Associate Professor Mark Nottle

Mark Nottle
Principal Research Fellow
Adelaide Medical School
Faculty of Health and Medical Sciences

Associate Professor Mark Nottle
Research Interests:

The Reproductive Biotechnology Group has an international reputation in the general areas of reproductive biology and the development of associated technologies for biomedical and agricultural applications. In collaboration with a number of university, institute and hospital research groups in Australia as well as overseas, current research is focused in the general area of regenerative medicine in particular stem cell research, as well as the development of assisted reproductive technologies including in vitro oocyte maturation.

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Associate Professor Mark Nottle

Associate Professor Mark Nottle
Research Interests:

The Reproductive Biotechnology Group has an international reputation in the general areas of reproductive biology and the development of associated technologies for biomedical and agricultural applications. In collaboration with a number of university, institute and hospital research groups in Australia as well as overseas, current research is focused in the general area of regenerative medicine in particular stem cell research, as well as the development of assisted reproductive technologies including in vitro oocyte maturation.

Isolation  and characterisation of a new  stem cell type

Current research in the Laboratory is  focussed on characterising a new embryonic  stem cell isolated by us .This breakthrough will  impact on the development of cell based therapies for a range of diseases including Type 1 diabetes  for which no effective treatment currently exists as well as having other applications such as drug discovery . This cell type is patent protected and we have formed  a Company to commercialise   this research.

Improving Human IVF outcomes

Other research being undertaken in the Laboratory  includes developing new approaches to human IVF aimed at overcoming the need for hormonal stimulation which is around half the cost of an IVF cycle. This involves adding hormones and growth factors found in the follicle to  in vitro oocyte maturation media and examining embryo development.

Biomarkers for  overcoming early embryonic loss

Industry sponsored research includes the development of molecular selection markers for improving livestock growth  and reproductive performance. The later of  which may has  implications for overcoming early embryonic loss and recurrent miscarriage in humans.

Honours and PhD projects are available in al of these areas.

Appointments

Date Position Institution name
2016 Director ICMStemcell Pty Ltd
2015 Deputy Head, Adelaide Medical School University of Adelaide, Adelaide
2013 - 2015 Acting Head, School of Paediatrics and Reproductive Health University of Adelaide, Adelaide
2012 - 2016 Convenor, Higher Degrees by Research University of Adelaide, Adelaide
2007 - 2011 Director University of Adelaide, Adelaide
2005 NHMRC Senior Research Fellow University of Adelaide, Adelaide
2003 Associate Professor University of Adelaide, Adelaide

Awards and Achievements

Date Type Title Institution Name Amount
2015 Fellow, Society for Reproductive Biology.

Education

Date Institution name Country Title
1990 University of Adelaide, Adelaide Australia Doctor of Philosophy
1982 University of Adelaide, Adelaide Australia Bachelor of Agricultural Science Honours (First Class)
1981 University of Adelaide, Adelaide Australia Bachelor of Agricultural Science

