Jus St John

Professor Jus St John

NHMRC Grant-Funded Researchr E

School of Biomedicine

Faculty of Health and Medical Sciences

Eligible to supervise Masters and PhD - email supervisor to discuss availability.


My group's research focuses on how the nuclear and mitochondrial genomes interact during development in order that the cells, tissues and organs of the resultant offspring function efficiently. To do this, we employ a number of assisted reproductive technologies that are either used clinically or are at various stages of development or validation. We are applying the knowledge we accumulate from this work to develop reproductive strategies for women that suffer from repeated failed fertilisation outcome or embryo developmental arrest; and for those who are carriers of severe mitochondrial disorders that are at risk of having affected children.

Research Focus One: Understanding the role of oocyte mitochondrial DNA (mtDNA) in development outcomes.

We undertake this area of research using porcine oocytes and generate embryos and offspring through in vitro fertilisation (IVF), nuclear transfer (NT) cytoplasmic transfer (CT), and mitochondrial supplementation (mICSI). This enables us to determine how the nuclear and mitochondrial genomes interact following fertilisation and at different stages during  development; and how mtDNA segregation, transmission and replication are normally regulated, as is the case following IVF, and perturbed as these mechanisms are violated by NT and CT.

 

We are currently funded by the NHMRC (Development Grant) to generate pig models using a new assisted reproductive technology that we have been developing, which involves supplementing oocytes deficient in mtDNA with genetically identical populations of mtDNA. This technology is known as mICSI, and our published data suggest that mICSI enhances embryo quality and overcomes the predisposition of poor quality oocytes to give rise to diabetes and obesity. The grant will determine whether supplementing eggs at the time of fertilisation with genetically identical mtDNA is safe practice. Furthermore, we are also funded through an NHMRC Project Grant to determine the mechanisms associated with mICSI. 

 

We have also previously generated a derivation of another assisted reproductive technology, namely cloning or somatic cell nuclear transfer (SCNT), that ensures cloned embryos inherit their mtDNA from the population present in the oocyte only, as is the case following natural conception, and not from the somatic cell as well. This technology, known as mito-SCNT, improves embryo quality and developmental outcomes and overcomes the problem of embryos and offspring inheriting two populations of mtDNA. The outcomes from his work have allowed us to commence work on Mitochondrial Donation, a derivative of NT, that has been proposed as an assisted reproductive technology to prevent offspring from inheriting mtDNA disease. Again, we are undertaking this work in a pig model, which is funded by an NHMRC Ideas Grant.

 

Research Focus Two: Defining the role of the mtDNA set point in early development.

In recent years, we have discovered the ‘mtDNA set point’, which is a key developmental milestone that naïve (pluripotent) cells must acquire in order to initiate and complete their differentiation into mature cell types. The mtDNA set point is characterised by cells expressing pluripotent markers and having very low mtDNA copy number, which are both regulated by the epigenetic status of the nuclear genome. Furthermore, we have shown that key nuclear-encoded mtDNA replication factors are DNA methylated in a tissue specific manner that accounts for cell-specific mtDNA copy number, which requires the establishment of the mtDNA set point early during development. Our studies have also shown that failure to establish the mtDNA set point results in developmental failure and is a key characteristic of tumour-initiating cells. In addition, by depleting tumour-initiating cells of their mtDNA, we have shown that the incidence of tumour formation is significantly reduced (glioblastoma mutiforme and osteosarcoma) or prevented (multiple myeloma), as these cells re-establish the mtDNA set point and undergo differentiation and, therefore, do not form tumours.

 

We use and derive embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and tumour-initiating cell lines and tumours to perform these studies. These models allow us to focus on molecular mechanisms and to identify the specific windows during differentiation/development when these events manifest and then to confirm and characterise these events through, for example, targeted gene knockdown and overexpression.

 

Research Focus Three: Determining how mtDNA haplotypes influence phenotype. 

Recent outcomes from our research program have shown that mtDNA halpotypes influence livestock phenotypes, such as meat quality and lifetime daily gains in pigs, and reproductive efficiencies in pigs and cattle. Using stem cells models, we have been able to demonstrate that mtDNA haplotypes induce phenotypic changes through modulation of DNA methylation patterns that, in turn, affect chromosomal gene expression patterns. Using tumour cells, we have also demonstrated differences in the onset and progression of tumours based on mtDNA haplotype alone.

CURRENT RESEARCH PROJECTS AVAILABLE FOR STUDENTS:

RESEARCH PROJECT 1: Identifying variants in the mitochondrial genome predictive of oocyte quality.

