Renato Morona

Research Interests

Animal Cell and Molecular Biology Biochemistry & Molecular Biology Cell Biology Infectious Diseases Innate Immunity Medical Infection Agents Medical Microbiology Microbial Genetics Microbiology Microscopy

APrf Renato Morona

School of Biological Sciences

College of Sciences

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

My laboratory studies the human specific bacterial pathogen Shigella flexneri which causes bacillary dysentery. Current research areas are :1) Role of membrane proteins in biosynthesis of LPS O antigen and Enterobacterial Common Antigen2) Function of LPS O antigen in pathogenesis3) Function of IcsA protein in pathogenesis

Invasive Bacterial Pathogens Laboratory

My laboratory conducts research into a number of enteric and invasive bacterial pathogens with a focus on the role of bacterial cell surface molecules in bacterial pathogenesis.

My research interests are:

Shigella flexneri

Actin-based motility

Lipopolysaccharides  and O Antigens

Regulation of Capsule Polysaccharides

Outer Membrane Proteins

Bacteriophage

Receptor Mimic Technology

Anti-microbial drug discovery

PhD and Honours Projects

A number of challenging projects are available to interested PhD and Honours students. These projects will be in the general area of Shigella flexneri virulence factors, with an emphasis on the structure and function of IcsA, on lipopolysaccharide (LPS) O antigen biosynthesis proteins, and bacterial cell biology. Project information is available in the Honours and PhD page of the School website.

Other Pathogens we have Studied Include:

Salmonella enterica Typhi

Salmonella enterica Typhimurium

Vibrio cholerae O1

Shigella flexneri

Shigella flexneri infected HeLa cellsImage removed.

Shigella flexneri are Gram-negative bacteria that cause bacillary dysentery (shigellosis) in humans, and result in over 100 million episodes of diarrhea and 1000's of deaths each year.

Shigellosis arises due to poor sanitation and hygiene, and is transmitted by the oral-faecal route. Antibiotic therapy is frequently ineffective due to multi-antibiotic resistance. Shigellae are facultative intracellular pathogens able to invade, replicate within, and kill intestinal cells, and can also spread from cell to cell without going through an extracellular stage.

The infection results in an acute inflammatory response in the colon that causes the majority of the disease symptoms. S. flexneri bacteria are able to invade intestinal cells and replicate within them. After escaping into the cytosol, they replicate and exhibit intracellular motility in which the polarly localized cell surface protein IcsA recruits host proteins to generate actin-based motility (ABM). ABM allows the bacteria to spread from cell to cell thereby creating a focus of infection.

Relevant Publications

Lum, M., and Morona R. (2014) Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection. Int J Med Microbiol. 2014 Apr 3. pii: S1438-4221(14)00027-7. doi: 10.1016/j.ijmm.2014.03.006. [Epub ahead of print]

Lum, M., and Morona R. (2014) Myosin IIA is essential for Shigella flexneri cell-to-cell spread. Pathog Dis. doi: 10.1111/2049-632X.12202.

Lum, M., Attridge, S.R., and Morona R. (2013) Impact of dynasore an inhibitor of dynamin II on Shigella flexneri Infection. PLoS One. 8(12):e84975. doi: 10.1371/journal.pone.0084975. eCollection 2013.

Actin Based Motility

Actin based motility (ABM) is used by a number of microbial pathogens to move inside and between infected host (animal and human) cells. In Shigella flexneri, the outer membrane IcsA protein is reponsible for nucleating actin polymerisation by subverting host cell actin and actin regulatory proteins (e.g. N-WASP, Arp2/3 complex, Profilin, Cofilin, CapZ).

ABM can be visualized by staining using FITC-phalloidin to detect the F-actin comet tails that are formed. Additionally, time-lapse video microscopy can be used to visualise and measure the rate of motility.

In addition to IcsA, bacterial factors such as lipopolysaccharides also impact on IcsA function and intercellular spreading.

Relevant Publications

Morona, R., and Van Den Bosch, L. (2003) "Lipopolysaccharide O antigen chains mask IcsA(VirG) in Shigella flexneri" FEMS Microbiol. Lett. 221: 173-180.

Morona, R., and Van Den Bosch, L. (2003) "Multi-copy icsA is able to suppress the virulence defect caused by the wzzSF mutation in Shigella flexneri." FEMS Microbiol. Lett. 221: 213-219.

Van Den Bosch, L. and Morona, R. (2003) "The actin-based motility defect of Shigella flexneri rmlD rough LPS mutant is not due to loss of IcsA polarity." Microb. Pathogen. 35: 11-18.

Van Den Bosch, L., Manning, P.A., and Morona, R. (1997) “Regulation of O-antigen chain length is required for Shigella flexneri virulence”. Mol. Microbiol. 23: 765-775

Lipopolysaccharides

Image removed.

Lipopolysaccharide (LPS) molecules are located in the outer leaflet of the outer membrane of bacteria such as Shigella flexneri and Salmonella enterica Typhimurium. LPS molecules are amphipathic and have three regions: lipid A, core sugars, and O antigen polysaccharides. The O antigen polysaccharides of S. flexneri and S. Typhimurium are composed of repeats of a tetrasaccharide unit, and range from 1 to >100 repeat units in length. The length of O antigen polysaccharides is regulated by the activity of Wzz proteins, with different Wzz proteins able to confer different modal length distributions. O antigen polysaccharide chain length impacts on virulence as it affects resistance to Complement, and actin based motility.

Relevant Publications

Kalynych, S., Morona, R., and Miroslaw, C. (2014) Progress in understanding the assembly process of bacterial O-Antigen. FEMS Microbiol Rev. 2014 Mar 11. doi: 10.1111/1574-6976.12070. [Epub ahead of print]

Tran, E.N.H. and Morona R. (2013) Residues located inside the Escherichia coli FepE protein oligomer are essential for lipopolysaccharide O-antigen modal chain length regulation. Microbiology (SGM). 159: 701-714.

Papadopoulos, M and Morona, R. (2010) Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. J. Bacteriol. 192: 3385-3393.

Morona, R., Purins, L., Tocilj, A., Matte, A., and Cygler M. (2009) Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins. Trends Biochem Sci. 34:78-84.

Purins, L., Van Den Bosch, L., and Richardson, V., and Morona, R. (2008) Coiled-coil regions play a role in the function of the Shigella flexneri O-antigen chain length regulator WzzpHS2. Microbiology (SGM) 154:1104-1116.

Tocilj, A., Munger, C., Proteau, A., Morona, R., Purins, L., Ajamina, E., Wagner, J., Papadopoulos, M., Van Den Bosch, L., Rubinstein, J.L., Féthière, J., Matte, A., and Cygler, M. (2008) Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Nature Struct. Mol. Biol. 15:130-138.

Murray, G.L., Attridge, S.R., and Morona, R. (2006) Altering the length of Salmonella typhimurium lipopolysaccharide O antigen has an impact on interaction with macrophages and complement". J. Bacteriol. 188: 2735-2739.

Murray, G.L., Attridge, S.R., and Morona, R. (2005) "Inducible serum resistance in Salmonella typhimurium is dependent on wzz(fepE)-regulated very long O antigen chains". Microbes Infect. 7: 1296-1304.

Morona, R., Daniels, C., and Van Den Bosch, L. (2003) “Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimised for virulence.” Microbiology, 49: 925-939.

Murray, G.L., Attridge, S.R., and Morona, R. (2003) “Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz.” Mol. Microbiol. 47: 1395-1406.

Morona, R., and Van Den Bosch, L. (2003) "Lipopolysaccharide O antigen chains mask IcsA(VirG) in Shigella flexneri" FEMS Microbiol. Lett. 221: 173-180.

Morona, R., Van Den Bosch, L., and Daniels, C. (2000) “Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions.”  Microbiol. 146: 1-4.

Table for this paper.

Baker, S.J., Gunn, J.S. and Morona, R. (1999) “The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance, and lipopolysaccharide.” Microbiol. 145: 367-378.

Daniels, C., and Morona, R. (1999) Analysis of Shigella flexneri Wzz (Rol) function by mutagenesis and cross-linking: Wzz is able to oligomerise. Mol. Microbiol. 34: 181-194.