Journals

Date Citation
2017 Highet,A, Bianco-Miotto,T, Pringle,K, Peura,A, Bent,S, Zhang,J, Nottle,M, Thompson,J, Roberts,C, 2017, A novel embryo culture media supplement that improves pregnancy rates in mice, Reproduction, 153, 3, 327-340 10.1530/REP-16-0517
2016 Langendijk,P, Chen,T, McIlfatrick,S, Nottle,M, 2016, Energy balance influences number of ovulations rather than embryo quality in the pig, Theriogenology, Online, 4, 1-6 10.1016/j.theriogenology.2016.03.029
2015 Harrison,S, Beebe,L, Vassiliev,I, McIlfatrick,S, Nottle,M, 2015, Multipotent cell types in primary fibroblast cell lines used to clone pigs using somatic cell nuclear transfer, Cloning & Transgenesis, 4, 2, 1000136-1-1000136-4 10.4172/2168-9849.1000136
2015 Nottle,MB, Hawthorne,W, O'Connell,PJ, d’Apice,AJF, Cowan,PJ, 2015, Xenotransplantation transgenesis. Are we there yet?, Cloning & Transgenesis, 4, 2, 1000137-1-1000137-2 10.4172/2168-9849.1000137
2015 Le Bas-Bernardet,S, Tillou,X, Branchereau,J, Dilek,N, Poirier,N, Châtelais,M, Charreau,B, Minault,D, Hervouet,J, Renaudin,K et al, 2015, Bortezomib, C1-inhibitor and plasma exchange do not prolong the survival of multi-transgenic GalT-KO pig kidney xenografts in baboons, American Journal of Transplantation, 15, 2, 358-370 10.1111/ajt.12988
2015 Stewart,J, Tarantal,A, Hawthorne,W, Salvaris,E, O'Connell,P, Nottle,M, d'Apice,A, Cowan,P, Kearns-Jonker,M, 2015, Clonidine inhibits anti-non-Gal IgM xenoantibody elicited in multiple pig-to-primate models, Xenotransplantation, 22, 6, 413-426 10.1111/xen.12199
2014 Vabres,B, Le Bas-Bernardet,S, Riochet,D, Chérel,Y, Minault,D, Hervouet,J, Ducournau,Y, Moreau,A, Daguin,V, Coulon,F et al, 2014, hCTLA4-Ig transgene expression in keratocytes modulates rejection of corneal xenografts in a pig to non-human primate anterior lamellar keratoplasty model, Xenotransplantation, 21, 5, 431-443 10.1111/xen.12107
2014 Stewart,J, Tarantal,A, Hawthorne,W, Salvaris,E, O'Connell,P, Nottle,M, D'Apice,A, Cowan,P, Kearns-Jonker,M, 2014, Rhesus monkeys and baboons develop clotting factor VIII inhibitors in response to porcine endothelial cells or islets, Xenotransplantation, 21, 4, 341-352 10.1111/xen.12100
2014 Hawthorne,W, Salvaris,E, Phillips,P, Hawkes,J, Liuwantara,D, Burns,H, Barlow,H, Stewart,A, Peirce,S, Hu,M et al, 2014, Control of IBMIR in neonatal porcine islet xenotransplantation in baboons, American Journal of Transplantation, 14, 6, 1300-1309 10.1111/ajt.12722
2014 Chen,Y, Stewart,J, Gunthart,M, Hawthorne,W, Salvaris,E, O'Connell,P, Nottle,M, D'Apice,A, Cowan,P, Kearns-Jonker,M et al, 2014, Xenoantibody response to porcine islet cell transplantation using GTKO, CD55, CD59, and fucosyltransferase multiple transgenic donors, Xenotransplantation, 21, 3, 244-253 10.1111/xen.12091
2014 Vassiliev,I, Tsui,A, Xian Kang,W, McIlfatrick,S, Nottle,M, 2014, Effect of cytochalasin types on the production of heterozygous parthenogenetic porcine embryos and the isolation of putative parthenogenetic embryonic stem cells, Stem Cell Biology and Research, 1, 1, 2-1-2-7 10.7243/2054-717X-1-2
2013 Spiezia,L, Boldrin,M, Radu,C, Bulato,C, Bertini,D, Bon,M, Campello,E, Vadori,M, Galli,C, Gavasso,S et al, 2013, Thromboelastographic evaluation of coagulative profiles in pig-to-monkey kidney xenotransplantation, Xenotransplantation, 20, 2, 89-99 10.1111/xen.12024
2013 Beebe,L, McIlfatrick,S, Vassiliev,I, Nottle,M, 2013, Development of an improved porcine embryo culture medium for cloning, transgenesis and embryonic stem cell isolation, Cloning & Transgenesis, 2, 2, 1-5 10.4172/2168-9849.1000107
2013 Pintore,L, Paltrinieri,S, Vadori,M, Besenzon,F, Cavicchioli,L, De Benedictis,G, Calabrese,F, Cozzi,E, Nottle,M, Robson,S et al, 2013, Clinicopathological findings in non-human primate recipients of porcine renal xenografts: quantitative and qualitative evaluation of proteinuria, Xenotransplantation, 20, 6, 449-457 10.1111/xen.12063
2013 Naso,F, Gandaglia,A, Bottio,T, Tarzia,V, Nottle,M, d'Apice,A, Cowan,P, Cozzi,E, Galli,C, Lagutina,I et al, 2013, First quantification of alpha-Gal epitope in current glutaraldehyde-fixed heart valve bioprostheses, Xenotransplantation, 20, 4, 252-261 10.1111/xen.12044
2013 Smits,R, Luxford,B, Mitchell,M, Nottle,M, 2013, Embryo survival, but not first-parity litter size, is increased when gilts are fed diets supplemented with omega-3 fatty acids from fish oil, Animal Production Science, 53, 1, 57-66 10.1071/AN12119
2013 Westall,G, Levvey,B, Salvaris,E, Gooi,J, Marasco,S, Rosenfeldt,F, Egan,C, McEgan,R, Mennen,M, Russell,P et al, 2013, Sustained function of genetically modified porcine lungs in an ex vivo model of pulmonary xenotransplantation, Journal of Heart and Lung Transplantation, 32, 11, 1123-1130 10.1016/j.healun.2013.07.001
2013 Campbell,J, Lane,M, Vassiliev,I, Nottle,M, 2013, Use of insulin to increase epiblast cell number: towards a new approach for improving ESC isolation from human embryos, BioMed Research International, 2013, 1-7 10.1155/2013/150901
2013 Campbell,J, Lane,M, Vassiliev,I, Nottle,M, 2013, Epiblast cell number and primary embryonic stem cell colony generation are increased by culture of cleavage stage embryos in insulin, Journal of Reproduction and Development, 59, 2, 131-138 10.1262/jrd.2012-103
2012 Campbell,J, Nottle,M, Vassiliev,I, Mitchell,M, Lane,M, 2012, Insulin increases epiblast cell number of in vitro cultured mouse embryos via the PI3K/GSK3/p53 pathway, Stem Cells and Development, 21, 13, 2430-2441 10.1089/scd.2011.0598
