Professor Maria Hrmova

Maria Hrmova
Professor
School of Agriculture, Food and Wine
Faculty of Sciences

Research in my laboratory is focused on membrane transporters and transcription factors involved in abiotic stress tolerance of plants. Our interests are also centred on enzymes that participate in formation of plant cell surface structures. Further, we have created artificial membrane environments mimicking native ones using bicelles, liposomes, lipodiscs and nanodiscs, and used them for functional reconstitution of membrane proteins produced through cell-based and cell-free synthesis platforms. The goals of our projects are to address the fundamental questions as to how proteins mediate their biological functions. The new knowledge is expected to serve for designing of engineered proteins that work optimally under a variety of abiotic stress factors.

Professor Maria Hrmova

Research in my laboratory is focused on membrane transporters and transcription factors involved in abiotic stress tolerance of plants. Our interests are also centred on enzymes that participate in formation of plant cell surface structures. Further, we have created artificial membrane environments mimicking native ones using bicelles, liposomes, lipodiscs and nanodiscs, and used them for functional reconstitution of membrane proteins produced through cell-based and cell-free synthesis platforms. The goals of our projects are to address the fundamental questions as to how proteins mediate their biological functions. The new knowledge is expected to serve for designing of engineered proteins that work optimally under a variety of abiotic stress factors.

My expertise is in the multidisciplinary field of structural biology. I research the molecular mechanisms of plant proteins in the three main areas:

TRANSPORT PROTEINS INVOLVED IN BORON TOXICITY TOLERANCE AND SALINITY - My laboratory is focussed on structural and functional analyses of plant borate and HKT Na+/K+ transporters, and aquaporins. By combining in silico and in vitro methods, we discovered that borate transporters mediate Na+-dependent anion transport and exhibit channel-like characteristics. This work was extended into an innovative approach of measuring permeation of transporters using electrochemical impedance spectroscopy. We designed a model for Na+-exclusion in rice, and explained that variations in tolerance can be explained by transcription, alternative splicing, and a HKT transporter structure. Further, we develop protein production methods for plant membrane proteins based on eukaryotic cell-free synthesis, and create the membrane mimicking environments of bicelles, liposomes, lipodiscs and nanodiscs for membrane protein reconstitution. We have reconstituted membrane proteins in these environments, and imaged them through atomic force microscopy and small angle X-ray scattering using synchrotron radiation.

TRANSCRIPTION FACTORS INVOLVED IN THE REGULATION OF PLANT RESPONSES TO DROUGHT - We perform extensive molecular modelling studies of transcription factors in complex with DNA cis-elements, such as bZIP, HDZip, DREB, ERF, NFY-YB, CBF and MYB. We described the molecular mechanisms of salt-sensitive SOS3 and lipid-binding proteins, and defensins. We engineered wheat transcription factor variants and in breakthrough genetic science using transgenic plants, we showed that these mutations change DNA recognition and plant responses to abiotic stresses, such as drought and frost. Some of our work was highlighted on the front covers of Plant Biotechnology Journal, Plant Moleculer Biology and Journal of Experimental Botany.

ENZYMES INVOLVED IN PLANT DEVELOPMENT - We focus on hydrolytic enzymes to define in atomic details their catalytic mechanisms, thermodynamic and structural determinants of substrate recognition, and natures of transition states that take part during a hydrolytic cycle. We crystallised a plant exohydrolase enzyme and solved its crystal structure, which represents the first atomic structure of this type of hydrolases. We solved more than forty structures of an exohydrolase in complex with mechanism-based inhibitors, substrate analogues and transition-state mimics. We investigated the structural basis of the product and substrate trajectory in the active site of the exohydrolase using X-ray crystallography and NMR spectroscopy. We use molecular dynamics and quantum mechanical simulations, to elucidate kinetic aspects of catalysis and substrate specificity of enzymes.

Appointments

Date Position Institution name
2010 Professor (Research Only) University of Adelaide

Awards and Achievements

Date Type Title Institution Name Amount
2012 Elected a member of the Learned Society of Slovak Academy of Sciences, Slovak Republic Comenius University

Education

Date Institution name Country Title
1978 - 1981 Comenius University in Bratislava Slovakia PhD

Certifications

Date Title Institution name Country
2012 Doctor Scientiarum Comenius University in Bratislava Slovakia

