Dr Cynthia Bottema
Associate Professor
School of Animal and Veterinary Science
Faculty of Sciences, Engineering and Technology
Eligible to supervise Masters and PhD - email supervisor to discuss availability.
Dr Cynthia Bottema is an Associate Professor at the University of Adelaide in the School of Animal & Veterinary Sciences. Her expertise is in molecular genetics, DNA testing, biotechnology, and lipid biochemistry, particularly as applied to animal science and livestock production. Cindy received her PhD in Microbial Physiology at Oregon State University. Upon completion of a post-doctoral fellowship at Stanford University, Cindy was appointed as a human molecular genetics research associate at the Mayo Clinic. In 1992, Cindy took a position at the University of Adelaide as the JS Davies Professorial Research Fellow in cattle molecular genetics and in 2000, became a tenured academic at the University of Adelaide. Since 2000, Cindy has had several educational roles in the University including acting as the Animal Science Honours Coordinator, Animal Science, Program Coordinator, the School Associate Head of Staff, the Head of the Animal & Veterinary Biosciences Department and a member of the School Executive Committee.
Cindy’s teaching interests include genetics, molecular genetics, biotechnology, biochemistry, and microbiology. Her research interests are in livestock molecular genetics and include the genome organization of Bovidae, genome mapping in Bovidae and genetics of lipid metabolism in cattle. Cindy served on the Executive Committee of the International Society of Animal Genetics for many years.
PREVIOUS RESEARCH INTERESTS
Sterol metabolism in yeast. Projects included:
- Characterisation of the enzyme 14D-sterol reductase in the ergosterol biosynthetic pathway of fungi.
- Quantitation and analysis of sterols in yeast mitochondria and plasma membranes.
- Thermotropic and enzymatic studies on yeast membranes with altered sterol compositions.
Steroid binding in yeast. Projects included:
- Characterisation and purification of an estrogen binding protein in the yeast Saccharomyces cerevisiae.
- Isolation and characterisation of ligands of the yeast estrogen binding protein.
- Comparative studies of mammalian estrogen receptors from tissue culture and rats.
Molecular genetics of human disease. Projects included:
- Delineation and characterisation of mutations in the factor IX gene of haemophilia B patients.
- Analysis of the pattern of germline mutations, mutation rates and evolutionary conservation in humans by population-based molecular genetic studies.
- Development of PCR and sequencing techniques for DNA and RNA diagnostics, RFLP analysis and direct carrier testing.
Genome organisation of Bovidae. Projects included:
- Isolation and characterisation of centromeric and interspersed repetitive DNA sequences from cattle and sheep.
- Characterisation of Robertsonian translocations in cattle by in situ hybridisation using centromeric probes.
- Development of comparative physical gene maps for cattle, sheep, goat and deer by fluorescence in situ hybridisation (FISH) using ovine bacterial artificial chromosome (BAC) clones.
- Development of low cost methodologies for parentage testing in cattle and sheep.
CURRENT RESEARCH INTERESTS
Genome mapping in Bovidae. Projects include:
- Mapping quantitative trait loci (QTL) in cattle for meat quality, particularly fat and muscle metabolic traits.
- Isolation and characterisation of genes involved in ruminant lipid metabolism and deposition.
- Isolation and characterisation of genes involved in cattle net feed efficiency.
- Development of molecular marker techniques for livestock applications.
Genetics of lipid metabolism in cattle. Projects include:
- Analysis of the fatty acid composition in the phospholipids and triacylglycerols of muscle and adipose tissues in different cattle breeds.
- Delineation of carotenoid uptake and deposition in bovine adipose tissue.
- Elucidation of genetics controlling intramuscular fat variation in cattle breeds.
- Determination of effects of vitamin A on adipogenesis in Bovidae.
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Appointments
Date Position Institution name 1992 - ongoing Associate Professor University of Adelaide -
Education
Date Institution name Country Title University of Colorado USA BA (major: Molecular, Cellular & Developmental Biology) Oregon State University USA PhD -
Research Interests
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Journals
Year Citation 2022 Tamlin, V. S., Bottema, C. D. K., Woolford, L., Dobson, E. C., Kessell, A. E., & Peaston, A. E. (2022). Canine mast cell tumours part I: Clinical and survival outcomes. Veterinary Medicine and Science, 8(4), 1409-1420.
2021 Tamlin, V. S., Bottema, C. D. K., Campbell-Ward, M. L., Hanshaw, D., & Peaston, A. E. (2021). KIT mutations in mast cell tumours from cheetahs (Acinonyx jubatus) and domestic cats (Felis catus).. Vet Comp Oncol, 19(2), 381-392.
Scopus1 WoS1 Europe PMC12021 Hue, D. T., Skirving, R., Chen, T., Williams, J. L., Bottema, C. D. K., & Petrovski, K. (2021). Colostrum source and passive immunity transfer in dairy bull calves. Journal of Dairy Science, 104(7), 8164-8176.
Scopus2 WoS12021 Hue, D. T., Williams, J. L., Petrovski, K., & Bottema, C. D. K. (2021). Predicting colostrum and calf blood components based on refractometry. Journal of Dairy Research, 88(2), 194-200.
Scopus2 WoS2 Europe PMC12020 Hiendleder, S., Bottema, C. D. K., & Brunner, R. M. (2020). Nuclear mitochondrial pseudogenes in the cattle genome. Animal Genetics, 51(1), 148-149.
