School of Biological Sciences
Faculty of Sciences
Eligible to supervise Masters and PhD - email supervisor to discuss availability.
Project #1. Precision medicine for mental health.
Approximately half of every population have natural gene variations that affect many of the medicines we use. Identifying the genetic variations for an individual can guide the dose or type of medicines prescribed. Matching of pharmaceuticals to particular genotypes is known as pharmacogenomics. It is estimated ~50% of patients do not respond to the first antidepressant they are prescribed. Access to accurate genetic testing, interpretation and translation into precision medicine is required to increase successful treatment responses.
A number of genetic analysis platforms are used to identify gene variations including microarrays, high resolution melt analysis, sanger-, second- and third- generation sequencing. The suitability of each analysis platform is dependent on the gene(s) of interest. Our laboratory is interested in long-read third generation sequencing technologies to better detect variations in genes with high homology to other genes. Variants are read from the sequence data, converted into allele types, genotypes and functional phenotypes.
We aim to improve genotyping for precision medicine in mental health services. This project offers individuals genetic testing to recommend precision medicine to guide clinical pharmacologists and prescribing physicians. Personalised prescriptions are particularly important for antidepressants and antipsychotics to decrease the length of time to optimise treatments. Our research team includes a neuroscientist and geneticist, psychiatric clinician, clinical pharmacologist and psychiatrists.
Project #2. Epigenetics and Major Depression.
Chronic psychological stress often manifests itself as Major Depressive Disorder (MDD). Sustained stress can alter corticotrophin releasing hormone, adrenocorticotropic hormone and cortisol. The homeostasis of the hypothalamic–pituitary–adrenal (HPA) axis can become dysregulated by a number of maladaptive changes when this neuroendocrine system is kept under pressure.
Stressors in our environment have an impact on gene expression through epigenetic modifications to our chromosomes. One type of epigenetic change is the modification of histone proteins that pack our DNA into chromosomes. A regulator of gene expression PHF21B is associated with major depression. PHF21B belongs to a class of proteins that read epigenetic modifications on histones and respond by facilitating specific changes to gene expression. Our aim is to understand what cell signals regulate PHF21B and determine the set of gene expression changes mediated by this epigenetic reader.
Project # 3. Discovery of MECFS Biomarkers.
Myalgic encephalomyelitis chronic fatigue syndrome (MECFS) often develops following a viral infection and can disrupt usual life activities and sleep. Suffers of this long-term illness can develop depression in time, although MECFS is distinctly different from depression. While exercise may exacerbate fatigue in MECSF suffers, those with depression often report exercise can improved mood. Similarly, antidepressants are not likely to give symptom relief for those with MECSF.
In collaboration with Dr Michael Musker our research investigates potential genetic and inflammatory biomarkers in MECFS suffers. South Australian study participants include those with MECFS and a reference group without MECFS. Our preliminary study examined circadian expression levels of selected cytokines in peripheral blood in conjunction with medical biochemistry, and a range of other measures. Participants are currently being recruited for the next two phases of the study.
Year Citation 2015 Wong, M., Lewis, M., & Licinio, J. (2015). Translational research in endocrinology and neuroimmunology applied to depression. In N. Vale (Ed.), Biomedical Chemistry: Current Trends and Developments (pp. 119-131).
Year Citation 2019 Licinio, J., Inserra, A., Choo, J., Lewis, M., Rogers, G., & Wong, M. (2019). Deficiency in caspase 1, interferon-gamma receptor and nitric oxide synthase 2 alters depressive-, anxiety-like behaviour and gut microbiota. In EUROPEAN NEUROPSYCHOPHARMACOLOGY Vol. 29 (pp. S22-S23). Copenhagen, DENMARK: ELSEVIER.