Journals

Year Citation
2017 BI, H., Luang, S., Bazanova, N., Borisjuk, N., Hrmova, M. & Lopato, S. (2017). Wheat drought-responsive WXPL transcription factors regulate cuticle biosynthesis genes. Plant Molecular Biology, 94, 1, 15-32. 10.1007/s11103-017-0585-9
2016 Rabiger, D., Taylor, J., Spriggs, A., Hand, M., Henderson, S., Johnson, S. ... Koltunow, A. (2016). Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation. BMC Biology, 14, 1, 86-1-86-21. 10.1186/s12915-016-0311-0
2016 Sornaraj, P., Luang, S., Lopato, S. & Hrmova, M. (2016). Basic leucine zipper (bZIP) transcription factors involved in abiotic stresses: a molecular model of a wheat bZIP factor and implications of its structure in function. Biochimica et Biophysica Acta, 1860, 1, Part A, 46-56. 10.1016/j.bbagen.2015.10.014
2016 Kovalchuk, N., Chew, W., Sornaraj, P., Borisjuk, N., Yang, N., Singh, R. ... Lopato, S. (2016). The homeodomain transcription factor TaHDZipI-2 from wheat regulates frost tolerance, flowering time and spike development in transgenic barley. New Phytologist, 211, 2, 671-687. 10.1111/nph.13919
2016 Nagarajan, Y., Rongala, J., Luang, S., Singh, A., Shadiac, N., Hayes, J. ... Hrmova, M. (2016). A barley efflux transporter operates in a Na⁺-dependent manner, as revealed by a multidisciplinary platform. The Plant Cell, 28, 1, 202-218. 10.1105/tpc.15.00625
2016 Zieleniecki, J., Nagarajan, Y., Waters, S., Rongala, J., Thompson, V., Hrmova, M. & Köper, I. (2016). Cell-free synthesis of a functional membrane transporter into a tethered bilayer lipid membrane. Langmuir, 32, 10, 2445-2449. 10.1021/acs.langmuir.5b04059
2016 Li, B., Byrt, C., Qiu, J., Baumann, U., Hrmova, M., Evrard, A. ... Roy, S. (2016). Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis. Plant Physiology, 170, 2, 1014-1029. 10.1104/pp.15.01163
2016 Amalraj, A., Luang, S., Kumar, M., Sornaraj, P., Eini, O., Kovalchuk, N. ... Lopato, S. (2016). Change of function of the wheat stress-responsive transcriptional repressor TaRAP2.1L by repressor motif modification. Plant Biotechnology Journal, 14, 2, 820-832. 10.1111/pbi.12432
2016 Bi, H., Luang, S., Li, Y., Bazanova, N., Morran, S., Song, Z. ... Lopato, S. (2016). Identification and characterization of wheat drought-responsive MYB transcription factors involved in the regulation of cuticle biosynthesis. Journal of Experimental Botany, 67, 18, 5363-5380. 10.1093/jxb/erw298
2016 Harris, J., Sornaraj, P., Taylor, M., Bazanova, N., Baumann, U., Lovell, B. ... Hrmova, M. (2016). Molecular interaction of the γ-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit. Plant Molecular Biology, 90, 4, 435-452. 10.1007/s11103-015-0427-6
2015 Tankrathok, A., Iglesias-Fernández, J., Williams, R., Pengthaisong, S., Baiya, S., Hakki, Z. ... Ketudat Cairns, J. (2015). A single glycosidase harnesses different pyranoside ring transition state conformations for hydrolysis of mannosides and glucosides. ACS Catalysis, 5, 10, 6041-6051. 10.1021/acscatal.5b01547
2015 Yadav, D., Shavrukov, Y., Bazanova, N., Chirkova, L., Borisjuk, N., Kovalchuk, N. ... Lopato, S. (2015). Constitutive overexpression of the TaNF-YB4 gene in transgenic wheat significantly improves grain yield. Journal of Experimental Botany, 66, 21, 6635-6650. 10.1093/jxb/erv370
2015 Zhang, H., Luo, M., Day, R., Talbot, M., Ivanova, A., Ashton, A. ... Koltunow, A. (2015). Developmentally regulated HEART STOPPER, a mitochondrially targeted L18 ribosomal protein gene, is required for cell division, differentiation, and seed development in Arabidopsis.. Journal of Experimental Botany, 66, 19, 5867-5880. 10.1093/jxb/erv296
2014 Lopato, S., Borisjuk, N., Langridge, P. & Hrmova, M. (2014). Endosperm transfer cell-specific genes and proteins: structure, function and applications in biotechnology. Frontiers in Plant Science, 5, FEB, 64-1-64-14. 10.3389/fpls.2014.00064
2014 Li, M., Lopato, S., Hrmova, M., Pickering, M., Shirley, N., Koltunow, A. & Langridge, P. (2014). Expression patterns and protein structure of a lipid transfer protein END1 from Arabidopsis. Planta, 240, 6, 1319-1334. 10.1007/s00425-014-2155-6
2014 Borisjuk, N., Hrmova, M. & Lopato, S. (2014). Transcriptional regulation of cuticle biosynthesis. Biotechnology Advances, 32, 2, 526-540. 10.1016/j.biotechadv.2014.01.005
2013 Waters, S., Gilliham, M. & Hrmova, M. (2013). Plant High-Affinity Potassium (HKT) transporters involved in salinity tolerance: structural insights to probe differences in ion selectivity. International Journal of Molecular Sciences, 14, 4, 7660-7680. 10.3390/ijms14047660