Scopus1 WoS22020 Tamlin, V. S., Bottema, C. D. K., & Peaston, A. E. (2020). Comparative aspects of mast cell neoplasia in animals and the role of KIT in prognosis and treatment.. Veterinary medicine and science, 6(1), 3-18.
Scopus11 WoS8 Europe PMC42020 Bottema, M. J., Kruk, Z. A., Gontar, A., Pitchford, W. S., & Bottema, C. D. K. (2020). Evidence of marbling as a single connected entity in beef striploins.. Meat Sci, 161, 108004.
Scopus6 WoS52020 Aldersey, J. E., Sonstegard, T. S., Williams, J. L., & Bottema, C. D. K. (2020). Understanding the effects of the bovine POLLED variants. Animal Genetics, 51(2), 166-176.
Scopus8 WoS8 Europe PMC42018 Kruk, Z., Bottema, M., Reyes-Veliz, L., Forder, R., Pitchford, W., & Bottema, C. (2018). Vitamin A and marbling attributes: intramuscular fat hyperplasia effects in cattle. Meat Science, 137, 139-146.
Scopus17 WoS16 Europe PMC22018 Herd, R., Arthur, P., Bottema, C., Egarr, A., Geesink, G., Lines, D., . . . Pitchford, W. (2018). Genetic divergence in residual feed intake affects growth, feed efficiency, carcass and meat quality characteristics of Angus steers in a large commercial feedlot. Animal Production Science, 58(1), 164-174.
Scopus11 WoS112018 Lines, D., Pitchford, W., Bottema, C., Herd, R., & Oddy, V. (2018). Selection for residual feed intake affects appetite and body composition rather than energetic efficiency. Animal Production Science, 58(1), 175-184.
Scopus30 WoS292015 FAANG Consortium., Andersson, L., Archibald, A., Bottema, C., Brauning, R., Burgess, S., . . . Zhou, H. (2015). Coordinated international action to accelerate genome-to-phenome with FAANG, the Functional Annotation of Animal Genomes project. Genome Biology, 16(1), 57-1-57-6.
Scopus182 WoS183 Europe PMC1412015 Rosenblatt, A., Hill, P., Davies, S., Webster, N., Lappalainen, A., Bottema, C., & Caraguel, C. (2015). Precision of spinal radiographs as a screening test for intervertebral disc calcification in Dachshunds. Preventive Veterinary Medicine, 122(1-2), 164-173.
Scopus3 WoS3 Europe PMC22014 Rosenblatt, A., Bottema, C., & Hill, P. (2014). Radiographic scoring for intervertebral disc calcification in the Dachshund. The Veterinary Journal, 200(3), 355-361.
Scopus4 WoS4 Europe PMC32014 Chang, L., Pitchford, W., & Bottema, C. (2014). Modeling tenderness for genetic and quantitative trait loci analyses. Journal of Animal Science, 92(1), 39-47.
Scopus2 WoS22013 Morris, C., Bottema, C., Cullen, N., Hickey, S., Knowles, S., & Pitchford, W. (2013). Effects of quantitative trait loci and the myostatin locus on trace and macro elements (minerals) in bovine liver, muscle and kidney. Animal Genetics, 44(4), 361-368.
Scopus7 WoS7 Europe PMC62012 Tian, R., Cullen, N., Morris, C., Fisher, P., Pitchford, W., & Bottema, C. (2012). Major effect of retinal short-chain dehydrogenase reductase (RDHE2) on bovine fat colour. Mammalian Genome, 23(5-6), 378-386.
Scopus7 WoS6 Europe PMC32011 Esmailizadeh Koshkoih, A., Morris, C., Cullen, N., Kruk, Z., Lines, D., Hickey, S., . . . Pitchford, W. (2011). Genetic mapping of quantitative trait loci for meat quality and muscle metabolic traits in cattle. Animal Genetics, 42(6), 592-599.
Scopus11 WoS10 Europe PMC82011 Abdolmohammadi, A., Atashi, H., Zamani, P., & Bottema, C. (2011). High resolution melting as an alternative method to genotype diacylglycerol O-acyltransferase 1 (DGAT1) K232A polymorphism in cattle. Czech Journal of Animal Science, 56(8), 370-376.
Scopus11 WoS112010 Morris, C., Bottema, C., Cullen, N., Hickey, S., Esmailizadeh, A., Siebert, B., & Pitchford, W. (2010). Quantitative trait loci for organ weights and adipose fat composition in Jersey and Limousin back-cross cattle finished on pasture or feedlot. Animal Genetics, 41(6), 589-596.
Scopus23 WoS26 Europe PMC202010 Pisani, D., Bottema, C., Butori, C., Dani, C., & Dechesne, C. (2010). Mouse model of skeletal muscle adiposity: A glycerol treatment approach. Biochemical and Biophysical Research Communications, 396(3), 767-773.
Scopus44 WoS42 Europe PMC252010 Tian, R., Pitchford, W., Morris, C., Cullen, N., & Bottema, C. (2010). Genetic variation in the β, β-carotene-9′, 10′-dioxygenase gene and association with fat colour in bovine adipose tissue and milk. Animal Genetics, 41(3), 253-259.
Scopus52 WoS49 Europe PMC372009 Morris, C., Pitchford, W., Cullen, N., Esmailizadeh Koshkoih, A., Hickey, S., Hyndman, D., . . . Bottema, C. (2009). Quantitative trait loci for live animal and carcass composition traits in Jersey and Limousin back-cross cattle finished on pasture or feedlot. Animal Genetics, 40(5), 648-654.
