Keith Shearwin

Associate Professor Keith Shearwin

Associate Professor

School of Biological Sciences

Faculty of Sciences

Eligible to supervise Masters and PhD - email supervisor to discuss availability.


Overview

Our research integrates biochemistry, genetics and mathematical modelling to characterise fundamental mechanisms of gene control and how these elements are combined to create gene regulatory circuits with complex functions. Having a detailed understanding of the properties of natural regulatory circuits makes it possible to rearrange these components to build circuits with new and interesting behaviours - the emerging discipline of Synthetic Biology. We have a strong focus on using and developing mathematical modelling as a tool to advance understanding of gene regulatory mechanisms and systems, a tool that will be essential for the new ‘systems biology’ as molecular biology tries to move from characterizing the parts of cells and their simple interactions to understanding how these interactions combine to generate complex functions.  We find that the process of attempting to construct a mathematical model of a biological system is valuable in itself - helping to clarify ideas, define assumptions and identify missing information. Once a model is made, comparison of the model with available experimental data can reveal shortcomings in either, leading to changes to the model or to improved data collection or interpretation. A consistent mathematical description is a powerful tool, providing precise predictions to aid further experimental tests, and allowing rapid exploration of the range of properties of the system to generate insights into design features.

Our primary experimental systems are two E. coli bacteriophages, lambda and 186. These temperate phages can replicate their genomes using alternative developmental pathways, lysis and lysogeny, and are some of the simplest organisms to make developmental decisions. Despite their relative simplicity, the phage systems combine a wide range of gene control mechanisms in complex ways and have many lessons to teach us. Bacteriophage lambda continues to be a key model system for many molecular biological processes; phage 186 is less well characterised but provides a powerful comparison with lambda, as it achieves similar outcomes using different regulatory circuits. The fundamental biochemistry shared by all living things means that the study of any organism, from phages to humans, continues to illuminate universal principles that apply to all organisms.

 

Current Funding

Synthetic biology tools for integration into bacterial chromosomes. ARC Discovery Grant DP160101450 (2016-2018) Shearwin, K.E. and Bikard, D. $410 000

Understanding and manipulating DNA looping in long range gene regulation. NHMRC Project Grant APP1100651 (2016-2018) Dodd, I.B., Shearwin, K.E., Sneppen, K. and Hao, N. $351 000

Flipping the switch: rational design of genetic circuits that respond to transient signals. ARC Discovery Grant DP150103009  (2015-2017) Shearwin, K.E., Dodd, I.B. and Sneppen, K. $241 000

Previous Funding

Quantitative analysis of the DNA loop-domain model for long range regulation of transcription. Human Frontiers Science Program  RGP0051 (2009-2012) Shearwin, K.E., Dunlap, D. and Swigon, D. $1 251 000 (total)

Road rules for traffic on DNA - gene regulation by encounters between transcribing RNA polymerases and DNA-bound proteins. ARC Discovery Grant DP110101470  (2011-2013) Dodd, I.B., Shearwin, K.E., and Sneppen, K. $300 000

Synthetic Biology - The Rational Design and Construction of New Genetic Circuits. ARC Discovery Grant DP110100824 (2011-2013) Shearwin, K.E., Dodd, I.B., Finzi, L. and Sneppen, K. $300 000

Understanding and controlling PAS domain interactions in basic helix-loop-helix transcription factors. NHMRC APP627140 (2010-2012) Chapman-Smith, A., Whitelaw, M.L., Shearwin, K.E. and Dodd, I.B. $608 000

Rational design of small genetic circuits for synthetic biology. ARC Discovery Grant DP0665185 (2006-2008) Shearwin, K.E. and Egan, J.B. $368 000

Transcriptional Interference in Gene Regulatory Decisions. NIH R01 (2006-2009) Egan, J.B., Dodd, I.B. and Shearwin, K.E. $526 000

The South Australian Facility for Small and Large Molecule X-Ray Diffraction Structure Determination. ARC LIEF(2009) Sumby, C.J, Shearwin, K.E. and 26 others $560 000

Biophysical Characterisation Facility. ARC LIEF Grant LE0882382 (2008) Brooks, D.A., Shearwin, K.E. (lead CI for University of Adelaide) and 14 others $245 000

Biophysical Characterisation Facility. AIB Labs (2008) Brooks, D.A., Shearwin, K.E. (lead CI for University of Adelaide) and 14 others $50 000

ARC Australian Research Fellowship (1996-2000) Shearwin, K.E. $400 000

 

 

