Tara Pukala

Associate Professor Tara Pukala

Associate Professor

School of Physical Sciences

Faculty of Sciences

Eligible to supervise Masters and PhD, but is currently at capacity - email supervisor to discuss availability.

Tara Pukala obtained a PhD from the University of Adelaide in 2006, under the supervision of Prof John Bowie. This was followed by a postdoctoral position at the University of Cambridge, UK, working with Prof Dame Carol Robinson in the field of native mass spectrometry.

Tara returned to Australia to her current role as lecturer in the Discipline of Chemistry at the University of Adelaide in 2008. Here she leads a multidisciplinary research group focused on developing new approaches, primarily utilising mass spectrometry, to investigate the structure, function and interactions of macromolecules important in biology.

Since 2017 she has had the role of Scientific Director of the Adelaide Proteomics Centre.

Biological macromolecules such as proteins and DNA form the machinery that underlies all biological functions. The ability to ‘see’ the atomic detail of these assemblies, including their structures and interactions with other molecules, provides an opportunity to understand their biological activity. It also helps unravel processes which have gone astray to cause disease, and therefore find new treatments for a range of human illnesses.

In recent years mass spectrometry has emerged as a powerful tool for structural biology, in many cases capable of exceeding other techniques by diversity in application and information content. This is due to the ability of mass spectrometry to precisely identify biomolecules, binding interactions, detect individual species present in a mixture, and offer conformational information for large, dynamic and heterogeneous structures, with physiological relevance.

Research in the Pukala group is directed towards development of new approaches, primarily using mass spectrometry, to obtain insight into the structure, function and interactions of macromolecules, with a focus on enhancing understanding of multi-molecular systems involved in disease.

Current projects include;

  • development of ion mobility-mass spectrometry (and complementary tools) for the study of large protein complexes
  • design of novel mass spectrometry amenable protein cross-linking strategies
  • mechanistic studies of protein misfolding and aggregation
  • structural analysis of triplex DNA

For more details of current projects, see the Pukala Group website.

Australian Research Council
2020-22:  ARC Discovery Project (DP200102291)
T Pukala Revealing molecular detail of DNA triplexes to underpin antigene technology, $450K

2020: ARC LIEF (LE200100183)
P.Hoffmann, T. Chataway, T. Pukala, M. Herderich, E. Hilder,  D. Keating, K. Kirkbride, S. Plush, S. Pitson, C. Proud, D. Brooks. Protein quantitation centre of South Australia renewal for systems biology

2018-20:  ARC Discovery Project (DP180103974)
V Bulone and T Pukala.  Characterisation of vital carbohydrate synthases in pathogenic oomycetes, $546K 

2017-19:  ARC Discovery Project (DP170102033)
T Pukala and A Politis. Mass spectrometry to reveal the role of lipids in protein misfolding, $324K

2011-13:  ARC Discovery Project (DP110100496)
T Pukala. Structural and functional investigations of the human transcription machinery by ion mobility mass spectrometry, $340K

2010-12:  ARC Discovery Project (DP1093143)
T Pukala and J Bowie. Novel mass spectrometric approaches to the study of protein-protein interactions, $360K

2013:  ARC LIEF (LE130100199)
P Hoffmann, T Chataway, J Bowie, G Fincher, M Brown, T Pukala, C Franco, D Keating, D Brooks, L Cosgrove, K Fung, M Herderich, W Hines.  Establishment of the peptide and protein quantitation centre of South Australia: PAPQuaC-SA, $475K

2012:  ARC LIEF (LE120100181)
L Botten, D Leinweber, L Radom, J Gready, M England, A Mark, H Blackburn, A Hogg, C Jakob, A Pitman, BJ Powell, K Rasmussen, L Moresi, A Yu, M Knackstedt, M Bilek, M Ragan, B Schmidt, R Muller, T Pukala, J Sori, C Turney, K Hourigan, D Adelson, R Khanna, J Denier, B Pailthorpe. Strengthened merit-based access and support at the new NCI petascale supercomputing facility, $650K

Other
2019-2022: Department of Industry and Skills Research Consortia Program (FND000639)
Bulone V, Pukala T, Abell A, Zannettino A, Beattie D, Krasowska M, Zhou Q, Koltunow A. Research Consortium Program for Agricultural Product Development, $4M

2016-2018:  The Australia & Pacific Science Foundation
S Petit, T Pukala, R Schumann, A Naikatini, A Scanlon. Unique pollination system of an important rainforest tree involving obligate manipulation by bats, $45K

2016-17: University of Adelaide Interdisciplinary Research Fund
V Bulone, T Pukala, A Zannettino, C Doonan, C Sumby. An interdisciplinary research program to map the protein-glycan interactome of key eukaryotic cellular systems: interfacing biophysics and chemistry with cancer research, microbiology and plant sciences, $25K

2016: University of Adelaide Faculty of Sciences eLearning Program
NM Williamson, TL Pukala, CJ Doonan.  Development of level II chemistry online assessment and student feedback. $7K

2013-14: University of Adelaide Interdisciplinary Research Fund
I Musgrave, S Smid, L O’Keefe, T Pukala. Structure function relationships of antiamyloid drugs: verification in an in-vivo model, $21K