Scopus19 WoS21 Europe PMC142009 Lines, D., Pitchford, W., Kruk, Z., & Bottema, C. (2009). Limousin myostatin F94L variant affects semitendinosus tenderness. Meat Science, 81(1), 126-131.
Scopus15 WoS14 Europe PMC12008 Esmailizadeh, A., Bottema, C., Sellick, G., Verbyla, A., Morris, C., Cullen, N., & Pitchford, W. (2008). Effects of the myostatin F94L substitution on beef traits. Journal of Animal Science, 86(5), 1038-1046.
Scopus64 WoS65 Europe PMC382008 Kurk, Z., Bottema, C., Davis, J., Siebert, B., Harper, G., Di, J., & Pitchford, W. (2008). Effects of vitamin A on growth performance and carcass quality in steers. Livestock Science, 119(1-3), 12-21.
Scopus30 WoS242007 Sellick, G., Pitchford, W., Morris, C., Cullen, N., Crawford, A., Raadsma, H., & Bottema, C. (2007). Effect of myostatin F94L on carcass yield in cattle. Animal Genetics, 38(5), 440-446.
Scopus47 WoS49 Europe PMC272007 Morris, C., Cullen, N., Glass, B., Hyndman, D., Manley, T., Hickey, S., . . . Lee, M. (2007). Fatty acid synthase effects on bovine adipose fat and milk fat. Mammalian Genome, 18(1), 64-74.
Scopus115 WoS114 Europe PMC702006 Reverter, A., Hudson, N., Wang, Y., Tan, S., Barris, W., Byrne, K., . . . Dalrymple, B. (2006). A gene coexpression network for bovine skeletal muscle inferred from microarray data. Physiological Genomics, 28(1), 76-83.
Scopus28 WoS26 Europe PMC212006 Morris, C., Cullen, N., Hickey, S., Dobbie, P., Veenvliet, B., Manley, T., . . . Wilson, T. (2006). Genotypic effects of calpain 1 and calpastatin on the tenderness of cooked M. longissimus dorsi steaks from Jersey x Limousin, Angus and Hereford-cross cattle. Animal Genetics, 37(4), 411-414.
Scopus61 WoS62 Europe PMC282006 Siebert, B., Kruk, Z., Davis, J., Pitchford, W., Harper, G., & Bottema, C. (2006). Effect of low vitamin a status on fat deposition and fatty acid desaturation in beef cattle. Lipids, 41(4), 365-370.
Scopus35 WoS31 Europe PMC152006 Beever, J., Smit, M., Meyers, S., Hadfield, T., Bottema, C., Albretsen, J., & Cockett, N. (2006). A single-base change in the tyrosine kinase II domain of ovine FGFR3 causes hereditary chondrodysplasia in sheep. Animal Genetics, 37(1), 66-71.
Scopus49 WoS42 Europe PMC312005 Kruk, Z., & Bottema, C. (2005). The guts of the matter: dietary regulation of intestinal gene expression. Recent Advances in Animal Nutrition in Australia, 15, 87-93. 2005 Yu, S., Kim, J., Chung, H., Jung, K., Lee, Y., Yoon, D., . . . Lee, J. (2005). Molecular cloning and characterization of bovine PRKAG3 gene: structure, expression and single nucleotide polymorphism detection. Journal of Animal Breeding and Genetics-zeitschrift fur Tierzuchtung und Zuchtungsbiologie, 122(5), 294-301.
Scopus13 WoS11 Europe PMC22004 Kuchel, H., Siebert, B., Bottema, C., Webb, G., Crawford, A., Duncan, S., . . . Pitchford, W. (2004). Physical mapping of the stearoyl-CoA desaturase (SCD) locus in sheep. Animal Genetics, 35(2), 163.
Scopus6 WoS6 Europe PMC22004 Hebart, M., Pitchford, W., Arthur, P., Archer, J., Herd, R., & Bottema, C. (2004). Effect of missing data on the estimate of average daily feed intake in beef cattle. Australian Journal of Experimental Agriculture, 44(4-5), 415-421.
Scopus11 WoS102003 Siebert, B., Pitchford, W., Kruk, Z., Kuchel, H., Deland, M., & Bottema, C. (2003). Differences in Δ⁹ desaturase activity between Jersey- and Limousin-sired cattle. Lipids, 38(5), 539-543.
Scopus38 WoS35 Europe PMC242003 Pal, S., Thomson, A., Bottema, C., & Roach, P. (2003). α-Tocopherol modulates the low density lipoprotein receptor of human HepG2 cells-Tocopherol modulates the low density lipoprotein receptor of human HepG2 cells. Nutrition Journal, 2(1), 3-13.
Scopus19 WoS22 Europe PMC102002 Afolayan, R., Deland, M., Rutley, D., Bottema, C., Ewers, A., Ponzoni, R., & Pitchford, W. (2002). Prediction of carcass meat, fat and bone yield across diverse cattle genotypes using live-animal measurements. Animal Production, 24, 13-16. 2002 Kurar, E., Barendse, W., Bottema, C., Davis, S., Foster, M., Kalm, E., . . . Kirkpatrick, B. (2002). Concensus and comprehensive linkage maps of bovine chromosome 24. Animal Genetics, 33(6), 460-463.
Scopus5 WoS4 Europe PMC32002 Afolayan, R., Pitchford, W., Weatherly, A., & Bottema, C. (2002). Genetic variation in growth and body dimensions of Jersey and Limousin cross cattle. 2. Post-weaning dry and wet season performance. Asian-Australasian Journal of Animal Sciences, 15(10), 1378-1385.