Daniels, C., Vindurampulle, C., and Morona, R. (1998) Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Mol. Microbiol. 28: 1211-1222.

Van Den Bosch, L., Manning, P.A., and Morona, R. (1997) “Regulation of O-antigen chain length is required for Shigella flexneri virulence”. Mol. Microbiol. 23: 765-775.

Outer Membrane Proteins

The outer membrane (OM) of Gram-negative bacteria is in direct contact with the external environment, and contains proteins, lipoproteins, phospholipids, and lipopolysaccharides (LPSs). OM proteins (OMPs) are often directly involved in the virulence of pathogenic bacteria. The Shigella flexneri OMP called IcsA protein is essential for bacterial motility inside infected human cells, while another S. flexneri OMP is IcsP which is a protease that cleaves IcsA.

IcsA and IcsP

The IcsA protein is a large 116 kDa outer membrane protein which is able to interact with actin regulatory proteins within the cytosol of host (human and animal) cells to trigger polymerisation of actin and hence actin-based motility. IcsA is a member of the autotransporter family of proteins, and has an 85 kDa amino terminal passenger (or alpha domain) and a 37 kDa carboxy terminal transporter (or beta domain) that is located in the outer membrane. The IcsA protein is it is targeted to and predominantly localised at the old cell pole in strains that have intact LPS. Polar localisation is relaxed in strains unable to produce either LPS O antigens or the IcsP protease.

Relevant Publications

Zumsteg, A.B., Goosmann, C., Brinkmann, V., Morona, R., and Arturo Zychlinsky, A. (2014) IcsA is an adhesin in Shigella flexneri that is regulated by the activity of the Type III secretion system. Cell Host and Microbe 15:435-445.

Tran, E.N, Doyle, M.T, and Morona R. (2013) LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles. PLoS One. 2013 Jul 25;8(7):e70508. doi: 10.1371/journal.pone.0070508.

Teh, M.Y. and Morona, R. (2013) Identification of Shigella flexneri IcsA residues affecting interaction with N-WASP, and evidence for IcsA-IcsA co-operative interaction. PLoS One. 2013; 8(2):e55152. doi: 10.1371/journal.pone.0055152. Epub 2013 Feb 6.

Teh, M.Y., Tran, E.N., and Morona, R. (2012) Absence of O-antigen suppresses Shigella flexneri IcsA autochaperone region mutations. Microbiology (SGM). 158: 2835-2850.

May, K.L., Grabowicz, M., Polyak, S.W. and Morona, R. (2012) Self-association of the Shigella flexneri IcsA autotransporter. Microbiology (SGM). 158:1874-1883.

Lum, M. and Morona, R. (2012) IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface. Microbial Cell Factories. 11:20. doi: 10.1186/1475-2859-11-20.

May, K.M. and Morona, R. (2008) Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. J Bacteriol. 190:4666-4676.

 

 

 

Date Institution name Country Title
1982 University of Adelaide Australia PhD
1978 University of Adelaide Australia B.Sc. (Hons)
1977 University of Adelaide Australia B.Sc.

