Dr Xiaohui Xing

Xiaohui Xing
Postdoctoral Research Fellow
School of Agriculture, Food and Wine
Faculty of Sciences

Dr. Xiaohui Xing received his Ph.D. degree from the Department of Food Science at the University of Guelph in 2014 in Ontario, Canada. Xiaohui conducted all his Ph.D. experiments at a federal carbohydrate chemistry research lab of Agriculture Agri-Food Canada (AAFC), advised collaboratively by carbohydrate chemists Prof. Steve W. Cui and Prof. H. Douglas Goff. After his postdoc work with carbohydrate biochemist Prof. Vincent Bulone at the Royal Institute of Technology (KTH) in Stockholm, Xiaohui followed Prof. Vincent Bulone to the University of Adelaide in August 2016 and since then has worked as a postdoc research fellow at the ARC Center of Excellence in Plant Cell Wall. He also works as a postdoctoral research scientist at Adelaide Glycomics, a complex carbohydrate research center founded by Prof. Vincent Bulone in August 2016. As the first researcher working in Adelaide Glycomics, Xiaohui has set up and been responsible for the detailed structural characterisation of complex carbohydrates in the new research center.

Xiaohui’s research interests lie in discovering novel complex carbohydrates from natural products (e.g., higher plants, fungi, yeast, bacteria, microalgae), elucidating their detailed structural features, investigating their application as bioactive agents, dietary fiber, and biomaterials (e.g., encapsulation material), and understanding their structure-function relationships.

Xiaohui’s expertise includes but not limited to:
(1) Structural elucidation of polysaccharides using 2D NMR spectroscopy (COSY, TOCSY, HMBC, HMQC, NOESY, & ROESY).
(2) Glycosidic linkage analysis (methylation-GC-MS analysis in the form of partially methylated alditol acetates) of purified carbohydrates or mixture of cell wall polysaccharides from higher plants, fungi, yeast, bacteria, microalgae, and other natural sources. Have ability to conduct linkage analysis of carbohydrate samples containing not only neutral sugars but also uronic acids, amino sugars, sulfated sugars, and others.
(3) Monosaccharide composition analysis of carbohydrates using GC-MS-FID (in the form of alditol acetates) or using high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
(4) Structural characterization of carbohydrates using FTIR-ATR spectroscopy.
(5) Determination of molecular weight and conformational parameters of polysaccharides using high performance size exclusion chromatography (HPSEC) coupled with multiple detectors (e.g., RI, UV, viscometer, laser light scattering detectors).
(6) Interpretation of high-resolution tandem mass spectra (e.g., high resolution LC-MS) of oligosaccharides for accurate molecular mass and detailed structural information.
(7) Structural characterisation of polysaccharide using enzymatic hydrolysis followed by MALDI-tof-MS or HPACE-PAD analysis.
(8) Conformational study of polysaccharide in dilute solution using multiple-angle static and dynamic light scattering, viscometry, and rheology.
(9) Quantitative analysis of starch, protein, neutral sugar, uronic acids, and amino sugars using UV-Vis spectroscopy.

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Dr Xiaohui Xing

Dr. Xiaohui Xing received his Ph.D. degree from the Department of Food Science at the University of Guelph in 2014 in Ontario, Canada. Xiaohui conducted all his Ph.D. experiments at a federal carbohydrate chemistry research lab of Agriculture Agri-Food Canada (AAFC), advised collaboratively by carbohydrate chemists Prof. Steve W. Cui and Prof. H. Douglas Goff. After his postdoc work with carbohydrate biochemist Prof. Vincent Bulone at the Royal Institute of Technology (KTH) in Stockholm, Xiaohui followed Prof. Vincent Bulone to the University of Adelaide in August 2016 and since then has worked as a postdoc research fellow at the ARC Center of Excellence in Plant Cell Wall. He also works as a postdoctoral research scientist at Adelaide Glycomics, a complex carbohydrate research center founded by Prof. Vincent Bulone in August 2016. As the first researcher working in Adelaide Glycomics, Xiaohui has set up and been responsible for the detailed structural characterisation of complex carbohydrates in the new research center.

Xiaohui’s research interests lie in discovering novel complex carbohydrates from natural products (e.g., higher plants, fungi, yeast, bacteria, microalgae), elucidating their detailed structural features, investigating their application as bioactive agents, dietary fiber, and biomaterials (e.g., encapsulation material), and understanding their structure-function relationships.

