Renato Morona

School of Biological Sciences

Faculty of Sciences, Engineering and Technology

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

My laboratory studies the human specific bacterial pathogen Shigella flexneri which causes bacillary dysentery.
Current research areas are :
1) Role of membrane proteins in biosynthesis of LPS O antigen and Enterobacterial Common Antigen
2) Function of LPS O antigen in pathogenesis
3) Function of IcsA protein in pathogenesis

My Research
Career
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Grants and Funding
Teaching
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Invasive Bacterial Pathogens Laboratory

My laboratory conducts research into a number of enteric and invasive bacterial pathogens with a focus on the role of bacterial cell surface molecules in bacterial pathogenesis.

My research interests are:

Shigella flexneri

Actin-based motility

Lipopolysaccharides and O Antigens

Regulation of Capsule Polysaccharides

Outer Membrane Proteins

Bacteriophage

Receptor Mimic Technology

Anti-microbial drug discovery

PhD and Honours Projects

A number of challenging projects are available to interested PhD and Honours students. These projects will be in the general area of Shigella flexneri virulence factors, with an emphasis on the structure and function of IcsA, on lipopolysaccharide (LPS) O antigen biosynthesis proteins, and bacterial cell biology. Project information is available in the Honours and PhD page of the School website.

Other Pathogens we have Studied Include:

Salmonella enterica Typhi

Salmonella enterica Typhimurium

Vibrio cholerae O1

Shigella flexneri

Shigella flexneri infected HeLa cells

Shigella flexneri are Gram-negative bacteria that cause bacillary dysentery (shigellosis) in humans, and result in over 100 million episodes of diarrhea and 1000's of deaths each year.

Shigellosis arises due to poor sanitation and hygiene, and is transmitted by the oral-faecal route. Antibiotic therapy is frequently ineffective due to multi-antibiotic resistance. Shigellae are facultative intracellular pathogens able to invade, replicate within, and kill intestinal cells, and can also spread from cell to cell without going through an extracellular stage.

The infection results in an acute inflammatory response in the colon that causes the majority of the disease symptoms. S. flexneri bacteria are able to invade intestinal cells and replicate within them. After escaping into the cytosol, they replicate and exhibit intracellular motility in which the polarly localized cell surface protein IcsA recruits host proteins to generate actin-based motility (ABM). ABM allows the bacteria to spread from cell to cell thereby creating a focus of infection.

Relevant Publications

Lum, M., and Morona R. (2014) Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection. Int J Med Microbiol. 2014 Apr 3. pii: S1438-4221(14)00027-7. doi: 10.1016/j.ijmm.2014.03.006. [Epub ahead of print]

Lum, M., and Morona R. (2014) Myosin IIA is essential for Shigella flexneri cell-to-cell spread. Pathog Dis. doi: 10.1111/2049-632X.12202.

Lum, M., Attridge, S.R., and Morona R. (2013) Impact of dynasore an inhibitor of dynamin II on Shigella flexneri Infection. PLoS One. 8(12):e84975. doi: 10.1371/journal.pone.0084975. eCollection 2013.

Actin Based Motility

Actin based motility (ABM) is used by a number of microbial pathogens to move inside and between infected host (animal and human) cells. In Shigella flexneri, the outer membrane IcsA protein is reponsible for nucleating actin polymerisation by subverting host cell actin and actin regulatory proteins (e.g. N-WASP, Arp2/3 complex, Profilin, Cofilin, CapZ).

ABM can be visualized by staining using FITC-phalloidin to detect the F-actin comet tails that are formed. Additionally, time-lapse video microscopy can be used to visualise and measure the rate of motility.

In addition to IcsA, bacterial factors such as lipopolysaccharides also impact on IcsA function and intercellular spreading.

Relevant Publications

Morona, R., and Van Den Bosch, L. (2003) "Lipopolysaccharide O antigen chains mask IcsA(VirG) in Shigella flexneri" FEMS Microbiol. Lett. 221: 173-180.

Morona, R., and Van Den Bosch, L. (2003) "Multi-copy icsA is able to suppress the virulence defect caused by the wzzSF mutation in Shigella flexneri." FEMS Microbiol. Lett. 221: 213-219.

Van Den Bosch, L. and Morona, R. (2003) "The actin-based motility defect of Shigella flexneri rmlD rough LPS mutant is not due to loss of IcsA polarity." Microb. Pathogen. 35: 11-18.

Van Den Bosch, L., Manning, P.A., and Morona, R. (1997) “Regulation of O-antigen chain length is required for Shigella flexneri virulence”. Mol. Microbiol. 23: 765-775

Lipopolysaccharides

Lipopolysaccharide (LPS) molecules are located in the outer leaflet of the outer membrane of bacteria such as Shigella flexneri and Salmonella enterica Typhimurium. LPS molecules are amphipathic and have three regions: lipid A, core sugars, and O antigen polysaccharides. The O antigen polysaccharides of S. flexneri and S. Typhimurium are composed of repeats of a tetrasaccharide unit, and range from 1 to >100 repeat units in length. The length of O antigen polysaccharides is regulated by the activity of Wzz proteins, with different Wzz proteins able to confer different modal length distributions. O antigen polysaccharide chain length impacts on virulence as it affects resistance to Complement, and actin based motility.

Relevant Publications

Kalynych, S., Morona, R., and Miroslaw, C. (2014) Progress in understanding the assembly process of bacterial O-Antigen. FEMS Microbiol Rev. 2014 Mar 11. doi: 10.1111/1574-6976.12070. [Epub ahead of print]

Tran, E.N.H. and Morona R. (2013) Residues located inside the Escherichia coli FepE protein oligomer are essential for lipopolysaccharide O-antigen modal chain length regulation. Microbiology (SGM). 159: 701-714.

Papadopoulos, M and Morona, R. (2010) Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. J. Bacteriol. 192: 3385-3393.

Morona, R., Purins, L., Tocilj, A., Matte, A., and Cygler M. (2009) Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins. Trends Biochem Sci. 34:78-84.

Purins, L., Van Den Bosch, L., and Richardson, V., and Morona, R. (2008) Coiled-coil regions play a role in the function of the Shigella flexneri O-antigen chain length regulator WzzpHS2. Microbiology (SGM) 154:1104-1116.

Tocilj, A., Munger, C., Proteau, A., Morona, R., Purins, L., Ajamina, E., Wagner, J., Papadopoulos, M., Van Den Bosch, L., Rubinstein, J.L., Féthière, J., Matte, A., and Cygler, M. (2008) Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Nature Struct. Mol. Biol. 15:130-138.

Murray, G.L., Attridge, S.R., and Morona, R. (2006) Altering the length of Salmonella typhimurium lipopolysaccharide O antigen has an impact on interaction with macrophages and complement". J. Bacteriol. 188: 2735-2739.

Murray, G.L., Attridge, S.R., and Morona, R. (2005) "Inducible serum resistance in Salmonella typhimurium is dependent on wzz(fepE)-regulated very long O antigen chains". Microbes Infect. 7: 1296-1304.

Morona, R., Daniels, C., and Van Den Bosch, L. (2003) “Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimised for virulence.” Microbiology, 49: 925-939.

Murray, G.L., Attridge, S.R., and Morona, R. (2003) “Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz.” Mol. Microbiol. 47: 1395-1406.

Morona, R., and Van Den Bosch, L. (2003) "Lipopolysaccharide O antigen chains mask IcsA(VirG) in Shigella flexneri" FEMS Microbiol. Lett. 221: 173-180.

Morona, R., Van Den Bosch, L., and Daniels, C. (2000) “Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions.” Microbiol. 146: 1-4.

Table for this paper.

Baker, S.J., Gunn, J.S. and Morona, R. (1999) “The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance, and lipopolysaccharide.” Microbiol. 145: 367-378.

Daniels, C., and Morona, R. (1999) Analysis of Shigella flexneri Wzz (Rol) function by mutagenesis and cross-linking: Wzz is able to oligomerise. Mol. Microbiol. 34: 181-194.

Daniels, C., Vindurampulle, C., and Morona, R. (1998) Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy). Mol. Microbiol. 28: 1211-1222.

Van Den Bosch, L., Manning, P.A., and Morona, R. (1997) “Regulation of O-antigen chain length is required for Shigella flexneri virulence”. Mol. Microbiol. 23: 765-775.

Outer Membrane Proteins

The outer membrane (OM) of Gram-negative bacteria is in direct contact with the external environment, and contains proteins, lipoproteins, phospholipids, and lipopolysaccharides (LPSs). OM proteins (OMPs) are often directly involved in the virulence of pathogenic bacteria. The Shigella flexneri OMP called IcsA protein is essential for bacterial motility inside infected human cells, while another S. flexneri OMP is IcsP which is a protease that cleaves IcsA.

