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Dr Nicholas Eyre

Nicholas Eyre
Postdoctoral Research Officer
School of Biological Sciences
Faculty of Sciences

I am a Molecular Virologist and Senior Post-doctoral Research Fellow in the Research Centre for Infectious Diseases (RCID) at the University of Adelaide. My research over the past 10 years has focussed on multiple aspects of virus-host interactions and viral genome replication for the related (+)RNA viruses hepatitis C virus (HCV), dengue virus (DENV) and, most recently, Zika virus (ZIKV). In particular I have sought to apply sensitive and minimally invasive high-resolution imaging techniques and targeted and random manipulation of viral genomes to better understand the viral and host determinants of viral replication compartment formation, morphology and function and how they interact with viral assembly platforms.

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Dr Nicholas Eyre

I am a Molecular Virologist and Senior Post-doctoral Research Fellow in the Research Centre for Infectious Diseases (RCID) at the University of Adelaide. My research over the past 10 years has focussed on multiple aspects of virus-host interactions and viral genome replication for the related (+)RNA viruses hepatitis C virus (HCV), dengue virus (DENV) and, most recently, Zika virus (ZIKV). In particular I have sought to apply sensitive and minimally invasive high-resolution imaging techniques and targeted and random manipulation of viral genomes to better understand the viral and host determinants of viral replication compartment formation, morphology and function and how they interact with viral assembly platforms.

Eligible to supervise Masters and PhD — email supervisor to discuss availability.

My research focuses on virus-host interactions and viral genome replication for the related (+)RNA viruses hepatitis C virus (HCV), dengue virus (DENV) and, most recently, Zika virus (ZIKV). In particular I have sought to apply novel reporter virus tools, sensitive and minimally invasive high-resolution imaging techniques and targeted and random manipulation of viral genomes to better understand the viral and host determinants of viral replication compartment formation, morphology and function and how they viral replication complexes interact with viral assembly platforms. It is hoped that identification of features of viral proteins and virus-host interactions that are essential for the viral replication cycle will identify targets for future antiviral drug development.  

Appointments

Date Position Institution name
2007 Postdoctoral Research Fellow University of Adelaide

Education

Date Institution name Country Title
2003 - 2007 University of Adelaide Australia Ph.D. (Microbiology and Immunology)
2002 - 2018 University of Adelaide Australia B.Sc. (Honours)
1999 - 2001 University of Adelaide Australia B.Sc. (Biomedical Science)

