Pirjo Apaja

School of Biological Sciences

Faculty of Sciences, Engineering and Technology

Eligible to supervise Masters and PhD - email supervisor to discuss availability.


A/Prof Apaja is a principal investigator of Organelle Proteostasis Diseases laboratory at the Molecular and Biomedical Sciences and South Australian Health and Medical Research Institute.

Physiological conditions such as ageing, inflammation, stress insults as well as many brain diseases, cancers and numerous Mendelian diseases are directly driven or cause a short or long-term dysbalance in protein homeostasis.

Our research is focused on finding ways to reprogramme Protein Homeostasis (Proteostasis) Stress in human diseases, specifically through cellular disposal and surveillance systems to adapt to changing physiological conditions.

We are focusing on identifying key proteostasis networks, compounds and post-translational modifications in transport, recognition and disposal of membrane organelle/protein targets in neurodegenerative and -developmental diseases and cancers.

My laboratory combines screenings, omics and protein networks, testing of small molecule compounds, protein and membrane organelle microscopy with other biochemical and cell biological approaches in disease models.

 

  • Journals

    Year Citation
    2023 Rashidieh, B., Bain, A. L., Tria, S. M., Sharma, S., Stewart, C. A., Simmons, J. L., . . . Khanna, K. K. (2023). Alpha-B-Crystallin overexpression is sufficient to promote tumorigenesis and metastasis in mice. Experimental Hematology & Oncology, 12(1), 1-19.
    DOI
    2022 Wang, B. B., Xu, H., Isenmann, S., Huang, C., Elorza-Vidal, X., Rychkov, G. Y., . . . Apaja, P. M. (2022). Ubr1-induced selective endophagy/autophagy protects against the endosomal and Ca²⁺-induced proteostasis disease stress. Cellular and molecular life sciences : CMLS, 79(3), 167-1-167-22.
    DOI
    2022 Wang, B. B., & Apaja, P. M. (2022). Reprogramming endo-lysosomal proteostasis disease stress by UBR1- and arginylation-driven endophagy and autophagy protein quality control. Autophagy Reports, 1(1), 260-263.
    DOI
    2021 Xu, H., Isenmann, S., López-Hernández, T., Estévez, R., Lukacs, G. L., & Apaja, P. M. (2021). Control of membrane protein homeostasis by a chaperone-like glial cell adhesion molecule at multiple subcellular locations. Scientific Reports, 11(1), 17 pages.
    DOI Scopus3 WoS3 Europe PMC2
    2021 Wang, B. B., Xu, H., Isenmann, S., Huang, C., Elorza-Vidal, X., Rychkov, G. Y., . . . Apaja, P. M. (2021). Ubr1-induced selective endo-phagy/autophagy protects against the endosomal and Ca2+-induced proteostasis disease stress.
    DOI
    2021 Kazan, J. M., Desrochers, G., Martin, C. E., Jeong, H., Kharitidi, D., Apaja, P. M., . . . Pause, A. (2021). Endofin is required for HD-PTP and ESCRT-0 interdependent endosomal sorting of ubiquitinated transmembrane cargoes. iScience, 24(11), 27 pages.
    DOI Scopus3 WoS2 Europe PMC3
    2019 Kazan, J. M., Lukacs, G. L., Apaja, P. M., & Pause, A. (2019). Single Cell Fluorescence Ratio Image Analysis for Studying ESCRT Function in Receptor Trafficking. Methods in molecular biology (Clifton, N.J.), 1998, 93-103.
    DOI Scopus3 Europe PMC4
    2018 Okiyoneda, T., Veit, G., Sakai, R., Aki, M., Fujihara, T., Higashi, M., . . . Lukacs, G. L. (2018). Chaperone-Independent Peripheral Quality Control of CFTR by RFFL E3 Ligase. Developmental Cell, 44(6), 694-708.e7.
    DOI Scopus33 WoS35 Europe PMC28
    2017 Bagdany, M., Veit, G., Fukuda, R., Avramescu, R. G., Okiyoneda, T., Baaklini, I., . . . Lukacs, G. L. (2017). Chaperones rescue the energetic landscape of mutant CFTR at single molecule and in cell. Nature Communications, 8(1), 15 pages.
    DOI Scopus44 WoS46 Europe PMC37
    2017 Gaitán-Peñas, H., Apaja, P., Arnedo, T., Castellanos, A., Elorza-Vidal, X., Soto, D., . . . Estévez, R. (2017). Leukoencephalopathy-causing CLCN2 mutations are associated with impaired Cl− channel function and trafficking. Journal of Physiology, 595(22), 6993-7008.
    DOI Scopus19 WoS20 Europe PMC17
    2017 Hein, L., Apaja, P., Hattersley, K., Grose, R., Xie, J., Proud, C., & Sargeant, T. (2017). A novel fluorescent probe reveals starvation controls the commitment of amyloid precursor protein to the lysosome. Biochimica et Biophysica Acta - Molecular Cell Research, 1864(10), 1554-1565.
    DOI Scopus15 WoS14 Europe PMC8
    2016 Veit, G., Oliver, K., Apaja, P., Perdomo, D., Bidaud-Meynard, A., Lin, S., . . . Lukacs, G. (2016). Ribosomal stalk protein silencing partially corrects the ΔF508-CFTR functional expression defect. PLoS Biology, 14(5), e1002462-1-e1002462-32.