2013-14: University of Adelaide Interdisciplinary Research Fund
T Pukala and M Fuller. Mass spectrometric approaches to characterise alpha-synuclein-glucosylsphingosine interations: application to the Parkinsonian Syndrome, $25K

Teaching interests include:

Biological structure determination
Mass spectrometry and NMR spectroscopy
Biological and synthetic organic chemistry
Physical organic chemistry
Foundations of chemistry

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  • Current Higher Degree by Research Supervision (University of Adelaide)
    Date Role Research Topic Program Degree Type Student Load Student Name
    2020 Co-Supervisor physical and chemical properties of encapsulin proteins Doctor of Philosophy Doctorate Full Time Mr Benjamin James Lethbridge
    2020 Principal Supervisor Chemical and Functional Exploration of Agricultural Waste for Identification of High Value Bioactives Master of Philosophy Master Full Time Mr River Jack Pachulicz
    2020 Co-Supervisor Developing synchrotron imaging technology to address questions in redox biology. Doctor of Philosophy Doctorate Full Time Mr Jake William Andersen
    2020 Principal Supervisor Structural characterisation of snake venom proteins by mass spectrometry Doctor of Philosophy Doctorate Full Time Miss Chia-De Ruth Wang
    2020 Principal Supervisor Biophysical characterisation of DNA triplexes for antigene technology Doctor of Philosophy Doctorate Full Time Mr Jack Klose
    2019 Principal Supervisor Mass Spectrometric Characterisation of Protein Assemblies from Snake Venom Master of Philosophy Master Full Time Miss Emily Rose Bubner
    2019 Co-Supervisor Identification of Human Biomarkers for Early Detection of Alzheimer's Disease ad other Dementia Related Illnesses Doctor of Philosophy Doctorate Full Time Ms Kym McNicholas
    2017 Principal Supervisor Synthesis and application of novel protein reagents for structure determination. Doctor of Philosophy Doctorate Full Time Miss Katherine Stevens
    2017 Principal Supervisor Biophysical Characterisation of unusual DNA Structures Doctor of Philosophy Doctorate Full Time Mr Alexander Jacob Begbie
    2016 Principal Supervisor Characterisation of proteins and protein complexes using MS Doctor of Philosophy Doctorate Part Time Mr Henry Michael Sanders
    2015 Principal Supervisor Analysis of In-Vitro Aggregation of Therapeutic Proteins Using immobility Mass Spectrometry Doctor of Philosophy Doctorate Part Time Mr Paritosh Kumar Tripathi

  • Past Higher Degree by Research Supervision (University of Adelaide)
    Date Role Research Topic Program Degree Type Student Load Student Name
    2018 - 2020 Principal Supervisor Structural Investigation of Snake Venom Proteins by Mass Spectrometry Master of Philosophy Master Full Time Miss Chia-De Ruth Wang
    2016 - 2018 Co-Supervisor A Mechanistic Study of the Stability of Triple-Stranded DNA Structures Master of Philosophy Master Full Time Mrs Belinda Jayne Boehm
    2015 - 2018 Co-Supervisor Small molecular inhibitors of Amyloid beta and alpha Synuclein amyloidogenic aggregation, toxicity and in silico design of amyloid-binding ligands Doctor of Philosophy Doctorate Full Time Mrs Sukanya Das
    2014 - 2017 Principal Supervisor Design and Synthesis of Protein Chemical Crosslinkers: A Modular Approach Master of Philosophy Master Full Time Miss Kayla Monique Downey
    2012 - 2018 Principal Supervisor Biological and Structure Characterisation of Eukaryotic Prefoldin Doctor of Philosophy Doctorate Full Time Denise Tran
    2011 - 2019 Co-Supervisor Application of Synchrotron-Based Spectroscopic Techniques XAS and XFM towards Probing the Cellular Metallome Doctor of Philosophy Doctorate Full Time Mr Jason Lee Wedding
    2010 - 2014 Co-Supervisor Structural and mechanistic studies of post-translationally modified peptides and proteins Doctor of Philosophy Doctorate Full Time Miss Thi Thanh Nha Tran
    2009 - 2013 Co-Supervisor Characterisation of Protein Structure and Interactions: Novel Applications to the Study of Bioactive Peptides Doctor of Philosophy Doctorate Full Time Antonio Calabrese
    2009 - 2014 Co-Supervisor Linked beta-cyclodextrin trimers: From molecular recognition to polymer network hydrogels Doctor of Philosophy Doctorate Full Time Mrs Trang Thi Hanh Nguyen
    2009 - 2014 Co-Supervisor A study on the interactions of synthetic IGF-II analogues with the type 1 IGF and insulin receptors Doctor of Philosophy Doctorate Part Time Miss Jade Misty Cottam
  • Position: Associate Professor
  • Phone: 83135497
  • Email: tara.pukala@adelaide.edu.au
  • Fax: 8313 4380
  • Campus: North Terrace
  • Building: Badger, floor 2
  • Room: 2 22
  • Org Unit: Chemistry
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