Year Citation
2025 Qin, J., Hong, Y., Vollmer, W., Morona, R., & Totsika, M. (2025). Disruptions in outer membrane-peptidoglycan interactions enhance bile salt resistance in O-antigen-producing E. coli. mBio, 16(10), e0218425-1-e0218425-20.
DOI WoS1
2025 Qin, J., Hong, Y., Maczuga, N. T., Morona, R., & Totsika, M. (2025). Tolerance mechanisms in polysaccharide biosynthesis: Implications for undecaprenol phosphate recycling in Escherichia coli and Shigella flexneri.. PLoS genetics, 21(1), e1011591.
DOI Scopus2 WoS3 Europe PMC3
2025 Ascari, A., & Morona, R. (2025). Recent insights into Wzy polymerases and lipopolysaccharide O-antigen biosynthesis. Journal of Bacteriology, 207(4), 17 pages.
DOI Scopus3 WoS3 Europe PMC4
2023 Ascari, A., Waters, J. K., Morona, R., & Eijkelkamp, B. A. (2023). Shigella flexneri Adapts to Niche-Specific Stresses through Modifications in Cell Envelope Composition and Decoration. ACS Infectious Diseases, 9(8), 1610-1621.
DOI Scopus7 WoS7 Europe PMC7
2023 Qin, J., Hong, Y., Morona, R., & Totsika, M. (2023). O antigen biogenesis sensitises Escherichia coli K-12 to bile salts, providing a plausible explanation for its evolutionary loss. PLoS Genetics, 19(10), e1010996-1-e1010996-21.
DOI Scopus8 WoS9 Europe PMC13
2023 Ascari, A., Frölich, S., Zang, M., Tran, E. N. H., Wilson, D. W., Morona, R., & Eijkelkamp, B. A. (2023). Shigella flexneri remodeling and consumption of host lipids during infection. Journal of Bacteriology, 205(12), 1-18.
DOI Scopus3 WoS3 Europe PMC3
2022 Ascari, A., Tran, E. N. H., Eijkelkamp, B. A., & Morona, R. (2022). Detection of a disulphide bond and conformational changes in Shigella flexneri Wzy, and the role of cysteine residues in polymerase activity. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1864(5), 183871-1-183871-12.
DOI Scopus8 WoS7 Europe PMC7
2022 Teh, M. Y., Tran, E. N. H., & Morona, R. (2022). Bacteriophage Sf6 host range mutant that infects Shigella flexneri serotype 2a₂ strains. FEMS Microbiology Letters, 369(1), 1-5.
DOI Scopus3 WoS2 Europe PMC2
2022 Maczuga, N., Tran, E. N. H., Qin, J., & Morona, R. (2022). Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways. Journal of Bacteriology, 204(4), 1-16.
DOI Scopus16 WoS16 Europe PMC19
2022 Maczuga, N. T., Tran, E. N. H., & Morona, R. (2022). Topology of the Shigella flexneri Enterobacterial Common Antigen polymerase WzyE. Microbiology, 168(4), 001183-1-001183-11.
DOI Scopus5 WoS5 Europe PMC6
2022 Qin, J., Hong, Y., Pullela, K., Morona, R., Henderson, I. R., & Totsika, M. (2022). A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide. Scientific Reports, 12(1), 11629-1-11629-8.
DOI Scopus14 WoS15 Europe PMC18
2022 Qin, J., Hong, Y., Morona, R., & Totsika, M. (2022). Cysteine dependent conformation heterogeneity of <i>Shigella flexneri</i> autotransporter IcsA and implications in its function.
DOI
2022 Ascari, A., Tran, E. N. H., Eijkelkamp, B. A., & Morona, R. (2022). Identification of the Shigella flexneri Wzy Domain Modulating
WzzpHS-2 Interaction and Detection of the Wzy/Wzz/Oag
Complex. Journal of Bacteriology, 204(9), e0022422-1-e0022422-18.
DOI Scopus10 WoS10 Europe PMC12
2022 Maczuga, N., Tran, E. N. H., & Morona, R. (2022). Subcellular localization of the enterobacterial common antigen GT-E-like glycosyltransferase, WecG. Molecular Microbiology, 118(4), 403-416.
DOI Scopus3 WoS3 Europe PMC4
2022 Qin, J., Hong, Y., Morona, R., & Totsika, M. (2022). Cysteine-Dependent Conformational Heterogeneity of Shigella flexneri Autotransporter IcsA and Implications of Its Function. Microbiology Spectrum, 10(6), 1-16.
DOI Scopus4 WoS4 Europe PMC6
2021 Doore, S. M., Subramanian, S., Tefft, N. M., Morona, R., TerAvest, M. A., & Parent, K. N. (2021). Large metabolic rewiring from small genomic changes between strains of Shigella flexneri. Journal of Bacteriology, 203(11), 1-13.
DOI Scopus9 WoS8 Europe PMC8
2021 Leo, V., Teh, M. Y., Tran, N., & Morona, R. (2021). Identification of a region in Shigella flexneri WzyB disrupting the interaction with WzzpHS2. Journal of Bacteriology, 203(22), e00413-21-1-e00413-21-15.
DOI Scopus6 WoS5 Europe PMC7
2020 Leo, V., Tran, N., & Morona, R. (2020). Polysaccharide co-polymerase WzzB/WzzE chimeras reveal transmembrane 2 region of WzzB is important for interaction with WzyB. Journal of Bacteriology, 203(6), 1-14.
DOI Scopus15 WoS12 Europe PMC15
2020 Tran, N. H., Day, C. J., Poole, J., Jennings, M. P., & Morona, R. (2020). Specific blood group antibodies inhibit Shigella flexneri interaction with human cells in the absence of spinoculation. Biochemical and Biophysical Research Communications, 521(1), 131-136.
DOI Scopus5 WoS4 Europe PMC4
2020 Qin, J., Doyle, M. T., Tran, E. N. H., & Morona, R. (2020). The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function.. PLoS One, 15(1), e0227425-1-e0227425-19.
DOI Scopus15 WoS15 Europe PMC16
2020 Tran, E. N. H., Day, C. J., McCartney, E., Poole, J., Tse, E., Jennings, M. P., & Morona, R. (2020). Shigella flexneri targets human colonic goblet cells by O antigen binding to Sialyl-Tn and Tn antigens via glycan-glycan interactions. ACS Infectious Diseases, 6(10), 2604-2615.
DOI Scopus11 WoS11 Europe PMC11
2020 Teh, M. Y., Furevi, A., Widmalm, G., & Morona, R. (2020). Influence of Shigella flexneri 2a O antigen acetylation on its bacteriophage Sf6 receptor activity, and bacterial interaction with human cells. Journal of Bacteriology, 202(24), 1-19.
DOI Scopus10 WoS11 Europe PMC12
2019 Helbig, K. J., Teh, M. Y., Crosse, K. M., Monson, E. A., Smith, M., Tran, E. N., . . . Beard, M. R. (2019). The interferon stimulated gene viperin, restricts Shigella. flexneri in vitro.. Sci Rep, 9(1), 15598.
DOI Scopus20 WoS19 Europe PMC18
2018 Ahmad, Z., Harvey, R., Paton, J., Standish, A., & Morona, R. (2018). Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva. PLoS ONE, 13(1), 1-15.
DOI Scopus3 WoS3 Europe PMC3
2018 Ahmad, Z., Morona, R., & Standish, A. (2018). In vitro characterization and identification of potential substrates of a low molecular weight protein tyrosine phosphatase in Streptococcus pneumoniae. Microbiology, 164(4), 697-703.
DOI Scopus3 WoS3 Europe PMC4
2016 Standish, A., Teh, M., Tran, E., Doyle, M., Baker, P., & Morona, R. (2016). Unprecedented abundance of protein tyrosine phosphorylation modulates Shigella flexneri virulence. Journal of Molecular Biology, 428(20), 4197-4208.
DOI Scopus21 WoS21 Europe PMC21
2016 Papadopoulos, M., Tran, E., Murray, G., & Morona, R. (2016). Conserved transmembrane glycine residues in the shigella flexneri polysaccharide co-polymerase protein WzzB influence protein-protein interaction. Microbiology, 162(6), 921-929.
DOI Scopus6 WoS5 Europe PMC7
2015 Doyle, M., Grabowicz, M., May, K., & Morona, R. (2015). Lipopolysaccharide surface structure does not influence IcsA polarity. FEMS Microbiology Letters, 362(8), fnv042-1-fnv042-7.
DOI Scopus2 WoS2 Europe PMC2
2015 Doyle, M., Tran, E., & Morona, R. (2015). The passenger-associated transport repeat promotes virulence factor secretion efficiency and delineates a distinct autotransporter subtype. Molecular microbiology, 97(2), 315-329.
DOI Scopus11 WoS8 Europe PMC9
2015 Tran, E., Attridge, S., Teh, M., & Morona, R. (2015). Shigella flexneri cell-to-cell spread, and growth and inflammation in mice, is limited by the outer membrane protease IcsP. FEMS Microbiology Letters, 362(12), fnv088-1-fnv088-8.
DOI Scopus1 WoS1 Europe PMC1
2015 Paton, A., Chen, A., Wang, H., McAllister, L., Höggerl, F., Mayr, U., . . . Paton, J. (2015). Protection against Shiga-toxigenic Escherichia coli by non-genetically modified organism receptor mimic bacterial ghosts. Infection and Immunity, 83(9), 3526-3533.
DOI Scopus9 WoS13 Europe PMC5
2015 Nath, P., & Morona, R. (2015). Detection of Wzy/Wzz interaction in Shigella flexneri. Microbiology, 161(9), 1797-1805.
DOI Scopus29 WoS25 Europe PMC27
2015 Doyle, M., Grabowicz, M., & Morona, R. (2015). A small conserved motif supports polarity augmentation of Shigella flexneri IcsA. Microbiology, 161(11), 2087-2097.
DOI Scopus7 WoS7 Europe PMC8
2015 Chang, C., Tran, E., Ericsson, D., Casey, L., Lonhienne, T., Benning, F., . . . Kobe, B. (2015). Structural and biochemical analysis of a single amino-acid mutant of WzzBSF that alters lipopolysaccharide O-antigen chain length in shigella flexneri. PLoS One, 10(9), e0138266-1-e0138266-21.
DOI Scopus13 WoS11 Europe PMC7
2015 Day, C., Tran, E., Semchenko, E., Tram, G., Hartley-Tassell, L., Ng, P., . . . Jennings, M. (2015). Glycan: glycan interactions: high affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells. Proceedings of the National Academy of Sciences of the United States of America, 112(52), E7266-E7275.
DOI Scopus107 WoS103 Europe PMC83
2015 Whittall, J., Morona, R., & Standish, A. (2015). Topology of Streptococcus pneumoniae CpsC, a Polysaccharide Copolymerase and Bacterial Protein Tyrosine Kinase Adaptor Protein. Journal of Bacteriology, 197(1), 120-127.
DOI Scopus7 WoS7 Europe PMC7
2015 Nath, P., Tran, E., & Morona, R. (2015). Mutational analysis of the Shigella flexneri O-antigen polymerase Wzy: Identification of Wzz-dependent Wzy mutants. Journal of Bacteriology, 197(1), 108-119.
DOI Scopus17 WoS16 Europe PMC18
2015 Nath, P., & Morona, R. (2015). Mutational analysis of the major periplasmic loops of Shigella flexneri Wzy; identification of the residues affecting O antigen modal chain length control, and Wzz dependent polymerisation activity. Microbiology, 161(4), 774-785.
DOI Scopus19 WoS17 Europe PMC15
2014 Standish, A., Whittall, J., & Morona, R. (2014). Tyrosine phosphorylation enhances activity of pneumococcal autolysin LytA. Microbiology, 160(12), 2745-2754.
DOI Scopus15 WoS13 Europe PMC14
2014 Lum, M., & Morona, R. (2014). Myosin IIA is essential for Shigella flexneri cell-to-cell spread. Pathogens and Disease, 72(3), 174-187.
DOI Scopus16 WoS15 Europe PMC17
2014 Lum, M., & Morona, R. (2014). Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection. International Journal of Medical Microbiology, 304(5-6), 530-541.
DOI Scopus34 WoS31 Europe PMC31
2014 Zumsteg, A., Goosmann, C., Brinkmann, V., Morona, R., & Zychlinsky, A. (2014). IcsA is a Shigella flexneri adhesin regulated by the type III secretion system and required for pathogenesis. Cell Host and Microbe, 15(4), 435-445.
DOI Scopus80 WoS48 Europe PMC67
2014 Kalynych, S., Morona, R., & Cygler, M. (2014). Progress in understanding the assembly process of bacterial O-antigen. FEMS Microbiology Reviews, 38(5), 1048-1065.
DOI Scopus100 WoS99 Europe PMC102
2014 Tran, E. N. H., Doyle, M. T., & Morona, R. (2014). Correction: LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles (PLoS ONE (2013) 8, 7 (e70508) DOI: 10.1371/journal.pone.0070508). Plos One, 9(10), 5 pages.
DOI
2014 Standish, A., & Morona, R. (2014). The role of bacterial protein tyrosine phosphatases in the regulation of the biosynthesis of secreted polysaccharides. Antioxidants & Redox Signaling, 20(14), 2274-2289.
DOI Scopus39 WoS34 Europe PMC30
2014 Tran, N., Papadopoulos, M., & Morona, R. (2014). Relationship between O-antigen chain length and resistance to colicin E2 in Shigella flexneri. Microbiology, 160(3), 589-601.
DOI Scopus30 WoS28 Europe PMC30
2013 Lum, M., Attridge, S., & Morona, R. (2013). Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. PLoS One, 8(12), e84975-1-e84975-17.
DOI Scopus14 WoS13 Europe PMC13
2013 George, D., Stephenson, D., Tran, E., Morona, R., & Verma, N. (2013). Complete genome sequence of SfII, a serotype-converting bacteriophage of the highly prevalent Shigella flexneri serotype 2a. Genome Announcements, 1(5), e00626-13.
DOI Scopus7 WoS6 Europe PMC7
2013 Morona, R. (2013). Encapsulating bacteria. Structure, 21(5), 692-693.
DOI Scopus1 WoS1 Europe PMC1
2013 Tran, N., Doyle, M., & Morona, R. (2013). LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles. PLoS One, 8(7), 1-16.
DOI Scopus16 WoS17 Europe PMC14
2013 Tran, N., & Morona, R. (2013). Residues located inside the Escherichia coli FepE protein oligomer are essential for lipopolysaccharide O-antigen modal chain length regulation. Microbiology, 159(4), 701-714.
DOI Scopus18 WoS17 Europe PMC18
2013 Teh, M., & Morona, R. (2013). Identification of Shigella flexneri IcsA residues affecting interaction with N-WASP, and evidence for IcsA-IcsA co-operative interaction. PLoS One, 8(2), 1-17.
DOI Scopus10 WoS11 Europe PMC9
2013 Standish, A., Salim, A., Capon, R., & Morona, R. (2013). Dual inhibition of DNA polymerase PolC and protein tyrosine phosphatase CpsB uncovers a novel antibiotic target. Biochemical and Biophysical Research Communications, 430(1), 167-172.