2013 Tankrathok, A., Iglesias-Ferna, J., Luang, S., Robinson, R., Kimura, A., Rovira, C. ... Cairns, J. (2013). Structural analysis and insights into the glycon specificity of the rice GH1 Os7BGlu26 β-D-mannosidase. Acta Crystallographica Section D-Biological Crystallography, 69, 10, 2124-2135. 10.1107/S0907444913020568
2013 Eini Gandomani, O., Yang, N., Pyvovarenko, T., Pillman, K., Bazanova, N., Tikhomirov, N. ... Lopato, S. (2013). Complex regulation by Apetala2 domain-containing transcription factors revealed through analysis of the stress-responsive TdCor410b promoter from durum wheat. PLoS One, 8, 3, 1-19. 10.1371/journal.pone.0058713
2013 Chew, W., Hrmova, M. & Lopato, S. (2013). Role of homeodomain leucine zipper (HD-Zip) iv transcription factors in plant development and plant protection from deleterious environmental factors. International Journal of Molecular Sciences, 14, 4, 8122-8147. 10.3390/ijms14048122
2013 Shadiac, N., Nagarajan, Y., Waters, S. & Hrmova, M. (2013). Close allies in membrane protein research: cell-free synthesis and nanotechnology. Molecular Membrane Biology, 30, 3, 229-245. 10.3109/09687688.2012.762125
2013 Periasamy, A., Shadiac, N., Amalraj, A., Garajova, S., Nagarajan, Y., Waters, S. ... Hrmova, M. (2013). Cell-free protein synthesis of membrane (1,3)-beta-D-glucan (curdlan) synthase: Co-translational insertion in liposomes and reconstitution in nanodiscs. Biochimica et Biophysica Acta-Biomembranes, 1828, 2, 743-757. 10.1016/j.bbamem.2012.10.003
2012 Marti, A., Wirthensohn, M., Alonso, J., Company, R. & Hrmova, M. (2012). Molecular modeling of S-RNases involved in almond self-incompatibility. Frontiers in Plant Science, 3, 139, 1-4. 10.3389/fpls.2012.00139
2012 Kovalchuk, N., Gilson, J., Bazanova, N., Pyvovarenko, T., Singh, R., Shirley, N. ... Lopato, S. (2012). Characterization of the wheat gene encoding a grain-specific lipid transfer protein TdPR61, and promoter activity in wheat, barley and rice. Journal of Experimental Botany, 63, 5, 2025-2040. 10.1093/jxb/err409
2012 Cotsaftis, O., Plett, D., Shirley, N., Tester, M. & Hrmova, M. (2012). A two-staged model of Na+ exclusion in rice explained by 3D modeling of HKT transporters and alternative splicing. PLoS One, 7, 7, 1-10. 10.1371/journal.pone.0039865
2011 Harris, J., Hrmova, M., Lopato, S. & Langridge, P. (2011). Modulation of plant growth by HD-Zip class I and II transcription factors in response to environmental stimuli. New Phytologist - Online, 190, 4, 1-15. 10.1111/j.1469-8137.2011.03733.x
2011 Marti, A., Alonso, J., Company, R., Wirthensohn, M. & Hrmova, M. (2011). Molecular modelling of RNases from almond involved in self-incompatibility. B. E. Ak, M. Wirthensohn & T. Gradziel (Eds.). Proceedings of the Vth International Symposium on Pistachios and Almonds, 912, 641-643.
2011 Rivandi, A., Miyazaki, J., Hrmova, M., Pallotta, M., Tester, M. & Collins, N. (2011). A SOS3 homologue maps to HvNax4, a barley locus controlling an environmentally sensitive Na⁺ exclusion trait. Journal of Experimental Botany, 62, 3, 1201-1216. 10.1093/jxb/erq346
2011 Drew, D., Hrmova, M., Lunde, C., Jacobs, A., Tester, M. & Fincher, G. (2011). Structural and functional analyses of PpENA1 provide insights into cation binding by type IID P-type ATPases in lower plants and fungi. Biochimica et Biophysica Acta-Biomembranes, 1808, 6, 1483-1492. 10.1016/j.bbamem.2010.11.013
2010 Vaaj-Kolstad, G., Farkas, V., Fincher, G. & Hrmova, M. (2010). Barley xyloglucan xyloglucosyl transferases bind xyloglucan-derived oligosaccharides in their acceptor-binding regions in multiple conformational states. Archives of Biochemistry and Biophysics, 496, 1, 61-68. 10.1016/j.abb.2010.01.011
2010 Hanlin, R., Hrmova, M., Harbertson, J. & Downey, M. (2010). Review: Condensed tannin and grape cell wall interactions and their impact on tannin extractability into wine. Australian Journal of Grape and Wine Research, 16, 1, 173-188. 10.1111/j.1755-0238.2009.00068.x
2010 Kuntothom, T., Raab, M., Tvaroska, I., Fort, S., Pengthaisong, S., Canada, J. ... Hrmova, M. (2010). Binding of β-D-glucosides and β-D-mannosides by rice and barley β-D-glycosidases with distinct substrate specificities. Biochemistry, 49, 40, 8779-8793. 10.1021/bi101112c
2010 Hrmova, M., Stone, B. & Fincher, G. (2010). High-yield production, refolding and a molecular modelling of the catalytic module of (1,3)-β-D-glucan (curdlan) synthase from Agrobacterium sp.. Glycoconjugate Journal, 27, 4, 461-476. 10.1007/s10719-010-9291-4
2010 Kosik, O., Auburn, R., Russell, S., Stratilova, E., Garajova, S., Hrmova, M. & Farkas, V. (2010). Polysaccharide microarrays for high-throughput screening of transglycosylase activities in plant extracts. Glycoconjugate Journal, 27, 1, 79-87. 10.1007/s10719-009-9271-8