Project description: A number of mitochondrial DNA variants have been identified in oocytes. However, their affect and role have yet to be properly defined, especially in oocytes from offspring derived from assisted reproductive technologies that perturb the maternal only transmission of mitochondrial DNA. Using next generation sequencing and bioinformatics tools, this project will focus on identifying variants in the mitochondrial genome that are predictive of oocyte quality and whether an oocyte will progress to fertilise and develop into an embryo. Using an experimental model of pig oocyte in vitro maturation, you will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitro fertilisation protocols. After preparing fertilised and failed to fertilise oocyte samples for next generation mitochondrial DNA sequencing technology, you will learn to apply bioinformatics tools to identify and quantify variants that are associated with good and poor oocyte quality. These outputs will then be tested in: i) a larger cohort of oocytes from commercial breeding populations and matched to data associated with economic breeding values; and ii) oocytes derived from offspring that were generated from assisted reproductive technologies that result in two populations of mitochondrial DNA being transmitted.

Projects available for:Honours / HDR / Masters / Mphil / PhD

Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Levels 5 and 6.

Research project start: Semester 1 and 2

Special requirements: Vaccination required.

 

RESEARCH PROJECT 2: Identifying global DNA methylation patterns associated in the preimplantation embryo.

Project description: With the introduction of more invasive assisted reproductive technologies into animal breeding programs and their potential implementation into human clinical IVF, we need to understand how and when they modify the epigenome during early development, especially technologies that perturb the balance between the nuclear and mitochondrial genomes. DNA methylation is a key epigenetic mechanism that regulates gene expression during early development.  Using a porcine oocyte and embryo in vitro model, next generation bisulfite DNA sequencing and bioinformatics tools, this project will focus on mapping the DNA methylation profiles of oocytes and early embryos to define the waves of DNA methylation prior to and post-embryonic genome activation, the time point when the early embryo initiates its own global transcription. You will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitro fertilisation protocols. After preparing fertilised oocyte and embryo samples for next generation bisulfite DNA sequencing, you will learn to apply bioinformatics tools to map regions of DNA methylation across the whole genome and validate the findings. These outputs will then be tested against embryos generated from other assisted reproductive technologies to determine how these technologies impact on the DNA methylation profiles of the developing embryo.

Projects available for: Third Year / Honours / HDR / Masters / Mphil / PhD

Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Levels 5 and 6.

Research project start: Semester 1 and 2

Special requirements: Vaccination required.