2012 Gatford,K, Smits,R, Collins,C, De Blasio,M, Roberts,C, Nottle,M, Van Wettere,W, Kind,K, Owens,J, 2012, Maternal low-dose porcine somatotropin treatment in late gestation increases progeny weight at birth and weaning in sows but not in gilts, Journal of Animal Science, 90, 5, 1428-1435 10.2527/jas.2011-4227
2012 Wheeler,D, Joseph,M, Mahamud,S, Aurand,W, Mohler,P, Pompili,V, Dwyer,K, Nottle,M, Harrison,S, d'Apice,A et al, 2012, Transgenic swine: expression of human CD39 protects against myocardial injury, Journal of Molecular and Cellular Cardiology, 52, 5, 958-961 10.1016/j.yjmcc.2012.01.002
2011 Beebe,L, Bouwman,E, McIlfatrick,S, Nottle,M, 2011, Piglets produced from in vivo blastocysts vitrified using the Cryologic Vitrification Method (solid surface vitrification) and a sealed storage container, Theriogenology, 75, 8, 1453-1458 10.1016/j.theriogenology.2010.11.043
2011 Vassiliev,I, Vassilieva,S, Truong,K, Beebe,L, McIlfatrick,S, Harrison,S, Nottle,M, 2011, Isolation and in vitro characterization of putative porcine embryonic stem cells from cloned embryos treated with Trichostatin A, Cellular Reprogramming, 13, 3, 205-213 10.1089/cell.2010.0102
2011 Fisicaro,N, Londrigan,S, Brady,J, Salvaris,E, Nottle,M, O'Connell,P, Robson,S, d'Apice,A, Lew,A, Cowan,P et al, 2011, Versatile co-expression of graft-protective proteins using 2A-linked cassettes, Xenotransplantation, 18, 2, 121-130 10.1111/j.1399-3089.2011.00631.x
2011 Athorn,R, Stott,P, Bouwman,E, Ashman,R, O'Leary,S, Nottle,M, Langendijk,P, 2011, Direct ovarian-uterine transfer of progesterone increases embryo survival in gilts, Reproduction Fertility and Development, 23, 7, 921-928 10.1071/RD10333
2011 Campbell,J, Mitchell,M, Nottle,M, Lane,M, 2011, Development of a mouse model for studying the effect of embryo culture on embryonic stem cell derivation, Stem Cells and Development, 20, 9, 1578-1587 10.1089/scd.2010.0357
2011 Mahamud,S, Wheeler,DG, Jospeh,M, Dwyer,KM, Nottle,MB, Harrison,SJ, Robson,SC, Cowan,PJ, d'Apice,AJ, Gumina,RJ et al, 2011, A Novel Transgenic Pig Model Overexpressing Ectonucleoside Triphosphate Diphosphohydrolase 1 ( entpd-1/cd39) Demonstrates Reduced Myocardial Injury, CIRCULATION, 124, 21, -
2011 Smits,R, Luxford,B, Mitchell,M, Nottle,M, 2011, Sow litter size is increased in the subsequent parity when lactating sows are fed diets containing n-3 fatty acids from fish oil, Journal of Animal Science, 89, 9, 2731-2738 10.2527/jas.2010-3593
2010 Nottle,M, Vassiliev,I, O'Connel,P, D'Apice,A, Cowan,P, 2010, On the need for porcine embryonic stem cells to produce Gal KO pigs expressing multiple transgenes to advance xenotransplantation research, Xenotransplantation, 17, 6, 411-412 10.1111/j.1399-3089.2010.00611.x
2010 Vassiliev,I, Vassilieva,S, Beebe,L, McIlfatrick,S, Harrison,S, Nottle,M, 2010, Development of culture conditions for the isolation of pluripotent porcine embryonal outgrowths from in vitro produced and in vivo derived embryos, Journal of Reproduction and Development, 56, 5, 546-551 10.1262/jrd.09-197A
2010 Gatford,K, Smits,R, Collins,C, Argent,C, De Blasio,M, Roberts,C, Nottle,M, Kind,K, Owens,J, 2010, Maternal responses to daily maternal porcine somatotropin injections during early-mid pregnancy or early-late pregnancy in sows and gilts, Journal of Animal Science, 88, 4, 1365-1378 10.2527/jas.2009-2265
2010 Gatford,K, Grupen,C, Campbell,R, Luxford,B, Smits,R, Owens,P, Nottle,M, 2010, Reproductive responses to daily injections with porcine somatotropin before mating in gilts, Journal of Reproduction and Development, 56, 5, 540-545 10.1262/jrd.10-060T
2010 Vassiliev,I, Vassilieva,S, Beebe,L, Harrison,S, McIlfatrick,S, Nottle,M, 2010, In vitro and in vivo characterization of putative porcine embryonic stem cells, Cellular Reprogramming, 12, 2, 223-230 10.1089/cell.2009.0053
2009 Bagg,M, Nottle,M, Armstrong,D, Grupen,C, 2009, Effect of follicle size and dibutyryl cAMP on the cAMP content and gap junctional communication of porcine prepubertal cumulus-oocyte complexes during IVM, Reproduction Fertility and Development, 21, 6, 796-804 10.1071/RD08165
2009 Beebe,L, Vassiliev,I, McIlfatrick,S, Nottle,M, 2009, Adding essential amino acids at a low concentration improves the development of in vitro fertilized porcine embryos, Journal of Reproduction and Development, 55, 4, 373-377 10.1262/jrd.20176
2009 Beebe,L, McIlfatrick,S, Nottle,M, 2009, Cytochalasin B and trichostatin A treatment postactivation improves In Vitro development of porcine somatic cell nuclear transfer embryos, Cloning and Stem Cells, 11, 4, 477-482 10.1089/clo.2009.0029
2009 Gatford,K, De Blasio,M, Roberts,C, Nottle,M, Kind,K, Van Wettere,W, Smits,R, Owens,J, 2009, Responses to maternal GH or ractopamine during early-mid pregnancy are similar in primiparous and multiparous pregnant pigs, Journal of Endocrinology, 203, 1, 143-154 10.1677/JOE-09-0131
2007 Bagg,M, Nottle,M, Armstrong,D, Grupen,C, 2007, Relationship between follicle size and oocyte developmental competence in prepubertal and adult pigs, Reproduction Fertility and Development, 19, 7, 797-803 10.1071/RD07018
2007 Nottle,M, Beebe,L, Harrison,S, McIlfatrick,S, Ashman,R, O'Connell,P, Salvaris,E, Fisicaro,N, Pommey,S, Cowan,P et al, 2007, Production of homozygous α-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer, Xenotransplantation, 14, 4, 339-344 10.1111/j.1399-3089.2007.00417.x
2007 Beebe,L, McIlfatrick,S, Grupen,C, Boquest,A, Harrison,S, Faast,R, Ashman,R, Wengle,J, Hamilton,H, Nottle,M et al, 2007, A comparison of two in vitro maturation media for use with adult porcine oocytes for adult somatic cell nuclear transfer, Cloning and Stem Cells, 9, 4, 564-570 10.1089/clo.2007.0025