2010 Vaaje-Kolstad, G., Farkas, V., Hrmova, M. & Fincher, G. (2010). Xyloglucan xyloglucosyl transferases from barley (Hordeum vulgare L.) bind oligomeric and polymeric xyloglucan molecules in their acceptor binding sites. Biochimica et Biophysica Acta-General Subjects, 1800, 7, 674-684. 10.1016/j.bbagen.2010.04.001
2010 Kovalchuk, N., Li, M., Wittek, F., Reid, N., Singh, R., Shirley, N. ... Lopato, S. (2010). Defensin promoters as potential tools for engineering disease resistance in cereal grains. Plant Biotechnology Journal, 8, 1, 47-64. 10.1111/j.1467-7652.2009.00465.x
2010 Kaiser, B. & Hrmova, M. (2010). A glimpse at regulation of nitrogen homeostasis. Structure, 18, 11, 1395-1397. 10.1016/j.str.2010.10.003
2010 Luang, S., Hrmova, M. & Cairns, J. (2010). High-level expression of barley β-d-glucan exohydrolase HvExoI from a codon-optimized cDNA in Pichia pastoris. Protein Expression and Purification, 73, 1, 90-98. 10.1016/j.pep.2010.04.011
2010 Kaewthai, N., Harvey, A., Hrmova, M., Brumer, H., Ezcurra, I., Teeri, T. & Fincher, G. (2010). Heterologous expression of diverse barley XTH genes in the yeast Pichia pastoris. Plant Biotechnology, 27, 3, 251-258. 10.5511/plantbiotechnology.27.251
2010 Schnurbusch, T., Hayes, J., Hrmova, M., Baumann, U., Ramesh, S., Tyerman, S. ... Sutton, T. (2010). Boron toxicity tolerance in barley through reduced expression of the multifunctional aquaporin HvNIP2;1. Plant Physiology, 153, 4, 1706-1715. 10.1104/pp.110.158832
2010 Luang, S., Cairns, J., Streltsov, V. & Hrmova, M. (2010). Crystallisation of wild-type and variant forms of a recombinant plant enzyme β-D-glucan glucohydrolase from barley (Hordeum vulgare L.) and preliminary X-ray analysis. International Journal of Molecular Sciences (Online), 11, 7, 2759-2769. 10.3390/ijms11072759
2010 Zhang, Q., Shirley, N., Burton, R., Lahnstein, J., Hrmova, M. & Fincher, G. (2010). The genetics, transcriptional profiles, and catalytic properties of UDP-α-D-xylose 4-epimerases from barley. Plant Physiology, 153, 2, 555-568. 10.1104/pp.110.157644
2009 Pettolino, F., Sasaki, I., Turbic, A., Wilson, S., Bacic, A., Hrmova, M. & Fincher, G. (2009). Hyphal cell walls from the plant pathogen Rhynchosporium secalis contain (1,3/1,6)-β-D-glucans, galacto- and rhamnomannans, (1,3;1,4)-β-D-glucans and chitin. FEBS Journal, 276, 14, 3698-3709. 10.1111/j.1742-4658.2009.07086.x
2009 Hrmova, M., Farkas, V., Harvey, A., Lahnstein, J., Wischmann, B., Kaewthai, N. ... Fincher, G. (2009). Substrate specificity and catalytic mechanism of a xyloglucan xyloglucosyl transferase HvXET6 from barley (Hordeum vulgare L.). FEBS Journal, 276, 2, 437-456. 10.1111/j.1742-4658.2008.06791.x
2009 Kovalchuk, N., Gilson, J., Pallotta, M., Singh, R., Ismagul, A., Eliby, S. ... Lopato, S. (2009). Characterization of the wheat endosperm transfer cell-specific protein TaPR60. Plant Molecular Biology, 71, 1-2, 81-98. 10.1007/s11103-009-9510-1
2009 Montel, E., Hrmova, M., Fincher, G., Driguez, H. & Cottaz, S. (2009). A chemoenzymatic route to conjugatable beta(1 -> 3)-glucan oligosaccharides. Australian Journal of Chemistry, 62, 6, 575-584. 10.1071/CH08517
2009 Kuntothom, T., Luang, S., Harvey, A., Fincher, G., Opassiri, R., Hrmova, M. & Cairns, J. (2009). Rice family GH1 glycoside hydrolases with β-d-glucosidase and β-d-mannosidase activities. Archives of Biochemistry and Biophysics, 491, 1-2, 85-95. 10.1016/j.abb.2009.09.004
2008 Hrmova, M., Farkas, V., Lahnstein, J. & Fincher, G. B. (2008). A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-β-D-glucans (Journal of Biological Chemistry (2007) 282 (12951-12962)). Journal of Biological Chemistry, 283, 40, 27344-.
2008 Macedo Rodrigues, J., Tucker, M., Johnson, S., Hrmova, M. & Koltunow, A. (2008). Sexual and Apomictic Seed Formation in Hieracium Requires the Plant Polycomb-Group Gene fertilization independent endosperm. Plant Cell, 20, 9, 2372-2386. 10.1105/tpc.108.059287
2007 Hrmova, M., Farkas, V., Lahnstein, J. & Fincher, G. (2007). A barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1 3;1 4)-β-D-glucans. Journal of Biological Chemistry, 282, 17, 12951-12962. 10.1074/jbc.M611487200
2007 Hrmova, M. & Fincher, G. (2007). Dissecting the catalytic mechanism of a plant beta-D-glucan glucohydrolase through structural biology using inhibitors and substrate analogues. Carbohydrate Research, 342, 12-13SI, 1613-1623. 10.1016/j.carres.2007.05.013
2006 Nielsen, K., Hrmova, M., Nielsen, J., Forslund, K., Ebert, S., Olsen, C. ... Moller, B. (2006). Reconstitution of cyanogenesis in barley (Hordeum vulgare L.) and its implications for resistance against the barley powdery mildew fungus. Planta, 223, 5, 1010-1023. 10.1007/s00425-005-0158-z