Scopus5 WoS32002 Afolayan, R., Pitchford, W., Weatherly, A., & Bottema, C. (2002). Genetic variation in growth and body dimensions of Jersey and Limousin cross cattle. 1. Pre-weaning performance. Asian-Australasian Journal of Animal Sciences, 15(10), 1371-1377.
Scopus7 WoS52002 Pitchford, W., Deland, M., Siebert, B., Malau-Aduli, A., & Bottema, C. (2002). Genetic variation in fatness and fatty acid composition of crossbred cattle. Journal of Animal Science, 80(11), 2825-2832.
Scopus113 WoS109 Europe PMC732002 Kruk, Z., Pitchford, W., Siebert, B., Deland, M., & Bottema, C. (2002). Factors affecting estimation of marbling in cattle and the relationship between marbling scores and intramuscular fat. Animal Production, 24, 129-132. 2002 Pal, S., Thomson, A., Bottema, C., & Roach, P. (2002). Polyunsaturated fatty acids downregulate the low density lipoprotein receptor of human HepG2 cells. Journal of Nutritional Biochemistry, 13(1), 55-63.
Scopus15 WoS14 Europe PMC132001 Wheeler, D., Hope, R., Cooper, S., Dolman, G., Webb, G., Bottema, C., . . . Holland, R. (2001). An orphaned mammalian b-globin gene of ancient evolutionary origin. Proceedings of the National Academy of Sciences of the United States of America, 98(3), 1101-1106.
Scopus43 WoS43 Europe PMC282001 Bursill, C., Roach, P., Bottema, C., & Pal, S. (2001). Green tea upregulates the low-density lipoprotein receptor through the sterol-regulated element binding protein in HepG2 liver cells. Journal of Agricultural and Food Chemistry, 49(11), 5639-5645.
Scopus53 WoS41 Europe PMC192001 Bouillet, P., Zhang, L., Huang, D., Webb, G., Bottema, C., Shore, P., . . . Adams, J. (2001). Gene structure, alternative splicing, and chromosomal localization of pro-apoptotic Bcl-2 relative Bim. Mammalian Genome, 12(2), 163-168.
Scopus131 WoS126 Europe PMC932001 Coulthard, M., Lickliter, J., Subanesan, N., Chen, K., Webb, G., Lowry, A., . . . Boyd, A. (2001). Characterization of the EphA1 receptor tyrosine kinase: Expression in epithelial tissues. Growth Factors, 18(4), 303-317.
Scopus41 WoS40 Europe PMC242001 Travers, M., Vallance, A., Gourlay, H., Gill, C., Klein, I., Bottema, C., & Barber, M. (2001). Promoter I of the ovine acetyl-CoA carboxylase-a gene: an E-box motif at - 114 in the proximal promoter binds upstream stimulatory factor (USF)-1 and USF-2 and acts as an insulin-response sequence in differentiating adipocytes. Biochemical Journal, 359(Part 2), 273-284.
2001 Casas, E., Sonstegard, T., Barendse, W., Bennett, G., Bottema, C., Crawford, A., . . . Xu, N. (2001). Comprehensive linkage map of bovine chromosome 27. Animal Genetics, 32(2), 95-97.
Scopus4 WoS4 Europe PMC32001 Casas, E., Bennett, G., Bottema, C., Crawford, A., Kalm, E., Kappes, S., . . . Xu, N. (2001). Comprehensive linkage map of bovine chromosome 11. Animal Genetics, 32(2), 92-94.
Scopus3 WoS2 Europe PMC22000 Siebert, B., Pitchford, W., Kuchel, H., Kruk, Z., & Bottema, C. (2000). The Effect of b-carotene on desaturation of ruminant fat. Asian-Australasian Journal of Animal Sciences, 13(SUPPL. A), 185-188.
Scopus62000 Morris, C., Cullen, N., Bottema, C., Crawford, A., Hyndman, D., & Pitchford, W. (2000). Preliminary beef carcass composition data from breed crosses in a genetic marker trial. New Zealand Society of Animal Production Proceedings, 60, 113-114. 2000 Malau-Aduli, A., Edriss, M., Siebert, B., Bottema, C., Deland, M., & Pitchford, W. (2000). Estimates of genetic parameters for triacylglycerol fatty acids in beef cattle at weaning and slaughter. Journal of Animal Physiology and Animal Nutrition-Zeitschrift fur Tierphysiologie Tierernahrung und Futtermittelkunde, 83(4-5), 169-180.
Scopus13 WoS132000 Malau-Aduli, A., Siebert, B., Bottema, C., & Pitchford, W. (2000). Heterosis, sex and breed differences in the fatty acid composition of muscle phospholipids in beef cattle. Journal of Animal Physiology and Animal Nutrition-Zeitschrift fur Tierphysiologie Tierernahrung und Futtermittelkunde, 83(3), 113-120.
Scopus15 WoS142000 Malau-Aduli, A., Edriss, M., Siebert, B., Bottema, C., & Pitchford, W. (2000). Breed differences and heterosis in triacylglycerol fatty acid composition of bovine adipose tissue. Journal of Animal Physiology and Animal Nutrition-Zeitschrift fur Tierphysiologie Tierernahrung und Futtermittelkunde, 83(2), 106-112.
Scopus8 WoS92000 Malau-Aduli, A., Edriss, M., Siebert, B., Bottema, C., & Pitchford, W. (2000). Breed differences and genetic parameters for melting point, marbling score and fatty acid composition of lot-fed cattle. Journal of Animal Physiology and Animal Nutrition-Zeitschrift fur Tierphysiologie Tierernahrung und Futtermittelkunde, 83(2), 95-105.
