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Vicky Staikopoulos

Vicky Staikopoulos
Laboratory Manager - RA
Adelaide Medical School
Faculty of Health and Medical Sciences

Vicky Staikopoulos.
Vicky is currently enrolled as a PhD student within the Neuroimmunopharmacology Laboratory (School Medicine/ Faculty Health Sciences), headed by supervisor Prof. Mark Hutchinson. Vicky is also a member of the ARC Centre of Excellence for Nanoscale Biophotonics (Origins of Sensations biological theme).

Title of her PhD research project is: The role of nitroxyl (HNO) in neuropathic pain signalling and development of morphine tolerance.

Vicky also currently holds a part-time position as Laboratory Manager/Research Assistant within the same lab, and prior to starting her PhD,worked in a similar capacity within various labs from the Universities of Adelaide and Melbourne for the last 16 years.

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Vicky Staikopoulos

Vicky Staikopoulos.
Vicky is currently enrolled as a PhD student within the Neuroimmunopharmacology Laboratory (School Medicine/ Faculty Health Sciences), headed by supervisor Prof. Mark Hutchinson. Vicky is also a member of the ARC Centre of Excellence for Nanoscale Biophotonics (Origins of Sensations biological theme).

Title of her PhD research project is: The role of nitroxyl (HNO) in neuropathic pain signalling and development of morphine tolerance.

Vicky also currently holds a part-time position as Laboratory Manager/Research Assistant within the same lab, and prior to starting her PhD,worked in a similar capacity within various labs from the Universities of Adelaide and Melbourne for the last 16 years.

Research Question

 

 

Is the development of neuropathic pain and/or the loss of analgesic effects of morphine dependent upon the concentrations of central nitric oxide and nitroxyl, and are these effects mediated by the TLR4 receptor pathway?

 

BACKGROUND

  • painNeuropathic pain, the most diagnosed type of chronic pain in Australia affects approximately 7% of the population with highest                                                                                                                                                                                                             prevalence in patients aged 45-64 and sex difference varying depending on the underlying disease state (Pollack, A, et al., 2013). The total cost of chronic pain to the Australian economy was reported to be $34 billion, including $7 billion in health system costs (Access Economics, 2007).
  • Neuropathic pain is defined as pain caused by a lesion of the peripheral or central nervous system (CNS) and can develop either post-surgically or from a variety of disease states ranging from diabetic neuropathy through to post-traumatic spinal cord injury. It is characterized by the presence of allodynia (pain generated from non-noxious stimuli) and hyperalgesia (heightened sense of pain to noxious stimuli) with patients frequently presenting with symptoms such as burning pain, stabbing pain or pins and needles. 
  • Pain normally serves to guard against tissue injury, as it is imperative that the body is aware of potentially damaging stimuli. It also allows us to protect the damaged area as to facilitate tissue repair after injury.
  • Neuropathic pain is a type of pathophysiological chronic pain state, and unlike other types of chronic pain such as nociceptive or inflammatory pain, is maladaptive, therefore neither protects or supports healing or repair (Costigan M, et al., 2009). 

Neuropathic pain

NITROGEN SPECIES AND PAIN

  Nitric oxideHNO

         Nitric Oxide production                               Nitroxyl production:  DOI: 10.1016/j.niox.2016.04.006

Nitric oxide is able to act as a neurotransmitter following neuronal generation and release to signal during neuropathic pain, however it also acts via a neuronal- glial pathway with glial cells also able to produce nitric oxide in large amounts contributing to the development and maintenance of neuropathic pain. 

Nitroxyl, the protonated form of nitric oxide has also been implicated in neuropathic pain signalling, however the endogenous role has yet to be determined. Recently, Longhi-Balbinot et al (2016), demonstrated that administration of HNO donor was able to reduce pain sensitivity (hyperalgesia) by reducing the reactivity of spinal glia and expression of their associated cytokines. 

To be continued......Work in progress..........