DOI Scopus13 WoS12 Europe PMC12
2012 Teh, M., Tran, N., & Morona, R. (2012). Absence of O antigen suppresses Shigella flexneri IcsA autochaperone region mutations. Microbiology, 158(11), 2835-2850.
DOI Scopus12 WoS13 Europe PMC12
2012 Watts, R., Tan, C., Ulett, G., Carey, A., Totsika, M., Idris, A., . . . Schembri, M. (2012). Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli. Journal of Infectious Diseases, 206(8), 1242-1249.
DOI Scopus26 WoS20 Europe PMC19
2012 Ericsson, D., Standish, A., Kobe, B., & Morona, R. (2012). Wzy-dependent bacterial capsules as potential drug targets. Current Drug Targets, 13(11), 1421-1431.
DOI Scopus14 WoS12 Europe PMC10
2012 May, K., Grabowicz, M., Polyak, S., & Morona, R. (2012). Self-association of the Shigella flexneri IcsA autotransporter protein. Microbiology, 158(7), 1874-1883.
DOI Scopus7 WoS8 Europe PMC8
2012 Paton, A., Morona, R., & Paton, J. (2012). Bioengineered microbes in disease therapy. Trends in Molecular Medicine, 18(7), 417-425.
DOI Scopus41 WoS32 Europe PMC31
2012 Standish, A., Salim, A., Zhang, H., Capon, R., & Morona, R. (2012). Chemical inhibition of bacterial protein tyrosine phosphatase suppresses capsule production. PLoS One, 7(5), 1-8.
DOI Scopus30 WoS27 Europe PMC22
2012 Soares da Costa, T., Tieu, W., Yap, M., Pendini, N., Polyak, S., Pedersen, D., . . . Abell, A. (2012). Selective inhibition of biotin protein ligase from Staphylococcus aureus. Journal of Biological Chemistry, 287(21), 17823-17832.
DOI Scopus63 WoS56 Europe PMC42
2012 Zvarec, O., Polyak, S., Tieu, W., Kuan, K., Dai, H., Pedersen, D., . . . Abell, A. (2012). 5-Benzylidenerhodanine and 5-benzylidene-2-4-thiazolidinedione based antibacterials. Bioorganic & Medicinal Chemistry Letters, 22(8), 2720-2722.
DOI Scopus42 WoS34 Europe PMC17
2012 Lum, M., & Morona, R. (2012). IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface. Microbial Cell Factories, 11(1), 1-10.
DOI Scopus16 WoS15 Europe PMC14
2011 Byrne, J., Morona, J., Paton, J., & Morona, R. (2011). Identification of Streptococcus pneumoniae Cps2C residues that affect capsular polysaccharide polymerization, cell wall ligation, and Cps2D phosphorylation. Journal of Bacteriology, 193(9), 2341-2346.
DOI Scopus18 WoS14 Europe PMC13
2010 Paton, A., Morona, R., & Paton, J. (2010). Bioengineered bugs expressing oligosaccharide receptor mimics: Toxin-binding probiotics for treatment and prevention of enteric infections. Bioengineered Bugs, 1(3), 172-177.
DOI Scopus27 Europe PMC17
2010 Papadopoulos, M., & Morona, R. (2010). Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. Journal of Bacteriology, 192(13), 3385-3393.
DOI Scopus31 WoS30 Europe PMC26
2010 Paton, A., Morona, R., & Paton, J. (2010). Receptor-mimic probiotics: Potential therapeutics for bacterial toxin-mediated enteric diseases. Expert Review of Gastroenterology and Hepatology, 4(3), 253-255.
DOI Scopus4 WoS6 Europe PMC1
2009 Morona, R., Purins, L., Tocilj, A., Matte, A., & Cygler, M. (2009). Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins. Trends in Biochemical Sciences, 34(2), 78-84.
DOI Scopus87 WoS74 Europe PMC67
2009 Attridge, S., Wallerstrom, G., Li, B., Morona, R., & Holmgren, J. (2009). Differential immunogenicity of Vibrio cholerae O139 variants expressing different combinations of naturally occurring and atypical forms of the serogroup polysaccharide. Vaccine, 27(7), 1055-1061.
DOI Scopus4 WoS4 Europe PMC4
2008 May, K., & Morona, R. (2008). Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. Journal of Bacteriology, 190(13), 4666-4676.
DOI Scopus40 WoS41 Europe PMC35
2008 Purins, L., Van Den Bosch, L., Richardson, V., & Morona, R. (2008). Coiled-coil regions play 4 role in the function of the Shigella flexneri O-antigen chain length regulator Wzz(pHS2). Microbiology, 154(Part 4), 1104-1116.
DOI Scopus35 WoS35 Europe PMC32
2008 Tocilj, A., Munger, C., Proteau, A., Morona, R., Purins, L., Ajamian, E., . . . Cygler, M. (2008). Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Nature Structural and Molecular Biology, 15(2), 130-138.
DOI Scopus108 WoS103 Europe PMC95
2006 Paton, A., Morona, R., & Paton, J. (2006). Treatment and Prevention of Enteric Infections With Toxin-binding Probiotics. Discovery Medicine, 6(31), 35-39.
Scopus1
2006 Morona, J., Morona, R., & Paton, J. (2006). Attachment of capsular polysaccharide to the cell wall of Streptococcus pneumoniae type 2 is required for invasive disease. Proceedings of the National Academy of Sciences of the United States of America, 103(22), 8505-8510.
DOI Scopus114 WoS102 Europe PMC92
2006 Focareta, A., Paton, J., Morona, R., Cook, J., & Paton, A. (2006). A recombinant probiotic for treatment and prevention of cholera. Gastroenterology, 130(6), 1688-1695.
DOI Scopus86 WoS74 Europe PMC67
2006 Diaz, P., Slakeski, N., Reynolds, E., Morona, R., Rogers, A., & Kolenbrander, P. (2006). Role of oxyR in the oral anaerobe Porphyromonas gingivalis. Journal of Bacteriology, 188(7), 2454-2462.
DOI Scopus77 WoS79 Europe PMC72
2006 Murray, G., Attridge, S., & Morona, R. (2006). Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar typhimurium with macrophages and complement. Journal of Bacteriology, 188(7), 2735-2739.
DOI Scopus158 WoS149 Europe PMC138
2006 Paton, A., Morona, R., & Paton, J. (2006). Designer probiotics for prevention of enteric infections. Nature Reviews. Microbiology, 4(3), 193-200.
DOI Scopus101 WoS85 Europe PMC59
2005 Paton, A., Jennings, M., Morona, R., Wang, H., Focareta, A., Roddam, L., & Paton, J. (2005). Recombinant probiotics for treatment and prevention of enterotoxigenic escherichia coli diarrhea. Gastroenterology, 128(5), 1219-1228.
DOI Scopus92 WoS79 Europe PMC69
2005 Korres, H., Mavris, M., Morona, R., Manning, P., & Verma, N. (2005). Topological analysis of GtrA and GtrB proteins encoded by the serotype-converting cassette of Shigella flexneri. Biochemical and Biophysical Research Communications, 328(4), 1252-1260.
DOI Scopus32 WoS32 Europe PMC28
2005 Murray, G., Attridge, S., & Morona, R. (2005). Inducible serum resistance in Salmonella typhimurium is dependent on wzzfepE-regulated very long O antigen chains. Microbes and Infection, 7(13), 1296-1304.
DOI Scopus60 WoS58 Europe PMC55
2004 Morona, J., Miller, D., Morona, R., & Paton, J. (2004). The effect that mutations in the conserved capsular polysaccharide biosynthesis genes cpsA, cpsB, and cpsD have on virulence of Streptococcus pneumoniae. Journal of Infectious Diseases, 189(10), 1905-1913.
DOI Scopus113 WoS107 Europe PMC100
2004 Casjens, S., Winn-Stapley, D., Gilcrease, E., Morona, R., Kuhlewein, C., Chua, J., . . . Clark, A. (2004). The chromosome of Shigella flexneri bacteriophage Sf6: Complete nucleotide sequence, genetic mosaicism, and DNA packaging. Journal of Molecular Biology, 339(2), 379-394.
DOI Scopus116 WoS116 Europe PMC116
2004 Pinyon, R., Paton, J., Paton, A., Botten, J., & Morona, R. (2004). Refinement of a therapeutic Shiga toxin-binding probiotic for human trials. Journal of Infectious Diseases, 189(9), 1547-1555.
DOI Scopus37 WoS28 Europe PMC22
2004 CASJENS, S. (2004). The Chromosome of Shigella flexneri Bacteriophage Sf6: Complete Nucleotide Sequence, Genetic Mosaicism, and DNA Packaging*1. Journal of Molecular Biology, 339(2), 379-394.
DOI
2003 Morona, R., & Van Den Bosch, L. (2003). Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri. FEMS Microbiology Letters, 221(2), 173-180.
DOI Scopus32 WoS32 Europe PMC31
2003 Freiberg, A., Morona, R., Van Den Bosch, L., Jung, C., Behlke, J., Carlin, N., . . . Baxa, U. (2003). The tailspike protein of Shigella phage Sf6. A structural homolog of Salmonella phage P22 tailspike protein without sequence similarity in the β-helix domain. Journal of Biological Chemistry, 278(3), 1542-1548.
DOI Scopus48 WoS48 Europe PMC44
2003 Murray, G., Attridge, S., & Morona, R. (2003). Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz. Molecular Microbiology, 47(5), 1395-1406.
DOI Scopus193 WoS187 Europe PMC172
2003 Morona, R., Daniels, C., & Van Den Bosch, L. (2003). Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimized for virulence. Microbiology, 149(4), 925-939.
DOI Scopus99 WoS99 Europe PMC95
2003 Morona, R., & Van Den Bosch, L. (2003). Multicopy icsA is able to suppress the virulence defect caused by wzzSF mutation in the Shigella flexneri. FEMS Microbiology Letters, 221(2), 213-219.
DOI Scopus11 WoS11 Europe PMC9
2003 Morona, J., Morona, R., Miller, D., & Paton, J. (2003). Mutational analysis of the carboxy-terminal (YGX)4 repeat domain of CpsD, an autophosphorylating tyrosine kinase required for capsule biosynthesis in Streptococcus pneumoniae. Journal of Bacteriology, 185(10), 3009-3019.
DOI Scopus82 WoS73 Europe PMC65
2003 Van Den Bosch, L., & Morona, R. (2003). The actin-based motility defect of a Shigella flexneri rmlD rough LPS mutant is not due to loss of IcsA polarity. Microbial Pathogenesis, 35(1), 11-18.
DOI Scopus25 WoS25 Europe PMC25
2002 Morona, J., Morona, R., Miller, D., & Paton, J. (2002). Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel manganese-dependent phosphotyrosine-protein phosphatase. Journal of Bacteriology, 184(2), 577-583.
DOI Scopus112 WoS100 Europe PMC90
2002 Ogunniyi, A., Kotlarski, I., Morona, R., & Manning, P. (2002). Epitope analysis of the FanC subunit protein of the K99 (F5) fimbriae of enterotoxigenic Escherichia coli using a recombinant fusion technique. FEMS Immunology and Medical Microbiology, 34(1), 23-31.
DOI Scopus8 WoS7 Europe PMC6
2001 Paton, J., Rogers, T., Morona, R., & Paton, A. (2001). Oral administration of formaldehyde-killed recombinant bacteria expressing a mimic of the shiga toxin receptor protects mice from fatal challenge with shiga-toxigenic Escherichia coli. Infection and Immunity, 69(3), 1389-1393.
DOI Scopus46 WoS40 Europe PMC31
2001 Paton, A., Morona, R., & Paton, J. (2001). Neutralization of shiga toxins Stx1, Stx2c, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. Infection and Immunity, 69(3), 1967-1970.
DOI Scopus64 WoS56 Europe PMC49
2000 Morona, R., Van Den Bosch, L., & Daniels, C. (2000). Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions. Microbiology, 146(1), 1-3.
DOI Scopus101 WoS89 Europe PMC76
2000 Morona, J., Paton, J., Miller, D., & Morona, R. (2000). Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae. Molecular Microbiology, 35(6), 1431-1442.
DOI Scopus197 WoS180 Europe PMC166
2000 Paton, A., Morona, R., & Paton, J. (2000). A new biological agent for treatment of Shiga toxigenic Escherichia coli infections and dysentery in humans. Nature Medicine, 6(3), 265-270.
DOI Scopus192 WoS167 Europe PMC128
1999 Daniels, C., & Morona, R. (1999). Analysis of Shigella flexneri Wzz (Rol) function by mutagenesis and cross-linking: Wzz is able to oligomerise.. Molecular Microbiology, 34(1), 181-194.
DOI Scopus91 WoS89 Europe PMC82
1999 Chua, J., Manning, P., & Morona, R. (1999). The Shigella flexneri bacteriophage Sf6 tailspike protein (TSP)/endorhamnosidase is related to the bacteriophage P22 TSP and has a motif common to exo- and endoglycanases, and C-5 epimerases. Microbiology - UK, 145(7), 1649-1659.
DOI Scopus40 WoS40 Europe PMC34
1999 Baker, S., Gunn, J., & Morona, R. (1999). The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance, and lipopolysaccharide.. Microbiology - UK, 145(2), 367-378.
DOI Scopus69 WoS67 Europe PMC62
1999 Morona, J., Miller, D., Coffey, T., Vindurampulle, C., Spratt, B., Morona, R., & Paton, J. (1999). Molecular and genetic characterization of the capsule biosynthesis locus of Streptococcus pneumoniae type 23F. Microbiology - UK, 145(4), 781-789.
DOI Scopus29 WoS23 Europe PMC21
1999 Morona, J., Morona, R., & Paton, J. (1999). Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types beloning to serogroup 19. Journal of Bacteriology, 181(17), 5355-5364.
DOI Scopus57 WoS53 Europe PMC48
1999 Morona, J., Morona, R., & Paton, J. (1999). Analysis of the 5' portion of the type 19A capsule locus identifies two classes of cpsC, cpsD, and cpsE genes in Streptococcus pneumoniae. Journal of Bacteriology, 181(11), 3599-3605.
DOI Scopus42 WoS36 Europe PMC32
1998 Coffey, T., Enright, M., Daniels, M., Morona, J., Morona, R., Hryniewicz, W., . . . Spratt, B. (1998). Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae.. Molecular Microbiology, 27(1), 73-83.
DOI Scopus283 WoS257 Europe PMC212
1998 Daniels, C., Vindurampulle, C., & Morona, R. (1998). Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy).. Molecular Microbiology, 28(6), 1211-1222.