Scopus26 WoS232000 Maddox, J. F., Andrews, A. E., Gill, C. A., & Bottema, C. D. K. (2000). Linkage and physical mapping of the sheep perforin (PRF1) gene to OAR 25. Animal Genetics, 31(3), 231-232.
Scopus1 WoS1 Europe PMC22000 Pitchford, W., & Bottema, C. (2000). Genetic effects on growth and development in Jersey and Limousin cross cattle. Asian-Australasian Journal of Animal Sciences, 13(SUPPL. A), 485-488.
Scopus12000 Duivestein, J., Pitchford, W., Bottema, C., Fassbender, R., Cusack, D., & Meldrum, R. (2000). Detection of vertical fiber hide defect (VFHD) in Hereford cattle hides by biopsy. Journal of the American Leather Chemists Association, 95(3), 92-101.
WoS11999 Koukoulas, I., Webb, G., Bottema, C., Gill, C., Johnston, C., & Aldred, G. (1999). Genomic characterization of the sheep vasopressin V1a receptor gene and promoter, with assignment to bands q23-24 of sheep chromosome 3 and cattle chromosome 5. Gene, 240(1), 183-192.
Scopus5 WoS5 Europe PMC51999 Gill, C., Davis, S., Taylor, J., Cockett, N., & Bottema, C. (1999). Construction and characterizaton of an ovine bacterial artificial chromosome library. Mammalian Genome, 10(11), 1108-1111.
Scopus19 WoS20 Europe PMC151998 Malau-Aduli, A., Siebert, B., Bottema, C., & Pitchford, W. (1998). Breed Comparison of the Fatty Acid Composition of Muscle Phospholipids in Jersey and Limousin Cattle. Journal of Animal Science, 76(3), 766-774.
Scopus113 WoS110 Europe PMC461998 Parsons, Y., Webb, G., & Bottema, C. (1998). Assignment of the growth hormone receptor gene to band q17 of the homoeologous sheep 16 and cattle 20 chromosomes. Mammalian Genome, 9(7), 599-600.
Scopus6 WoS6 Europe PMC41997 Soon, L., Bottema, C., & Breed, W. (1997). Atomic force microscopy and cytochemistry of chromatin from marsupial spermatozoa with special reference to Sminthopsis crassicaudata. Molecular Reproduction and Development, 48(3), 367-374.
Scopus8 WoS8 Europe PMC51997 Jacoby, A., Webb, G., Liu, M., Kofler, B., Hort, Y., Fathi, Z., . . . Iismaa, T. (1997). Structural organization of the mouse and human GALR1 Galanin receptor genes (Galnr and GALNR) and chromosomal localization of the mouse gene. Genomics, 45(3), 496-508.
Scopus33 WoS32 Europe PMC211997 Webb, G., Jitrapakdee, S., Bottema, C., & Wallace, J. (1997). Assignment of the pyruvate carboxylase gene to rat chromosome band 1q43 by in situ hybridization. Cytogenetics and Cell Genetics, 79(1-2), 151-152.
Scopus9 WoS8 Europe PMC21997 Malau-Aduli, A., Siebert, B., Bottema, C., & Pitchford, W. (1997). A comparison of the fatty acid composition of triacylglycerols in adipose tissue from Limousin and Jersey cattle. Australian Journal of Agricultural Research, 48(5), 715-722.
Scopus133 WoS1221997 Chapman, G., Remiszewski, J., Webb, G., Schulz, T., Bottema, C., & Rathjen, P. (1997). The Mouse Homeobox Gene, Gbx2: Genomic Organisation and Expression in Pluripotent Cells in Vitro and in Vivo. Genomics, 46(2), 223-233.
Scopus38 WoS38 Europe PMC261997 Pal, S., Bottema, C. D. K., & Roach, P. D. (1997). The effects of polyunsaturated fatty acids and vitamins on the expression of the low density lipoprotein receptor in human HepG2 hepatoma cells. ATHEROSCLEROSIS, 134(1-2), 361.
1996 Andersson, L., Bottema, C., Broad, T., Gill, T., & Graves, J. (1996). Comparative Genome Organisation of Vertebrates. Mammalian Genome, 7(10), 717-734.
Scopus137 WoS117 Europe PMC761996 Bernard, O., Burkitt, V., Webb, G., Bottema, C., Nicholl, J., Sutherland, G., & Matthew, P. (1996). Structure and chromosomal localization of the genomic locus encoding the Kiz1 LIM-Kinase gene. Genomics, 35(3), 593-596.
Scopus13 WoS12 Europe PMC101995 Bottema, C. (1995). Genetic testing comes of age. Outlook on Agriculture, 24(4), 203-205. 1993 Dutton, C. M., Bottema, C. D. K., & Sommer, S. S. (1993). Alu repeats in the human factor IX gene: The rate of polymorphism is not substantially elevated. Human Mutation, 2(6), 468-472.
Scopus2 WoS3 Europe PMC31993 Bottema, C., & Sommer, S. (1993). PCR amplification of specific alleles: Rapid detection of known mutations and polymorphisms. Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 288(1), 93-102.
Scopus106 WoS105 Europe PMC681993 Gostout, B., Vielhaber, E., Ketterling, R. P., Yoon, H. S., Bottema, C. D. K., Kasper, C. K., . . . Sommer, S. S. (1993). Germline mutations in the factor IX gene: A comparison of the pattern in caucasians and non-caucasians. Human Molecular Genetics, 2(3), 293-298.