Research Interests

Journals

Year Citation
2017 Eyre, N., Aloia, A., Joyce, M., Chulanetra, M., Tyrrell, L., & Beard, M. (2017). Sensitive luminescent reporter viruses reveal appreciable release of hepatitis C virus NS5A protein into the extracellular environment. Virology, 507, 20-31.
DOI Scopus2 WoS2 Europe PMC1
2017 Van Der Hoek, K., Eyre, N., Shue, B., Khantisitthiporn, O., Glab-Ampi, K., Carr, J., . . . Beard, M. (2017). Viperin is an important host restriction factor in control of Zika virus infection. Scientific Reports, 7(1), 4475 -1-4475-14.
DOI Scopus19 WoS18 Europe PMC13
2017 Eyre, N., Johnson, S., Eltahla, A., Aloi, M., Aloia, A., McDevitt, C., . . . Beard, M. (2017). Genome-wide mutagenesis of dengue virus reveals plasticity of the NS1 protein and enables generation of infectious tagged reporter viruses. Journal of Virology, 91(23), e01455-17-1-e01455-17-25.
DOI Scopus3 WoS3 Europe PMC3
2016 Eyre, N., Hampton-Smith, R., Aloia, A., Eddes, J., Simpson, K., Hoffmann, P., & Beard, M. (2016). Phosphorylation of NS5A Serine-235 is essential to hepatitis C virus RNA replication and normal replication compartment formation. Virology, 491, 27-44.
DOI Scopus13 WoS13 Europe PMC12
2016 Fiches, G., Eyre, N., Aloia, A., Van Der Hoek, K., Betz-Stablein, B., Luciani, F., . . . Beard, M. (2016). HCV RNA traffic and association with NS5A in living cells. Virology, 493, 60-74.
DOI Scopus5 WoS5 Europe PMC5
2016 Awan, Z., Tay, E., Eyre, N., Wu, L., Beard, M., Boo, I., . . . Douglas, M. (2016). Calsyntenin-1 mediates hepatitis C virus replication. Journal of General Virology, 97(8), 1877-1887.
DOI
2016 Garbacz, W., Lu, P., Miller, T., Poloyac, S., Eyre, N., Mayrhofer, G., . . . Xie, W. (2016). Hepatic overexpression of CD36 improves glycogen homeostasis and attenuates high-fat diet-induced hepatic steatosis and insulin resistance. Molecular and Cellular Biology, 36(21), 2715-2727.
DOI Scopus11 WoS11 Europe PMC11
2015 Narayana, S., Helbig, K., McCartney, E., Eyre, N., Bull, R., Eltahla, A., . . . Beard, M. (2015). The interferon-induced transmembrane proteins, IFITM1, IFITM2 and IFITM3 inhibit hepatitis C virus entry. Journal of Biological Chemistry, 290(43), 25946-25959.
DOI Scopus29 WoS29 Europe PMC24
2015 Aloia, A., Eyre, N., Black, S., Bent, S., Gaeguta, A., Guo, Z., . . . Beard, M. (2015). Generation of a chimeric hepatitis C replicon encoding a genotype-6a NS3 protease and assessment of boceprevir (SCH503034) sensitivity and drug-associated mutations.. Antiviral therapy, 20(3), 271-280.
DOI Scopus2 WoS2 Europe PMC3
2014 Eyre, N., Fiches, G., Aloia, A., Helbig, K., McCartney, E., McErlean, C., . . . Beard, M. (2014). Dynamic imaging of the hepatitis C virus NS5A protein during a productive infection. Journal of Virology, 88(7), 3636-3652.
DOI Scopus31 WoS28 Europe PMC23
2014 Beard, M., Ffrench, R., Gowans, E., Helbig, K., Eyre, N., Douglas, M., . . . Drummer, H. (2014). A summary of the 20th International Symposium on Hepatitis C Virus and Related Viruses. Gastroenterology, 147(1), e1-e4.
DOI Scopus1
2014 Eyre, N., & Beard, M. (2014). HCV NS5A inhibitors disrupt replication factory formation: a novel mechanism of antiviral action. Gastroenterology, 147(5), 959-962.
DOI Scopus9 Europe PMC6
2014 Eyre, N. S., & Beard, M. R. (2014). A mechanism for HCV NS5A-directed antiviral therapy. Gastroenterology, 147(5), 946-947.
DOI WoS3
2014 Eyre, N. S., Helbig, K. J., & Beard, M. R. (2014). Current and future targets of antiviral therapy in the hepatitis C virus life cycle. Future Virology, 9(11), 947-965.
DOI Scopus3 WoS3
2013 McCartney, E., Helbig, K., Narayana, S., Eyre, N., Aloia, A., & Beard, M. (2013). Signal transducer and activator of transcription 3 is a proviral host factor for hepatitis C virus. Hepatology, 58(5), 1558-1568.
DOI Scopus33 WoS30 Europe PMC26
2013 Helbig, K., Carr, J., Calvert, J., Wati, S., Clarke, J., Eyre, N., . . . Beard, M. (2013). Viperin is induced following dengue virus type-2 (DENV-2) infection and has anti-viral actions requiring the C-terminal end of viperin. PLoS Neglected Tropical Diseases, 7(4), 1-14.
DOI Scopus55 WoS45 Europe PMC38
2011 Helbig, K., Eyre, N., Yip, L., Narayana, S., Li, K., Fiches, G., . . . Beard, M. (2011). The antiviral protein viperin inhibits hepatitis C virus replication via interaction with nonstructural protein 5A. Hepatology, 54(5), 1506-1517.
DOI Scopus100 WoS95 Europe PMC83
2011 McCartney, E., Eyre, N., & Beard, M. (2011). Border patrol intensifies for hepatitis C virus entry. Hepatology, 54(4), 1472-1475.
DOI Scopus2 Europe PMC1
2010 Eyre, N., Drummer, H., & Beard, M. (2010). The SR-BI partner PDZK1 facilitates Hepatitis C virus entry. PLoS Pathogens, 6(10), 1-14.
DOI Scopus32 WoS26 Europe PMC19
2010 Eyre, N., & Beard, M. (2010). Hepatitis C virus infection in living color. Hepatology, 51(5), 1852-1855.
DOI Scopus1 Europe PMC1
2009 Eyre, N., Baumert, T., & Beard, M. (2009). Closing the gap: The tight junction protein occludin and hepatitis C virus entry. Hepatology, 49(5), 1770-1772.
DOI Scopus1 Europe PMC1
2009 Eyre, N., Phillips, R., Bowden, S., Yip, L., Dewar, B., Locarnini, S., & Beard, M. (2009). Hepatitis B virus and hepatitis C virus interaction in Huh-7 cells. Journal of Hepatology, 51(3), 446-457.
DOI Scopus50 WoS44 Europe PMC33
2008 Eyre, N., Cleland, L., & Mayrhofer, G. (2008). FAT/CD36 expression alone is insufficient to enhance cellular uptake of oleate. Biochemical and Biophysical Research Communications, 370(3), 404-409.
DOI Scopus15 WoS14 Europe PMC11
2008 Helbig, K., Yip, L., McCartney, E., Eyre, N., & Beard, M. (2008). A screening method for identifying disruptions in interferon signaling reveals HCVNS3/4a disrupts Stat-1 phosphorylation. Antiviral Research, 77(3), 169-176.
DOI Scopus12 WoS11 Europe PMC11
2007 Eyre, N., Cleland, L., Tandon, N., & Mayrhofer, G. (2007). Importance of the carboxyl terminus of FAT/CD36 for plasma membrane localization and function in long-chain fatty acid uptake. Journal of Lipid Research, 48(3), 528-542.
DOI Scopus29 WoS28 Europe PMC19