2007 Nottle,MB, Beebe,LF, Harrison,SJ, Mcllfattric,SM, Ashman,RJ, O'Connell,PJ, Salvaris,EJ, Fisicaro,N, Pommey,S, Cowan,PJ et al, 2007, Production of homozygous alpha-1,3-galactosyltransferase knockout pigs by breeding and somatic cell nuclear transfer, XENOTRANSPLANTATION, 14, 4, 339-344 10.1111/j.1399-3089.2007.0041.x
2007 Cowan,P, Nottle,M, d'Apice,A, 2007, Molecular regulation of xenoreactivity, Current Opinion in Organ Transplantation, 12, 1, 30-36 10.1097/MOT.0b013e328012b0bb
2007 Beebe,L, McIlfatrick,S, Nottle,M, 2007, The effect of energy substrate concentration and amino acids on the in vitro development of preimplantation porcine embryos, Cloning and Stem Cells, 9, 2, 206-215 10.1089/clo.2006.0060
2006 Bagg,M, Nottle,M, Grupen,C, Armstrong,D, 2006, Effect of dibutyryl cAMP on the cAMP content, meiotic progression, and developmental potential of in vitro matured pre-pubertal and adult pig oocytes, Molecular Reproduction and Development, 73, 10, 1326-1332 10.1002/mrd.20555
2006 O'Connell,P, Lew,A, Cowan,P, Londrigan,S, Hawthorne,W, Nottle,M, d'Apice,A, 2006, Islet xenotransplantation: progress towards a clinical therapy, Current Opinion in Organ Transplantation, 11, 2, 174-179 10.1097/01.mot.0000218932.27397.19
2006 Faast,R, Harrison,S, Beebe,L, McIlfatrick,S, Ashman,R, Nottle,M, 2006, Use of adult mesenchymal stem cells isolated from bone marrow and blood for somatic cell nuclear transfer in pigs, Cloning and Stem Cells, 8, 3, 166-173 10.1089/clo.2006.8.166
2005 Verma,P, Nottle,M, Pralong,D, 2005, Nuclear transfer to oocytes and ES cells, CHROMOSOME RESEARCH, 13, 10-11
2004 Nagashima,H, Giannakis,C, Ashman,R, Nottle,M, 2004, Sex differentiation and germ cell production in chimeric pigs produced by inner cell mass injection into blastocysts, Biology of Reproduction, 70, 3, 702-707 10.1095/biolreprod.103.022681
2004 Nottle,M, Boquest,A, Harrison,S, Grupen,C, Faast,R, Ashman,R, McIlfatrick,S, 2004, Transgenic perspectives in livestock science: a review, Australian Journal of Experimental Agriculture, 44, 11, 1113-1117 10.1071/EA03237
2004 Harrison,S, Boquest,A, Grupen,C, Faast,R, Guildolin,A, Giannakis,C, Crocker,L, McIlfatrick,S, Ashman,R, Wengle,J et al, 2004, An efficient method for production α(1,3)-galactosyltransferase gene knockout pigs, Cloning and Stem Cells, 6, 4, 327-331 10.1089/clo.2004.6.327
2004 Nottle,M, Beebe,L, Harrison,S, Faast,R, Moretta,M, McIlfatrick,S, 2004, Future Directions for Mammalian Cloning, Journal of Reproduction and Development, 50, Supplement, J163-J163
2003 Cowan,P, Shinkel,T, Fisicaro,N, Godwin,J, Bernabeu,C, Almendro,N, Rius,C, Lonie,A, Nottle,M, Wigley,P et al, 2003, Targeting gene expression to endothelium in transgenic animals: a comparison of the human ICAM-2, PECAM-1 and endoglin promoters, Xenotransplantation, 10, 3, 223-231 10.1034/j.1399-3089.2003.01140.x
2003 Grupen,C, McIlfatrick,S, Ashman,R, Boquest,A, Armstrong,D, Nottle,M, 2003, Relationship between donor animal age, follicular fluid steroid content and oocyte developmental competence in the pig, Reproduction Fertility and Development, 15, 2, 81-87 10.1071/RD02086
2002 Grupen,C, Mau,J, McIlfatrick,S, Maddocks,S, Nottle,M, 2002, Effect of 6-dimethylaminopurine on electrically activated in vitro matured porcine oocytes, Molecular Reproduction and Development, 62, 3, 387-396 10.1002/mrd.10126
2002 Grupen,C, Nottle,M, Nagashima,H, 2002, Calcium release at fertilization: Artificially mimicking the oocyte's response to sperm, Journal of Reproduction and Development, 48, 4, 313-333 10.1262/jrd.48.313
2002 Harrison,S, Guidolin,A, Faast,R, Crocker,L, Giannakis,C, d'Apice,A, Nottle,M, Lyons,I, 2002, Efficient generation of [alpha](1,3) galactosyltransferase knockout porcine fetal fibroblasts for nuclear transfer, Transgenic Research, 11, 2, 143-150 10.1023/A:1015262108526
2002 Boquest,A, Grupen,C, Harrison,S, McIlfatrick,S, Ashman,R, d'Apice,A, Nottle,M, 2002, Production of cloned pigs from cultured fetal fibroblast cells, Biology of Reproduction, 66, 5, 1283-1287 10.1095/biolreprod66.5.1283
2002 Nottle,M, d'Apice,A, Cowan,P, Boquest,A, Harrison,S, Grupen,C, 2002, Transgenic perspectives in xenotransplantation, 2001, Xenotransplantation, 9, 5, 305-308 10.1034/j.1399-3089.2002.t01-1-02036.x
2002 Beebe,L, Cameron,R, Blackshaw,A, Higgins,A, Nottle,M, 2002, Piglets born from centrifuged and vitrified early and peri-hatching blastocysts, Theriogenology, 57, 9, 2155-2165 10.1016/S0093-691X(01)00720-8
2001 d'Apice,A, Nottle,M, Cowan,P, 2001, Genetic modification for xenotransplantation: Transgenics and clones, Transplantation Proceedings, 33, 7-8, 3053-3054 10.1016/S0041-1345(01)02305-3
2001 Nottle,M, Haskard,K, Verma,P, Du,Z, Grupen,C, McIlfatrick,S, Ashman,R, Harrison,S, Barlow,H, Wigley,P et al, 2001, Effect of DNA concentration on transgenesis rates in mice and pigs, Transgenic Research, 10, 6, 523-531 10.1023/A:1013007329936
2000 Cameron,R, Beebe,L, Blackshaw,A, Higgins,A, Nottle,M, 2000, Piglets born from vitrified early blastocysts using a simple technique, Australian Veterinary Journal, 78, 3, 195-196 10.1111/j.1751-0813.2000.tb10594.x
2000 Cowan,P, Cowan,A, Barlow,H, Brown,A, Chen,C, Fisicaro,N, Francis,D, Goodman,D, Han,W, Kurek,M et al, 2000, Renal xenografts from triple-transgenic pigs are not hyperacutely rejected but cause coagulopathy in non-immunosuppressed baboons, Transplantation, 69, 12, 2504-2515 10.1097/00007890-200006270-00008
2000 Verma,P, Du,Z, Crocker,L, Faast,R, Grupen,C, McIlfatrick,S, Ashman,R, Lyons,I, Nottle,M, 2000, In vitro development of porcine nuclear transfer embryos constructed using fetal fibroblasts, Molecular Reproduction and Development, 57, 3, 262-269 10.1002/1098-2795(200011)57:3<262::AID-MRD8>3.0.CO;2-X