DOI Scopus168 WoS169 Europe PMC141
1997 Van Den Bosch, L., Manning, P., & Morona, R. (1997). Regulation of O-antigen chain length is required for Shigella flexneri virulence. Molecular Microbiology, 23(4), 765-775.
DOI Scopus98 WoS96 Europe PMC93
1997 Mavris, M., Manning, P., & Morona, R. (1997). Mechanism of bacteriophage SfII-mediated sertype conversion in Shigella flexneri. Molecular Microbiology, 26(5), 939-950.
DOI Scopus99 WoS91 Europe PMC81
1997 Morona, J., Morona, R., & Paton, J. (1997). Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biuosynthetic pathway. Molecular Microbiology, 23(4), 751-763.
DOI Scopus103 WoS100 Europe PMC81
1997 Ogunniyi, A., Kotlarski, I., Morona, R., & Manning, P. (1997). Role of the SefA subunit protein of SEF14 fimbriae in the pathogenesis of Salmonella enterica serovar Enteritidis. Infection and Immunity, 65(February), 708-717.
1997 Baker, S., Daniels, C., & Morona, R. (1997). PHoP/Q regulated genes in Salmonella typhi: identification of melittin sensitive mutants. Microbial Pathogenesis, 22(3), 165-179.
DOI Scopus34 WoS32 Europe PMC30
1997 Paton, J., Morona, J., & Morona, R. (1997). Characterization of the Capsular Polysaccharide Biosynthesis Locus of Streptococcus pneumoniae Type 19F. Microbial Drug Resistance-Mechanisms Epidemiology and Disease, 3(1), 89-99.
DOI Scopus4 WoS5 Europe PMC4
1997 Morona, J., Morona, R., & Paton, J. (1997). Molecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B. Journal of Bacteriology, 179(15), 4953-4958.
DOI Scopus38 WoS35 Europe PMC32
1997 Ogunniyi, A. D., Kotlarski, I., Morona, R., & Manning, P. A. (1997). Role of SefA subunit protein of SEF14 fimbriae in the pathogenesis of Salmonella enterica serovar enteritidis. Infection and Immunity, 65(2), 708-717.
DOI Scopus43 WoS37 Europe PMC27
1995 Guildolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1995). Nucleotide sequence analysis of genes essential for production of Streptococcus pneumoniae type 19F capsular polysaccharide.. Developments in Biological Standardization, 85, 267-271.
Scopus3 WoS3 Europe PMC3
1995 Stroeher, U. H., Karageorgos, L. E., Morona, R., & Manning, P. A. (1995). In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon. Gene, 155(1), 67-72.
DOI Scopus16 WoS15 Europe PMC12
1995 Morona, R., Van Den Bosch, L., & Manning, P. (1995). Molecular, genetic and topological characterization of O-antigen chain length regulation in Shigella flexneri. Journal of Bacteriology, 177(4), 1059-1068.
DOI Scopus164 WoS118 Europe PMC144
1995 Stroeher, U., Karageorgos, L., Morona, R., & Manning, P. (1995). In Vibrio cholerae O1 rfaD is linked closely to rfb operon. Gene, 155(1), 67-72.
1995 Stroeher, U., Karageorgos, L., Brown, M., Morona, R., & Manning, P. (1995). A putative pathway for perosamine biosynthesis is the first function encoded within the rfb region of Vibrio cholerae O1. Gene, 166(1), 33-42.
DOI Scopus43 WoS44 Europe PMC38
1995 Morona, R., Karageorgos, L., Manning, P., & Stroeher, U. (1995). A putative pathway for biosynthesis of the O-antigen component, 3-deoxy-L-glycero-tetronic acid, based in the nucleotide sequence of the Vibrio cholerae O1 rfb region. Gene, 166(1), 19-31.
DOI Scopus12 WoS11 Europe PMC7
1995 Macpherson, D., Manning, P., & Morona, R. (1995). Genetic analysis of rfbX in Shigella flexneri. Gene, 155(1), 9-17.
DOI Scopus31 WoS33 Europe PMC26
1995 Manning, P., Karageorgos, L., & Morona, R. (1995). Putative O-antigen transport genes withing the rfbregion of Vibrio cholerae O1 are homologous to those for capsule transport. Gene, 158(1), 1-7.
DOI Scopus30 WoS28 Europe PMC26
1995 Jorgensen, L., O'Neill, B., Thomas, C., Morona, R., & Middelberg, A. (1995). Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press. Biotechnology Techniques, 9(7), 477-480.
DOI Scopus7 WoS6
1995 Morona, R., Macpherson, D., Van Den Bosch, L., Carlin, N., & Manning, P. (1995). Lipopolysaccharide with an altered O-antigen produced in Escherichia coli K-12 harbouring mutated, cloned Shigella flexneri rfb genes. Molecular Microbiology, 18(2), 209-223.
DOI Scopus31 WoS32 Europe PMC27
1995 Manning, P., Morona, R., & Rohde, M. (1995). Shigella: identifying the deadly diarroea. Today's Life Science, 7(3), 54-61.
1995 Stroeher, U., Dredge, B., Morona, R., Brown, M., Karageorgos, L., Albert, M., & Manning, P. (1995). Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139. Proceedings of the National Academy of Sciences of the United States of America, 92(22), 10374-10378.
DOI Scopus89 WoS81 Europe PMC65
1994 Morona, J. K., Guidolin, A., Morona, R., Hansman, D., & Paton, J. C. (1994). Isolation, characterization, and nucleotide sequence of IS1202, an insertion sequence of Streptococcus pneumoniae. Journal of Bacteriology, 176(14), 4437-4443.
DOI Scopus34 WoS39 Europe PMC24
1994 Macpherson, D. F., Manning, P. A., & Morona, R. (1994). Characterization of the dTDP‐rhamnose biosynthetic genes encoded in the rfb iocus of Shigella flexneri. Molecular Microbiology, 11(2), 281-292.
DOI Scopus75 WoS85 Europe PMC66
1994 Morona, R., Morona, J. K., Considine, A., Hackett, J. A., van den Bosch, L., Beyer, L., & Attridge, S. R. (1994). Construction of K88- and K99-expressing clones of Salmonella typhimurium G30: immunogenicity following oral administration to pigs. Vaccine, 12(6), 513-517.
DOI Scopus22 WoS15 Europe PMC15
1994 Morona, R., Mavris, M., Fallarino, A., & Manning, P. A. (1994). Characterization of the rfc region of Shigella flexneri. Journal of Bacteriology, 176(3), 733-747.
DOI Scopus132 WoS82 Europe PMC114
1994 Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F. Infection and Immunity, 62(12), 5384-5396.
DOI Scopus121 WoS119 Europe PMC96
1993 Francis, M. S., Parker, A. F., Morona, R., & Thomas, C. J. (1993). Bacteriophage lambda as a delivery vector for Tn10-derived transposons in Xenorhabdus bovienii. Applied and Environmental Microbiology, 59(9), 3050-3055.
DOI Scopus8 WoS10 Europe PMC3
1993 Paton, J. C., Morona, J. K., Harrer, S., Hansman, D., & Morona, R. (1993). Immunization of mice with Salmonella typhimurium C5 aroA expressing a genetically toxoided derivative of the pneumococcal toxin pneumolysin. Microbial Pathogenesis, 14(2), 95-102.
DOI Scopus9 WoS9 Europe PMC4
1992 Stroeher, U. H., Karageorgos, L. E., Morona, R., & Manning, P. A. (1992). Serotype conversion in Vibrio cholerae O1. Proceedings of the National Academy of Sciences of the United States of America, 89(7), 2566-2570.
DOI Scopus177 WoS166 Europe PMC124
1991 Morona, R., Yeadon, J., Considine, A., Morona, J. K., & Manning, P. A. (1991). Construction of plasmid vectors with a non-antibiotic selection system based on the Escherichia coli thyA+ gene: application to cholera vaccine development. Gene, 107(1), 139-144.
DOI Scopus39 WoS34 Europe PMC26
1991 Macpherson, D. F., Morona, R., Beger, D. W., Cheah, K., & Manning, P. A. (1991). Genetic analysis of the rfb region of Shigella flexneri encoding the Y serotype O‐antigen specificity. Molecular Microbiology, 5(6), 1491-1499.
DOI Scopus37 WoS43 Europe PMC35
1991 Attridge, S. R., Dearlove, C., Beyer, L., Van den Bosch, L., Howles, A., Hackett, J., . . . Rowley, D. (1991). Characterization and immunogenicity of EX880, a Salmonella typhi Ty21a-based clone which produces Vibrio cholerae O antigen. Infection and Immunity, 59(7), 2279-2284.
DOI Scopus16 WoS14 Europe PMC8
1991 Morona, R., Brown, M. H., Yeadon, J., Heuzenroeder, M. W., & Manning, P. A. (1991). Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12. FEMS Microbiology Letters, 82(3), 279-285.
DOI Scopus33 WoS36 Europe PMC17
1990 Tacket, C. O., Forrest, B., Morona, R., Attridge, S. R., LaBrooy, J., Tall, B. D., . . . Levine, M. M. (1990). Safety, immunogenicity, and efficacy against cholera challenge in humans of a typhoid-cholera hybrid vaccine derived from Salmonella typhi Ty21a. Infection and Immunity, 58(6), 1620-1627.
DOI Scopus75 WoS77 Europe PMC60
1990 Attridge, S. R., Daniels, D., Morona, J. K., & Morona, R. (1990). Surface co-expression of Vibrio cholerae and Salmonella typhi O-antigens on Ty21a clone EX210. Microbial Pathogenesis, 8(3), 177-188.
DOI Scopus18 WoS22 Europe PMC18
1990 Morona, R., Matthews, M. S., Morona, J. K., & BrownBrownBrown, M. H. (1990). Regions of the cloned Vibrio cholerae rfb genes needed to determine the Ogawa form of the O-antigen. MGG Molecular General Genetics, 224(3), 405-412.
DOI Scopus5 WoS3 Europe PMC3
1989 TACKET, C. O., FORREST, B., LOSONSKY, G., MORONA, R., ATTRIDGE, S. R., LABROOY, J., . . . LEVINE, M. M. (1989). SAFETY, IMMUNOGENICITY, AND EFFICACY OF A BIVALENT CHOLERA-TYPHOID HYBRID VACCINE. CLINICAL RESEARCH, 37(2), A442.
1989 Forrest, B. D., Labrooy, J. T., Attridoe, S. R., Boehm, G., Beyer, L., Morona, R., . . . Rowley, D. (1989). Lmmunogenicity of a candidate live oral typhoid/cholera hybrid vaccine in humans. Journal of Infectious Diseases, 159(1), 145-146.
DOI Scopus37 WoS33 Europe PMC24
1988 HONE, D. M., ATTRIDGE, S. R., FORREST, B., MORONA, R., DANIELS, D., LABROOY, J. T., . . . HACKETT, J. (1988). A GALE-VIA (VI-ANTIGEN-NEGATIVE) MUTANT OF SALMONELLA-TYPHI TY2 RETAINS VIRULENCE IN HUMANS. INFECTION AND IMMUNITY, 56(5), 1326-1333.
DOI WoS155 Europe PMC123
1988 Attridge, S., Hackett, J., Morona, R., & Whyte, P. (1988). Towards a live oral vaccine against enterotoxigenic Escherichia coli of swine. Vaccine, 6(5), 387-389.
DOI Scopus31 WoS26 Europe PMC17
1987 Hone, D., Morona, R., Attridge, S., & Hackett, J. (1987). Contruction of defined galE mutants of Salmonella for use as vaccines. Journal of Infectious Diseases, 156(1), 167-174.
DOI Scopus65 WoS68 Europe PMC54
1987 Ward, H., Morelli, G., Kamke, M., Morona, R., Yeadon, J., Hackett, J., & Manning, P. (1987). A physical map of the chromosomal region determining O-antigen biosynthesis in Vibrio cholerae 01. Gene, 55(2-3), 197-204.
DOI Scopus24 WoS26 Europe PMC21
1986 Alm, R. A., Braun, G., Morona, R., & Manning, P. A. (1986). Detection of an OmpA-like protein in Vibrio cholerae. FEMS Microbiology Letters, 37(1), 99-104.
DOI Scopus11 WoS13
1986 Morona, R., & Henning, U. (1986). New locus (ttr) in Escherichia coli K-12 affecting sensitivity to bacteriophage T2 and growth on oleate as the sole carbon source. Journal of Bacteriology, 168(2), 534-540.
DOI Scopus20 WoS21 Europe PMC17
1985 Morona, R., Kramer, C., & Henning, U. (1985). Bacteriophage receptor area of outer membrane protein OmpA of Escherichia coli K-12. Journal of Bacteriology, 164(2), 539-543.
DOI Scopus101 WoS106 Europe PMC93
1985 Morona, R., Krämer, C., & Henning, U. (1985). The nature of ompA mutants of Escherichia coli K12 exhibiting temperature-sensitive bacteriophage resistance. MGG Molecular General Genetics, 201(2), 357-359.
DOI
1985 Morona, R., Tommassen, J., & Henning, U. (1985). Demonstration of a bacteriophage receptor site on the Escherichia coli K12 outer-membrane protein OmpC by the use of a protease. European Journal of Biochemistry, 150(1), 161-169.
DOI Scopus40 WoS42 Europe PMC35
1984 Morona, R., & Henning, U. (1984). Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors. Journal of Bacteriology, 159(2), 579-582.
DOI Scopus41 WoS43 Europe PMC35
1984 Morona, R., Klose, M., & Henning, U. (1984). Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: Analysis of mutant genes expressing altered protein. Journal of Bacteriology, 159(2), 570-578.
DOI Scopus149 WoS172 Europe PMC148
1984 Morona, R., & Reeves, P. (1984). Detection of several diisopropylfluorophosphate-binding proteins in the outer membrane of Escherichia coli K-12. FEMS Microbiology Letters, 23(2-3), 179-182.
DOI Scopus3 WoS3
1983 Morona, R., Manning, P. A., & Reeves, P. (1983). Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. Journal of Bacteriology, 153(2), 693-699.
DOI Scopus49 WoS61 Europe PMC56
1982 Morona, R., & Reeves, P. (1982). A new locus, stc, which affects the phenotype of tolC mutants of Escherichia coli K-12. MGG Molecular General Genetics, 187(2), 335-341.
DOI Scopus5 WoS10
1982 Morona, R., & Reeves, P. (1982). The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins. Journal of Bacteriology, 150(3), 1016-1023.
DOI Scopus81 WoS114 Europe PMC96
1981 Morona, R., & Reeves, P. (1981). Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn 10. MGG Molecular General Genetics, 184(3), 430-433.
DOI Scopus16 WoS23 Europe PMC20
1980 MORONA, R., & REEVES, P. (1980). MOLECULAR-CLONING OF THE TO1C LOCUS WITH THE AID OF THE TN10 TRANSPOSON. PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13, 91.