2018 Doig, R. O., Lewis, M., Clark, J., Keller, M., Jones, C., & Freeman, B. (2018). 18-KDA TRANSLOCATOR PROTEIN RADIOLIGAND [18F]GE-180 AS A DIAGNOSTIC AND PROGNOSTIC BIOMARKER FOLLOWING SPINAL CORD INJURY. In JOURNAL OF NEUROTRAUMA Vol. 35 (pp. A46). Toronto, CANADA: MARY ANN LIEBERT, INC. 2017 Licinio, J., Arcos-Burgos, M., Liu, S., Velez, J., Baune, B., Jawahar, C., . . . Wong, M. -L. (2017). Major Depression is Associated With Rare Variants in the PHF21B Gene, a Modulator of the Stress Response in Rodents. In NEUROPSYCHOPHARMACOLOGY Vol. 42 (pp. S549-S550). Palm Springs, CA: NATURE PUBLISHING GROUP. 2016 Wong, M. -L., Lee, S., Dutcher, E., Lewis, M., Mastronardi, C., & Licinio, J. (2016). Metabolic Outcomes in Response to Chronic Stress and Antidepressant Treatment. In NEUROPSYCHOPHARMACOLOGY Vol. 41 (pp. S363). NATURE PUBLISHING GROUP. 2016 Licinio, J., Inserra, A., Lewis, M., Yu, C., Mastronardi, C., Rogers, G., . . . Wong, M. -L. (2016). Inflammasome Activation in the Brain: Impact on Depressive-Like Behaviour. In NEUROPSYCHOPHARMACOLOGY Vol. 41 (pp. S182). NATURE PUBLISHING GROUP. 2015 Lee, S., Lewis, M., Mastronardi, C. A., Li, R., Smith, P., Licinio, J., & Wong, M. -L. (2015). The Stress-Antidepressant-diet (SAD) Paradigm and Weight Gain. In NEUROPSYCHOPHARMACOLOGY Vol. 40 (pp. S308). Hollywood, FL: NATURE PUBLISHING GROUP. 2014 Tsiminis, G., Klarić, T., Schartner, E., Warren-Smith, S., Lewis, M., Koblar, S., & Monro, T. (2014). Fibre-optic photochemical stroke: generating and measuring photochemical changes inside the brain. In J. LopezHiguera, J. Jones, M. LopezAmo, & J. Santos (Eds.), Proceedings of SPIE - The International Society for Optical Engineering Vol. 9157 (pp. 9157D4-1-9157D4-4). Santander, Spain: SPIE.
Year Citation 2017 Wong, M. -L., Lee, S. H., Vincent, A., Lewis, M., Mastronardi, C., & Licinio, J. (2017). Antidepressants Restore the Long-Lasting Effects of Stress on Body Weight, and Those Effects are Associated With Leptin Levels. Poster session presented at the meeting of NEUROPSYCHOPHARMACOLOGY. Palm Springs, CA: NATURE PUBLISHING GROUP. 2015 Inserra, A., Lewis, M., Mastronardi, C., Rogers, G., Leong, L., Choo, J., . . . Licinio, J. (2015). Inflammasome Signaling Affects Anxiety- and Depressive-Like Behaviors and Gut Microbiome Composition. Poster session presented at the meeting of NEUROPSYCHOPHARMACOLOGY. Hollywood, FL: NATURE PUBLISHING GROUP.
2014 Djukic, M., Milton, A., Hamilton-Bruce, A., Lewis, M., Leung, E., Jannes, J., . . . Koblar, S. (2014). Targeted peptide quantification of candidate plasma proteins to diagnose Transient Ischaemic Attack (TIA). Poster session presented at the meeting of Abstracts of the 25th Annual Scientific Meeting of the Stroke Society of Australasia, as published in International Journal of Stroke. Hamilton Island, Qld.: Wiley-Blackwell.
2013 Leong, W., Klaric, T., Lin, Y., Koblar, S., & Lewis, M. (2013). Npas4 upregulation in the corticolimbic system in stroke: implications for post-stroke depression. Poster session presented at the meeting of INTERNATIONAL JOURNAL OF STROKE. WILEY-BLACKWELL. 2012 Koblar, S., Djukic, M., Lewis, M., Leung, E., Jannes, J., Hamilton-Bruce, M., & Chataway, T. (2012). Candidate Plasma Biomarkers for Transient Ischaemic Attack (TIA). Poster session presented at the meeting of INTERNATIONAL JOURNAL OF STROKE. WILEY-BLACKWELL.
Past Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2012 - 2015 Principal Supervisor The Role of Npas4 in Neuroprotection and Neurogenesis Doctor of Philosophy Doctorate Full Time Mr Fong Chan Choy 2010 - 2014 Co-Supervisor Neurophysiology and Electrophysiology of Human and Murine Dental Pulp Stem Cells Doctor of Philosophy Doctorate Full Time Miss Kylie Maree Ellis 2009 - 2017 Co-Supervisor Proteomic Investigations and Biomarker Discovery in Transient Ischaemic Attack Doctor of Philosophy Doctorate Full Time Mr Michael Djukic 2009 - 2012 External Supervisor Stem Cell and Molecular Investigations Following Focal Cerebral Ischemia Doctor of Philosophy Doctorate Full Time Miss Wai Khay Leong 2006 - 2012 Co-Supervisor Expression and Function of Npas4 During Early Development Doctor of Philosophy Doctorate Full Time Dr Tom Klaric
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