1999 Du,Z, Verma,P, Crocker,L, Faast,R, Lyons,I, Nottle,M, 1999, Development of nuclear transfer embryos using porcine fetal fibroblasts, THERIOGENOLOGY, 51, 1, 201-201 10.1016/S0093-691X(99)91760-0
1999 Nottle,M, Verma,P, Du,Z, Grupen,C, McIlfatrick,S, Ashman,R, Chen,C, Salvaris,E, Fisicaro,N, Shinkel,T et al, 1999, Production of pigs expressing multiple transgenes for use in xenotransplantation studies, THERIOGENOLOGY, 51, 1, 422-422 10.1016/S0093-691X(99)91981-7
1999 Grupen,C, Verma,P, Du,Z, McIlfatrick,S, Ashman,R, Nottle,M, 1999, Activation of in vivo- and in vitro-derived porcine oocytes by using multiple electrical pulses, REPRODUCTION FERTILITY AND DEVELOPMENT, 11, 7-8, 457-462 10.1071/RD00033
1999 Grupen,C, Du,Z, Verma,P, McIlfatrick,S, Ashman,R, Nottle,M, 1999, Parthenogenetic development of porcine oocytes activated with multiple sets of electrical pulses, THERIOGENOLOGY, 51, 1, 356-356 10.1016/S0093-691X(99)91915-5
1999 Verma,P, Du,Z, Grupen,C, Ashman,R, McIlfatrick,S, Nottle,M, 1999, Effect of cell cycle synchronization on porcine nuclear transfer, THERIOGENOLOGY, 51, 1, 215-215 10.1016/S0093-691X(99)91774-0
1998 Nottle,M, Kleemann,D, Hocking,V, Grosser,T, Seamark,R, 1998, Development of a nutritional strategy for increasing lamb survival in Merino ewes mated in late spring/early summer, Animal Reproduction Science, 52, 3, 213-219 10.1016/S0378-4320(98)00102-X
1998 Nottle,M, Verma,P, Du,Z, Ashman,R, Grupen,C, MacIlfatrick,S, 1998, Superovulation of sows with eCG or FSH, THERIOGENOLOGY, 49, 1, 381-381 10.1016/S0093-691X(98)90734-8
1998 Cowan,P, Shinkel,T, Aminian,A, Romanella,M, Wigley,P, Lonie,A, Nottle,M, Pearse,M, d'Apice,A, 1998, High-level co-expression of complement regulators on vascular endothelium in transgenic mice: CD55 and CD59 provide greater protection from human complement-mediated injury than CD59 alone, XENOTRANSPLANTATION, 5, 3, 184-190 10.1111/j.1399-3089.1998.tb00026.x
1997 Nottle,M, Kleeman,D, Grosser,T, Seamark,R, 1997, Evaluation of a nutritional strategy to increase ovulation rate in Merino ewes mated in late spring - early summer, Animal Reproduction Science, 47, 4, 255-261 10.1016/S0378-4320(97)00025-0
1997 Nottle,M, Kleeman,D, Seamark,R, 1997, Effect of previous undernutrition on the ovulation rate of Merino ewes supplemented with lupin grain, Animal Reproduction Science, 49, 1, 29-36 10.1016/S0378-4320(97)00066-3
1997 Grupen,C, Nagashima,H, Nottle,M, 1997, Asynchronous meiotic progression in porcine oocytes matured in vitro: a cause of polyspermic fertilization?, Reproduction, Fertility and Development, 9, 2, 187-191 10.1071/R96087
1997 Nagashima,H, Ashman,R, Nottle,M, 1997, Nuclear transfer of porcine embryos using cryopreserved delipated blastomeres as donor nuclei, Molecular Reproduction and Development, 48, 3, 339-343 10.1002/(SICI)1098-2795(199711)48:3&lt;339::AID-MRD6&gt;3.0.CO;2-S
1997 Grupen,C, Nagashima,H, Nottle,M, 1997, Role of epidermal growth factor and insulin-like growth factor-I on porcine oocyte maturation and embryonic development in vitro, REPRODUCTION FERTILITY AND DEVELOPMENT, 9, 6, 571-575 10.1071/R96115
1997 Nottle,M, Nagashima,H, Verma,P, Du,Z, Grupen,C, Ashman,R, MacIlfatrick,S, 1997, Developments in transgenic techniques in pigs, Journal of Reproduction and Fertility Supplement, 52, 237-244
1996 Tearle,R, Tange,M, Zannettino,Z, Katerelos,M, Shinkel,T, vanDenderen,B, Lonie,A, Lyons,I, Nottle,M, Cox,T et al, 1996, The alpha-1,3-galactosyltransferase knockout mouse - Implications for xenotransplantation, TRANSPLANTATION, 61, 1, 13-19 10.1097/00007890-199601150-00004
1996 Kashiwazaki,N, Nagashima,H, Ashman,R, Grupen,C, Nottle,M, 1996, Freezing of in vitro derived and in vitro pre-cultured porcine blastocysts: differences of the cryoprotective effect between glycerol and ethylene glycol, Journal of Reproduction and Development, 42, 3, 193-197 10.1262/jrd.42.193
1996 Tearle,R, Tange,M, Zannettino,Z, Katerelos,M, Shinkel,T, van Denderen,B, Lonie,A, Lyons,I, Nottle,M, Cox,T et al, 1996, The alpha-1,3-galactosyltransferase knockout mouse, Transplantation, 61, 1, 13-19
1996 van Denderen,B, Pearse,M, Katerelos,M, Nottle,M, Du,Z, Aminian,A, Adam,W, Shenoy-Scaria,A, Lublin,D, Shinkel,T et al, 1996, Expression of functional decay-accelerating factor (Cd55) in transgenic mice protects against human complement-mediated attack1, Transplantation, 61, 4, 582-588 10.1097/00007890-199602270-00012
1996 Nagashima,H, Grupen,C, Ashman,R, Nottle,M, 1996, Developmental competence of in vivo and in vitro matured porcine oocytes after subzonal sperm injection, Molecular Reproduction and Development, 45, 3, 359-363 10.1002/(SICI)1098-2795(199611)45:3&lt;359::AID-MRD13&gt;3.0.CO;2-U
1995 GRUPEN,C, NAGASHIMA,H, NOTTLE,M, 1995, CYSTEAMINE ENHANCES IN-VITRO DEVELOPMENT OF PORCINE OOCYTES MATURED AND FERTILIZED IN-VITRO, BIOLOGY OF REPRODUCTION, 53, 1, 173-178 10.1095/biolreprod53.1.173
1995 Nagashima,H, Kashiwazaki,N, Ashman,R, Grupen,C, Nottle,M, 1995, Cryopreservation of porcine embryos, Nature, 374, 6521, 416-416 10.1038/374416a0
1995 Nagashima,H, Kashiwazaki,N, Ashman,R, Nottle,M, 1995, Recent advances in cryopreservation of porcine embryos, Theriogenology, 41, 113-118
1995 Nagashima,H, Kashiwazaki,N, Ashman,R, Nottle,M, 1995, Improved survival of porcine hatched blastocysts cryopreserved with glycerol and sucrose, Journal of Reproduction and Development, 41, 2, 165-170 10.1262/jrd.41.165
1995 van Dendren,B, Somerville,C, Pearse,M, Nottle,M, Du,Z, Shinkel,T, d'Apice,A, 1995, Expression of functional decay-accelerating factor in transgenic mice, Transplantation Proceedings, 27, 6, 3567-3568