 

Appointments

Date Position Institution name
2011 Research Associate University of Adelaide
2003 - 2010 Research Assistant University of Melbourne
1999 - 2003 Research Assistant University of Adelaide

Language Competencies

Language Competency
Greek, Modern (1453-) Can read, write, speak and understand spoken

Education

Date Institution name Country Title
2016 University of Adelaide Australia PhD
2003 - 2004 Monash University Australia Graduate Certificate - Secondary Education
1997 - 2000 University of Adelaide Australia BSc (Honours)

Research Interests

Journals

Year Citation
2018 Beckett, E., Staikopoulos, V., & Hutchinson, M. (2018). Differential effect of morphine on gastrointestinal transit, colonic contractions and nerve-evoked relaxations in Toll-Like Receptor deficient mice. Scientific Reports, 8(1), 5923-1-5923-13.
DOI
2016 Jacobsen, J., Parker, L., Everest-Dass, A., Schartner, E., Tsiminis, G., Staikopoulos, V., . . . Mustafa, S. (2016). Novel imaging tools for investigating the role of immune signalling in the brain. Brain, Behavior, and Immunity, 58, 40-47.
DOI Scopus4 WoS4 Europe PMC1
2016 Wardill, H., Gibson, R., Van Sebille, Y., Secombe, K., Coller, J., White, I., . . . Bowen, J. (2016). Irinotecan-induced gastrointestinal dysfunction and pain are mediated by common TLR4-dependent mechanisms. Molecular Cancer Therapeutics, 15(6), 1376-1386.
DOI Scopus13 WoS12 Europe PMC9
2016 Grace, P., Gaudet, A., Staikopoulos, V., Maier, S., Hutchinson, M., Salvemini, D., & Watkins, L. (2016). Nitroxidative signaling mechanisms in pathological pain. Trends in Neurosciences, 39(12), 862-879.
DOI Scopus13 WoS14 Europe PMC10
2015 Lee, S., Hutchinson, M., Staikopoulos, V., & Saint, D. (2015). Amitriptyline pharmacologically preconditions rat hearts against cardiac ischemic-reperfusion injury. International Journal of Cardiology, 190, 353-359.
DOI Scopus6 WoS5 Europe PMC5
2015 Dodds, K., Staikopoulos, V., & Beckett, E. (2015). Uterine contractility in the nonpregnant mouse: changes during the estrous cycle and effects of chloride channel blockade. Biology of Reproduction, 92(6), 141-1-141-15.
DOI Scopus10 WoS9 Europe PMC9
2013 Spencer, N., Nicholas, S., Sia, T., Staikopoulos, V., Kyloh, M., & Spencer, E. (2013). By what mechanism does ondansetron inhibit colonic migrating motor complexes: does it require endogenous serotonin in the gut wall?. Neurogastroenterology and Motility, 25(8), 677-685.
DOI Scopus16 WoS15 Europe PMC13
2011 Ivanusic, J., Beaini, D., Hatch, R., Staikopoulos, V., Sessle, B., & Jennings, E. (2011). Peripheral N-methyl-ᴅ-aspartate receptors contribute to mechanical hypersensitivity in a rat model of inflammatory temporomandibular joint pain. European Journal of Pain, 15(2), 179-185.
DOI
2009 Cho, H. J., Staikopoulos, V., Furness, J., & Jennings, E. (2009). Inflammation-induced increase in hyperpolarization-activated, cyclic nucleotide-gated channel protein in trigeminal ganglion neurons and the effect of buprenorphine. Neuroscience, 162(2), 453-461.
DOI
2009 Weston, R., Subasinghe, K., Staikopoulos, V., & Jarrott, B. (2009). Design and Assessment of a Potent Sodium Channel Blocking Derivative of Mexiletine for Minimizing Experimental Neuropathic Pain in Several Rat Models. Neurochemical Research, 34(10), 1816-1823.
DOI
2009 Cho, H., Staikopoulos, V., Ivanusic, J., & Jennings, E. (2009). Hyperpolarization-activated cyclic-nucleotide gated 4 (HCN4) protein is expressed in a subset of rat dorsal root and trigeminal ganglion neurons. Cell and Tissue Research, 338(2), 171-177.
DOI
2008 Janovska, A., Hatzinikolas, G., Staikopoulos, V., McInerney, J., Mano, M., & Wittert, G. (2008). AMPK and ACC phosphorylation: Effect of leptin, muscle fibre type and obesity. Molecular and Cellular Endocrinology, 284(1-2), 1-10.
DOI Scopus31 WoS27 Europe PMC19
2008 Albanese, V., Lawson, V., Hill, A., Cappai, R., Di Guardo, G., Staikopoulos, V., . . . Chiocchetti, R. (2008). Evidence for prion protein expression in enteroglial cells of the myenteric plexus of mouse intestine. Autonomic Neuroscience-Basic & Clinical, 140(1-2), 17-23.
DOI
2007 Staikopoulos, V., Sessle, B., Furness, J., & Jennings, E. (2007). Localization of P2X2 and P2X3 receptors in rat trigeminal ganglion neurons. Neuroscience, 144(1), 208-216.
DOI
2005 Hind, A., Migliori, M., Thacker, M., Staikopoulos, V., Nurgali, K., Chiocchetti, R., & Furness, J. (2005). Primary afferent neurons intrinsic to the guinea-pig intestine, like primary afferent neurons of spinal and cranial sensory ganglia, bind the lectin, IB4. Cell and Tissue Research, 321(2), 151-157.
DOI