Year Citation
2015 Standish, A., & Morona, R. (2015). Capsule structure, synthesis, and regulation. In J. Brown, S. Hammerschmidt, & C. Orihuela (Eds.), Streptococcus Pneumoniae: Molecular Mechanisms of Host-Pathogen Interactions (pp. 169-179). United Kingdom: Elsevier.
DOI Scopus4
2014 Manning, P. A., Stroeher, U. H., & Morona, R. (2014). Molecular Basis for O-Antigen Biosynthesis in Vibrio cholerae O1: Ogawa-Inaba Switching. In Vibrio Cholerae and Cholera Molecular to Global Perspectives (pp. 77-94). ASM Press.
DOI Scopus100
2011 Paton, A. W., Morona, R., & Paton, J. C. (2011). Designer Probiotics and Enteric Cytoprotection. In Probiotic Bacteria and Enteric Infections (pp. 429-443). Springer Netherlands.
DOI
2011 Paton, A., Morona, R., & Paton, J. (2011). Designer Probiotics and Enteric Cytoprotection. In J. Malago, J. Koninkx, & R. Marinsek Logar (Eds.), Probiotic Bacteria and Enteric Infections: Cytoprotection by Probiotic Bacteria (1 ed., pp. 429-444). Netherlands: Springer.
DOI