  • Journals

    Year Citation
    2023 McIlfatrick, S., O'Leary, S., Okada, T., Penn, A., Nguyen, V. H. T., McKenny, L., . . . St. John, J. C. (2023). Does supplementation of oocytes with additional mtDNA influence developmental outcome?. iScience, 26(2), 105956-1-105956-27.
    DOI Scopus1 WoS1 Europe PMC1
    2023 Okada, T., McIlfatrick, S., & St. John, J. C. (2023). Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts. International Journal of Molecular Sciences, 24(4), 1-26.
    DOI Scopus2 WoS2 Europe PMC1
    2023 Okada, T., Penn, A., & St. John, J. C. (2023). Mitochondrial DNA Supplementation of Oocytes Has Downstream Effects on the Transcriptional Profiles of Sus scrofa Adult Tissues with High mtDNA Copy Number. International Journal of Molecular Sciences, 24(8), 7545.
    DOI
    2022 Okada, T., Sun, X., McIlfatrick, S., & St. John, J. C. (2022). Low guanine content and biased nucleotide distribution in vertebrate mtDNA can cause overestimation of non-CpG methylation. NAR Genomics and Bioinformatics, 4(1), 1-17.
    DOI Scopus1
    2022 Azumah, R., Hummitzsch, K., Hartanti, M. D., St John, J. C., Anderson, R. A., & Rodgers, R. J. (2022). Analysis of upstream regulators, networks, and pathways associated with the expression patterns of Polycystic Ovary Syndrome candidate genes during fetal ovary development. Frontiers in Genetics, 12, 762177-1-762177-16.
    DOI Scopus4 WoS5 Europe PMC4
    2022 Okada, T., McIlfatrick, S., Hin, N., Aryamanesh, N., Breen, J., & St John, J. C. (2022). Mitochondrial supplementation of Sus scrofa metaphase II oocytes alters DNA methylation and gene expression profiles of blastocysts. Epigenetics and Chromatin, 15(1), 20 pages.
    DOI Scopus7 WoS7 Europe PMC4
    2022 Towarnicki, S. G., Youngson, N. A., Corley, S. M., St John, J. C., Melvin, R. G., Turner, N., . . . Ballard, J. W. O. (2022). Ancestral dietary change alters the development of Drosophila larvae through MAPK signalling. Fly, 16(1), 299-311.
    DOI Scopus2 WoS1 Europe PMC2
    2022 St John, J. C., Okada, T., Andreas, E., & Penn, A. (2022). The role of mtDNA in oocyte quality and embryo development. Molecular Reproduction and Development, 90(7), 621-633.
    DOI Scopus5 WoS4 Europe PMC4
    2021 St. John, J. C. (2021). Epigenetic Regulation of the Nuclear and Mitochondrial Genomes: Involvement in Metabolism, Development, and Disease. Annual Review of Animal Biosciences, 9(1), 203-224.
    DOI Scopus1 WoS1 Europe PMC1
    2020 Kapetanovic, R., Afroz, S. F., Ramnath, D., Lawrence, G. M. E. P., Okada, T., Curson, J. E. B., . . . Sweet, M. J. (2020). Lipopolysaccharide promotes Drp1-dependent mitochondrial fission and associated inflammatory responses in macrophages. Immunology and Cell Biology, 98(7), 528-539.
    DOI Scopus42 WoS38 Europe PMC26
    2020 Towarnicki, S. G., Youngson, N. A., Corley, S. M., St John, J. C., Turner, N., Morris, M. J., & Ballard, J. W. O. (2020). Ancestral dietary change alters development of <i>Drosophila</i> larvae through MAPK signalling.
    DOI
    2019 St John, J., Makanji, Y., Johnson, J., Tsai, T. -S., Lagondar, S., Rodda, F., . . . Temple-Smith, P. (2019). The transgenerational effects of oocyte mitochondrial supplementation. Scientific Reports, 9(1), 6694-1-6694-12.
    DOI Scopus11 WoS12 Europe PMC8
    2019 St John, J. C. (2019). Genomic balance: two genomes establishing synchrony to modulate cellular fate and function. Cells, 8(11), 1-14.
    DOI Scopus8 WoS9 Europe PMC5
    2019 St John, J. C. (2019). Mitochondria and female germline stem cells−a mitochondrial DNA perspective. Cells, 8(8), 852-1-852-15.
    DOI Scopus12 WoS9 Europe PMC12
    2019 Poulton, J., Steffann, J., Burgstaller, J., McFarland, R., Arbeithuber, B., Bengoa, J., . . . Wells, D. (2019). 243rd ENMC international workshop: Developing guidelines for management of reproductive options for families with maternally inherited mtDNA disease, Amsterdam, the Netherlands, 22-24 March 2019. NEUROMUSCULAR DISORDERS, 29(9), 725-733.
    DOI WoS9 Europe PMC6
    2018 Sun, X., Vaghjiani, V., Jayasekara, W. S. N., Cain, J. E., & St John, J. C. (2018). The degree of mitochondrial DNA methylation in tumor models of glioblastoma and osteosarcoma.. Clinical epigenetics, 10(1), 157.