Scopus23 WoS26 Europe PMC201993 Bottema, C. D. K., Ketterling, R. P., Vielhaber, E., Yoon, H. S., Gostout, B., Jacobson, D. P., . . . Sommer, S. S. (1993). The pattern of spontaneous germ-line mutation: relative rates of mutation at or near CpG dinucleotides in the factor IX gene. Human Genetics, 91(5), 496-503.
Scopus46 WoS47 Europe PMC291993 Bottema, C., Sarkar, G., Cassady, J., Ii, S., Dutton, C., & Sommer, S. (1993). Polymerase Chain Reaction Amplification of Specific Alleles: A General Method of Detection of Mutations, Polymorphisms, and Haplotypes. Methods in Enzymology, 218(C), 388-402.
Scopus65 WoS64 Europe PMC431993 Ketterling, R. P., Vielhaber, E., Bottema, C. D. K., Schaid, D. J., Cohen, M. P., Sexauer, C. L., & Sommer, S. S. (1993). Germ-line origins of mutation in families with hemophilia B: The sex ratio varies with the type of mutation. American Journal of Human Genetics, 52(1), 152-166.
Scopus73 WoS78 Europe PMC491992 Sommer, S. S., Bowie, E. J. W., Ketterling, R. P., & Bottema, C. D. K. (1992). Missense mutations and the magnitude of functional deficit: the example of factor IX. Human Genetics, 89(3), 295-297.
Scopus16 WoS18 Europe PMC141992 Sommer, S. S., Groszbach, A. R., & Bottema, C. D. K. (1992). PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes. BioTechniques, 12(1), 82-87.
Scopus196 WoS202 Europe PMC1141992 KETTERLING, R., BOTTEMA, C., VIELHABER, E., & SOMMER, S. (1992). DIRECT CARRIER DETECTION FOR HEMOPHILIA-B - EXPERIENCE WITH 140 FAMILIES. TRANSFUSION, 32(5), 486. 1991 Bottema, C. D. K., Ketterling, R. P., Ii, S., Yoon, H. S., Phillips, J. A., & Sommer, S. S. (1991). Missense mutations and evolutionary conservation of amino acids: Evidence that many of the amino acids in factor IX function as 'spacer' elements. American Journal of Human Genetics, 49(4), 820-838.
Scopus89 WoS101 Europe PMC631991 Bottema, C. D. K., Bottema, M. J., Ketterling, R. P., Yoon, H. S., Janco, R. L., Phillips, J. A., & Sommer, S. S. (1991). Why does the human factor IX gene have a G + C content of 40%?. American Journal of Human Genetics, 49(4), 839-850.
Scopus43 WoS50 Europe PMC321991 Ketterling, R. P., Bottema, C. D. K., Koeberl, D. D., Ii, S., & Sommer, S. S. (1991). T<sup>296</sup>→M, a common mutation causing mild hemophilia B in the Amish and others: founder effect, variability in factor IX activity assays, and rapid carrier detection. Human Genetics, 87(3), 333-337.
Scopus25 WoS30 Europe PMC201991 BOTTEMA, C., KETTERLING, R., II, S., YOON, H., AXELSEN, P., PHILLIPS, J., & SOMMER, S. (1991). MISSENSE MUTATIONS AND EVOLUTIONARY CONSERVATION OF AMINO-ACIDS - EVIDENCE THAT MANY OF THE AMINO-ACIDS IN FACTOR-IX FUNCTION AS SPACER ELEMENTS. AMERICAN JOURNAL OF HUMAN GENETICS, 49(4), 462.
WoS11991 Ketterling, R. P., Bottema, C. D. K., Phillips, J. A., & Sommer, S. S. (1991). Evidence that descendants of three founders constitute about 25% of hemophilia B in the united states. Genomics, 10(4), 1093-1096.
Scopus46 WoS51 Europe PMC391991 BOTTEMA, C., PHILLIPS, J., & SOMMER, S. (1991). MISSENSE MUTATIONS AND MILD HEMOPHILIA B. AMERICAN JOURNAL OF HUMAN GENETICS, 49(4), 15. 1991 BOTTEMA, C., BOTTEMA, M., KETTERLING, R., YOON, H., JANCO, R., PHILLIPS, J., & SOMMER, S. (1991). WHY DOES THE HUMAN FACTOR-IX GENE HAVE A G+C CONTENT OF 39 PERCENT. AMERICAN JOURNAL OF HUMAN GENETICS, 49(4), 494. 1990 Bottema, C. D. K., Koeberl, D. D., Ketterling, R. P., Bowie, E. J. W., Taylor, S. A. M., Lillicrap, D., . . . Sommer, S. S. (1990). A past mutation at Isoleucine<sup>397</sup> is now a common cause of moderate/mild haemophilia B. British Journal of Haematology, 75(2), 212-216.
Scopus25 WoS35 Europe PMC281990 Bottema, C. D. K., Ketterling, R. P., Koeberl, D. D., Taylor, S. A., & Sommer, S. S. (1990). Mutations at arginine residues in two Asian hemophilia B patients. Nucleic Acids Research, 18(7), 1924.
Scopus5 WoS8 Europe PMC81990 Bottema, C. D. K., Ketterling, R. P., Yoon, H. S., & Sommer, S. S. (1990). The pattern of factor IX germ-line mutation in Asians is similar to that of Caucasians. American Journal of Human Genetics, 47(5), 835-841.