Year Citation
2008 Morona, J. K., Morona, R., & Paton, J. C. (2008). Comparison of Streptococcus pneumoniae Polysaccharide Polymerase Proteins Cps19aI and Cps19fI. Poster session presented at the meeting of FASEB Summer Research Conferences: Microbial Polysaccharides of Medical, Agricultural and Industrial Importance. Carefree, Arizona, USA.
2007 Morona, J. K., Morona, R., & Paton, J. C. (2007). Comparison of Streptococcus pneumoniae Polysaccharide Polymerase Proteins Cps19aI and Cps19fI. Poster session presented at the meeting of Ninth Australian Conference on Molecular Biology of Bacterial Pathogens. Lorne, Victoria, Australia.
2006 Morona, J. K., Morona, R., & Paton, J. C. (2006). Mutation of the Conserved Capsular Polysaccharide Biosynthesis Gene cpsB of Streptococcus pneumoniae D39 results in two distinct CPS phenotypes.. Poster session presented at the meeting of The 5th International Symposium on Pneumococci and Pneumococcal Diseases. Alice Springs, Northern Territory, Australia.
2000 Morona, J. K., Morona, R., Miller, D. C., & Paton, J.C.. (2000). Mutation analysis of the carboxy-terminal [YGX]4 repeat domain of CpsD, a Streptococcus pneumoniae capsule biosynthesis protein. Poster session presented at the meeting of FASEB Summer Research Conferences: Microbial Polysaccharides of Medical, Agricultural and Industrial Importance. Copper Mountain, Colorado, USA.
1999 Morona, J. K., Morona, R., & Paton, J. C. (1999). Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types belonging to serogroup 19. Poster session presented at the meeting of Fifth Australian Conference on Molecular Analysis of Bacterial Pathogens. Victor Harbor, SA., Australia.
1998 Paton, J. C., Morona, J. K., & Morona, R. (1998). Characterisation of the capsule biosynthesis loci of pneumococci belonging to serogroup 19. Poster session presented at the meeting of First International symposium on Pneumococci and pneumococcal Diseases. Elsinore, Denmark.
1997 Morona, J. K., Morona, R., & Paton, J. C. (1997). Molecular and genetic characterisation of the capsule biosynthesis locus of Streptococcus pneumoniae type 19B. Poster session presented at the meeting of Fourth Australian Conference on Molecular Analysis of Bacterial Pathogens. Jamberoo, NSW. Australia.
1997 Paton, J. C., Morona, J. K., & Morona, R. (1997). Molecular analysis of capsular polysaccharide biosynthesis (cps) loci of Streptococcus pneumoniae.. Poster session presented at the meeting of Microbiology Australia. Adelaide, Australia.
1994 Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Analysis of genes essential for the production of Streptococcus pneumoniae type 19F capsular polysaccharide. Poster session presented at the meeting of Am. Soc. Microbiol. Fourth Int. Conf. Streptoccocal Genet. Sante Fe, New Mexico, USA.
1994 Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Nucleotide sequence of genes essential for production of Streptococcus pneumoniae type 19F capsular polysaccharide. Poster session presented at the meeting of Australian Microbiologist. Melbourne, Australia.