IcsA and IcsP

The IcsA protein is a large 116 kDa outer membrane protein which is able to interact with actin regulatory proteins within the cytosol of host (human and animal) cells to trigger polymerisation of actin and hence actin-based motility. IcsA is a member of the autotransporter family of proteins, and has an 85 kDa amino terminal passenger (or alpha domain) and a 37 kDa carboxy terminal transporter (or beta domain) that is located in the outer membrane. The IcsA protein is it is targeted to and predominantly localised at the old cell pole in strains that have intact LPS. Polar localisation is relaxed in strains unable to produce either LPS O antigens or the IcsP protease.

Relevant Publications

Zumsteg, A.B., Goosmann, C., Brinkmann, V., Morona, R., and Arturo Zychlinsky, A. (2014) IcsA is an adhesin in Shigella flexneri that is regulated by the activity of the Type III secretion system. Cell Host and Microbe 15:435-445.

Tran, E.N, Doyle, M.T, and Morona R. (2013) LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles. PLoS One. 2013 Jul 25;8(7):e70508. doi: 10.1371/journal.pone.0070508.

Teh, M.Y. and Morona, R. (2013) Identification of Shigella flexneri IcsA residues affecting interaction with N-WASP, and evidence for IcsA-IcsA co-operative interaction. PLoS One. 2013; 8(2):e55152. doi: 10.1371/journal.pone.0055152. Epub 2013 Feb 6.

Teh, M.Y., Tran, E.N., and Morona, R. (2012) Absence of O-antigen suppresses Shigella flexneri IcsA autochaperone region mutations. Microbiology (SGM). 158: 2835-2850.

May, K.L., Grabowicz, M., Polyak, S.W. and Morona, R. (2012) Self-association of the Shigella flexneri IcsA autotransporter. Microbiology (SGM). 158:1874-1883.

Lum, M. and Morona, R. (2012) IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface. Microbial Cell Factories. 11:20. doi: 10.1186/1475-2859-11-20.

May, K.M. and Morona, R. (2008) Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. J Bacteriol. 190:4666-4676.

Education

Date Institution name Country Title

1982 University of Adelaide Australia PhD

1978 University of Adelaide Australia B.Sc. (Hons)

1977 University of Adelaide Australia B.Sc.
Research Interests

Animal Cell and Molecular Biology Biochemistry & Molecular Biology Cell Biology Infectious Diseases Innate Immunity Medical Infection Agents Medical Microbiology Microbial Genetics Microbiology Microscopy

Date	Institution name	Country	Title
1982	University of Adelaide	Australia	PhD
1978	University of Adelaide	Australia	B.Sc. (Hons)
1977	University of Adelaide	Australia	B.Sc.