    DOI Scopus37 Europe PMC35
    2016 Veit, G., Oliver, K., Apaja, P. M., Perdomo, D., Bidaud-Meynard, A., Lin, S. -T., . . . Lukacs, G. L. (2016). Ribosomal Stalk Protein Silencing Partially Corrects the Delta F508-CFTR Functional Expression Defect. PLOS BIOLOGY, 14(5), 32 pages.
    DOI WoS38
    2015 Kharitidi, D., Apaja, P. M., Manteghi, S., Suzuki, K., Malitskaya, E., Roldan, A., . . . Pause, A. (2015). Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration. Cell Reports, 13(3), 599-609.
    DOI Scopus35 WoS34 Europe PMC30
    2014 Veit, G., Avramescu, R. G., Perdomo, D., Phuan, P. W., Bagdany, M., Apaja, P. M., . . . Lukacs, G. L. (2014). Cystic fibrosis: Some gating potentiators, including VX-770, diminish ΔF508-CFTR functional expression. Science Translational Medicine, 6(246), 13 pages.
    DOI Scopus236 WoS234 Europe PMC151
    2014 Chu, C., King, J., Berrini, M., Rumley, A., Apaja, P., Lukacs, G., . . . Cordat, E. (2014). Degradation mechanism of a Golgi-retained distal renal tubular acidosis mutant of the kidney anion exchanger 1 in renal cells. American Journal of Physiology - Cell Physiology, 307(3), C296-C307.
    DOI Scopus12 WoS11 Europe PMC9
    2014 Apaja, P. M., & Lukacs, G. L. (2014). Protein homeostasis at the plasma membrane. Physiology, 29(4), 265-277.
    DOI Scopus32 WoS27 Europe PMC24
    2013 Apaja, P. M., Foo, B., Okiyoneda, T., Valinsky, W. C., Barriere, H., Atanasiu, R., . . . Shrier, A. (2013). Ubiquitination-dependent quality control of hERG K+ channel with acquired and inherited conformational defect at the plasma membrane. Molecular Biology of the Cell, 24(24), 3787-3804.
    DOI Scopus33 WoS31 Europe PMC30
    2013 Capdevila-Nortes, X., López-Hernández, T., Apaja, P. M., de Heredia, M. L., Sirisi, S., Callejo, G., . . . Estévez, R. (2013). Insights into MLC pathogenesis: GlialCAM is an MLC1 chaperone required for proper activation of volume-regulated anion currents. Human Molecular Genetics, 22(21), 4405-4416.
    DOI Scopus40 WoS41 Europe PMC33
    2012 Apaja, P. M., Okiyondea, T., Barriere, H., Lam, H., Atanasiu, R., Foo, B., . . . Shrier, A. (2012). hERG Quality Control at the Plasma Membrane. BIOPHYSICAL JOURNAL, 102(3), 677A.
    DOI
    2011 Okiyoneda, T., Apaja, P. M., & Lukacs, G. L. (2011). Protein quality control at the plasma membrane. Current Opinion in Cell Biology, 23(4), 483-491.
    DOI Scopus62 WoS60 Europe PMC55
    2011 Barrière, H., Apaja, P., Okiyoneda, T., & Lukacs, G. L. (2011). Endocytic Sorting of CFTR Variants Monitored by Single-Cell Fluorescence Ratiometric Image Analysis (FRIA) in Living Cells. Methods in molecular biology (Clifton, N.J.), 741, 301-317.
    DOI Scopus11 Europe PMC9
    2010 Apaja, P. M., Xu, H., & Lukacs, G. L. (2010). Quality control for unfolded proteins at the plasma membrane. Journal of Cell Biology, 191(3), 553-570.
    DOI Scopus49 WoS48 Europe PMC43
    2007 Tuusa, J. T., Markkanen, P. M. H., Apaja, P. M., Hakalahti, A. E., & Petäjä-Repo, U. E. (2007). The Endoplasmic Reticulum Ca<sup>2+</sup>-pump SERCA2b Interacts with G Protein-coupled Receptors and Enhances their Expression at the Cell Surface. Journal of Molecular Biology, 371(3), 622-638.
    DOI Scopus16 WoS15 Europe PMC13
    2006 Apaja, P. M., Tuusa, J. T., Pietilä, E. M., Rajaniemi, H. J., & Petäjä-Repo, U. E. (2006). Luteinizing hormone receptor ectodomain splice variant misroutes the full-length receptor into a subcompartment of the endoplasmic reticulum. Molecular Biology of the Cell, 17(5), 2243-2255.
    DOI Scopus44 WoS42 Europe PMC36
    2005 Pietilä, E. M., Tuusa, J. T., Apaja, P. M., Aatsinki, J. T., Hakalahti, A. E., Rajaniemi, H. J., & Petäjä-Repo, U. E. (2005). Inefficient maturation of the rat luteinizing hormone receptor: A putative way to regulate receptor numbers at the cell surface. Journal of Biological Chemistry, 280(28), 26622-26629.
    DOI Scopus65 WoS62 Europe PMC50
    2005 Apaja, P. M., Aatsinki, J. T., Rajaniemi, H. J., & Petäjä-Repo, U. E. (2005). Expression of the mature luteinizing hormone receptor in rodent urogenital and adrenal tissues is developmentally regulated at a posttranslational level. Endocrinology, 146(8), 3224-3232.
    DOI Scopus38 WoS31 Europe PMC22
    2004 Apaja, P. M., Harju, K. T., Aatsinki, J. T., Petäjä-Repo, U. E., & Rajaniemi, H. J. (2004). Identification and Structural Characterization of the Neuronal Luteinizing Hormone Receptor Associated with Sensory Systems. Journal of Biological Chemistry, 279(3), 1899-1906.
    DOI Scopus74 WoS60 Europe PMC44
  • Conference Papers