Book Chapters

Conference Papers

Year Citation
2010 Eyre, N. S., Turville, S., & Beard, M. (2010). LIVE CELL IMAGING OF HEPATITIS C VIRUS NS5A TRAFFIC DURING AN INFECTIOUS LIFE CYCLE. In HEPATOLOGY Vol. 52 (pp. 376A-377A). Boston, MA: WILEY-BLACKWELL.

Conference Items

Year Citation
2013 Muller, K. R., Eyre, N. S., Van der Hoek, K. H., Li, K., & Beard, M. R. (2013). The effect of hepatitis C virus-infected Huh-7 cells on 'bystander' cells. Poster session presented at the meeting of Journal of Gastroenterology and Hepatology. Melbourne, Vic.: Wiley.
DOI
2012 Aloia, A. L., Carr, J., Eyre, N. S., Locarnini, S., Lemon, S., & Beard, M. R. (2012). Development of a genotype 6a/1b chimeric replicon for testing of direct acting antivirals against the HCV protease. Poster session presented at the meeting of HEPATOLOGY. Boston, MA: WILEY-BLACKWELL.

2017: Robinson Research Institute (RRI) Innovation Seed Funding Program. Janokovic-Karasoulos T, Van der Hoek K, Jolly LA, Eyre NS. The impact of Zika virus infection on placenta and neural development during early gestation. $25,000.

2015: ARC Linkage Infrastructure, Equipment and Facilities (LIEF) LE150100163. Gaus K, Gooding J, Boecking T, Lee L, Whisstock J, Rossjohn J, Hertzog P, Heath W, Godfrey D, Hatters D, Quiney H, Abbey B, Braet F, King N, Grau G, van Oijen A, Goldys E, Mak J, Yap A, Meunier F, Eyre N, Russell S. Single molecule imaging laboratory. $560,000.