    DOI Scopus25 WoS26 Europe PMC21
    2018 Sun, X., & St John, J. C. (2018). Modulation of mitochondrial DNA copy number in a model of glioblastoma induces changes to DNA methylation and gene expression of the nuclear genome in tumours. Epigenetics and Chromatin, 11(1), 53-1-53-18.
    DOI Scopus27 WoS23 Europe PMC19
    2018 St John, J. C., & Tsai, T. -S. (2018). The association of mitochondrial DNA haplotypes and phenotypic traits in pigs.. BMC genetics, 19(41), 1-12.
    DOI Scopus16 WoS14 Europe PMC7
    2018 Tsai, T. -S., & St John, J. C. (2018). The effects of mitochondrial DNA supplementation at the time of fertilization on the gene expression profiles of porcine preimplantation embryos.. Molecular reproduction and development, 85(6), 490-504.
    DOI Scopus17 WoS15 Europe PMC8
    2018 Sun, X., Johnson, J., & St John, J. (2018). Global DNA methylation synergistically regulates the nuclear and mitochondrial genomes in glioblastoma cells. Nucleic Acids Research, 46(12), 5977-5995.
    DOI Scopus37 WoS37 Europe PMC31
    2018 Tsai, T., Tyagi, S., & St John, J. (2018). The molecular characterisation of mitochondrial DNA deficient oocytes using a pig model. Human Reproduction, 33(5), 942-953.
    DOI Scopus19 WoS15 Europe PMC11
    2018 Srirattana, K., & St John, J. (2018). Additional mitochondrial DNA influences the interactions between the nuclear and mitochondrial genomes in a bovine embryo model of nuclear transfer. Scientific Reports, 8(1), 17 pages.
    DOI Scopus19 WoS18 Europe PMC11
    2018 St John, J. C., Srirattana, K., Tsai, T. S., & Sun, X. (2018). The mitochondrial genome: How it drives fertility. Reproduction, Fertility and Development, 30(1), 118-139.
    DOI Scopus6 WoS5 Europe PMC3
    2017 Lee, W. T., Sun, X., Tsai, T. S., Johnson, J. L., Gould, J. A., Garama, D. J., . . . St John, J. C. (2017). Mitochondrial DNA haplotypes induce differential patterns of DNA methylation that result in differential chromosomal gene expression patterns. Cell Death Discovery, 3(1), 1-11.
    DOI Scopus29 WoS29 Europe PMC17
    2017 Srirattana, K., & St John, J. C. (2017). Manipulating the mitochondrial genome to enhance cattle embryo development. G3 : Genes, Genomes, Genetics, 7(7), 2065-2080.
    DOI Scopus17 WoS17 Europe PMC9
    2017 Vaghjiani, V., Cain, J. E., Lee, W., Vaithilingam, V., Tuch, B. E., & St John, J. C. (2017). Modulation of Mitochondrial DNA Copy Number to Induce Hepatocytic Differentiation of Human Amniotic Epithelial Cells. Stem Cells and Development, 26(20), 1505-1519.
    DOI Scopus2 WoS2 Europe PMC1
    2017 Tsai, T., Johnson, J., White, Y., & St John, J. (2017). The molecular characterization of porcine egg precursor cells. Oncotarget, 8(38), 63484-63505.
    DOI Scopus9 WoS10 Europe PMC8
    2017 Srirattana, K., McCosker, K., Schatz, T., & St John, J. C. (2017). Cattle phenotypes can disguise their maternal ancestry. BMC Genetics, 18(59), 11 pages.
    DOI Scopus19 WoS17 Europe PMC7
    2016 Sun, X., & John, J. C. S. (2016). The role of the mtDNA set point in differentiation, development and tumorigenesis. Biochemical Journal, 473(19), 2955-2971.
    DOI Scopus35 WoS30 Europe PMC17
    2016 Tsai, T., & St John, J. C. (2016). The role of mitochondrial DNA copy number, variants, and haplotypes in farm animal developmental outcome. Domestic Animal Endocrinology, 56, S133-S146.
    DOI Scopus12 WoS12 Europe PMC5
    2016 Lee, W. T. Y., & St. John, J. C. (2016). Mitochondrial DNA as an initiator of tumorigenesis. Cell Death and Disease, 7(3), 2 pages.
    DOI Scopus9 WoS8 Europe PMC6
    2016 Cagnone, G., Tsai, T. S., Srirattana, K., Rossello, F., Powell, D. R., Rohrer, G., . . . St John, J. C. (2016). Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics, 202(3), 931-944.
    DOI Scopus20 WoS20 Europe PMC15
    2016 Tsai, T., Rajasekar, S., & St John, J. (2016). The relationship between mitochondrial DNA haplotype and the reproductive capacity of domestic pigs (Sus scrofa domesticus). BMC Genetics, 17(1), 67.
    DOI Scopus34 WoS32 Europe PMC17
    2016 St John, J. C., Tsai, T. S., & Cagnone, G. L. M. (2016). Mitochondrial DNA supplementation as an enhancer of female reproductive capacity. Current Opinion in Obstetrics and Gynecology, 28(3), 211-216.