Xiaohui’s expertise includes but not limited to:
(1) Structural elucidation of polysaccharides using 2D NMR spectroscopy (COSY, TOCSY, HMBC, HMQC, NOESY, & ROESY).
(2) Glycosidic linkage analysis (methylation-GC-MS analysis in the form of partially methylated alditol acetates) of purified carbohydrates or mixture of cell wall polysaccharides from higher plants, fungi, yeast, bacteria, microalgae, and other natural sources. Have ability to conduct linkage analysis of carbohydrate samples containing not only neutral sugars but also uronic acids, amino sugars, sulfated sugars, and others.
(3) Monosaccharide composition analysis of carbohydrates using GC-MS-FID (in the form of alditol acetates) or using high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).
(4) Structural characterization of carbohydrates using FTIR-ATR spectroscopy.
(5) Determination of molecular weight and conformational parameters of polysaccharides using high performance size exclusion chromatography (HPSEC) coupled with multiple detectors (e.g., RI, UV, viscometer, laser light scattering detectors).
(6) Interpretation of high-resolution tandem mass spectra (e.g., high resolution LC-MS) of oligosaccharides for accurate molecular mass and detailed structural information.
(7) Structural characterisation of polysaccharide using enzymatic hydrolysis followed by MALDI-tof-MS or HPACE-PAD analysis.
(8) Conformational study of polysaccharide in dilute solution using multiple-angle static and dynamic light scattering, viscometry, and rheology.
(9) Quantitative analysis of starch, protein, neutral sugar, uronic acids, and amino sugars using UV-Vis spectroscopy.

 

 

Xiaohui’s research interests lie in discovering novel complex carbohydrates from natural products (e.g., higher plants, fungi, yeast, bacteria, microalgae), elucidating their detailed structural features, investigating their application as bioactive agents, dietary fiber, and biomaterials (e.g., encapsulation material), and understanding their structure-function relationships.

 

Xiaohui’s expertise includes but not limited to:

(1) Structural elucidation of polysaccharides using 2D NMR spectroscopy (COSY, TOCSY, HMBC, HMQC, NOESY, & ROESY).

(2) Glycosidic linkage analysis (methylation-GC-MS analysis in the form of partially methylated alditol acetates) of purified carbohydrates or mixture of cell wall polysaccharides from higher plants, fungi, yeast, bacteria, microalgae, and other natural sources. Have ability to conduct linkage analysis of carbohydrate samples containing not only neutral sugars but also uronic acids, amino sugars, sulfated sugars, and others.

(3) Monosaccharide composition analysis of carbohydrates using GC-MS-FID (in the form of alditol acetates) or using high performance anion exchange chromatography coupled with pulsed amperometric detection (HPAEC-PAD).

(4) Structural characterization of carbohydrates using FTIR-ATR spectroscopy.

(5) Determination of molecular weight and conformational parameters of polysaccharides using high performance size exclusion chromatography (HPSEC) coupled with multiple detectors (e.g., RI, UV, viscometer, laser light scattering detectors).

(6) Interpretation of high-resolution tandem mass spectra (e.g., high resolution LC-MS) of oligosaccharides for accurate molecular mass and detailed structural information.

(7) Structural characterisation of polysaccharide using enzymatic hydrolysis followed by MALDI-tof-MS or HPACE-PAD analysis.

(8) Conformational study of polysaccharides in dilute solution using multiple-angle static and dynamic light scattering, viscometry, and rheology.

(9) Quantitative analysis of starch, protein, neutral sugar, uronic acids, and amino sugars using UV-Vis spectroscopy.

Language Competencies

Language Competency
Chinese (Cantonese) Can read, write and peer review
Chinese (Mandarin) Can read, write, speak, understand spoken and peer review
English Can read, write, speak, understand spoken and peer review

Education

Date Institution name Country Title
2009 - 2014 University of Guelph Canada Ph.D. degree in Food Science (focused on Food Carbohydrate Chemistry)
2008 - 2009 Northwest A&F University China Ph.D. course study (Food Science)
2005 - 2008 Northwest A&F University China Master's degree in Cereal, Oil, and Plant Protein Engineering
2001 - 2005 Northwest A&F University China Batchelor's degree in Food Science and Engineering

Research Interests

Analytical Biochemistry, Analytical Chemistry, Biochemistry & Molecular Biology, Biotechnology, Food Chemistry and Molecular Gastronomy, Food Engineering, Food Science, Macromolecular and Materials Chemistry, Medical Biochemistry: Carbohydrates, Medicinal and Biomolecular Chemistry, Natural Products Chemistry, Organic Chemistry, Physical Chemistry, Physical Chemistry of Materials, Solution Chemistry

