminar Series
2008 - 2012	Co-Chair	Robinson Research Institute ECR / MCR Professional Development Program
2008 - ongoing	Member	Australian Society for Medical Research
2007 - 2012	Representative	School of Paediatrics and Reproductive Health, Research Committee
2007 - 2012	Representative	School of Paediatrics and Reproductive Health
2007 - 2012	Representative	Discipline of Obstetrics and Gynaecology
2000 - 2002	Member	Australian Society for Medical Research

Editorial Boards

Date	Role	Editorial Board Name
2017 - ongoing	Editor	Frontiers in Endocrinology
2017 - ongoing	Editor	Frontiers in Physiology
2017 - ongoing	Editor	Frontiers in Physiology
2015 - ongoing	Editor	Journal of Reproductive Endocrinology and Infertility

Position: NHMRC Postdoctoral Researcher (B)
Phone: 83131503
Email: david.sharkey@adelaide.edu.au
Campus: North Terrace
Building: Adelaide Health and Medical Sciences, floor 6
Org Unit: Medical Sciences