    DOI Scopus4 WoS4 Europe PMC3
    2016 Cagnone, G. L. M., Tsai, T. S., Makanji, Y., Matthews, P., Gould, J., Bonkowski, M. S., . . . John, J. C. S. (2016). Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency. Scientific Reports, 6(1), 1-15.
    DOI Scopus66 WoS55 Europe PMC38
    2016 St John, J. C. (2016). Mitochondrial DNA copy number and replication in reprogramming and differentiation. Seminars in Cell and Developmental Biology, 52, 93-101.
    DOI Scopus44 WoS35 Europe PMC26
    2016 Johnson, J., Lee, W., Frazier, A. E., Vaghjiani, V., Laskowski, A., Rodriguez, A. L., . . . St John, J. C. (2016). Deletion of the Complex I Subunit NDUFS4 Adversely Modulates Cellular Differentiation. Stem Cells and Development, 25(3), 239-250.
    DOI Scopus7 WoS7 Europe PMC4
    2016 Lee, W. T. Y., Cain, J. E., Cuddihy, A., Johnson, J., Dickinson, A., Yeung, K. Y., . . . St John, J. C. (2016). Mitochondrial DNA plasticity is an essential inducer of tumorigenesis. Cell Death Discovery, 2(1), 11 pages.
    DOI Scopus26 WoS26 Europe PMC19
    2015 Lee, W., Johnson, J., Gough, D. J., Donoghue, J., Cagnone, G. L. M., Vaghjiani, V., . . . St John, J. C. (2015). Mitochondrial DNA copy number is regulated by DNA methylation and demethylation of POLGA in stem and cancer cells and their differentiated progeny. Cell Death and Disease, 6(2), e1664.
    DOI Scopus78 WoS74 Europe PMC54
    2015 Lee, W. T. Y., & John, J. S. (2015). The control of mitochondrial DNA replication during development and tumorigenesis. Annals of the New York Academy of Sciences, 1350(1), 95-106.
    DOI Scopus39 WoS34 Europe PMC21
    2015 Henry, B. A., Loughnan, R., Hickford, J., Young, I. R., St John, J. C., & Clarke, I. (2015). Differences in mitochondrial DNA inheritance and function align with body conformation in genetically lean and fat sheep. Journal of Animal Science, 93(5), 2083-2093.
    DOI Scopus10 WoS10 Europe PMC7
    2015 Wu, L., Russell, D., Wong, S., Chen, M., Tsai, T., St John, J., . . . Robker, R. (2015). Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors. Development, 142(4), 681-691.
    DOI Scopus197 WoS184 Europe PMC125
    2015 St John, J. C. (2015). The mitochondrion, its genome and their contribution to well-being and disease. Molecular Human Reproduction, 21(1), 1-2.
    DOI Scopus5 WoS2 Europe PMC4
    2014 St John, J., Srirattana, K., & Clarke, I. (2014). Modifying the mitochondrial genome to enhance animal production. Australasian Biotechnology, 24(3), 61.
    2014 Yeung, K. Y., Dickinson, A., Donoghue, J. F., Polekhina, G., White, S. J., Grammatopoulos, D. K., . . . St John, J. C. (2014). The identification of mitochondrial DNA variants in glioblastoma multiforme. Acta Neuropathologica Communications, 2(1), 1.
    DOI Scopus44 WoS45 Europe PMC51
    2014 Sobinoff, A. P., Sutherland, J. M., Beckett, E. L., Stanger, S. J., Johnson, R., Jarnicki, A. G., . . . Mclaughlin, E. A. (2014). Damaging legacy: Maternal cigarette smoking has long-term consequences for male offspring fertility. Human Reproduction, 29(12), 2719-2735.
    DOI Scopus43 WoS41 Europe PMC25
    2014 St John, J. (2014). The control of mtDNA replication during differentiation and development. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1345-1354.
    DOI Scopus78 WoS73 Europe PMC52
    2014 Nagley, P., St John, J., Gabriel, K., & McKenzie, M. (2014). Mitochondrial research in Australia: A major player in worldwide trends. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1225-1226.
    DOI
    2014 Mckenzie, M., Chiotis, M., Hroudová, J., Lopez Sanchez, M., Lim, S., Cook, M., . . . Trounce, I. (2014). Capture of somatic mtDNA point mutations with severe effects on oxidative phosphorylation in synaptosome cybrid clones from human brain. Human Mutation, 35(12), 1476-1484.
    DOI Scopus12 WoS12 Europe PMC8
    2014 Lee, T., Clarke, I., St John, J., Young, I., Leury, B., Rao, A., . . . Henry, B. (2014). High cortisol responses identify propensity for obesity that is linked to thermogenesis in skeletal muscle. FASEB Journal, 28(1), 35-44.
    DOI Scopus15 WoS15 Europe PMC9