2006 Burton, R., Wilson, S., Hrmova, M., Harvey, A., Shirley, N., Medhurst, A. ... Fincher, G. (2006). Cellulose synthase-like CslF genes mediate the synthesis of cell wall (1,3;1,4)-beta-D-glucans. Science, 311, 5769, 1940-1942. 10.1126/science.1122975
2006 Farrokhi, N., Burton, R., Brownfield, L., Hrmova, M., Wilson, S., Bacic, A. & Fincher, G. (2006). Plant cell wall biosynthesis: genetic, biochemical and functional genomics approaches to the identification of key genes. Plant Biotechnology Journal, 4, 2, 145-167. 10.1111/j.1467-7652.2005.00169.x
2006 Hrmova, M., Burton, R., Biely, P., Lahnstein, J. & Fincher, G. (2006). Hydrolysis of (1,4)-beta-D-mannans in barley (Hordeum vulgare L.) is mediated by the concerted action of (1,4)-beta-D-mannan endohydrolase and beta-D-mannosidase. Biochemical Journal, 399 Part 1, 1, 77-90. 10.1042/BJ20060170
2006 Zhang, Q., Hrmova, M., Shirley, N., Lahnstein, J. & Fincher, G. (2006). Gene expression patterns and catalytic properties of UDP-D-glucose 4-epimerases from barley (Hordeum vulgare L.). Biochemical Journal, 394, 1, 115-124. 10.1042/BJ20051329
2005 Hrmova, M., Streltsov, V., Smith, B., Vasella, A., Varghese, J. & Fincher, G. (2005). Structural rationale for low-nanomolar binding of transition state mimics to a family GH3 β-D-glucan glucohydrolase from barley. Biochemistry, 44, 50, 16529-16539. 10.1021/bi0514818
2004 Zhang, D., Hrmova, M., Wan, C., Wu, C., Balzen, J., Cai, W. ... Haigler, C. (2004). Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls. Plant Molecular Biology, 54, 3, 353-372. 10.1023/B:PLAN.0000036369.55253.dd
2004 Macdonald, J., Hrmova, M., Fincher, G. & Stick, R. (2004). The synthesis of 3-O-(beta-D-glucopyranosyl)- and 3-O-(beta-laminaribiosyl)-isofagomines, potent inhibitors of a 1,3-beta-D-glucan endo-hydrolase. Australian Journal of Chemistry, 57, 3, 187-191. 10.1071/CH03227
2004 Haigler, C., Zhang, D. & Hrmova, M. (2004). Discovery of genes related to high-rate cellulose synthesis through analysis of gene expression during secondary wall deposition in cotton fibers. Abstracts of Papers of the American Chemical Society, 227, 1, U300-U300.
2004 Strohmeier, M., Hrmova, M., Fischer, M., Harvey, A., Fincher, G. & Pleiss, J. (2004). Molecular modeling of family GH16 glycoside hydrolases: Potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae. Protein Science, 13, 12, 3200-3213. 10.1110/ps.04828404
2004 Hrmova, M., De Gori, R., Smith, B., Vasella, A., Varghese, J. & Fincher, G. (2004). Three-dimensional structure of the barley beta-D-glucan glucohydrolase in complex with a transition state mimic. Journal of Biological Chemistry, 279, 6, 4970-4980. 10.1074/jbc.M307188200
2003 Lee, R., Hrmova, M., Burton, R., Lahnstein, J. & Fincher, G. (2003). Bifunctional family 3 glycoside hydrolases from barley with alpha-L-arabinofuranosidase and beta-D-xylosidase activity - Characterization, primary structures, and COOH-terminal processing. Journal of Biological Chemistry, 278, 7, 5377-5387. 10.1074/jbc.M210627200
2003 Li, J., Burton, R., Harvey, A., Hrmova, M., Wardak, A., Stone, B. & Fincher, G. (2003). Biochemical evidence linking a putative callose synthase gene with (1→3)-β-d-glucan biosynthesis in barley. Plant Molecular Biology, 53, 1, 213-225. 10.1023/B:PLAN.0000009289.50285.52
2003 Fairweather, J., Hrmova, M., Rutten, S., Fincher, G. & Driguez, H. (2003). Synthesis of complex oligosaccharides by using a mutated (1,3)-beta-D-glucan endohydrolase from barley. Chemistry-A European Journal, 9, 11, 2603-2610. 10.1002/chem.200304733
2003 Hrmova, M., Imai, T., Rutten, S., Fairweather, J., Pelosi, L., Bulone, V. ... Fincher, G. (2003). Erratum: Mutated barley (1,3)-β-D-glucan endohydrolases synthesize crystalline (1,3)-β-D-glucans (Journal of Biological Chemistry (2002) 277 (30102-30111)). Journal of Biological Chemistry, 278, 48, 48506-.
2002 Hrmova, M., Imai, T., Rutten, S., Fairweather, J., Pelosi, L., Bulone, V. ... Fincher, G. (2002). Mutated Barley (1,3)-beta-D-Glucan Endohydrolases Synthesize Crystalline (1,3)-beta-D-Glucans. Journal of Biological Chemistry, 277, 33, 30102-30111. 10.1074/jbc.M203971200
2002 Hrmova, M., De Gori, R., Smith, B., Fairweather, J., Driguez, H., Varghese, J. & Fincher, G. (2002). Structural basis for broad substrate specificity in higher plant beta-D-glucan glucohydrolases. Plant Cell, 14, 5, 1033-1052. 10.1105/tpc.010442