Journals

Year Citation
2017 XING, Q., XING, X., ZHANG, Z., HU, X. & LIU, F. (2017). A comparative study of the nutritional values, volatile compounds, and sensory qualities of pea pastes cooked in iron pot and clay pot. Journal of Food Processing and Preservation, Online, 1-6. 10.1111/jfpp.13328
2017 Yu, L., Yakubov, G., Zeng, W., Xing, X., Stensone, J., Bulone, V. & Stokes, J. (2017). Multi-layer mucilage of Plantago ovata seeds: Rheological differences arise from variations in arabinoxylan side chains. Carbohydrate Polymers, 165, 132-141. 10.1016/j.carbpol.2017.02.038
2015 Xing, X., CUI, Steve W., , NIE, Shaoping, , PHIllIPS, Glyn O., , GOFF, H. Douglas, & WANG, Qi, (2015). Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan (R)) Part II. Fine structures of O-acetylated residues. Carbohydrate Polymers, 117, 422-433. 10.1016/j.carbpol.2014.08.121
2015 Xing, X., Cui, S., Nie, S., Phillips, G., Goff, H. & Wang, Q. (2015). Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan®): Part V. Fractionation and structural heterogeneity of different fractions. Bioactive Carbohydrates and Dietary Fibre, 5, 2, 106-115. 10.1016/j.bcdf.2014.12.005
2014 Xing, X., Cui, S., Nie, S., Phillips, G., Goff, H. & Wang, Q. (2014). Study on Dendrobium officinale O-acetyl-glucomannan (Dendronan®): Part I. Extraction, purification, and partial structural characterization. Bioactive Carbohydrates and Dietary Fibre, 4, 1, 74-83. 10.1016/j.bcdf.2014.06.004
2013 Guo, Q., Wang, Q., Cui, S., Kang, J., Hu, X., Xing, X. & Yada, R. (2013). Conformational properties of high molecular weight heteropolysaccharide isolated from seeds of Artemisia sphaerocephala Krasch. Food Hydrocolloids, 32, 1, 155-161. 10.1016/j.foodhyd.2012.12.014
2013 Xing, X., Cui, S., Nie, S., Phillips, G., Douglas Goff, H. & Wang, Q. (2013). A review of isolation process, structural characteristics, and bioactivities of water-soluble polysaccharides from Dendrobium plants. Bioactive Carbohydrates and Dietary Fibre, 1, 2, 131-147. 10.1016/j.bcdf.2013.04.001
2013 Hu, X., Xing, X. & Zhen, H. (2013). Enzyme deactivation treatments did not decrease the beneficial role of oat food in intestinal microbiota and short-chain fatty acids: an in vivo study. Journal of the Science of Food and Agriculture, 93, 3, 504-508. 10.1002/jsfa.5808
2011 Hu, X., Yan, J. & Xing, X. (2011). The effects of steaming and roasting treatments on lipase activity and nutritional components of “oat rice” (OR): the peeled naked oat (Avena nuda) kernels. Agricultural Sciences, 02, 02, 56-60. 10.4236/as.2011.22009
2011 Hu, X., Xing, X., Zhang, Z., Wu, R., Guo, Q., Cui, S. W. & Wang, Q. (2011). Antioxidant effects of Artemis sphaerocephala Krasch. gum, on streptozotocin-induced type 2 diabetic rats. Food Hydrocolloids, 25, 2, 207-213. 10.1016/j.foodhyd.2009.12.006
2010 Wu, R., Li, Z., Yang, J., Xing, X., Shao, D. & Xing, K. (2010). Mutagenesis induced by high hydrostatic pressure treatment: a useful method to improve the bacterial cellulose yield of a Gluconoacetobacter xylinus strain. Cellulose, 17, 2, 399-405. 10.1007/s10570-009-9388-8
2009 Xing, X., Zhang, Z., Hu, X., Wu, R. & Xu, C. (2009). Antidiabetic effects of Artemisia sphaerocephala Krasch. gum, a novel food additive in China, on streptozotocin-induced type 2 diabetic rats. Journal of Ethnopharmacology, 125, 3, 410-416. 10.1016/j.jep.2009.07.021
2009 Xing, X., YAN, J., ZHANG, Z. & HU, X. (2009). Characterization of Artemis sphaerocephala Krasch. seed powder. Journal of the Chinese Cereals and Oils Association, 24, 2, 56-59.
2008 WU, R., DU, S., LI, Z., XING, X., SHAO, D., FAN, Y. ... BU, L. (2008). Optimization of bacterial cellulose fermentation medium and observation of bacterial cellulose ultra-micro-structure. Chinese Journal of Biotechnology, 24, 6, 1068-1074.

Book Chapters

Year Citation
2010 HU, X. & XING, X. (2010). Medical properties of Shahao in China. In Awaad, Amani S, Govil, J. N. & Singh, V. K. (Eds.), Recent Progress in Medicinal Plants (pp. 215-233). Studium Press.
Position
Postdoctoral Research Fellow
Campus
Waite
Building
Building 20
Org Unit
School of Agriculture, Food and Wine

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