Journals

Year	Citation
2023	Ascari, A., Waters, J. K., Morona, R., & Eijkelkamp, B. A. (2023). Shigella flexneri Adapts to Niche-Specific Stresses through Modifications in Cell Envelope Composition and Decoration. ACS Infectious Diseases, 9(8), 1610-1621. DOI Scopus3 Europe PMC2
2023	Qin, J., Hong, Y., Morona, R., & Totsika, M. (2023). O antigen biogenesis sensitises Escherichia coli K-12 to bile salts, providing a plausible explanation for its evolutionary loss. PLoS Genetics, 19(10), e1010996-1-e1010996-21. DOI Scopus4 Europe PMC2
2023	Ascari, A., Frölich, S., Zang, M., Tran, E. N. H., Wilson, D. W., Morona, R., & Eijkelkamp, B. A. (2023). Shigella flexneri remodeling and consumption of host lipids during infection. Journal of Bacteriology, 205(12), 1-18. DOI
2022	Ascari, A., Tran, E. N. H., Eijkelkamp, B. A., & Morona, R. (2022). Detection of a disulphide bond and conformational changes in Shigella flexneri Wzy, and the role of cysteine residues in polymerase activity. Biochimica et Biophysica Acta (BBA) - Biomembranes, 1864(5), 183871-1-183871-12. DOI Scopus6 WoS2 Europe PMC4
2022	Teh, M. Y., Tran, E. N. H., & Morona, R. (2022). Bacteriophage Sf6 host range mutant that infects Shigella flexneri serotype 2a₂ strains. FEMS Microbiology Letters, 369(1), 1-5. DOI Scopus3 WoS1 Europe PMC2
2022	Maczuga, N., Tran, E. N. H., Qin, J., & Morona, R. (2022). Interdependence of Shigella flexneri O Antigen and Enterobacterial Common Antigen Biosynthetic Pathways. Journal of Bacteriology, 204(4), 1-16. DOI Scopus10 WoS5 Europe PMC8
2022	Maczuga, N. T., Tran, E. N. H., & Morona, R. (2022). Topology of the Shigella flexneri Enterobacterial Common Antigen polymerase WzyE. Microbiology, 168(4), 001183-1-001183-11. DOI Scopus3 WoS2 Europe PMC3
2022	Qin, J., Hong, Y., Pullela, K., Morona, R., Henderson, I. R., & Totsika, M. (2022). A method for increasing electroporation competence of Gram-negative clinical isolates by polymyxin B nonapeptide. Scientific Reports, 12(1), 11629-1-11629-8. DOI Scopus7 WoS1 Europe PMC5
2022	Qin, J., Hong, Y., Morona, R., & Totsika, M. (2022). Cysteine dependent conformation heterogeneity of <i>Shigella flexneri</i> autotransporter IcsA and implications in its function. DOI
2022	Ascari, A., Tran, E. N. H., Eijkelkamp, B. A., & Morona, R. (2022). Identification of the Shigella flexneri Wzy Domain Modulating WzzpHS-2 Interaction and Detection of the Wzy/Wzz/Oag Complex. Journal of Bacteriology, 204(9), e0022422-1-e0022422-18. DOI Scopus6 WoS3 Europe PMC4
2022	Maczuga, N., Tran, E. N. H., & Morona, R. (2022). Subcellular localization of the enterobacterial common antigen GT-E-like glycosyltransferase, WecG. Molecular Microbiology, 118(4), 403-416. DOI Europe PMC1
2022	Qin, J., Hong, Y., Morona, R., & Totsika, M. (2022). Cysteine-Dependent Conformational Heterogeneity of Shigella flexneri Autotransporter IcsA and Implications of Its Function. Microbiology Spectrum, 10(6), 1-16. DOI Scopus3 WoS1 Europe PMC3
2021	Doore, S. M., Subramanian, S., Tefft, N. M., Morona, R., TerAvest, M. A., & Parent, K. N. (2021). Large metabolic rewiring from small genomic changes between strains of Shigella flexneri. Journal of Bacteriology, 203(11), 1-13. DOI Scopus5 WoS3 Europe PMC4
2021	Leo, V., Teh, M. Y., Tran, N., & Morona, R. (2021). Identification of a region in Shigella flexneri WzyB disrupting the interaction with WzzpHS2. Journal of Bacteriology, 203(22), e00413-21-1-e00413-21-15. DOI Scopus5 WoS4 Europe PMC3
2020	Leo, V., Tran, N., & Morona, R. (2020). Polysaccharide co-polymerase WzzB/WzzE chimeras reveal transmembrane 2 region of WzzB is important for interaction with WzyB. Journal of Bacteriology, 203(6), 1-14. DOI Scopus11 WoS5 Europe PMC9
2020	Tran, N. H., Day, C. J., Poole, J., Jennings, M. P., & Morona, R. (2020). Specific blood group antibodies inhibit Shigella flexneri interaction with human cells in the absence of spinoculation. Biochemical and Biophysical Research Communications, 521(1), 131-136. DOI Scopus5 WoS3 Europe PMC3
2020	Qin, J., Doyle, M. T., Tran, E. N. H., & Morona, R. (2020). The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function.. PloS one, 15(1), e0227425. DOI Scopus10 WoS7 Europe PMC8
2020	Tran, E. N. H., Day, C. J., McCartney, E., Poole, J., Tse, E., Jennings, M. P., & Morona, R. (2020). Shigella flexneri targets human colonic goblet cells by O antigen binding to Sialyl-Tn and Tn antigens via glycan-glycan interactions. ACS Infectious Diseases, 6(10), 2604-2615. DOI Scopus9 WoS4 Europe PMC7
2020	Teh, M. Y., Furevi, A., Widmalm, G., & Morona, R. (2020). Influence of Shigella flexneri 2a O antigen acetylation on its bacteriophage Sf6 receptor activity, and bacterial interaction with human cells. Journal of Bacteriology, 202(24), 1-19. DOI Scopus7 WoS6 Europe PMC6
2019	Helbig, K. J., Teh, M. Y., Crosse, K. M., Monson, E. A., Smith, M., Tran, E. N., . . . Beard, M. R. (2019). The interferon stimulated gene viperin, restricts Shigella. flexneri in vitro.. Sci Rep, 9(1), 15598. DOI Scopus17 WoS16 Europe PMC11
2018	Ahmad, Z., Harvey, R., Paton, J., Standish, A., & Morona, R. (2018). Role of Streptococcus pneumoniae OM001 operon in capsular polysaccharide production, virulence and survival in human saliva. PLoS ONE, 13(1), 1-15. DOI Scopus3 WoS3 Europe PMC3
2018	Ahmad, Z., Morona, R., & Standish, A. (2018). In vitro characterization and identification of potential substrates of a low molecular weight protein tyrosine phosphatase in Streptococcus pneumoniae. Microbiology, 164(4), 697-703. DOI Scopus3 WoS3 Europe PMC1
2016	Standish, A., Teh, M., Tran, E., Doyle, M., Baker, P., & Morona, R. (2016). Unprecedented abundance of protein tyrosine phosphorylation modulates Shigella flexneri virulence. Journal of Molecular Biology, 428(20), 4197-4208. DOI Scopus20 WoS19 Europe PMC14
2016	Papadopoulos, M., Tran, E., Murray, G., & Morona, R. (2016). Conserved transmembrane glycine residues in the shigella flexneri polysaccharide co-polymerase protein WzzB influence protein-protein interaction. Microbiology, 162(6), 921-929. DOI Scopus5 WoS4 Europe PMC4
2015	Doyle, M., Grabowicz, M., May, K., & Morona, R. (2015). Lipopolysaccharide surface structure does not influence IcsA polarity. FEMS Microbiology Letters, 362(8), fnv042-1-fnv042-7. DOI Scopus2 WoS2 Europe PMC1
2015	Doyle, M., Tran, E., & Morona, R. (2015). The passenger-associated transport repeat promotes virulence factor secretion efficiency and delineates a distinct autotransporter subtype. Molecular microbiology, 97(2), 315-329. DOI Scopus9 WoS6 Europe PMC5
2015	Tran, E., Attridge, S., Teh, M., & Morona, R. (2015). Shigella flexneri cell-to-cell spread, and growth and inflammation in mice, is limited by the outer membrane protease IcsP. FEMS Microbiology Letters, 362(12), fnv088-1-fnv088-8. DOI Scopus1 WoS1 Europe PMC1
2015	Paton, A., Chen, A., Wang, H., McAllister, L., Höggerl, F., Mayr, U., . . . Paton, J. (2015). Protection against Shiga-toxigenic Escherichia coli by non-genetically modified organism receptor mimic bacterial ghosts. Infection and Immunity, 83(9), 3526-3533. DOI Scopus8 WoS13 Europe PMC4
2015	Nath, P., & Morona, R. (2015). Detection of Wzy/Wzz interaction in Shigella flexneri. Microbiology, 161(9), 1797-1805. DOI Scopus27 WoS21 Europe PMC19
2015	Doyle, M., Grabowicz, M., & Morona, R. (2015). A small conserved motif supports polarity augmentation of Shigella flexneri IcsA. Microbiology, 161(11), 2087-2097. DOI Scopus6 WoS5 Europe PMC5
2015	Chang, C., Tran, E., Ericsson, D., Casey, L., Lonhienne, T., Benning, F., . . . Kobe, B. (2015). Structural and biochemical analysis of a single amino-acid mutant of WzzBSF that alters lipopolysaccharide O-antigen chain length in shigella flexneri. PLoS One, 10(9), e0138266-1-e0138266-21. DOI Scopus10 WoS8 Europe PMC5