Book Chapters

Date Citation
2013 Vassiliev,I, Nottle,M, 2013, Isolation and culture of porcine embryonic stem cells, Humana Press, pp. 85-95 10.1007/978-1-62703-628-3_7

Conference Papers

Date Citation
2015 Hawthorne,W, Hawkes,J, Salvaris,E, Liuwantara,D, Chew,YV, Davies,S, Barlow,H, Brady,J, Lew,A, Robson,S et al, 2015, LONG-TERM SURVIVAL OF GENETICALLY MODIFIED PORCINE NEONATAL ISLET XENOGRAFTS IN BABOONS., Joint Congress of the International-Pancreas-and-Islet-Transplantation-Association, International-Xenotransplantation-Association and Cell-Transplant-Society, Melbourne, AUSTRALIA
2015 Salvaris,E, Vassiliev,I, McIlfatrick,S, Fisicaro,N, Brady,J, Hawthrone,W, Lew,A, Nottle,M, Cowan,P, 2015, Generation of transgenic pigs expressing a T cell-depleting anti-CD2 monoclonal antibody, IPITA/IXA/CTS Joint Congress, Melbourne, AUSTRALIA
2015 Fisicaro,N, Salvaris,E, Nottle,M, Cowan,P, 2015, Targeted transgene insertion in pig cells using a high fidelity CRISPR system, IPITA/IXA/CTS Joint Congress, Melbourne, AUSTRALIA
2015 Hawthorne,W, Hawkes,J, Salvaris,E, Liuwantara,D, Chew,YV, Davies,S, Barlow,H, Brady,J, Lew,A, Robson,S et al, 2015, Long-term survival of genetically modified porcine neonatal islet xenografts in baboons, IPITA/IXA/CTS Joint Congress, Melbourne, AUSTRALIA
2015 Fisicaro,N, Salvaris,E, Nottle,M, Cowan,P, 2015, TARGETED TRANSGENE INSERTION IN PIG CELLS USING A HIGH FIDELITY CRISPR SYSTEM., Joint Congress of the International-Pancreas-and-Islet-Transplantation-Association, International-Xenotransplantation-Association and Cell-Transplant-Society, Melbourne, AUSTRALIA
2015 Salvaris,E, Vassiliev,I, McIlfatrick,S, Fisicaro,N, Brady,J, Hawthrone,W, Lew,A, Nottle,M, Cowan,P, 2015, GENERATION OF TRANSGENIC PIGS EXPRESSING A T CELL-DEPLETING ANTI-CD2 MONOCLONAL ANTIBODY., Joint Congress of the International-Pancreas-and-Islet-Transplantation-Association, International-Xenotransplantation-Association and Cell-Transplant-Society, Melbourne, AUSTRALIA
2011 Pintore,L, Paltrinieri,S, Vadori,M, Besenzon,F, Cavicchioli,L, Cozzi,E, Nottle,MB, D'Apice,AJ, Cowan,PJ, Castagnaro,M et al, 2011, Quantitative and qualitative evaluation of proteinuria in non human primates recipients of porcine xenografts, Joint International Congress of the Cell-Transplant-Society(CTS)/International-Xenotransplantation-Association (IXA), Miami, FL
2011 Salvaris,E, Fisicaro,N, Harrison,S, Nottle,M, Robson,S, d'Apice,A, Cowan,P, 2011, Expression of human thrombomodulin in GTKO/hCD55-hCD59-HTF pigs, Joint International Congress of the Cell-Transplant-Society(CTS)/International-Xenotransplantation-Association (IXA), Miami, FL
2011 Hawthorne,WJ, Phillips,P, Salvaris,E, Hawkes,J, Barlow,H, Farrell,P, Heffernan,S, Favaloro,E, Robson,SC, Lew,A et al, 2011, Gal-deficient porcine neonatal islets that express human CD55 and CD59 are protected from IBMIR in a baboon model, Joint International Congress of the Cell-Transplant-Society(CTS)/International-Xenotransplantation-Association (IXA), Miami, FL
2011 Vadori,M, Daha,M, Klar,N, Besenzon,F, Baldan,N, Cavicchioli,L, De Benedictis,GM, Boldrin,M, Innocente,V, Fante,F et al, 2011, Study of complement activation in a life supporting pig to primate xenotransplantation model using GAL KO pigs, Joint International Congress of the Cell-Transplant-Society(CTS)/International-Xenotransplantation-Association (IXA), Miami, FL
2009 Cozzi,E, Simioni,P, Nottle,M, Vadori,M, De Benedictis,G, Baldan,N, Boldrin,M, Robson,S, Besenzon,F, Cavicchioli,L et al, 2009, Preliminary study in a life supporting pig to primate xenotransplantation model using GAL KO pigs transgenic for human CD39, CD55, CD59 and fucosyltransferase, Joint Meeting of the International-Pancreas-and-Islet-Transplant-Association/International-Xenotransplanation-Association, Venice, ITALY
2009 Salvaris,E, Fisicaro,N, Harrison,S, Nottle,M, Lew,A, Robson,S, d'Apice,A, Cowan,P, 2009, Multi-gene Constructs to Accelerate the Generation of Multi-transgenic GalT knockout pigs, Joint Meeting of the International-Pancreas-and-Islet-Transplant-Association/International-Xenotransplantation-Association, Venice, ITALY
2009 Salvaris,E, Hawthorne,WJ, Peirce,S, Barlow,H, Stewart,A, Zhang,X-M, Lu,B, Crikis,S, Gock,H, Rajakumar,S et al, 2009, Improved survival of GalT KO/hCD55-hCD59 pig renal xenografts in non-immunosuppressed baboons, Joint Meeting of the International-Pancreas-and-Islet-Transplant-Association/International-Xenotransplantation-Association, Venice, ITALY
2009 Le Bas-Bernardet,S, Tillou,X, Poirier,N, Dilek,N, Leroux,S, Chatelais,M, Devalliere,J, Charreau,B, Minault,D, Hervouet,J et al, 2009, First xenotransplantations of GAL KO, CD55, CD59, CD39, HT transgenic pig kidneys in baboons, Joint Meeting of the International-Pancreas-and-Islet-Transplant-Association/International-Xenotransplanation-Association, Venice, ITALY
2007 Salvaris,E, Harrison,S, Beebe,L, Dwyer,K, Crikis,S, Hawthorne,W, Robson,S, Nottle,M, d'Apice,A, Cowan,P et al, 2007, Generation of a Gal knockout pig expressing human CD39, Joint Meeting of the International-Xenotransplantation-Association/International-Pancrcas-and-Islet-Transplant-Association/Cell-Transplant-Society, Minneapolis, MN
2007 Gatford,K, De Blasio,M, Harland,M, Roberts,C, Heinemann,G, Nottle,M, Ashman,R, Kind,K, Van Wettere,W, Pain,S et al, 2007, Effects of maternal somatotropin injections or feeding ractopamine in early-mid pregnancy in gilts and sows -UTV-AC-8/05, Manipulating Pig Production XI, Brisbane
2005 Bagg,M, Nottle,M, Armstrong,D, Grupen,C, 2005, Differences in pre-pubertal and adult oocyte developmental competence is correlated with oocyte cAMP content in the pig., 38th Annual Meeting of the Society-for-the-Study-of-Reproduction, Ctr Rech Biol Reproduct, Quebec City, CANADA
2003 Nottle,M, Beebe,L, Harrison,S, Faast,R, 2003, A review - new reproductive technologies for production and research in the pig, Manipulating Pig Production IX, Fremantle, Western Australia
2001 Nottle,M, Boquest,A, Harrison,S, Grupen,C, Tolstochev,P, Cowan,P, d'Apice,A, 2001, Xenotransplantation. A possible solution to the donor organ shortage?, Adelaide, Australia
1999 Nottle,MB, Nagashima,H, Verma,PJ, Du,ZT, Grupen,CG, McIlfatrick,SM, Ashman,RJ, Harding,MP, Giannakis,C, Wigley,PL et al, 1999, Production and analysis of transgenic pigs containing a metallothionein porcine growth hormone gene construct, Meeting on the Application of Transgenic Animals for Use in Production Agriculture, TAHOE CITY, CA