2001 Lee, R., Burton, R., Hrmova, M. & Fincher, G. (2001). Barley arabinoxylan arabinofuranohydrolases: purification, characterization and determination of primary structures from cDNA clones. Biochemical Journal, 356, 1, 181-189. 10.1042/bj3560181
2001 Harvey, A., Hrmova, M. & Fincher, G. (2001). Regulation of genes encoding beta-D-glucan glucohydrolases in barley (Hordeum vulgare). Physiologia Plantarum, 113, 1, 108-120. 10.1034/j.1399-3054.2001.1130115.x
2001 Hrmova, M. & Fincher, G. (2001). Structure-function relationships of β- D-glucan endo- and exohydrolases from higher plants. Plant Molecular Biology, 47, 1-2, 73-91. 10.1023/A:1010619128894
2001 Hrmova, M. & Fincher, G. (2001). Plant enzyme structure. Explaining substrate specificity and the evolution of function. Plant Physiology, 125, 1, 54-57. 10.1104/pp.125.1.54
2001 Hrmova, M., Varghese, J., De Gori, R., Smith, B., Driguez, H. & Fincher, G. (2001). Catalytic mechanisms and reaction intermediates along the hydrolytic pathway of a plant beta-D-glucan glucohydrolase. Structure, 9, 11, 1005-1016. 10.1016/S0969-2126(01)00673-6
2001 Osmond, R., Hrmova, M., Fontaine, F., Imberty, A. & Fincher, G. (2001). Binding interactions between barley thaumatin-like proteins and (1,3)-β-D-glucans: Kinetics, specificity, structural analysis and biological implications. FEBS Journal, 268, 15, 4190-4199. 10.1046/j.1432-1327.2001.02331.x
2000 Harvey, A., Hrmova, M., De Gori, R., Varghese, J. & Fincher, G. (2000). Comparative modeling of the three-dimensional structures of family 3 glycoside hydrolases. Proteins-Structure Function and Genetics, 41, 2, 257-269. 10.1002/1097-0134(20001101)41:2<257::AID-PROT100>3.0.CO;2-C
1999 Burton, R., Zhang, X., Hrmova, M. & Fincher, G. (1999). A single limit dextrinase gene is expressed both in the developing endosperm and in germinated grains of barley. Plant Physiology, 119, 3, 859-871. 10.1104/pp.119.3.859
1999 Varghese, J., Hrmova, M. & Fincher, G. (1999). Three-dimensional structure of a barley β-D-glucan exohydrolase, a family 3 glycosyl hydrolase. Structure, 7, 2, 179-190. 10.1016/S0969-2126(99)80024-0
1998 Hrmova, M., MacGregor, E., Biely, P., Stewart, R. & Fincher, G. (1998). Substrate binding and catalytic mechanism of a barley b-D-glucosidase (1,4)-b-D-glucan exohydrolase. Journal of Biological Chemistry, 273, 18, 11134-11143. 10.1074/jbc.273.18.11134
1998 Hrmova, M. & Fincher, G. (1998). Barley b-D-glucan exohydrolases. Substrate specificity and kinetic properties. Carbohydrate Research, 305, 2, 209-221. 10.1016/S0008-6215(97)00257-7
1998 Hrmova, M., Varghese, J., Hoj, P. & Fincher, G. (1998). Crystallization and preliminary X-ray analysis of β-glucan exohydrolase isoenzyme Exol from barley (Hordeum vulgare). Acta Crystallographica Section D, Biological Crystallography, D54, 4, 687-689. 10.1107/S0907444997018866
1998 Hrmova, M., Fincher, G., Viladot, J. L., Planas, A. & Driguez, H. (1998). Chemoenzymic synthesis of (1→3,1→4)-β-D-glucooligosaccharides for subsite mapping of (1→3,1→4)-β-D-glucan endohydrolases. Journal of Chemical Society Perkin Transactions 1, 21, 21, 3571-3576. 10.1039/A804711A
1997 Hrmova, M., Banik, M., Harvey, A., Garrett, T., Varghese, J., Hoj, P. & Fincher, G. (1997). Polysaccharide hydrolases in germinated barley and their role in the depolymerization of plant and fungal cell walls. International Journal of Biological Macromolecules, 21, 1-2, 67-72. 10.1016/S0141-8130(97)00043-3
1997 Akiyama, T., Shibuya, N., Hrmova, M. & Fincher, G. (1997). Purification and characterization of a (1-3)-B-D-glucan endohydrolase from rice (Oryza sativa) bran. Carbohydrate Research, 297, 4, 365-374. 10.1016/S0008-6215(96)00291-1
1996 Hrmova, M., Harvey, A., Wang, J., Shirley, N., Jones, G., Stone, B. ... Fincher, G. (1996). Barley β-D-glucan exohydrolases with β-D-glucosidase activity. Journal of Biological Chemistry, 271, 9, 5277-5286. 10.1074/jbc.271.9.5277
1996 Hrmova, M., Harvey, A., Wang, J., Shirley, N., Jones, G., Stone, B. ... Fincher, G. (1996). Barley β-D-glucan exohydrolases with β-D-glucosidase activity: Purification, characterization, and determination of primary structure from a cDNA clone. Journal of Biological Chemistry, 271, 9, 5277-5286. 10.1074/jbc.271.9.5277
1995 Hrmova, M., Garrett, T. & Fincher, G. (1995). Subsite affinities and disposition of catalytic amino acids in the substrate-binding region of barley 1,3-β-glucanases. Implications in plant-pathogen interactions. Journal of Biological Chemistry, 270, 24, 14556-14563. 10.1074/jbc.270.24.14556
1993 Hrmova, M. & Fincher, G. (1993). Purification and properties of three (1→3)-β-D-glucanase isoenzymes from young leaves of barley (Hordeum vulgare). Biochemical Journal, 289, 2, 453-461. 10.1042/bj2890453