2015	Day, C., Tran, E., Semchenko, E., Tram, G., Hartley-Tassell, L., Ng, P., . . . Jennings, M. (2015). Glycan: glycan interactions: high affinity biomolecular interactions that can mediate binding of pathogenic bacteria to host cells. Proceedings of the National Academy of Sciences of the United States of America, 112(52), E7266-E7275. DOI Scopus99 WoS83 Europe PMC52
2015	Whittall, J., Morona, R., & Standish, A. (2015). Topology of Streptococcus pneumoniae CpsC, a Polysaccharide Copolymerase and Bacterial Protein Tyrosine Kinase Adaptor Protein. Journal of Bacteriology, 197(1), 120-127. DOI Scopus6 WoS6 Europe PMC6
2015	Nath, P., Tran, E., & Morona, R. (2015). Mutational analysis of the Shigella flexneri O-antigen polymerase Wzy: Identification of Wzz-dependent Wzy mutants. Journal of Bacteriology, 197(1), 108-119. DOI Scopus14 WoS12 Europe PMC7
2015	Nath, P., & Morona, R. (2015). Mutational analysis of the major periplasmic loops of Shigella flexneri Wzy; identification of the residues affecting O antigen modal chain length control, and Wzz dependent polymerisation activity. Microbiology, 161(4), 774-785. DOI Scopus18 WoS15 Europe PMC8
2014	Standish, A., Whittall, J., & Morona, R. (2014). Tyrosine phosphorylation enhances activity of pneumococcal autolysin LytA. Microbiology, 160(12), 2745-2754. DOI Scopus15 WoS12 Europe PMC10
2014	Lum, M., & Morona, R. (2014). Myosin IIA is essential for Shigella flexneri cell-to-cell spread. Pathogens and Disease, 72(3), 174-187. DOI Scopus15 WoS14 Europe PMC13
2014	Lum, M., & Morona, R. (2014). Dynamin-related protein Drp1 and mitochondria are important for Shigella flexneri infection. International Journal of Medical Microbiology, 304(5-6), 530-541. DOI Scopus29 WoS21 Europe PMC16
2014	Zumsteg, A., Goosmann, C., Brinkmann, V., Morona, R., & Zychlinsky, A. (2014). IcsA is a Shigella flexneri adhesin regulated by the type III secretion system and required for pathogenesis. Cell Host and Microbe, 15(4), 435-445. DOI Scopus78 WoS36 Europe PMC56
2014	Kalynych, S., Morona, R., & Cygler, M. (2014). Progress in understanding the assembly process of bacterial O-antigen. FEMS Microbiology Reviews, 38(5), 1048-1065. DOI Scopus86 WoS79 Europe PMC61
2014	Tran, E. N. H., Doyle, M. T., & Morona, R. (2014). Correction: LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles (PLoS ONE (2013) 8, 7 (e70508) DOI: 10.1371/journal.pone.0070508). PLoS ONE, 9(10), e111656. DOI
2014	Standish, A., & Morona, R. (2014). The role of bacterial protein tyrosine phosphatases in the regulation of the biosynthesis of secreted polysaccharides. Antioxidants & Redox Signaling, 20(14), 2274-2289. DOI Scopus37 WoS30 Europe PMC26
2014	Tran, N., Papadopoulos, M., & Morona, R. (2014). Relationship between O-antigen chain length and resistance to colicin E2 in Shigella flexneri. Microbiology, 160(3), 589-601. DOI Scopus27 WoS23 Europe PMC20
2013	Lum, M., Attridge, S., & Morona, R. (2013). Impact of dynasore an inhibitor of dynamin II on Shigella flexneri infection. PLoS One, 8(12), e84975-1-e84975-17. DOI Scopus12 WoS9 Europe PMC7
2013	George, D., Stephenson, D., Tran, E., Morona, R., & Verma, N. (2013). Complete genome sequence of SfII, a serotype-converting bacteriophage of the highly prevalent Shigella flexneri serotype 2a. Genome Announcements, 1(5), e00626-13. DOI Scopus7 WoS6 Europe PMC6
2013	Morona, R. (2013). Encapsulating bacteria. Structure, 21(5), 692-693. DOI Scopus1 WoS1 Europe PMC1
2013	Tran, N., Doyle, M., & Morona, R. (2013). LPS unmasking of Shigella flexneri reveals preferential localisation of tagged outer membrane protease IcsP to septa and new poles. PLoS One, 8(7), 1-16. DOI Scopus15 WoS16 Europe PMC11
2013	Tran, N., & Morona, R. (2013). Residues located inside the Escherichia coli FepE protein oligomer are essential for lipopolysaccharide O-antigen modal chain length regulation. Microbiology, 159(4), 701-714. DOI Scopus16 WoS15 Europe PMC15
2013	Teh, M., & Morona, R. (2013). Identification of Shigella flexneri IcsA residues affecting interaction with N-WASP, and evidence for IcsA-IcsA co-operative interaction. PLoS One, 8(2), 1-17. DOI Scopus10 WoS11 Europe PMC8
2013	Standish, A., Salim, A., Capon, R., & Morona, R. (2013). Dual inhibition of DNA polymerase PolC and protein tyrosine phosphatase CpsB uncovers a novel antibiotic target. Biochemical and Biophysical Research Communications, 430(1), 167-172. DOI Scopus13 WoS11 Europe PMC12
2012	Teh, M., Tran, N., & Morona, R. (2012). Absence of O antigen suppresses Shigella flexneri IcsA autochaperone region mutations. Microbiology, 158(11), 2835-2850. DOI Scopus12 WoS13 Europe PMC10
2012	Watts, R., Tan, C., Ulett, G., Carey, A., Totsika, M., Idris, A., . . . Schembri, M. (2012). Escherichia coli 83972 expressing a P fimbriae oligosaccharide receptor mimic impairs adhesion of uropathogenic E. coli. Journal of Infectious Diseases, 206(8), 1242-1249. DOI Scopus25 WoS18 Europe PMC15
2012	Ericsson, D., Standish, A., Kobe, B., & Morona, R. (2012). Wzy-dependent bacterial capsules as potential drug targets. Current Drug Targets, 13(11), 1421-1431. DOI Scopus14 WoS12 Europe PMC9
2012	May, K., Grabowicz, M., Polyak, S., & Morona, R. (2012). Self-association of the Shigella flexneri IcsA autotransporter protein. Microbiology, 158(7), 1874-1883. DOI Scopus7 WoS8 Europe PMC7
2012	Paton, A., Morona, R., & Paton, J. (2012). Bioengineered microbes in disease therapy. Trends in Molecular Medicine, 18(7), 417-425. DOI Scopus40 WoS29 Europe PMC26
2012	Standish, A., Salim, A., Zhang, H., Capon, R., & Morona, R. (2012). Chemical inhibition of bacterial protein tyrosine phosphatase suppresses capsule production. PLoS One, 7(5), 1-8. DOI Scopus27 WoS24 Europe PMC19
2012	Soares da Costa, T., Tieu, W., Yap, M., Pendini, N., Polyak, S., Pedersen, D., . . . Abell, A. (2012). Selective inhibition of biotin protein ligase from Staphylococcus aureus. Journal of Biological Chemistry, 287(21), 17823-17832. DOI Scopus59 WoS45 Europe PMC31
2012	Zvarec, O., Polyak, S., Tieu, W., Kuan, K., Dai, H., Pedersen, D., . . . Abell, A. (2012). 5-Benzylidenerhodanine and 5-benzylidene-2-4-thiazolidinedione based antibacterials. Bioorganic & Medicinal Chemistry Letters, 22(8), 2720-2722. DOI Scopus42 WoS34 Europe PMC15
2012	Lum, M., & Morona, R. (2012). IcsA autotransporter passenger promotes increased fusion protein expression on the cell surface. Microbial Cell Factories, 11(1), 1-10. DOI Scopus15 WoS14 Europe PMC12
2011	Byrne, J., Morona, J., Paton, J., & Morona, R. (2011). Identification of Streptococcus pneumoniae Cps2C residues that affect capsular polysaccharide polymerization, cell wall ligation, and Cps2D phosphorylation. Journal of Bacteriology, 193(9), 2341-2346. DOI Scopus17 WoS14 Europe PMC12
2010	Paton, A., Morona, R., & Paton, J. (2010). Bioengineered bugs expressing oligosaccharide receptor mimics: Toxin-binding probiotics for treatment and prevention of enteric infections. Bioengineered Bugs, 1(3), 172-177. DOI Scopus27 Europe PMC14
2010	Papadopoulos, M., & Morona, R. (2010). Mutagenesis and chemical cross-linking suggest that Wzz dimer stability and oligomerization affect lipopolysaccharide O-antigen modal chain length control. Journal of Bacteriology, 192(13), 3385-3393. DOI Scopus28 WoS28 Europe PMC22
2010	Paton, A., Morona, R., & Paton, J. (2010). Receptor-mimic probiotics: Potential therapeutics for bacterial toxin-mediated enteric diseases. Expert Review of Gastroenterology and Hepatology, 4(3), 253-255. DOI Scopus4 WoS6 Europe PMC1
2009	Morona, R., Purins, L., Tocilj, A., Matte, A., & Cygler, M. (2009). Sequence-structure relationships in polysaccharide co-polymerase (PCP) proteins. Trends in Biochemical Sciences, 34(2), 78-84. DOI Scopus78 WoS66 Europe PMC52