Year Citation
2024 Qin, J., Hong, Y., Morona, R., & Totsika, M. (2024). Tolerance Mechanisms in Polysaccharide Biosynthesis: Implications for Undecaprenol Phosphate Recycling in<i>Escherichia coli</i>and<i>Shigella flexneri</i>.
DOI Europe PMC1





 

B.Sc.Honours Co-ordinator

Course Co-ordinator for Infection and Immunity A III

Date Role Research Topic Program Degree Type Student Load Student Name
2021 - 2024 Principal Supervisor The Biogenesis and Homeostasis of the Shigella flexneri Cell Envelope Doctor of Philosophy Doctorate Full Time Miss Alice Ascari
2018 - 2022 Principal Supervisor Enterobacterial Common Antigen Biosynthesis in Shigella flexneri Doctor of Philosophy Doctorate Full Time Mr Nicholas Tadeusz Maczuga
2017 - 2021 Principal Supervisor Investigation of the Wzy O Antigen Polymerase and the Wzz Co-Polymerase Interface Doctor of Philosophy Doctorate Full Time Mr Vincenzo Leo
2016 - 2020 Principal Supervisor Investigation of the IcsA-mediated Shigella flexneri hyper-adherence Doctor of Philosophy Doctorate Full Time Mr Jilong Qin
2014 - 2018 Principal Supervisor Investigation of a Low Molecular Weight Protein Tyrosine Phosphatase in Streptococcus Pneumoniae Doctor of Philosophy Doctorate Full Time Miss Zuleeza Ahmad
2013 - 2015 Principal Supervisor Polarity and Secretion of Shigella flexneri IcsA: A Classical Autotransporter Doctor of Philosophy Doctorate Full Time Mr Matthew Thomas Doyle
2011 - 2015 Principal Supervisor Characterisation of the Shigella flexneri O Antigen Polymerase Wzy Doctor of Philosophy Doctorate Full Time Ms Pratiti Nath
2011 - 2015 Co-Supervisor STAPHYLOCOCCUS AUREUS: STRESS RESPONSE AND ITS ROLES IN PATHOGENESIS Doctor of Philosophy Doctorate Full Time Miss Minh Giao Long Bui
2009 - 2014 Co-Supervisor Coupling Stress Responses and Growth Pathways in Haemophilus influenzae Doctor of Philosophy Doctorate Full Time Mr Changde Donald Jiang
2009 - 2014 Principal Supervisor Identification of host cell proteins involved in Shigella flexneri pathogenesis Doctor of Philosophy Doctorate Full Time Miss Mabel Lum
2009 - 2012 Co-Supervisor Cell Biology of the Interaction between Listeria monocytogenes and Colpoda spp. Doctor of Philosophy Doctorate Full Time Mr Rethish Raghu Nadhanan
2008 - 2012 Co-Supervisor The Role of CpsC in the Regulation of Streptococcus pneumoniae Capsular Polysaccharide Biosynthesis Doctor of Philosophy Doctorate Full Time Mr James Byrne
2007 - 2009 Co-Supervisor Role of pCT0018 for Copper Homeostasis in Listeria monocytogenes Strain DRDC8 Master of Science Master Full Time Miss Mei Mei Hii
2007 - 2013 Principal Supervisor Analysis of Shigella flexneri Cell Surface Virulence Factors Doctor of Philosophy Doctorate Full Time Dr Min Teh
2007 - 2012 Co-Supervisor The Role of YeaZ in the VBNC State of Vibrio parahaemolyticus NCTC 10884 Doctor of Philosophy Doctorate Full Time Mr Shih-Hsun Chen
2006 - 2010 Principal Supervisor Biogenesis of Shigella flexneri IcsA Protein Doctor of Philosophy Doctorate Full Time Mr Marcin Grabowicz
2005 - 2011 Principal Supervisor Characterisation of Shigella flexneri polysaccharide co-polymerase (PCP) protein Wzz Doctor of Philosophy Doctorate Full Time Ms Maddy Papadopoulos
2004 - 2008 Principal Supervisor Molecular Characterisation of Shigella flexneri Outer Membrane Protease IcsP Doctor of Philosophy Doctorate Part Time Dr Elizabeth Tran
2003 - 2007 Principal Supervisor Molecular Characterisation of Shigella flexneri IcsA and the Role Of Lipopolysaccharide O-Antigen in Actin-based Motility Doctor of Philosophy Doctorate Full Time Dr Kerrie May
2000 - 2005 Co-Supervisor The role of wzz genes in Salmonella typhimurium virulence Doctor of Philosophy Doctorate Full Time Mr Gerald Murray

Date Role Membership Country
2017 - ongoing Member Fellow of the Australian Society for Microbiology (FASM) Australia
1990 - ongoing Member American Society for Microbiology United States

Connect With Me

External Profiles

Other Links