2015-2016: Promega – Technology Development Collaboration. Development of NanoBRET assays to measure protein-nucleic acid interactions. Eyre NS (CIA). $30,000 (in kind).

2013-2015: Australian Centre for HIV and Hepatitis Virology Research (ACH2) Expression of Interest Project Grant 2013-22 . Eyre NS (CIA), Aloia AL. Hepatitis C virus NS5A protein phosphorylation as a target for antiviral drug development. $120,000.

2012-2014: NHMRC Project Grant APP1027641. Beard MR, Eyre NS (CIB), Turville SG. Imaging the hepatitis C virus lifecycle in real-time. $461,325.

2011: Royal Adelaide Hospital Clinical Project Grant. Eyre NS (CIA; sole investigator). Isolation of a genotype 1 hepatitis C virus clone that can be efficiently propagated in cell culture. $25,000.

2011: School of Molecular and Biomedical Science (SMBS) Internal Funding Scheme Grant. Beard MR, Eyre NS (CIB). Understanding host viral interactions through live imaging of the HCV NS5A and core proteins. $22,500.

2008: Royal Adelaide Hospital Clinical Project Grant. Eyre NS (CIA; sole investigator). The involvement of PDZK1 in SR-BI-dependent entry of hepatitis C virus. $20,000.

2014-present:   Lecturer, Infection and Immunity (2nd year), University of Adelaide (2 lectures/year).

2014-present:   Lecturer, Infection and Immunity (3rd year), University of Adelaide (2 lectures/year).

2014-present:   Practical demonstrator, Microbiology and Immunology (3rd year), University of Adelaide (6 practicals/year).

2011-present:   Lecturer, Biomedical Science II (2nd year), University of Adelaide (2 lectures/year).

2012-2015:      Lecturer, Masters in Biotechnology, University of Adelaide (1 lecture/year).

2012-2015:      Tutor, Microbiology and Immunology (2nd year), University of Adelaide (3 tutorials/year).

2007-2008:      Tutor, Microbiology and Immunology (2nd year), University of Adelaide (3 tutorials/year).

2004-2005:      Tutor, Microbiology and Immunology (2nd year), University of Adelaide (8 tutorials/year).

2005:               Practical demonstrator, Microbiology and Immunology (3rd year), University of Adelaide (5 practicals/year).

2004-2005:      Practical demonstrator, School of Medicine (Microbiology component, 2nd year), University of Adelaide (7 practicals/year).

2004-2005:      Practical demonstrator, Engineering (Microbiology component, 2nd year), University of Adelaide (7 practicals/year).

Current Higher Degree by Research Supervision (University of Adelaide)

Date Role Research Topic Program Degree Type Student Load Student Name
2018 Co-Supervisor Characterising the molecular interactions of flaviviruses with the type-I Interferon response Doctor of Philosophy Doctorate Full Time Ms Rosa Cedar Coldbeck-Shackley
2017 Co-Supervisor Innate Immunity to Hepatitis B in a Liver Organoid Culture System Doctor of Philosophy Doctorate Full Time Dr Chuan Kok Lim
2016 Co-Supervisor Understanding the Interactions between Pathogens and the Host Immune Response Doctor of Philosophy Doctorate Full Time Mr Colt Wesley Nash

Past Higher Degree by Research Supervision (University of Adelaide)

Date Role Research Topic Program Degree Type Student Load Student Name
2014 - 2015 Co-Supervisor The Bystander Effect in Hepatitis C Virus Infection: Cellular Interactions Between Infected Cells and Uninfected Cells Doctor of Philosophy Doctorate Full Time Ms Kate Rebecca Muller

Memberships

Date Role Membership Country
2017 - ongoing Treasurer Australian Centre for Hepatitis Virology (ACHV) Australia
Position
Postdoctoral Research Officer
Phone
83135399
Fax
8313 7532
Campus
North Terrace
Building
Molecular Life Sciences, floor 3
Room Number
3 34
Org Unit
Molecular and Biomedical Science

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