2009	Attridge, S., Wallerstrom, G., Li, B., Morona, R., & Holmgren, J. (2009). Differential immunogenicity of Vibrio cholerae O139 variants expressing different combinations of naturally occurring and atypical forms of the serogroup polysaccharide. Vaccine, 27(7), 1055-1061. DOI Scopus4 WoS4 Europe PMC4
2008	May, K., & Morona, R. (2008). Mutagenesis of the Shigella flexneri autotransporter IcsA reveals novel functional regions involved in IcsA biogenesis and recruitment of host neural Wiscott-Aldrich syndrome protein. Journal of Bacteriology, 190(13), 4666-4676. DOI Scopus39 WoS40 Europe PMC31
2008	Purins, L., Van Den Bosch, L., Richardson, V., & Morona, R. (2008). Coiled-coil regions play 4 role in the function of the Shigella flexneri O-antigen chain length regulator Wzz(pHS2). Microbiology, 154(Part 4), 1104-1116. DOI Scopus34 WoS34 Europe PMC27
2008	Tocilj, A., Munger, C., Proteau, A., Morona, R., Purins, L., Ajamian, E., . . . Cygler, M. (2008). Bacterial polysaccharide co-polymerases share a common framework for control of polymer length. Nature Structural and Molecular Biology, 15(2), 130-138. DOI Scopus103 WoS97 Europe PMC75
2006	Paton, A., Morona, R., & Paton, J. (2006). Treatment and Prevention of Enteric Infections With Toxin-binding Probiotics. Discovery Medicine, 6(31), 35-39. Scopus1
2006	Morona, J., Morona, R., & Paton, J. (2006). Attachment of capsular polysaccharide to the cell wall of Streptococcus pneumoniae type 2 is required for invasive disease. Proceedings of the National Academy of Sciences of the United States of America, 103(22), 8505-8510. DOI Scopus111 WoS98 Europe PMC83
2006	Focareta, A., Paton, J., Morona, R., Cook, J., & Paton, A. (2006). A recombinant probiotic for treatment and prevention of cholera. Gastroenterology, 130(6), 1688-1695. DOI Scopus81 WoS67 Europe PMC54
2006	Diaz, P., Slakeski, N., Reynolds, E., Morona, R., Rogers, A., & Kolenbrander, P. (2006). Role of oxyR in the oral anaerobe Porphyromonas gingivalis. Journal of Bacteriology, 188(7), 2454-2462. DOI Scopus75 WoS75 Europe PMC63
2006	Murray, G., Attridge, S., & Morona, R. (2006). Altering the length of the lipopolysaccharide O antigen has an impact on the interaction of Salmonella enterica serovar typhimurium with macrophages and complement. Journal of Bacteriology, 188(7), 2735-2739. DOI Scopus148 WoS127 Europe PMC107
2006	Paton, A., Morona, R., & Paton, J. (2006). Designer probiotics for prevention of enteric infections. Nature Reviews. Microbiology, 4(3), 193-200. DOI Scopus98 WoS79 Europe PMC49
2005	Paton, A., Jennings, M., Morona, R., Wang, H., Focareta, A., Roddam, L., & Paton, J. (2005). Recombinant probiotics for treatment and prevention of enterotoxigenic escherichia coli diarrhea. Gastroenterology, 128(5), 1219-1228. DOI Scopus88 WoS74 Europe PMC55
2005	Korres, H., Mavris, M., Morona, R., Manning, P., & Verma, N. (2005). Topological analysis of GtrA and GtrB proteins encoded by the serotype-converting cassette of Shigella flexneri. Biochemical and Biophysical Research Communications, 328(4), 1252-1260. DOI Scopus29 WoS30 Europe PMC25
2005	Murray, G., Attridge, S., & Morona, R. (2005). Inducible serum resistance in Salmonella typhimurium is dependent on wzzfepE-regulated very long O antigen chains. Microbes and Infection, 7(13), 1296-1304. DOI Scopus57 WoS52 Europe PMC44
2004	Morona, J., Miller, D., Morona, R., & Paton, J. (2004). The effect that mutations in the conserved capsular polysaccharide biosynthesis genes cpsA, cpsB, and cpsD have on virulence of Streptococcus pneumoniae. Journal of Infectious Diseases, 189(10), 1905-1913. DOI Scopus110 WoS104 Europe PMC92
2004	Casjens, S., Winn-Stapley, D., Gilcrease, E., Morona, R., Kuhlewein, C., Chua, J., . . . Clark, A. (2004). The chromosome of Shigella flexneri bacteriophage Sf6: Complete nucleotide sequence, genetic mosaicism, and DNA packaging. Journal of Molecular Biology, 339(2), 379-394. DOI Scopus111 WoS106 Europe PMC97
2004	Pinyon, R., Paton, J., Paton, A., Botten, J., & Morona, R. (2004). Refinement of a therapeutic Shiga toxin-binding probiotic for human trials. Journal of Infectious Diseases, 189(9), 1547-1555. DOI Scopus34 WoS28 Europe PMC17
2004	CASJENS, S. (2004). The Chromosome of Shigella flexneri Bacteriophage Sf6: Complete Nucleotide Sequence, Genetic Mosaicism, and DNA Packaging1. Journal of Molecular Biology, 339*(2), 379-394. DOI
2003	Morona, R., & Van Den Bosch, L. (2003). Lipopolysaccharide O antigen chains mask IcsA (VirG) in Shigella flexneri. FEMS Microbiology Letters, 221(2), 173-180. DOI Scopus31 WoS31 Europe PMC29
2003	Freiberg, A., Morona, R., Van Den Bosch, L., Jung, C., Behlke, J., Carlin, N., . . . Baxa, U. (2003). The tailspike protein of Shigella phage Sf6. A structural homolog of Salmonella phage P22 tailspike protein without sequence similarity in the β-helix domain. Journal of Biological Chemistry, 278(3), 1542-1548. DOI Scopus46 WoS44 Europe PMC35
2003	Murray, G., Attridge, S., & Morona, R. (2003). Regulation of Salmonella typhimurium lipopolysaccharide O antigen chain length is required for virulence; identification of FepE as a second Wzz. Molecular Microbiology, 47(5), 1395-1406. DOI Scopus181 WoS170 Europe PMC143
2003	Morona, R., Daniels, C., & Van Den Bosch, L. (2003). Genetic modulation of Shigella flexneri 2a lipopolysaccharide O antigen modal chain length reveals that it has been optimized for virulence. Microbiology, 149(4), 925-939. DOI Scopus97 WoS96 Europe PMC83
2003	Morona, R., & Van Den Bosch, L. (2003). Multicopy icsA is able to suppress the virulence defect caused by wzzSF mutation in the Shigella flexneri. FEMS Microbiology Letters, 221(2), 213-219. DOI Scopus10 WoS11 Europe PMC9
2003	Morona, J., Morona, R., Miller, D., & Paton, J. (2003). Mutational analysis of the carboxy-terminal (YGX)4 repeat domain of CpsD, an autophosphorylating tyrosine kinase required for capsule biosynthesis in Streptococcus pneumoniae. Journal of Bacteriology, 185(10), 3009-3019. DOI Scopus78 WoS71 Europe PMC58
2003	Van Den Bosch, L., & Morona, R. (2003). The actin-based motility defect of a Shigella flexneri rmlD rough LPS mutant is not due to loss of IcsA polarity. Microbial Pathogenesis, 35(1), 11-18. DOI Scopus24 WoS23 Europe PMC17
2002	Morona, J., Morona, R., Miller, D., & Paton, J. (2002). Streptococcus pneumoniae capsule biosynthesis protein CpsB is a novel manganese-dependent phosphotyrosine-protein phosphatase. Journal of Bacteriology, 184(2), 577-583. DOI Scopus108 WoS95 Europe PMC78
2002	Ogunniyi, A., Kotlarski, I., Morona, R., & Manning, P. (2002). Epitope analysis of the FanC subunit protein of the K99 (F5) fimbriae of enterotoxigenic Escherichia coli using a recombinant fusion technique. FEMS Immunology and Medical Microbiology, 34(1), 23-31. DOI Scopus7 WoS6 Europe PMC4
2001	Paton, J., Rogers, T., Morona, R., & Paton, A. (2001). Oral administration of formaldehyde-killed recombinant bacteria expressing a mimic of the shiga toxin receptor protects mice from fatal challenge with shiga-toxigenic Escherichia coli. Infection and Immunity, 69(3), 1389-1393. DOI Scopus43 WoS39 Europe PMC24
2001	Paton, A., Morona, R., & Paton, J. (2001). Neutralization of shiga toxins Stx1, Stx2c, and Stx2e by recombinant bacteria expressing mimics of globotriose and globotetraose. Infection and Immunity, 69(3), 1967-1970. DOI Scopus62 WoS55 Europe PMC42
2000	Morona, R., Van Den Bosch, L., & Daniels, C. (2000). Evaluation of Wzz/MPA1/MPA2 proteins based on the presence of coiled-coil regions. Microbiology, 146(1), 1-3. DOI Scopus97 WoS87 Europe PMC60
2000	Morona, J., Paton, J., Miller, D., & Morona, R. (2000). Tyrosine phosphorylation of CpsD negatively regulates capsular polysaccharide biosynthesis in Streptococcus pneumoniae. Molecular Microbiology, 35(6), 1431-1442. DOI Scopus187 WoS173 Europe PMC143