    2013 Dickinson, A., Yeung, K. Y., Donoghue, J., Baker, M. J., Kelly, R. D., McKenzie, M., . . . St John, J. C. (2013). The regulation of mitochondrial DNA copy number in glioblastoma cells. Cell Death and Differentiation, 20(12), 1644-1653.
    DOI Scopus98 WoS97 Europe PMC76
    2013 Kelly, R. D. W., Rodda, A. E., Dickinson, A., Mahmud, A., Nefzger, C. M., Lee, W., . . . St John, J. C. (2013). Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells. Stem Cells, 31(4), 703-716.
    DOI Scopus62 WoS63 Europe PMC43
    2013 Kelly, R. D. W., Sumer, H., McKenzie, M., Facucho-Oliveira, J., Trounce, I. A., Verma, P. J., & St John, J. C. (2013). The Effects of Nuclear Reprogramming on Mitochondrial DNA Replication. Stem Cell Reviews and Reports, 9(1), 1-15.
    DOI Scopus44 WoS39 Europe PMC28
    2013 John, J. S. (2013). A tribute to somatic cell reprogrammers. Molecular Human Reproduction, 19(2), 55-56.
    DOI
    2012 Kelly, R. D. W., Mahmud, A., McKenzie, M., Trounce, I. A., & St John, J. C. (2012). Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A. Nucleic Acids Research, 40(20), 10124-10138.
    DOI Scopus143 WoS129 Europe PMC106
    2012 St John, J. C. (2012). Transmission, inheritance and replication of mitochondrial DNA in mammals: Implications for reproductive processes and infertility. Cell and Tissue Research, 349(3), 795-808.
    DOI Scopus23 WoS22 Europe PMC9
    2011 Rae, P. C., Kelly, R. D., Egginton, S., & St John, J. C. (2011). Angiogenic potential of endothelial progenitor cells and embryonic stem cells. Vascular Cell, 3(1), 15 pages.
    DOI Scopus33 WoS30 Europe PMC19
    2011 Sanchez-Partida, L., Kelly, R., Sumer, H., Lo, C., Aharon, R., Holland, M., . . . John St, J. (2011). The generation of live offspring from vitrified oocytes. PLoS ONE, 6(6), e21597.
    DOI Scopus18 WoS14 Europe PMC10
    2011 Jiang, Y., Kelly, R., Peters, A., Fulka, H., Dickinson, A., Mitchell, D. A., & St. John, J. C. (2011). Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors. PLoS ONE, 6(4), 12 pages.
    DOI Scopus43 WoS27 Europe PMC19
    2011 Kelly, R. D. W., & St. John, J. C. (2011). Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells. International Journal of Developmental Biology, 54(11-12), 1659-1670.
    DOI Scopus16 WoS11 Europe PMC11
    2010 St John, J. C., & Campbell, K. H. S. (2010). The battle to prevent the transmission of mitochondrial DNA disease: Is karyoplast transfer the answer. Gene Therapy, 17(2), 147-149.
    DOI Scopus16 WoS15 Europe PMC8
    2010 St. John, J. C., Facucho-Oliveira, J., Jiang, Y., Kelly, R., & Salah, R. (2010). Mitochondrial DNA transmission, replication and inheritance: A journey from the gamete through the embryo and into offspring and embryonic stem cells. Human Reproduction Update, 16(5), 488-509.
    DOI Scopus211 WoS191 Europe PMC137
    2010 Lee, J. H., Peters, A., Fisher, P., Bowles, E. J., St. John, J. C., & Campbell, K. H. S. (2010). Generation of mtDNA homoplasmic cloned lambs. Cellular Reprogramming, 12(3), 347-355.
    DOI Scopus28 WoS21 Europe PMC14
    2009 Fulka, J., Loi, P., Ptak, G., Fulka, H., & John, J. S. (2009). Hope for the mammoth?. Cloning and Stem Cells, 11(1), 1-3.
    DOI Scopus7 WoS7 Europe PMC9
    2009 Facucho-Oliveira, J. M., & John St., J. C. (2009). The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic Stem Cell differentiation. Stem Cell Reviews and Reports, 5(2), 140-158.
    DOI Scopus199 WoS179 Europe PMC138
    2009 Taylor, R. A., Wang, H., Wilkinson, S. E., Richards, M. G., Britt, K. L., Vaillant, F., . . . Risbridger, G. P. (2009). Lineage enforcement by inductive mesenchyme on adult epithelial stem cells across developmental germ layers. Stem Cells, 27(12), 3032-3042.
    DOI Scopus25 WoS26 Europe PMC19
    2008 Fulka, J., Fulka, H., St John, J., Galli, C., Lazzari, G., Lagutina, I., . . . Loi, P. (2008). Cybrid human embryos - warranting opportunities to augment embryonic stem cell research. Trends in Biotechnology, 26(9), 469-474.
    DOI Scopus5 WoS5 Europe PMC3
    2008 Bowles, E. J., Tecirlioglu, R. T., French, A. J., Holland, M. K., & St. John, J. C. (2008). Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells, 26(3), 775-782.
    DOI Scopus40 WoS34 Europe PMC23