Book Chapters

Year Citation
2017 Luang, S. & Hrmova, M. (2017). Structural basis of the permeation function of plant aquaporins. In F. Chaumont & S. Tyerman (Eds.), Plant Aquaporins: From Transport to Signalling (pp. 1-28). Berlin, Heidelberg, Lon: Springer. 10.1007/978-3-319-49395-4_1
2014 Hrmova, M. & Lopato, S. (2014). Enhancing abiotic stress tolerance in plants by modulating properties of stress responsive transcription factors. In R. Tuberosa, A. Graner & E. Frison (Eds.), Genomics of plant genetic resources. Volume 2. Crop productivity, food security and nutritional quality (pp. 291-316). Springer Netherlands. 10.1007/978-94-007-7575-6_12
2011 Stone, B., Jacobs, A., Hrmova, M., Burton, R. & Fincher, G. (2011). Biosynthesis of plant cell wall and related polysaccharides by enzymes of the GT2 and GT48 families. In Peter Ulvskov (Ed.), Annual Plant Reviews, Volume 41, Plant Polysaccharides: Biosynthesis and Bioengineering (pp. 109-165). United Kingdom: Wiley. 10.1002/9781444391015.ch5
2009 Hrmova, M. & Fincher, G. (2009). Plant and microbial enzymes involved in the depolymerization of (1,3)-β-d-glucans and related polysaccharides. In A. Bacic, G. B. Fincher & B. A. Stone (Eds.), Chemisty, Biochemistry, and Biology of the (1,3)-B-D-Glucans and related Polysaccharides (pp. 119-170). USA: Academic Press. 10.1016/B978-0-12-373971-1.00004-2
2009 Hrmova, M. & Fincher, G. (2009). Functional genomics and structural biology in the definition of gene function. In D. Somers, P. Langridge & J. Gustafson (Eds.), Plant genomics: Methods and Protocols (pp. 199-227). United States: Humana Press. 10.1007/978-1-59745-427-8_11
2009 Farrokhi, N., Hrmova, M., Burton, R. & Fincher, G. (2009). Heterologous and cell-free protein expression systems. In D. Somers, J. Gustafson & P. Langridge (Eds.), Plant genomics: Methods and Protocols (pp. 175-198). United States: Humana Press. 10.1007/978-1-59745-427-8_10
2003 Hrmova, M. & Fincher, G. (2003). Enzymic Hydrolysis of Cereal (1-3, 1-4)-beta-Glucans. In Handbook of Food Enzymology (pp. 943-960). New York: Marcel Dekker Inc.
2001 Hrmova, M. & Fincher, G. (2001). Structure function relationships of β-D-glucan endo- and exohydrolases from higher plants. In N. Carpita, M. Campbell & M. Tierney (Eds.), Plant cell walls (pp. 73-91). Dodrecht ; Boston: Kluwer Academic Publishers. 10.1007/978-94-010-0668-2_5