2000	Paton, A., Morona, R., & Paton, J. (2000). A new biological agent for treatment of Shiga toxigenic Escherichia coli infections and dysentery in humans. Nature Medicine, 6(3), 265-270. DOI Scopus184 WoS162 Europe PMC103
1999	Daniels, C., & Morona, R. (1999). Analysis of Shigella flexneri Wzz (Rol) function by mutagenesis and cross-linking: Wzz is able to oligomerise.. Molecular Microbiology, 34(1), 181-194. DOI Scopus87 WoS87 Europe PMC71
1999	Chua, J., Manning, P., & Morona, R. (1999). The Shigella flexneri bacteriophage Sf6 tailspike protein (TSP)/endorhamnosidase is related to the bacteriophage P22 TSP and has a motif common to exo- and endoglycanases, and C-5 epimerases. Microbiology - UK, 145(7), 1649-1659. DOI Scopus37 WoS36 Europe PMC26
1999	Baker, S., Gunn, J., & Morona, R. (1999). The Salmonella typhi melittin resistance gene pqaB affects intracellular growth in PMA-differentiated U937 cells, polymyxin B resistance, and lipopolysaccharide.. Microbiology - UK, 145(2), 367-378. DOI Scopus69 WoS66 Europe PMC51
1999	Morona, J., Miller, D., Coffey, T., Vindurampulle, C., Spratt, B., Morona, R., & Paton, J. (1999). Molecular and genetic characterization of the capsule biosynthesis locus of Streptococcus pneumoniae type 23F. Microbiology - UK, 145(4), 781-789. DOI Scopus26 WoS23 Europe PMC18
1999	Morona, J., Morona, R., & Paton, J. (1999). Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types beloning to serogroup 19. Journal of Bacteriology, 181(17), 5355-5364. DOI Scopus54 WoS51 Europe PMC43
1999	Morona, J., Morona, R., & Paton, J. (1999). Analysis of the 5' portion of the type 19A capsule locus identifies two classes of cpsC, cpsD, and cpsE genes in Streptococcus pneumoniae. Journal of Bacteriology, 181(11), 3599-3605. DOI Scopus38 WoS35 Europe PMC26
1998	Coffey, T., Enright, M., Daniels, M., Morona, J., Morona, R., Hryniewicz, W., . . . Spratt, B. (1998). Recombinational exchanges at the capsular polysaccharide biosynthetic locus lead to frequent serotype changes among natural isolates of Streptococcus pneumoniae.. Molecular Microbiology, 27(1), 73-83. DOI Scopus275 WoS249 Europe PMC199
1998	Daniels, C., Vindurampulle, C., & Morona, R. (1998). Overexpression and topology of the Shigella flexneri O-antigen polymerase (Rfc/Wzy).. Molecular Microbiology, 28(6), 1211-1222. DOI Scopus164 WoS165 Europe PMC115
1997	Van Den Bosch, L., Manning, P., & Morona, R. (1997). Regulation of O-antigen chain length is required for Shigella flexneri virulence. Molecular Microbiology, 23(4), 765-775. DOI Scopus98 WoS94 Europe PMC83
1997	Mavris, M., Manning, P., & Morona, R. (1997). Mechanism of bacteriophage SfII-mediated sertype conversion in Shigella flexneri. Molecular Microbiology, 26(5), 939-950. DOI Scopus95 WoS87 Europe PMC68
1997	Morona, J., Morona, R., & Paton, J. (1997). Characterization of the locus encoding the Streptococcus pneumoniae type 19F capsular polysaccharide biuosynthetic pathway. Molecular Microbiology, 23(4), 751-763. DOI Scopus98 WoS97 Europe PMC71
1997	Ogunniyi, A., Kotlarski, I., Morona, R., & Manning, P. (1997). Role of the SefA subunit protein of SEF14 fimbriae in the pathogenesis of Salmonella enterica serovar Enteritidis. Infection and Immunity, 65(February), 708-717.
1997	Baker, S., Daniels, C., & Morona, R. (1997). PHoP/Q regulated genes in Salmonella typhi: identification of melittin sensitive mutants. Microbial Pathogenesis, 22(3), 165-179. DOI Scopus33 WoS31 Europe PMC25
1997	Paton, J., Morona, J., & Morona, R. (1997). Characterization of the Capsular Polysaccharide Biosynthesis Locus of Streptococcus pneumoniae Type 19F. Microbial Drug Resistance-Mechanisms Epidemiology and Disease, 3(1), 89-99. DOI Scopus3 WoS5 Europe PMC4
1997	Morona, J., Morona, R., & Paton, J. (1997). Molecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B. Journal of Bacteriology, 179(15), 4953-4958. DOI Scopus36 WoS34 Europe PMC29
1997	Ogunniyi, A. D., Kotlarski, I., Morona, R., & Manning, P. A. (1997). Role of SefA subunit protein of SEF14 fimbriae in the pathogenesis of Salmonella enterica serovar enteritidis. Infection and Immunity, 65(2), 708-717. DOI Scopus42 WoS37 Europe PMC21
1995	Guildolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1995). Nucleotide sequence analysis of genes essential for production of Streptococcus pneumoniae type 19F capsular polysaccharide.. Developments in biological standardization, 85, 267-271. Scopus3 WoS3 Europe PMC3
1995	Stroeher, U. H., Karageorgos, L. E., Morona, R., & Manning, P. A. (1995). In vibrio cholerae serogroup O 1, rfaD is closely linked to the rfb operon. Gene, 155(1), 67-72. DOI Scopus16 WoS14 Europe PMC10
1995	Morona, R., Van Den Bosch, L., & Manning, P. (1995). Molecular, genetic and topological characterization of O-antigen chain length regulation in Shigella flexneri. Journal of Bacteriology, 177(4), 1059-1068. DOI Scopus161 WoS163 Europe PMC124
1995	Stroeher, U., Karageorgos, L., Morona, R., & Manning, P. (1995). In Vibrio cholerae O1 rfaD is linked closely to rfb operon. Gene, 155(1), 67-72.
1995	Stroeher, U., Karageorgos, L., Brown, M., Morona, R., & Manning, P. (1995). A putative pathway for perosamine biosynthesis is the first function encoded within the rfb region of Vibrio cholerae O1. Gene, 166(1), 33-42. DOI Scopus43 WoS43 Europe PMC32
1995	Morona, R., Karageorgos, L., Manning, P., & Stroeher, U. (1995). A putative pathway for biosynthesis of the O-antigen component, 3-deoxy-L-glycero-tetronic acid, based in the nucleotide sequence of the Vibrio cholerae O1 rfb region. Gene, 166(1), 19-31. DOI Scopus10 WoS9 Europe PMC6
1995	Macpherson, D., Manning, P., & Morona, R. (1995). Genetic analysis of rfbX in Shigella flexneri. Gene, 155(1), 9-17. DOI Scopus30 WoS31 Europe PMC18
1995	Manning, P., Karageorgos, L., & Morona, R. (1995). Putative O-antigen transport genes withing the rfbregion of Vibrio cholerae O1 are homologous to those for capsule transport. Gene, 158(1), 1-7. DOI Scopus30 WoS28 Europe PMC23
1995	Jorgensen, L., O'Neill, B., Thomas, C., Morona, R., & Middelberg, A. (1995). Release of chloramphenicol acetyl transferase from recombinant Escherichia coli by sonication and the French press. Biotechnology Techniques, 9(7), 477-480. DOI Scopus7 WoS6
1995	Morona, R., Macpherson, D., Van Den Bosch, L., Carlin, N., & Manning, P. (1995). Lipopolysaccharide with an altered O-antigen produced in Escherichia coli K-12 harbouring mutated, cloned Shigella flexneri rfb genes. Molecular Microbiology, 18(2), 209-223. DOI Scopus31 WoS30 Europe PMC21
1995	Manning, P., Morona, R., & Rohde, M. (1995). Shigella: identifying the deadly diarroea. Today's Life Science, 7(3), 54-61.
1995	Stroeher, U., Dredge, B., Morona, R., Brown, M., Karageorgos, L., Albert, M., & Manning, P. (1995). Genetic rearrangements in the rfb regions of Vibrio cholerae O1 and O139. Proceedings of the National Academy of Sciences of the United States of America, 92(22), 10374-10378. DOI Scopus88 WoS80 Europe PMC62
1994	Morona, J. K., Guidolin, A., Morona, R., Hansman, D., & Paton, J. C. (1994). Isolation, characterization, and nucleotide sequence of IS1202, an insertion sequence of Streptococcus pneumoniae. Journal of Bacteriology, 176(14), 4437-4443. DOI Scopus33 WoS39 Europe PMC24
1994	Macpherson, D. F., Manning, P. A., & Morona, R. (1994). Characterization of the dTDP‐rhamnose biosynthetic genes encoded in the rfb iocus of Shigella flexneri. Molecular Microbiology, 11(2), 281-292. DOI Scopus72 WoS82 Europe PMC55