Scopus36 WoS48 Europe PMC381990 Koeberl, D. D., Bottema, C. D. K., & Sommer, S. S. (1990). Comparison of direct and indirect methods of carrier detection in an X-linked disease. American Journal of Medical Genetics, 35(4), 600-608.
Scopus4 WoS6 Europe PMC41990 Koeberl, D. D., Bottema, C. D. K., Ketterling, R. P., Bridge, P. J., Lillicrap, D. P., & Sommer, S. S. (1990). Mutations causing hemophilia B: Direct estimate of the underlying rates of spontaneous germ-line transitions, transversions, and deletions in a human gene. American Journal of Human Genetics, 47(2), 202-217.
Scopus109 WoS142 Europe PMC951990 Sarkar, G., Cassady, J., Bottema, C., & Sommer, S. (1990). Characterization of polymerase chain reaction amplification of specific alleles. Analytical Biochemistry, 186(1), 64-68.
Scopus140 WoS153 Europe PMC791990 Koeberl, D. D., Bottema, C. D. K., Sarkar, G., Ketterling, R. P., Chen, S. H., & Sommer, S. S. (1990). Recurrent nonsense mutations at arginine residues cause severe hemophilia B in unrelated hemophiliacs. Human Genetics, 84(5), 387-390.
Scopus23 WoS34 Europe PMC251990 SOMMER, S., BOTTEMA, C., KOEBERL, D., & KETTERLING, R. (1990). DELINEATION BY DIRECT SEQUENCING OF THE MUTATION IN 62 PERSONS WITH HEMOPHILIA-B AND DIRECT CARRIER TESTING IN 58 AT-RISK WOMEN. TRANSFUSION, 30(3), 281. 1989 BOTTEMA, C. D. K., KOEBERL, D. D., & SOMMER, S. S. (1989). DIRECT CARRIER TESTING IN 14 FAMILIES WITH HAEMOPHILIA-B. LANCET, 2(8662), 526-529.
WoS34 Europe PMC201989 SOMMER, S. S., CASSADY, J. D., SOBELL, J. L., & BOTTEMA, C. D. K. (1989). A Novel Method for Detecting Point Mutations or Polymorphisms and Its Application to Population Screening for Carriers of Phenylketonuria. Mayo Clinic Proceedings, 64(11), 1361-1372.
Scopus126 WoS132 Europe PMC731989 Koeberl, D. D., Bottema, C. D. K., Buerstedde, J. M., & Sommer, S. S. (1989). Functionally important regions of the factor IX gene have a low rate of polymorphism and a high rate of mutation in the dinucleotide CpG. American Journal of Human Genetics, 45(3), 448-457.
Scopus79 WoS106 Europe PMC781989 Bottema, C. D. K., Ketterling, R. P., Cho, H. I., & Sommer, S. S. (1989). Hemophilia B in a male with a four-base insertion that arose in the germline of his mother. Nucleic Acids Research, 17(23), 10139.
Scopus9 WoS13 Europe PMC121989 SOMMER, S., KOEBERL, D., BOTTEMA, C., & BUERSTEDDE, J. (1989). FUNCTIONALLY IMPORTANT REGIONS OF THE FACTOR-IX GENE HAVE A LOW RATE OF POLYMORPHISM AND A HIGH-RATE OF MUTATION IN THE DINUCLEOTIDE CPG. THROMBOSIS AND HAEMOSTASIS, 62(1), 203. 1986 Miller, S. C., Bottema, C. D. K., Stathis, P. A., Tokés, L. G., & Feldman, D. (1986). Unexpected presence of estrogens in culture medium supplements: Subsequent metabolism by the yeast sacchromyces cerevisiae. Endocrinology, 119(3), 1362-1369.
Scopus13 WoS16 Europe PMC101985 Parks, L., Bottema, C., Rodriguez, R., & Lewis, T. (1985). [18] Yeast Sterols: Yeast Mutants as Tools for the Study of Sterol Metabolism. Methods in Enzymology, 111(C), 333-346.
Scopus71 WoS80 Europe PMC571985 Bottema, C., Rodriguez, R., & Parks, L. (1985). Influence of sterol structure on yeast plasma membrane properties. BBA - Biomembranes, 813(2), 313-320.
Scopus26 WoS26 Europe PMC131985 Rodriguez, R., Low, C., Bottema, C., & Parks, L. (1985). Multiple functions for sterols in Saccharomyces cerevisiae. Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, 837(3), 336-343.
Scopus185 WoS205 Europe PMC1211984 MCCAMMON, M. T., HARTMANN, M. A., BOTTEMA, C. D. K., & PARKS, L. W. (1984). STEROL METHYLATION IN SACCHAROMYCES-CEREVISIAE. JOURNAL OF BACTERIOLOGY, 157(2), 475-483.
WoS45 Europe PMC331983 Bottema, C. D. K., McLean-Bowen, C. A., & Parks, L. W. (1983). Role of sterol structure in the thermotropic behavior of plasma membranes of Saccharomyces cerevisiae. BBA - Biomembranes, 734(2), 235-248.
Scopus28 WoS361983 BOTTEMA, C., & PARKS, L. (1983). Effect of sterol structure on the physical-properties and function of plasma-membranes from yeast. Federation proceedings, 42(7), 1772. 1982 Parks, L., McLean-Bowen, C., Bottema, C., Taylor, F., Gonzales, R., Jensen, B., & Ramp, J. (1982). Aspects of sterol metabolism in the yeast Saccharomyces cerevisiae and in Phytophthora. Lipids, 17(3), 187-196.
