Victoria Pederick

Dr Victoria Pederick

Research Associate

School of Biological Sciences

Faculty of Sciences


Dr. Pederick is a postdoctoral research within the Research Centre for Infectious Diseases, where she is working to characterise the molecular mechanisms of zinc acquisition in bacterial pathogens.

Metals ions are essential for bacterial growth, serving structural and catalytic roles in the cell. However, in excess, they also exert significant toxicity, necessitating the strict control of their acquisition. Metalloproteomic analyses indicate that at least 6% of all bacterial proteins require the transition metal ion, zinc [Zn(II)]. Due to the importance of Zn(II) for bacteria, the human immune system manipulates the bioavailability of the metal ion to limit or prevent bacterial infections. To overcome the reduced Zn(II) bioavailability, bacteria utilise high affinity membrane transporters to satisfy their metal ion requirement. My research focuses on the Zn(II) acquisition pathways employed by the human pathogens Pseudomonas aeruginosa and Streptococcus pneumoniae.

 

1. Zinc acquisition in Streptococcus pneumoniae
Streptococcus pneumoniae (the pneumococcus) is a globally significant human pathogen, responsible for a broad range of diseases including pneumonia, meningitis, bacteremia and otitis media. Within the pneumococcus, Zn(II) is the second most abundant transition metal ion. To acquire Zn(II), the bacterium utilises an ATP-binding cassette importer, AdcBC, and two high affinity Zn(II)-specific solute-binding proteins, AdcA and AdcAII, for transport of the metal ion across the membrane. In response to Zn(II) limitation, adcAII is significantly up-regulated, together with its co-transcribed gene, phtD. PhtD is one of four highly conserved polyhistidine triad (Pht) proteins (PhtA, PhtB, PhtD and PhtE), which are present in the cell wall of the pneumococcus. Each protein features 5 or 6 histidine triad motifs (HXXHXH) that are predicted to bind Zn(II) ions. Zinc acquisition by AdcAII under Zn(II)-limiting conditions has been shown to be dependent on the Pht proteins, with the four Pht proteins sharing overlapping functionality. Further research is required to better understand the role of each of these proteins in pneumococcal Zn(II) acquisition.

 

2. Characterisation of novel zinc acquisition mechanisms in Pseudomonas aeruginosa
Pseudomonas aeruginosa infections are the leading cause of death and disease in children with cystic fibrosis (CF). Within the CF lung, much of the Zn(II) is bound by the human protein calprotectin to prevent bacterial colonisation. To overcome this, P. aeruginosa is predicted to utilise multiple import mechanisms to scavenge Zn(II). My recent study characterising the ZnuABC importer from P. aeruginosa revealed it to hold a major role in Zn(II) acquisition, with a mutant strain lacking the znuA gene exhibiting an ~60% reduction in cellular Zn(II) content. RNA sequencing analysis of this strain identified a number of additional, previously uncharacterised acquisition systems which likely contribute to Zn(II) acquisition by the bacterium. Together my research is seeking to better understand the role and relative contribution of these putative transport systems to P. aeruginosa Zn(II) acquisition.

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  • Appointments

    Date Position Institution name
    2015 Research Associate The University of Adelaide
  • Awards and Achievements

    Date Type Title Institution Name Country Amount
    2016 Award Travel Grant UK Biochemical Society £650
    2013 Award Student Poster Prize Australian Society for Microbiology
    2012 Award Oral Presentation Prize School of Molecular and Biomedical Sciences Postgraduate Symposium
    2012 Award Student Poster Prize Adelaide Protein Group
    2012 Award PXE Student Conference Fellowship FEBS-ABC 2012
    2011 Scholarship Australian Postgraduate Award The University of Adelaide
    2011 Scholarship Postgraduate Studentship Award Australian Cystic Fibrosis Research Trust
    2009 Award Summer Research Scholarship Cancer Council of South Australia
  • Education

    Date Institution name Country Title
    2011 - 2013 The University of Adelaide Australia PhD
    2010 The University of Adelaide Australia B. Sci. (Honours in Microbiology and Immunology)
    2007 - 2009 The University of Adelaide Australia B. Sci. (Molecular Biology)
  • Research Interests

2013 - Channel 7 Children's Research Foundation Grant ($31,000)
           "The role of a Type 1 secretion system in Pseudomonas aeruginosa biofilm formation" [CIA]

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  • Memberships

    Date Role Membership Country
    2011 - ongoing Member Australian Society for Biochemistry and Molecular Biology
    2011 - ongoing Member UK Biochemical Society
    2011 - 2015 Member Australian Society for Microbiology
    2011 - 2012 Member Australian Society for Medical Research
    2010 - ongoing Member Adelaide Protein Group
  • Position: Research Associate
  • Phone: 83134671
  • Email: victoria.pederick@adelaide.edu.au
  • Fax: 8313 4362
  • Campus: North Terrace
  • Building: Molecular Life Sciences, floor 4
  • Org Unit: Molecular and Biomedical Science

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