1994	Morona, R., Morona, J. K., Considine, A., Hackett, J. A., van den Bosch, L., Beyer, L., & Attridge, S. R. (1994). Construction of K88- and K99-expressing clones of Salmonella typhimurium G30: immunogenicity following oral administration to pigs. Vaccine, 12(6), 513-517. DOI Scopus20 WoS14 Europe PMC13
1994	Morona, R., Mavris, M., Fallarino, A., & Manning, P. A. (1994). Characterization of the rfc region of Shigella flexneri. Journal of Bacteriology, 176(3), 733-747. DOI Scopus128 WoS138 Europe PMC91
1994	Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Nucleotide sequence analysis of genes essential for capsular polysaccharide biosynthesis in Streptococcus pneumoniae type 19F. Infection and Immunity, 62(12), 5384-5396. DOI Scopus114 WoS117 Europe PMC80
1993	Francis, M. S., Parker, A. F., Morona, R., & Thomas, C. J. (1993). Bacteriophage lambda as a delivery vector for Tn10-derived transposons in Xenorhabdus bovienii. Applied and Environmental Microbiology, 59(9), 3050-3055. DOI Scopus8 WoS10 Europe PMC3
1993	Paton, J. C., Morona, J. K., Harrer, S., Hansman, D., & Morona, R. (1993). Immunization of mice with Salmonella typhimurium C5 aroA expressing a genetically toxoided derivative of the pneumococcal toxin pneumolysin. Microbial Pathogenesis, 14(2), 95-102. DOI Scopus8 WoS8 Europe PMC3
1992	Stroeher, U. H., Karageorgos, L. E., Morona, R., & Manning, P. A. (1992). Serotype conversion in Vibrio cholerae O1. Proceedings of the National Academy of Sciences of the United States of America, 89(7), 2566-2570. DOI Scopus167 WoS158 Europe PMC106
1991	Morona, R., Yeadon, J., Considine, A., Morona, J. K., & Manning, P. A. (1991). Construction of plasmid vectors with a non-antibiotic selection system based on the Escherichia coli thyA<sup>+</sup> gene: application to cholera vaccine development. Gene, 107(1), 139-144. DOI Scopus39 WoS33 Europe PMC25
1991	Macpherson, D. F., Morona, R., Beger, D. W., Cheah, K., & Manning, P. A. (1991). Genetic analysis of the rfb region of Shigella flexneri encoding the Y serotype O‐antigen specificity. Molecular Microbiology, 5(6), 1491-1499. DOI Scopus37 WoS43 Europe PMC34
1991	Attridge, S. R., Dearlove, C., Beyer, L., Van den Bosch, L., Howles, A., Hackett, J., . . . Rowley, D. (1991). Characterization and immunogenicity of EX880, a Salmonella typhi Ty21a-based clone which produces Vibrio cholerae O antigen. Infection and Immunity, 59(7), 2279-2284. DOI Scopus16 WoS14 Europe PMC8
1991	Morona, R., Brown, M. H., Yeadon, J., Heuzenroeder, M. W., & Manning, P. A. (1991). Effect of lipopolysaccharide core synthesis mutations on the production of Vibrio cholerae O-antigen in Escherichia coli K-12. FEMS Microbiology Letters, 82(3), 279-285. DOI Scopus32 WoS34 Europe PMC17
1990	Tacket, C. O., Forrest, B., Morona, R., Attridge, S. R., LaBrooy, J., Tall, B. D., . . . Levine, M. M. (1990). Safety, immunogenicity, and efficacy against cholera challenge in humans of a typhoid-cholera hybrid vaccine derived from Salmonella typhi Ty21a. Infection and Immunity, 58(6), 1620-1627. DOI Scopus73 WoS74 Europe PMC53
1990	Attridge, S. R., Daniels, D., Morona, J. K., & Morona, R. (1990). Surface co-expression of Vibrio cholerae and Salmonella typhi O-antigens on Ty21a clone EX210. Microbial Pathogenesis, 8(3), 177-188. DOI Scopus18 WoS22 Europe PMC15
1990	Morona, R., Matthews, M. S., Morona, J. K., & BrownBrownBrown, M. H. (1990). Regions of the cloned Vibrio cholerae rfb genes needed to determine the Ogawa form of the O-antigen. MGG Molecular & General Genetics, 224(3), 405-412. DOI Scopus4 WoS3 Europe PMC2
1989	TACKET, C. O., FORREST, B., LOSONSKY, G., MORONA, R., ATTRIDGE, S. R., LABROOY, J., . . . LEVINE, M. M. (1989). SAFETY, IMMUNOGENICITY, AND EFFICACY OF A BIVALENT CHOLERA-TYPHOID HYBRID VACCINE. CLINICAL RESEARCH, 37(2), A442.
1989	Forrest, B. D., Labrooy, J. T., Attridoe, S. R., Boehm, G., Beyer, L., Morona, R., . . . Rowley, D. (1989). Lmmunogenicity of a candidate live oral typhoid/cholera hybrid vaccine in humans. Journal of Infectious Diseases, 159(1), 145-146. DOI Scopus36 WoS33 Europe PMC24
1988	HONE, D. M., ATTRIDGE, S. R., FORREST, B., MORONA, R., DANIELS, D., LABROOY, J. T., . . . HACKETT, J. (1988). A GALE-VIA (VI-ANTIGEN-NEGATIVE) MUTANT OF SALMONELLA-TYPHI TY2 RETAINS VIRULENCE IN HUMANS. INFECTION AND IMMUNITY, 56(5), 1326-1333. DOI WoS151 Europe PMC103
1988	Attridge, S., Hackett, J., Morona, R., & Whyte, P. (1988). Towards a live oral vaccine against enterotoxigenic Escherichia coli of swine. Vaccine, 6(5), 387-389. DOI Scopus31 WoS25 Europe PMC16
1987	Hone, D., Morona, R., Attridge, S., & Hackett, J. (1987). Contruction of defined galE mutants of Salmonella for use as vaccines. Journal of Infectious Diseases, 156(1), 167-174. DOI Scopus63 WoS66 Europe PMC49
1987	Ward, H., Morelli, G., Kamke, M., Morona, R., Yeadon, J., Hackett, J., & Manning, P. (1987). A physical map of the chromosomal region determining O-antigen biosynthesis in Vibrio cholerae 01. Gene, 55(2-3), 197-204. DOI Scopus23 WoS26 Europe PMC19
1986	Alm, R. A., Braun, G., Morona, R., & Manning, P. A. (1986). Detection of an OmpA-like protein in Vibrio cholerae. FEMS Microbiology Letters, 37(1), 99-104. DOI Scopus10 WoS13
1986	Morona, R., & Henning, U. (1986). New locus (ttr) in Escherichia coli K-12 affecting sensitivity to bacteriophage T2 and growth on oleate as the sole carbon source. Journal of Bacteriology, 168(2), 534-540. DOI Scopus20 WoS21 Europe PMC14
1985	Morona, R., Kramer, C., & Henning, U. (1985). Bacteriophage receptor area of outer membrane protein OmpA of Escherichia coli K-12. Journal of Bacteriology, 164(2), 539-543. DOI Scopus98 WoS104 Europe PMC73
1985	Morona, R., Krämer, C., & Henning, U. (1985). The nature of ompA mutants of Escherichia coli K12 exhibiting temperature-sensitive bacteriophage resistance. MGG Molecular & General Genetics, 201(2), 357-359. DOI
1985	Morona, R., Tommassen, J., & Henning, U. (1985). Demonstration of a bacteriophage receptor site on the Escherichia coli K12 outer-membrane protein OmpC by the use of a protease. European Journal of Biochemistry, 150(1), 161-169. DOI Scopus40 WoS42 Europe PMC28
1984	Morona, R., & Henning, U. (1984). Host range mutants of bacteriophage Ox2 can use two different outer membrane proteins of Escherichia coli K-12 as receptors. Journal of Bacteriology, 159(2), 579-582. DOI Scopus39 WoS41 Europe PMC30
1984	Morona, R., Klose, M., & Henning, U. (1984). Escherichia coli K-12 outer membrane protein (OmpA) as a bacteriophage receptor: Analysis of mutant genes expressing altered protein. Journal of Bacteriology, 159(2), 570-578. DOI Scopus143 WoS164 Europe PMC120
1984	Morona, R., & Reeves, P. (1984). Detection of several diisopropylfluorophosphate-binding proteins in the outer membrane of Escherichia coli K-12. FEMS Microbiology Letters, 23(2-3), 179-182. DOI Scopus3 WoS3
1983	Morona, R., Manning, P. A., & Reeves, P. (1983). Identification and characterization of the TolC protein, an outer membrane protein from Escherichia coli. Journal of Bacteriology, 153(2), 693-699. DOI Scopus48 WoS57 Europe PMC45
1982	Morona, R., & Reeves, P. (1982). A new locus, stc, which affects the phenotype of tolC mutants of Escherichia coli K-12. MGG Molecular & General Genetics, 187(2), 335-341. DOI Scopus5 WoS10
1982	Morona, R., & Reeves, P. (1982). The tolC locus of Escherichia coli affects the expression of three major outer membrane proteins. Journal of Bacteriology, 150(3), 1016-1023. DOI Scopus81 WoS110 Europe PMC87
1981	Morona, R., & Reeves, P. (1981). Molecular cloning of the tolC locus of Escherichia coli K-12 with the use of transposon Tn 10. MGG Molecular & General Genetics, 184(3), 430-433. DOI Scopus16 WoS23 Europe PMC18
1980	MORONA, R., & REEVES, P. (1980). MOLECULAR-CLONING OF THE TO1C LOCUS WITH THE AID OF THE TN10 TRANSPOSON. PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13, 91.