Conference Papers

Year Citation
2007 Hrmova, M., Farkas, V., Harvey, A. & Fincher, G. (2007). Structure-function studies of xyloglucan transglycosylases/hydrolases. International Fungal and Plant Cell Wall Meeting. Biarritz, France.
2005 Eariss, G., Hrmova, M., Fincher, G. & Catcheside, D. (2005). Diversification of barley beta-D-glucan endohydrolases in Neurospora crassa..
2000 Harvey, A., Hrmova, M. & Fincher, G. (2000). Regulation of Genes Encoding b-Glucan Exohydrolases in Barley (Hordeum vulgare L.). 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.
2000 Hrmova, M., Rutten, S., Fincher, G. & Driguez, H. (2000). Wild Type and Mutant (13)-b-Glucan Endohyrolases From Barley are Efficient Tools for Synthesis (13)-b Linked Oligo- and Polysaccharides. 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.
2000 Harvey, A., Hrmova, M., De Gori, R., Varghese, J. & Fincher, G. (2000). Comparative Modeling of the Three-Dimensional Structures and Phylogenetic Analysis of Family 3 Glycoside Hydrolases. 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.
2000 Hrmova, M., Varghese, J., Harvey, A., Stewart, R. & Fincher, G. (2000). Enzymic Hydrolysis of Barley (13,14)-b-Glucans. 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.
2000 Hrmova, M., Harvey, A., Lee, R., Banik, M., Varghese, J. & Fincher, G. (2000). Cell wall degrading enzymes in barley.
2000 Rutten, S., Hrmova, M., Burton, R. & Fincher, G. (2000). Altering the Substrate Specificity of Barley (13)-b-Glucan Endohydrolase Isoenzyme GII. 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.
2000 Lee, R., Burton, R., Hrmova, M. & Fincher, G. (2000). a-L-Arabinofuranosidases and a b-D-Xylosidases From Germinated Barley. 8th International Barley Genetics Symposium. D. S. J. Logue (Ed.) Adelaide, Australia.

Curated or Produced Public Exhibition or Events

Year Citation
2015 Waters, S., Gilliham, M. & Hrmova, M.; (2015); Micro-biomachines helping plants survive salt; University of Adelaide; E-Science Magazine of the University of Adelaide;
2011 Hrmova, M.; (2011); Two centenaries; 13th issue of the ACPFG Vector magazine.;
2010 Hrmova, M.; (2010); The Bragg crystallography facility is up and running; Adelaidean; October issue, News from the University of Adelaide (UoA);
2010 Hrmova, M.; (2010); A new ‘Protein Structure/Function Focus Group’ at the ACPFG is up and running'; 11th issue of the ACPFG Vector magazine;
2009 Hrmova, M.; (2009); Small but significant. Adelaide’s Advertiser, section ‘Education Now’; Adelaide’s Advertiser, section ‘Education Now’;
2009 Hrmova, M.; (2009); Unravelling protein structures through molecular modelling; 9th issue of the ACPFG Vector magazine;
2007 Hrmova, M.; (2007); The beauty of discovery is in simplicity; Newspaper ‘Pravda’ in Bratislava (capital of Slovakia);
2006 Hrmova, M.; (2006); Hrmova M (2006) X-Ray crystallography gazes into the unknown world of proteins; Spring issue of the ACPFG Vector magazine;
2006 Hrmova, M.; (2006); Hrmova M (2006) Crystal gazing. Adelaide’s Saturday Advertiser, section Can you believe it?; Saturday Advertiser, section Can you believe it?;

Datasets

Year Citation
2016 Baumann, U., hrmova, M., harris, J., lopato, S., langridge, P., Sornaraj, P. & lovell, B.; (2016); Molecular interactions of the gamma-clade homeodomain-leucine zipper class I transcription factors during the wheat response to water deficit;

Research in the Hrmova group is funded by grants from the Australian Research Council, by the Grains Research & Development Corporation, the South Australian Government, the Waite Research Institute of the University of Adelaide, DuPont Pioneer and by the Australian Synchrotron Research Program. The latter is supported by the Commonwealth of Australia under the Major National Research Facilities Program.

I have successfully completed supervisions of 25 doctoral (PhD), masters (MSc) and honours (BSc) candidatures by research. My students have received a variety of awards: Best Presentation in Plant Science and runner up for the Max Tate Award (in-house Annual Postgraduate Symposium), People’s Choice (3-minute PhD thesis competition), Travelling Scholarship to Cambridge University (Barr Smith Foundation), the KP Barley Prize for PhD thesis excellence (Faculty of Sciences), and CJ Everald and GRDC top-up scholarships.

I have been involved in guidance of academic staff and supervision of post-doctoral and other research personnel at all levels within the University of Adelaide, as well as of research staff arriving from various international universities and institutions. My role has been to ensure that the research goals specified in the grants funded by the ongoing ARC, Grains Research and Development Corporation and CSIRO grant applications were fulfilled on time. This advisory role included guidance of researchers in a range of computational and biochemical projects at the Waite Campus, such as those from The CSIRO Division of Plant Industry, The Australian Grain Technologies, The Australian Wine Research Institute and The Australian Genome Research Facility. To illustrate my activity, I generated a 3D model of S-RNases; this model helped to explain almond self-incompatibility, and initiated novel approaches in this research. I have also served as an advisor to the research groups at ACPFG since the centre was established in 2003, with its 120 working staff. Here my role has been to oversee computational and biochemical aspects of all projects.

Position
Professor
Phone
83137160
Fax
8313 7102
Campus
Waite
Building
Plant Genomics Centre
Room Number
2 36
Org Unit
Australian Centre for Plant Functional Genomics (ACPFG)

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