Conference Items

Date Citation
2015 Hawthorne,W, Salvaris,E, Hawkes,J, Chew,YV, Davies,S, Barlow,H, Liuwantara,D, Robson,S, Lew,A, Nottle,M et al, 2015, ENHANCED SURVIVAL OF GALT KO/HCD55-HCD59 PIG RENAL XENOGRAFTS IN BABOONS, Joint Congress of the International-Pancreas-and-Islet-Transplantation-Association, International-Xenotransplantation-Association and Cell-Transplant-Society, Melbourne, AUSTRALIA
2015 Hawthorne,W, Salvaris,E, Hawkes,J, Chew,YV, Davies,S, Barlow,H, Liuwantara,D, Robson,S, Lew,A, Nottle,M et al, 2015, Enhanced survival of GalT KO/hCD55-hCD59 pig renal xenografts in baboons, IPITA/IXA/CTS Joint Congress, Melbourne, AUSTRALIA
2014 Cowan,PJ, Hawthorne,WJ, Lew,AM, O'Connell,PJ, Nottle,MB, Robson,SC, d'Apice,AJ, 2014, Genetic modification of source pigs, 1st International Conference on Clinical Islet Xenotransplantation, Osaka, JAPAN
2013 Liuwantara,D, Phillips,P, Favaloro,E, Salvaris,E, Hawkes,J, Brady,J, Lew,A, Nottle,M, d'Apice,A, Cowan,P et al, 2013, Deciphering the mechanistic pathways of the instant blood mediated inflammatory reaction against porcine neonatal islet cells, Joint Congress of IXA and Organ Transplantation in ABO-incompatible and Hyperimmunized Recipients (IXA 2013), Osaka, Japan 10.1111/xen.12060_19
2013 Stewart,JM, Yan,C, Gunthart,M, Tarantal,AF, Lee,C, Appleby,NC, Fuentes,TI, Salvaris,EJ, Hawthorne,WJ, O'Connell,PJ et al, 2013, The application of chimerism, small molecule inhibitors, and anti-idiotypic single chain antibodies to inhibit anti-non-gal xenoantibody responses, Joint Congress of 12th International-Xenotransplantation-Association and Organ Transplantation in ABO-incompatible and Hyperimmunized Recipients, Osaka, JAPAN
2013 Vadori,M, Baldan,N, Besenzon,F, Fante,F, Boldrin,M, Cavicchioli,L, MariaDe Benedictis,G, Perota,A, Stefani,A, Calabrese,F et al, 2013, Life supporting pig-to-primate xenotransplantation model using transgenic GalT KO pigs: The Padua experience, Joint Congress of 12th International-Xenotransplantation-Association and Organ Transplantation in ABO-incompatible and Hyperimmunized Recipients, Osaka, JAPAN
2013 Hawthorne,WJ, Phillips,P, Salvaris,EJ, Hawkes,J, Liuwantara,D, Barlow,H, Robson,SC, Lew,AM, Brady,JL, Nottle,MB et al, 2013, Porcine neonatal islet cell clusters that express human CD55-CD59 with Gal KO prevent thrombosis in a pre-clinical baboon study, Joint Congress of 12th International-Xenotransplantation-Association and Organ Transplantation in ABO-incompatible and Hyperimmunized Recipients, Osaka, JAPAN

Patents

Date Citation
2010 Nottle,M; 2010; Method for the production of nuclear transfer embryos
2008 Nottle,M; 2008; Method for the production of porcine nuclear transfer embryos
2006 Boquest,A, Grupen,C, Nottle,M; 2006; Method for the production of nuclear transfer embryos
2002 Grupen,C, Nottle,M; 2002; Activation of nuclear transfer embryos
Research Funding
International
2017
 