Conference Papers

Year Citation
2016 Staikopoulos, V., Gosnell, M., Anwer, A., Mustafa, S., Hutchinson, M., & Goldys, E. (2016). Hyperspectral imaging of endogenous fluorescent metabolic molecules to identify pain states in central nervous system tissue. In M. Hutchinson, & E. Goldys (Eds.), Proceedings of SPIE - The International Society for Optical Engineering Vol. 10013 (pp. 1-8). Adelaide, AUSTRALIA: SPIE-INT SOC OPTICAL ENGINEERING.
DOI
2015 Dodds, K., Beckett, E., Staikopoulos, V., Evans, S., & Hutchinson, M. (2015). Novel mouse model of endometriosis permits identification of spinal glia and nociception characteristics involved in pelvic pain. In JOURNAL OF NEUROCHEMISTRY Vol. 134 (pp. 267). Cairns, AUSTRALIA: WILEY-BLACKWELL.
2015 Staikopoulos, V., Jin, D., Lu, Y., Gibson, B., Reineck, P., Goldys, E., . . . Hutchinson, M. (2015). Ultra-structural imaging in cleared mouse cns using nanoparticles. In JOURNAL OF NEUROCHEMISTRY Vol. 134 (pp. 382). Cairns, AUSTRALIA: WILEY-BLACKWELL.
2015 Jacobsen, J., Staikopoulos, V., & Hutchinson, M. (2015). Optimisation and utilisation of a novel whole-brain imaging technique (clarity) to analyse addiction-like behaviours. In JOURNAL OF NEUROCHEMISTRY Vol. 134 (pp. 351). Cairns, AUSTRALIA: WILEY-BLACKWELL.

Work carried out during this PhD is being funded by an Australian Postgraduate Award and the ARC Centre of Excellence for Nanoscale Biophotonics.

APA

cnbp

http://www.cnbp.org.au/  

Committee Memberships

Date Role Committee Institution Country
2016 - ongoing Representative ARC CNBP Executive Memebers Committee University of Adelaide Australia
2016 - ongoing Representative ANS Student Body Australiasian Neuroscience Society Australia

Memberships

Date Role Membership Country
2015 - ongoing Member Society of Neuroscience United States
2015 - ongoing Member Australasian Neuroscience Society Australia
Position
Laboratory Manager - RA
Phone
83134566
Campus
North Terrace
Building
Helen Mayo South, floor S5
Room Number
S5 13A
Org Unit
Faculty of Health and Medical Sciences

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