Book Chapters

Year	Citation
2015	Standish, A., & Morona, R. (2015). Capsule structure, synthesis, and regulation. In J. Brown, S. Hammerschmidt, & C. Orihuela (Eds.), Streptococcus Pneumoniae: Molecular Mechanisms of Host-Pathogen Interactions (pp. 169-179). United Kingdom: Elsevier. DOI Scopus3
2011	Paton, A., Morona, R., & Paton, J. (2011). Designer Probiotics and Enteric Cytoprotection. In J. Malago, J. Koninkx, & R. Marinsek Logar (Eds.), Probiotic Bacteria and Enteric Infections: Cytoprotection by Probiotic Bacteria (1 ed., pp. 429-444). Netherlands: Springer. DOI
2011	Paton, A. W., Morona, R., & Paton, J. C. (2011). Designer Probiotics and Enteric Cytoprotection. In Probiotic Bacteria and Enteric Infections (pp. 429-443). Springer Netherlands. DOI

Conference Items

Year	Citation
2008	Morona, J. K., Morona, R., & Paton, J. C. (2008). Comparison of Streptococcus pneumoniae Polysaccharide Polymerase Proteins Cps19aI and Cps19fI. Poster session presented at the meeting of FASEB Summer Research Conferences: Microbial Polysaccharides of Medical, Agricultural and Industrial Importance. Carefree, Arizona, USA.
2007	Morona, J. K., Morona, R., & Paton, J. C. (2007). Comparison of Streptococcus pneumoniae Polysaccharide Polymerase Proteins Cps19aI and Cps19fI. Poster session presented at the meeting of Ninth Australian Conference on Molecular Biology of Bacterial Pathogens. Lorne, Victoria, Australia.
2006	Morona, J. K., Morona, R., & Paton, J. C. (2006). Mutation of the Conserved Capsular Polysaccharide Biosynthesis Gene cpsB of Streptococcus pneumoniae D39 results in two distinct CPS phenotypes.. Poster session presented at the meeting of The 5th International Symposium on Pneumococci and Pneumococcal Diseases. Alice Springs, Northern Territory, Australia.
2000	Morona, J. K., Morona, R., Miller, D. C., & Paton, J.C.. (2000). Mutation analysis of the carboxy-terminal [YGX]4 repeat domain of CpsD, a Streptococcus pneumoniae capsule biosynthesis protein. Poster session presented at the meeting of FASEB Summer Research Conferences: Microbial Polysaccharides of Medical, Agricultural and Industrial Importance. Copper Mountain, Colorado, USA.
1999	Morona, J. K., Morona, R., & Paton, J. C. (1999). Comparative genetics of capsular polysaccharide biosynthesis in Streptococcus pneumoniae types belonging to serogroup 19. Poster session presented at the meeting of Fifth Australian Conference on Molecular Analysis of Bacterial Pathogens. Victor Harbor, SA., Australia.
1998	Paton, J. C., Morona, J. K., & Morona, R. (1998). Characterisation of the capsule biosynthesis loci of pneumococci belonging to serogroup 19. Poster session presented at the meeting of First International symposium on Pneumococci and pneumococcal Diseases. Elsinore, Denmark.
1997	Morona, J. K., Morona, R., & Paton, J. C. (1997). Molecular and genetic characterisation of the capsule biosynthesis locus of Streptococcus pneumoniae type 19B. Poster session presented at the meeting of Fourth Australian Conference on Molecular Analysis of Bacterial Pathogens. Jamberoo, NSW. Australia.
1997	Paton, J. C., Morona, J. K., & Morona, R. (1997). Molecular analysis of capsular polysaccharide biosynthesis (cps) loci of Streptococcus pneumoniae.. Poster session presented at the meeting of Microbiology Australia. Adelaide, Australia.
1994	Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Analysis of genes essential for the production of Streptococcus pneumoniae type 19F capsular polysaccharide. Poster session presented at the meeting of Am. Soc. Microbiol. Fourth Int. Conf. Streptoccocal Genet. Sante Fe, New Mexico, USA.
1994	Guidolin, A., Morona, J. K., Morona, R., Hansman, D., & Paton, J. C. (1994). Nucleotide sequence of genes essential for production of Streptococcus pneumoniae type 19F capsular polysaccharide. Poster session presented at the meeting of Australian Microbiologist. Melbourne, Australia.

Patents

Year	Citation
2015	Wallace, J., Morona, R., Polyak, S., Booker, G., Abell, A., & Wilce, M. (2015). 9108978, Novel Antimicrobial Compounds. United States of America.
2010	Paton, J., Morona, R., & Paton, A. (2010). 2004201978, Cytotoxin with a Subtilase Domain. Australia.
2006	Paton, A., Morona, R., & Paton, J. (2006). 783536, Recombinant bacterium expressing an oligosaccharide receptor mimic. Australia.
2005	Paton, A., Paton, J., & Morona, R. (2005). Pub no. WO/2005/089775, Treatment and prevention of enterotoxigenic escherichia coli diarrhoeas. Australia.

Preprint

Year	Citation
2024	Qin, J., Hong, Y., Morona, R., & Totsika, M. (2024). Tolerance Mechanisms in Polysaccharide Biosynthesis: Implications for Undecaprenol Phosphate Recycling in<i>Escherichia coli</i>and<i>Shigella flexneri</i>. DOI

B.Sc.Honours Co-ordinator

Course Co-ordinator for Infection and Immunity A III

Past Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2021 - 2024	Principal Supervisor	The Biogenesis and Homeostasis of the Shigella flexneri Cell Envelope	Doctor of Philosophy	Doctorate	Full Time	Miss Alice Ascari
2018 - 2022	Principal Supervisor	Enterobacterial Common Antigen Biosynthesis in Shigella flexneri	Doctor of Philosophy	Doctorate	Full Time	Mr Nicholas Tadeusz Maczuga
2017 - 2021	Principal Supervisor	Investigation of the Wzy O Antigen Polymerase and the Wzz Co-Polymerase Interface	Doctor of Philosophy	Doctorate	Full Time	Mr Vincenzo Leo
2016 - 2020	Principal Supervisor	Investigation of the IcsA-mediated Shigella flexneri hyper-adherence	Doctor of Philosophy	Doctorate	Full Time	Mr Jilong Qin
2014 - 2018	Principal Supervisor	Investigation of a Low Molecular Weight Protein Tyrosine Phosphatase in Streptococcus Pneumoniae	Doctor of Philosophy	Doctorate	Full Time	Miss Zuleeza Ahmad
2013 - 2015	Principal Supervisor	Polarity and Secretion of Shigella flexneri IcsA: A Classical Autotransporter	Doctor of Philosophy	Doctorate	Full Time	Mr Matthew Thomas Doyle
2011 - 2015	Principal Supervisor	Characterisation of the Shigella flexneri O Antigen Polymerase Wzy	Doctor of Philosophy	Doctorate	Full Time	Ms Pratiti Nath
2011 - 2015	Co-Supervisor	STAPHYLOCOCCUS AUREUS: STRESS RESPONSE AND ITS ROLES IN PATHOGENESIS	Doctor of Philosophy	Doctorate	Full Time	Miss Minh Giao Long Bui
2009 - 2014	Co-Supervisor	Coupling Stress Responses and Growth Pathways in Haemophilus influenzae	Doctor of Philosophy	Doctorate	Full Time	Mr Changde Donald Jiang
2009 - 2014	Principal Supervisor	Identification of host cell proteins involved in Shigella flexneri pathogenesis	Doctor of Philosophy	Doctorate	Full Time	Miss Mabel Lum
2009 - 2012	Co-Supervisor	Cell Biology of the Interaction between Listeria monocytogenes and Colpoda spp.	Doctor of Philosophy	Doctorate	Full Time	Mr Rethish Raghu Nadhanan
2008 - 2012	Co-Supervisor	The Role of CpsC in the Regulation of Streptococcus pneumoniae Capsular Polysaccharide Biosynthesis	Doctor of Philosophy	Doctorate	Full Time	Mr James Byrne
2007 - 2009	Co-Supervisor	Role of pCT0018 for Copper Homeostasis in Listeria monocytogenes Strain DRDC8	Master of Science	Master	Full Time	Miss Mei Mei Hii
2007 - 2013	Principal Supervisor	Analysis of Shigella flexneri Cell Surface Virulence Factors	Doctor of Philosophy	Doctorate	Full Time	Dr Min Teh
2007 - 2012	Co-Supervisor	The Role of YeaZ in the VBNC State of Vibrio parahaemolyticus NCTC 10884	Doctor of Philosophy	Doctorate	Full Time	Mr Shih-Hsun Chen
2006 - 2010	Principal Supervisor	Biogenesis of Shigella flexneri IcsA Protein	Doctor of Philosophy	Doctorate	Full Time	Mr Marcin Grabowicz
2005 - 2011	Principal Supervisor	Characterisation of Shigella flexneri polysaccharide co-polymerase (PCP) protein Wzz	Doctor of Philosophy	Doctorate	Full Time	Ms Magdalene Papadopoulos
2004 - 2008	Principal Supervisor	Molecular Characterisation of Shigella flexneri Outer Membrane Protease IcsP	Doctor of Philosophy	Doctorate	Part Time	Dr Elizabeth Tran
2003 - 2007	Principal Supervisor	Molecular Characterisation of Shigella flexneri IcsA and the Role Of Lipopolysaccharide O-Antigen in Actin-based Motility	Doctor of Philosophy	Doctorate	Full Time	Dr Kerrie May
2000 - 2005	Co-Supervisor	The role of wzz genes in Salmonella typhimurium virulence	Doctor of Philosophy	Doctorate	Full Time	Mr Gerald Murray

Memberships

Date	Role	Membership	Country
2017 - ongoing	Member	Fellow of the Australian Society for Microbiology (FASM)	Australia
1990 - ongoing	Member	American Society for Microbiology	United States

Email: renato.morona@adelaide.edu.au

Renato Morona

Invasive Bacterial Pathogens Laboratory

My research interests are:

PhD and Honours Projects

Other Pathogens we have Studied Include:

Shigella flexneri

Actin Based Motility

Lipopolysaccharides

Outer Membrane Proteins

Education

Research Interests

Journals

Book Chapters

Conference Items

Patents

Preprint

Past Higher Degree by Research Supervision (University of Adelaide)

Memberships

Connect With Me

External Profiles