    2008 St John, J. C., Armstrong, L., Minger, S. L., & Campbell, K. H. S. (2008). Law should recognize value of interspecies embryos [1]. Nature, 451(7179), 627.
    DOI Scopus5 WoS6 Europe PMC3
    2007 St John, J., & Lovell-Badge, R. (2007). Human-animal cytoplasmic hybrid embryos, mitochondria, and an energetic debate. Nature Cell Biology, 9(9), 988-992.
    DOI Scopus26 WoS19 Europe PMC15
    2007 Bowles, E. J., Lee, J. H., Alberio, R., Lloyd, R. E. I., Stekel, D., Campbell, K. H. S., & St. John, J. C. (2007). Contrasting effects of in vitro fertilization and nuclear transfer on the expression of mtDNA replication factors. Genetics, 176(3), 1511-1526.
    DOI Scopus50 WoS48 Europe PMC36
    2007 St John, J. C., Bowles, E. J., & Amaral, A. (2007). Sperm mitochondria and fertilisation.. Society of Reproduction and Fertility supplement, 65, 399-416.
    Scopus21 Europe PMC11
    2007 Spikings, E. C., Alderson, J., & St. John, J. C. (2007). Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. Biology of Reproduction, 76(2), 327-335.
    DOI Scopus210 WoS194 Europe PMC142
    2007 Amaral, A., Ramalho-Santos, J., & St John, J. C. (2007). The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Human Reproduction, 22(6), 1585-1596.
    DOI Scopus110 WoS100 Europe PMC66
    2007 Facucho-Oliveira, J. M., Alderson, J., Spikings, E. C., Egginton, S., & St. John, J. C. (2007). Mitochondrial DNA replication during differentiation of murine embryonic stem cells. Journal of Cell Science, 120(22), 4025-4034.
    DOI Scopus235 WoS228 Europe PMC183
    2007 Fulka, J., Fulka, H., & St. John, J. C. (2007). Transmission of mitochondrial DNA disorders: Possibilities for the elimination of mutated mitochondria. Cloning and Stem Cells, 9(1), 47-50.
    DOI Scopus8 WoS6 Europe PMC5
    2006 Spikings, E. C., Alderson, J., & St. John, J. C. (2006). Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer. Human Reproduction Update, 12(4), 401-415.
    DOI Scopus63 WoS60 Europe PMC34
    2006 Poulton, J., Kennedy, S., Oakeshott, P., & St John, J. (2006). Nuclear transfer to prevent mitochondrial DNA diseases. Lancet, 368(9538), 841.
    DOI Scopus9 WoS7 Europe PMC2
    2006 Santos, T. A., El Shourbagy, S., & St. John, J. C. (2006). Mitochondrial content reflects oocyte variability and fertilization outcome. Fertility and Sterility, 85(3), 584-591.
    DOI Scopus312 WoS292 Europe PMC203
    2006 Lloyd, R. E., Lee, J. H., Alberio, R., Bowles, E. J., Ramalho-Santos, J., Campbell, K. H. S., & St. John, J. C. (2006). Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. Genetics, 172(4), 2515-2527.
    DOI Scopus59 WoS54 Europe PMC38
    2006 St John, J. C., Amaral, A., Bowles, E., Oliveira, J. F., Lloyd, R., Freitas, M., . . . Ramalho-Santos, J. (2006). The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells.. Methods in molecular biology (Clifton, N.J.), 331, 347-374.
    DOI Scopus46 Europe PMC38
    2006 El Shourbagy, S. H., Spikings, E. C., Freitas, M., & St. John, J. C. (2006). Mitochondria directly influence fertilisation outcome in the pig. Reproduction, 131(2), 233-245.
    DOI Scopus265 WoS246 Europe PMC181
    2005 Fleming, C., Maldjian, A., Da Costa, D., Rullay, A. K., Haddleton, D. M., St John, J., . . . Davis, B. G. (2005). A Carbohydrate-Antioxidant Hybrid Polymer Reduces Oxidative Damage in Spermatozoa and Enhances Fertility. Nature Chemical Biology, 1(5), 270-274.
    DOI Scopus64 WoS59 Europe PMC18
    2005 St. John, J. C., Moffatt, O., & D'Souza, N. (2005). Aberrant heteroplasmic transmission of mtDNA in cloned pigs arising from double nuclear transfer. Molecular Reproduction and Development, 72(4), 450-460.
    DOI Scopus35 WoS30 Europe PMC22
    2005 St John, J. C., & Alderson, J. (2005). Stem-cell banking: The size of the task. Lancet, 366(9502), 1991-1992.
    DOI Scopus3 WoS1 Europe PMC2
    2005 St. John, J. C., Ramalho-Santos, J., Gray, H. L., Petrosko, P., Rawe, V. Y., Navara, C. S., . . . Schatten, G. P. (2005). The expression of mitochondrial DNA transcription factors during early cardiomyocyte in vitro differentiation from human embryonic stemn cells. Cloning and Stem Cells, 7(3), 141-153.
    DOI Scopus209 WoS185 Europe PMC154