Scopus12 WoS18 Europe PMC61981 PARKS, L., MCLEANBOWEN, C., & BOTTEMA, C. (1981). EFFECTS OF LIPID ALTERATIONS ON MEMBRANE-PROPERTIES OF S-CEREVISIAE. FEDERATION PROCEEDINGS, 40(6), 1561. 1980 Bottema, C. K., & Parks, L. W. (1980). Sterol analysis of the inner and outer mitochondrial membranes in yeast. Lipids, 15(12), 987-992.
Scopus15 WoS151978 Bottema, C., & Parks, L. (1978). Δ¹⁴-sterol reductase in Saccharomyces cerevisiae. Biochimica et Biophysica Acta - Lipids and Lipid Metabolism, 531(3), 301-307.
Scopus29 WoS37 Europe PMC12Bottema, C., Do, H., Chen, T., Skirving, R., Petrovski, K., & Williams, J. L. (n.d.). Hue, et al. (2021) Colostrum source and passive immunity transfer in dairy bull calves. Journal of Dairy Science paper Supplementary Files.
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Current Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2022 Co-Supervisor Mapping and identification of candidate genes controlling fat related production traits in cattle Doctor of Philosophy Doctorate Full Time Mr Junpeng Yao 2020 Co-Supervisor Effects of colostrum intake on fertility and milk production in dairy cattle Doctor of Philosophy Doctorate Part Time Dr Rebel Skirving 2017 Principal Supervisor Gene editing in Brahman Cattle to produce polled animals Doctor of Philosophy Doctorate Full Time Miss Johanna Ellen Aldersey -
Past Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2017 - 2021 Principal Supervisor Transfer of Colostrum Components to Newborn Calves Doctor of Philosophy Doctorate Full Time Mrs Hue Thi Do 2016 - 2020 Principal Supervisor KIT Mutations in Australian Canine Mast Cell Tumours and Correlations with Patient Prognostic Factors Doctor of Philosophy Doctorate Full Time Miss Vanessa Tamlin 2015 - 2021 Co-Supervisor Application of whole genome sequencing to Australia-wide collections of extraintestinal pathogenic Escherichia coli from companion animals and commensal E. coli from pigs Doctor of Philosophy Doctorate Part Time Ms Amanda Kidsley 2013 - 2014 Co-Supervisor Genetic diversity and measuring genetic parameters for economically import traits in Zambian cattle Master of Philosophy Master Full Time Mr Ellison Musimuko 2012 - 2012 Co-Supervisor Isolation and Characterisation of Tannin-Resistant Bacteria from the Rumen of Feral Goats and Camels Master of Science Master Full Time Ms Anita Sardiana Tjakradidjaja 2011 - 2017 Principal Supervisor Progressing a Scoring Scheme for Intervertebral Disc Clacification Screening in Dachshund Dogs Master of Philosophy Master Part Time Dr Alana Rosenblatt 2009 - 2016 Principal Supervisor Molecular Genetics of Residual Feed Intake and Mitochondrial Function in Cattle Doctor of Philosophy Doctorate Full Time Mrs Nadiatur Akmar Zulkifli 2007 - 2012 Principal Supervisor Quantitative Trait Loci and Genes Affecting Beef Tenderness Doctor of Philosophy Doctorate Full Time Mr Lei Yao Chang 2007 - 2012 Principal Supervisor Genomic Approach to Understanding Variation in Bovine Fat Colour Doctor of Philosophy Doctorate Full Time Mr Rugang Tian 2007 - 2016 Principal Supervisor Consequences of Selection for Residual Feed Intake Doctor of Philosophy Doctorate Full Time Mr David Lines 2007 - 2011 Principal Supervisor Molecular Genetics of Cattle Muscularity Master of Agricultural Science Master Full Time Ms Irida Novianti 2006 - 2012 Principal Supervisor Genetic Independence of Fat Depots in Cattle Doctor of Philosophy Doctorate Full Time Mr Andrew Egarr 2004 - 2010 Principal Supervisor Molecular Characterisation of Primary Wool Follicle Initiation in Merino Sheep Doctor of Philosophy Doctorate Part Time Dr Hayley McGrice 2003 - 2008 Principal Supervisor Identification and Characterization of Genetic Markers and Metabolic Pathways Controlling Net Feed Efficiency in Beef Cattle Doctor of Philosophy Doctorate Full Time Mr Madan Naik 2003 - 2007 Co-Supervisor Multiple Trait Analysis for Genetic Mapping of Quantitative Trait Loci for Carcass and Beef Quality Doctor of Philosophy Doctorate Full Time Mr Ali Esmailizadeh Koshkoih 1999 - 2003 Co-Supervisor Genetics of Growth and Development in Cattle Doctor of Philosophy Doctorate Full Time Mr Raphael Afolayan 1998 - 2004 Co-Supervisor Genomics of Feed Efficiency for Livestock Doctor of Philosophy Doctorate Full Time Dr Michelle Hebart 1998 - 2002 Principal Supervisor DISCOVERY, ANALYSIS, AND UTILITY OF BOVINE SINGLE NUCLEOTIDE POLYMORPHISMS Doctor of Philosophy Doctorate Part Time Miss Gabrielle Sellick 1995 - 2010 Co-Supervisor Comparative Analysis of Two Attachment Variants of Butyrivibrio Fibrisolvens Doctor of Philosophy Doctorate Full Time Mr Djarot Sasongko Hami Seno
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