    Year Citation
    2014 Foo, B., Apaja, P., Okiyoneda, T., Barriere, H., Ficker, E., Lukacs, G., & Shrier, A. (2014). A peripheral protein quality control machinery involved in acquired and inherited LQT syndrome. In BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE Vol. 92 (pp. 581). CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS.
    2014 Avramescu, R. G., Perdomo, D., Phuan, P. W., Bagdany, M., Apaja, P. M., Borot, F., . . . Veit, G. (2014). Exposure to some gating potentiators, including VX-770, diminishes dF508-CFTR correction efficiency. In BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE Vol. 92 (pp. 580). CANADIAN SCIENCE PUBLISHING, NRC RESEARCH PRESS.
    2010 Lukacs, G. L., & Apaja, P. (2010). Membrane protein quality control in post-Golgi compartments. In BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE Vol. 88 (pp. 406). NATL RESEARCH COUNCIL CANADA-N R C RESEARCH PRESS.
    2009 Apaja, P., & Lukacs, G. L. (2009). Membrane protein quality control in post-Golgi compartments. In FASEB JOURNAL Vol. 23 (pp. 1 page). FEDERATION AMER SOC EXP BIOL.
  • Conference Items

    Year Citation
    2015 Veit, G., Oliver, K. E., Apaja, P. M., Perdomo, D., Lin, S., Guo, J., . . . Lukacs, G. L. (2015). RIBOSOMAL STALK PROTEIN SILENCING CORRECTS THE Delta F508-CFTR FUNCTIONAL EXPRESSION DEFECT. Poster session presented at the meeting of PEDIATRIC PULMONOLOGY. WILEY-BLACKWELL.
    2014 Veit, G., Avramescu, R. G., Perdomo, D., Phuan, P., Bagdany, M., Apaja, P. M., . . . Lukacs, G. L. (2014). SOME GATING POTENTIATORS, INCLUDING VX-770, DIMINISH Delta F508-CFTR FUNCTIONAL EXPRESSION. Poster session presented at the meeting of PEDIATRIC PULMONOLOGY. WILEY-BLACKWELL.

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