Genetic modification to protect pig islets from T cell-mediated xenogeneic rejection Cowan PJ, Hawthorne H, Nottle MB Juvenile Diabetes Research Foundation US1.24 million
2016 Vascularised Biodegradable Temporizing Matrix as an Alternative Site for Islet Transplantation. Coates PTH, S Grey,….Nottle MB Juvenile Diabetes Research Foundation US750,000
2013-2014 Using embryonic stem cells to produce multitransgenic pigs MB Nottle Research Development Agency. South Korea US200,00
National
2016   MB Nottle BioSA  Establishment of ICMStem Pty Ltd startup 43,000
2016-2017 Development of Cytochrome b5 (CYB5A) as a selection marker for boar taint MB Nottle, Hiendleder SG. CRC for High Integrity Pork. 70,000
2015-2016 Development of the  thyroid hormone deiodinase gene DI03 as a selection marker for early embryonic loss MB Nottle. Australian Pork Limited 196,000
2014-2018 Manipulating the balance of effector and regulatory T cells to promote islet xenograft survival Cowan PJ,  Nottle MB,  Hawthorne W, Shounan Y NHMRC Project Grant 1.532 million
2013- 2017 Using porcine embryonic stem cells to advance  xenotransplantation and human stem cell research to the clinic.   National Health & Medical Research Council (NHMRC) Senior Research Fellowship 615,000
2013-2016 Towards Pig to human kidney transplantation PJ Cowan (St Vincents Hospital) MB Nottle, WJ Hawthorne (Westmead Millennium Institute). NHMRC Project Grant APP1046152 1.086 million
2012 Project I D 7A-133 Reducing early embryonic loss. MB Nottle CRC Internationally Competitive Pork Industry. 50,000
2009-2012 Preclinical studies in xenotransplantation. PJ Cowan (St Vincents Hospital Melbourne), AJF d’Apice (St Vincents Hospital Melbourne), MB Nottle. NHMRC Project Grant. 558408 1.172 million
2008-2012 Which transgenic pig will be used for islet transplantation into humans? PJ  O’Connell  (Westmead Millennium Institute), AJF d’Apice (St Vincents Hospital) , PJ Cowan (St Vincents Hospital),  AM Lew (WEHI), MB Nottle, SJ Robson  (Harvard Medical School, Boston, USA). NHMRC/JDRF special program grant in Type 1 Diabetes 447718 3 million
Teaching
Undergraduate teaching
2016 - present Lectured on various aspects of reproductive physiology in Comparative Reproductive Biology III, Bachelor of Health Sciences,  Faculty of Health Sciences.
2012-2015 Conducted student-led  tutorials   in stem cells as part of the Masters Biotechnology (Biomedical Sciences) course in the School of Molecular Biosciences in the Faculty of Sciences.
2003 - present Lectured on various aspects of animal  biotechnology in  Animal Biotechnology III School of Animal and Veterinary Sciences, Faculty of Sciences.
1992 - present Lectured on reproductive physiology and ran student led tutorials on stem cells in Human Reproductive Health III, Bachelor of Health Sciences, Faculty of Health Sciences.
Supervision
Honours students
2017 A Saini. Use of embryokines  to improve in vitro ooycte maturation and pregnancy outcomes  Primary Supervisor
2016 S Jennings. Use of embryokines  to improve bovine  in vitro ooycte maturation and embryo production. Primary Supervisor
2013 E Walker. Effect of  thyroid hormones on in vitro oocyte maturation and embryo culture. Second Class. Discipline of Obstetrics and Gynaecology.  University of Adelaide. Primary Supervisor
2012 A Tsuji. Isolation of embryonic stem cells from heterozygous parthenotes. First Class. Discipline of Obstetrics and Gynaecology.  University of Adelaide. Primary Supervisor
2011 W Kang. Isolation of parthenogenetic  embryonic stem cells. First Class. Discipline of Obstetrics and Gynaecology.  University of Adelaide. Primary Supervisor

 

Masters and PhD candidates
2016 Simone Pickering PhD Primary Supervisor
2008-2012 J Campbell PhD. A comparative study of derivation methods of high pluripotency cell lines.  Discipline of Obstetrics and Gynaecology. University of Adelaide.  Dean of Graduate Studies commendation Primary Supervisor.
2007-2011 R Verma. PhD. Investigations into neural stem cell biology. School of Medicine. University of Adelaide. Lapsed. Co-supervisor
2006-2012 R Smits. PhD (part time). Role of fatty acids in embryo development. Discipline of Obstetrics and Gynaecology. University of Adelaide.   Primary Supervisor

.

Postdoctoral Fellows
2010-2013 Dr S O’Leary Current Position. Research Fellow Adelaide School of Medicine University of Adelaide

.

 

Committee Memberships

Date Role Committee Institution Country
2015 Member Medical School Research Education Committee University of Adelaide
2015 Member Adelaide Medical School Research Committee University of Adelaide
2015 Member Adelaide Medical School Executive University of Adelaide
2012 - 2016 Member Faculty of Health Sciences Research Committee
2012 - 2016 Member Faculty Health Sciences Research Executive
2012 - 2016 Representative Research Education Development Committee University of Adelaide
2012 - 2016 Representative Domestic Scholarships Committee University of Adelaide
2012 - 2016 Member Faculty Health Sciences Research Executive
2012 - 2016 Representative International Scholarships Committee University of Adelaide
2012 - 2014 Representative Student Matters Subcommittee University of Adelaide
2010 - 2015 Member Research Committee School of Paediatrics and Reproductive Health
2010 - 2013 Member Honours Committee, School of Paediatrics and Reproductive Health
2010 - 2012 Member ARI Commercial Opportunity Panel
2010 Representative Research and Development Committee
2009 Member JDRF The Australian Type 1 Diabetes Research Directions Workshop
2008 Member International Society of Stem Cell Research
2008 - 2011 Member Robinson Institute Management Committee
2008 Member NHMRC Workshop on the Risks and Benefits of xenotransplantation in Australia.
2004 - 2011 Member Postgraduate Committee, School of Paediatrics and Reproductive Health
1997 Member Asian Reproductive Biotechnology Society
1992 Member Australasian Pig Science Association
1992 Member International Embryo Transfer Society
1985 Member Australian Society for Reproductive Biology

Editorial Boards

Date Role Editorial Board Name Country
2000 Member Editorial Board, Reproduction and Development Japan

Consulting/Advisories

Date Institution Department Organisation Type Country
2003 Australian Research Council and the National Health & Medical Research Council
1990 Various
Position
Principal Research Fellow
Phone
83134087
Campus
North Terrace
Building
Adelaide Health and Medical Sciences Building
Room Number
WS5069.02
Org Unit
Paediatrics and Reproductive Health

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