In 2018 I am teaching in the following courses

Fundamentals of Biomedical Science (lectures)

Biochemistry II (lectures)

Biomedical Science IIA (lectures)

Biotechnology II (lectures)

Principles and Practice of Research II (mentor, PBLs)

Principles and Practice of Research III (mentor, research placements)

Molecular and Structural Biology III (lectures, tutorials, practicals)

Cancer, Stem Cells and Development (Biomed) (PBL)

Cancer, Stem Cells and Development III (essays)

Masters in Biotechnology: Advanced Research Platforms (lectures)

Honours in Biochemistry (journal club; research grant proposal and thesis assessment)

Laboratory Placements

We host 3rd year laboratory placement students each year.

2018: Smriti Singh, Grace Tulysewski

2017: Khalia Primer

2016: Emi Schutz, Daniel Reed

2015: Jasmine Kelly, Mara Zeissig, Alejandra Isabel, Joe Rossi, Matilda Handsley-Davis

2014: Dominique Jackson, Queenie Tangseefa, Jade Feong, Andrew Hayes, Trang Pham

2013: Byron Shue, Jia Truong, Andelain Erickson

2012: Duncan McKenzie, Dylan Agnew, Grace De La Lande

2011: Emma Rollo, Ashleigh Paparella, Katrina Lee

2010: Emily Higginson, Sofia Xuan, George Dimitroff

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  • Current Higher Degree by Research Supervision (University of Adelaide)

    Date Role Research Topic Program Degree Type Student Load Student Name
    2019 Principal Supervisor Bacteriophage based applications in synthetic biology Doctor of Philosophy Doctorate Full Time Lawrence Daniel Beltrame
    2019 Co-Supervisor Protein Engineering of P450 Enzymes into Functional Peroxygenases Doctor of Philosophy Doctorate Full Time Mr Joel Hoong Zhang Lee
    2018 Co-Supervisor Biochemistry Doctor of Philosophy Doctorate Full Time Ms Stephanie Nguyen
    2018 Co-Supervisor Biochemistry Doctor of Philosophy Doctorate Full Time Mr Jordan Luke Pederick
    2018 Principal Supervisor Phage based antibacterial proteins Doctor of Philosophy Doctorate Full Time Miss Hannah Rose Bonham
    2017 Principal Supervisor Catalytically Inactive Cas Proteins as Transcriptional Roadblocks to Modulate Gene Expression Doctor of Philosophy Doctorate Full Time Miss Alana Jane Donnelly
    2017 Principal Supervisor Investigate the Role of 186 Bacteriophage Regulatory Proteins CI and Apl in Prophage Induction Doctor of Philosophy Doctorate Full Time Miss Alejandra Isabel
    2016 Co-Supervisor Mas Spectrometric Analysis of Higher Order DNA and Protein Structures Doctor of Philosophy Doctorate Full Time Mr Jiawei Li
  • Past Higher Degree by Research Supervision (University of Adelaide)

    Date Role Research Topic Program Degree Type Student Load Student Name
    2011 - 2014 Principal Supervisor Testing the DNA loop domain model in Escherichia coli Doctor of Philosophy Doctorate Full Time Mr David Priest
    2010 - 2014 Principal Supervisor DNA looping mediated transcriptional regulation Doctor of Philosophy Doctorate Full Time Mr Lun Cui
    2008 - 2015 Principal Supervisor The Design, Synthesis and Quantitative Analysis of a Bistable Mixed Feedback Loop Gene Network Doctor of Philosophy Doctorate Full Time Mr Julian Pietsch
    2006 - 2008 Co-Supervisor Calcium and Phosphoinositides Regulations in Alpha-Actinin-4 F-Actin Binding Doctor of Philosophy Doctorate Full Time Mr Huang-Hui Chen
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  • Committee Memberships

    Date Role Committee Institution Country
    2017 - ongoing Member Postgraduate Coordinator The University of Adelaide Australia
    2015 - ongoing Chair School of Biological Sciences Research Education Committee
  • Memberships

    Date Role Membership Country
    2015 - ongoing Representative Synthetic Biology Australasia Australia
    1993 - ongoing Member Australian Society for Biochemistry and Molecular Biology Australia
  • Position: Associate Professor
  • Phone: 83135361
  • Email: keith.shearwin@adelaide.edu.au
  • Fax: 8313 4362
  • Campus: North Terrace
  • Building: Molecular Life Sciences, floor 2
  • Room: 2 41
  • Org Unit: Molecular and Biomedical Science

Connect With Me
External Profiles