Tan Dai Nguyen

Tan Dai Nguyen

School of Biological Sciences

Faculty of Sciences, Engineering and Technology

Dr. Tan Dai Nguyen is an ARC-funded researcher affiliated with the Reproductive Success Group (Professor Kylie Dunning) and the Centre of Light for Life (Professor Kishan Dholakia). He also holds appointments with the Robinson Research Institute (RRI), the School of Biomedicine, and the Institute for Photonics and Advanced Sensing (IPAS).His research focuses on developing advanced biomedical devices, including microfluidic and surface acoustic wave (SAW) platforms, for applications in reproductive biology. He aims to design sound wave-based culture systems for the long-term in vitro culture of follicles and oocytes, with the broader goal of advancing reproductive technologies and improving cell culture methodologies.Dr. Nguyen earned his PhD in Mechanical Engineering from Nanyang Technological University under Professor Du Hejun, where he developed acoustic microfluidic systems for manipulating microparticles and cells, laying foundations for tissue engineering and biostructure fabrication.As a postdoctoral researcher in Professor Jongyoon Han’s lab at the Singapore-MIT Alliance for Research and Technology (SMART), he led the development of a label-free, high-throughput microfluidic platform for removing undifferentiated cells from iPSC-derived populations- work that reduced tumorigenicity risk in stem cell products and was featured in MIT News (https://news.mit.edu/2024/scientists-develop-low-cost-device-safer-cell-therapy-0207). These efforts resulted in two international patents.Prior to his current role, Dr. Nguyen was a scientist at A*STAR (Biomanufacturing Technology Group, Singapore), where he worked on scalable technologies for cell therapy manufacturing. His projects included continuous in-line systems for viral inactivation and microfluidic platforms to enhance viral transduction in T-cell therapy. This work supported improvements in process reliability, scalability, and cost-effectiveness, and led to another international patent.

  • Journals
    Year Citation
    2025 Lee, K. Z., Nguyen, T. D., & Liu, D. (2025). Optimizing T cell transduction: a novel transduction device for efficient and scalable gene delivery. Journal of Translational Medicine, 23(1), 899.
    DOI
    2024 Chen, S., Nguyen, T. D., Lee, K. -Z., & Liu, D. (2024). Ex vivo T cell differentiation in adoptive immunotherapy manufacturing: Critical process parameters and analytical technologies. Biotechnology Advances, 77, 108434-1-108434-16.
    DOI Scopus2 WoS2 Europe PMC2
    2024 Lee, J. S. Z., Nguyen, T. D., Zheng, Z. Y., Zhang, W., & Liu, D. (2024). Real‐Time Adaptive Inline Acidification Enhances Continuous pH Control for Viral Inactivation. Biotechnology Journal, 19(11), 10 pages.
    DOI Scopus1 WoS1 Europe PMC1
    2024 Tan, J., Chen, J., Roxby, D., Chooi, W. H., Nguyen, T. D., Ng, S. Y., . . . Chew, S. Y. (2024). Using magnetic resonance relaxometry to evaluate the safety and quality of induced pluripotent stem cell-derived spinal cord progenitor cells. Stem Cell Research & Therapy, 15(1), 15 pages.
    DOI
    2024 Nguyen, T. D., Chooi, W. H., Jeon, H., Chen, J., Tan, J., Roxby, D. N., . . . Han, J. (2024). Label-Free and High-Throughput Removal of Residual Undifferentiated Cells From iPSC-Derived Spinal Cord Progenitor Cells. Stem Cells Translational Medicine, 13(4), 387-398.
    DOI Scopus10 WoS10 Europe PMC9
    2021 Nguyen, T. D., Tran, V. T., & Du, H. (2021). Manipulation of self-assembled three-dimensional architecture in reusable acoustofluidic device. Electrophoresis, 42(21-22), 2375-2382.
    DOI Scopus3 WoS28 Europe PMC1
    2021 Nguyen, T. D., Tran, V. T., Pudasaini, S., Gautam, A., Lee, J. M., Fu, Y. Q., & Du, H. (2021). Large-Scale Fabrication of 3D Scaffold-Based Patterns of Microparticles and Breast Cancer Cells using Reusable Acoustofluidic Device. Advanced Engineering Materials, 23(6), 8 pages.
    DOI Scopus13 WoS15
    2020 Nguyen, T. D., Fu, Y. Q., Tran, V. T., Gautam, A., Pudasaini, S., & Du, H. (2020). Acoustofluidic closed-loop control of microparticles and cells using standing surface acoustic waves. Sensors and Actuators, B: Chemical, 318, 9 pages.
    DOI Scopus38 WoS32
    2019 Tao, X., Nguyen, T. D., Jin, H., Tao, R., Luo, J., Yang, X., . . . Fu, Y. Q. (2019). 3D patterning/manipulating microparticles and yeast cells using ZnO/Si thin film surface acoustic waves. Sensors and Actuators, B: Chemical, 299, 9 pages.
    DOI Scopus40 WoS39
    2018 Nguyen, T. D., Tran, V. T., Fu, Y. Q., & Du, H. (2018). Patterning and manipulating microparticles into a three-dimensional matrix using standing surface acoustic waves. Applied Physics Letters, 112(21), 5 pages.
    DOI Scopus46 WoS44

  • Conference Papers
    Year Citation
    2022 Nguyen, T. D., Chen, J., Liu, Q., Chew, S. Y., & Han, J. (2022). Label-free detection of residual undifferentiated iPSCs from their differentiated progenitor cells by microfluidic raman spectroscopy. In TISSUE ENGINEERING PART A Vol. 28 (pp. 567). SOUTH KOREA, Jeju: MARY ANN LIEBERT, INC.
    2022 Roxby, D., Chen, J., Yao, J. T. Z., Nguyen, T. D., Chew, S. Y., & Han, J. (2022). Magnetic resonance relaxometry as a tool for tracking induced pluripotent stem cell variability. In TISSUE ENGINEERING PART A Vol. 28 (pp. 563). SOUTH KOREA, Jeju: MARY ANN LIEBERT, INC.

  • Preprint
    Year Citation
    2022 Nguyen, T. D., Chooi, W. H., Jeon, H., Chen, J., Roxby, D. N., Zu Yao, J. T., . . . Han, J. (2022). Label-free and high-throughput removal of residual undifferentiated cells from iPSC-derived spinal-cord progenitor cells.
    DOI

Career Development Fund - Pitchfest for Early Career Researchers, Agency for Science, Technology and Research (A*STAR), Singapore, $70,000 SGD

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