    2005 St. John, J. C., Jokhi, R. P., & Barratt, C. L. R. (2005). The impact of mitochondrial genetics on male infertility. International Journal of Andrology, 28(2), 65-73.
    DOI Scopus96 WoS74 Europe PMC60
    2004 St. John, J. C., & Schatten, G. (2004). Paternal mitochondrial DNA transmission during nonhuman primate nuclear transfer. Genetics, 167(2), 897-905.
    DOI Scopus65 WoS51 Europe PMC32
    2004 St. John, J. C., Lloyd, R. E. I., Bowles, E. J., Thomas, E. C., & El Shourbagy, S. (2004). The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective. Reproduction, 127(6), 631-641.
    DOI Scopus72 WoS59 Europe PMC40
    2004 Mital, D., White, D. J., & St John, J. C. (2004). Mitochondrial DNA and sperm quality in patients on antiretroviral therapy - Response [3]. AIDS, 18(6), 963.
    DOI Scopus4 WoS4 Europe PMC2
    2004 Barratt, C. L. R., St. John, J. C., & Afnan, M. (2004). Clinical challenges in providing embryos for stem-cell initiatives. Lancet, 364(9429), 115-118.
    DOI Scopus21 WoS17 Europe PMC12
    2004 St John, J. C., Lloyd, R., & El Shourbagy, S. (2004). The potential risks of abnormal transmission of mtDNA through assisted reproductive technologies. Reproductive BioMedicine Online, 8(1), 34-44.
    DOI Scopus30 WoS23 Europe PMC11
    2003 Payne, C., St. John, J. C., Ramalho-Santos, J., & Schatten, G. (2003). LIS1 Association with Dynactin is Required for Nuclear Motility and Genomic Union in the Fertilized Mammalian Oocyte. Cell Motility and the Cytoskeleton, 56(4), 245-251.
    DOI Scopus17 WoS16 Europe PMC12
    2002 Perkins, J., St. John, J., & Ahmed, A. (2002). Modulation of trophoblast cell death by oxygen and EGF. Molecular Medicine, 8(12), 847-856.
    DOI Scopus25 WoS24 Europe PMC21
    2002 St. John, J. C. (2002). Ooplasm donation in humans: The need to investigate the transmission of mitochondrial DNA following cytoplasmic transfer. Human Reproduction, 17(8), 1954-1958.
    DOI Scopus46 WoS42 Europe PMC26
    2001 White, D. J., Mital, D., Taylor, S., & John, J. C. S. (2001). Sperm mitochondrial DNA deletions as a consequence of long term highly active antiretroviral therapy. AIDS, 15(8), 1061-1062.
    DOI Scopus55 WoS39 Europe PMC24
    2001 St. John, J. C., Jokhi, R. P., & Barratt, C. L. R. (2001). Men with oligoasthenoteratozoospermia harbour higher numbers of multiple mitochondrial DNA deletions in their spermatozoa, but individual deletions are not indicative of overall aetiology. Molecular Human Reproduction, 7(1), 103-111.
    DOI Scopus88 WoS74 Europe PMC52
    2000 John, J. C. S., & De Jonge, C. J. (2000). A hypothesis for transmission of paternal mitochondrial DNA. Reproductive Medicine Review, 8(1), 73-85.
    DOI Scopus6
    2000 St. John, J. C., Sakkas, D., & Barratt, C. L. R. (2000). A role for mitochondrial DNA and sperm survival. Journal of Andrology, 21(2), 189-199.
    Scopus55 WoS57 Europe PMC32
    2000 Ahmed, A., Rahman, M., Zhang, X., Acevedo, C. H., Nijjar, S., Rushton, I., . . . St John, J. (2000). Induction of placental heme oxygenase-1 is protective against TNFalpha-induced cytotoxicity and promotes vessel relaxation.. Molecular medicine (Cambridge, Mass.), 6(5), 391-409.
    DOI Scopus140 WoS132 Europe PMC99
    2000 St John, J., Sakkas, D., Dimitriadi, K., Barnes, A., Maclin, V., Ramey, J., . . . De Jonge, C. (2000). Failure of elimination of paternal mitochondrial DNA in abnormal embryos. Lancet, 355(9199), 200.
    DOI Scopus80 WoS71 Europe PMC42
    1999 St. John, J. C. (1999). Incorporating molecular screening techniques into the modern andrology laboratory. Journal of Andrology, 20(6), 692-701.
    Scopus9 WoS4 Europe PMC1
    1999 Sakkas, D., Mariethoz, E., & St. John, J. C. (1999). Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the fas-mediated pathway. Experimental Cell Research, 251(2), 350-355.
    DOI Scopus301 WoS251 Europe PMC160
    1997 St. John, J. C., Cooke, I. D., Barratt, C. L. R., & Johns, D. R. (1997). Mitochondrial mutations and male infertility [2]. Nature Medicine, 3(2), 124-125.
    DOI Scopus59 WoS68 Europe PMC29
    1997 St John, J. C., Barratt, C. L. R., Brenner, C. A., Scott, J., & Cohen, J. (1997). Use of anucleate donor oocyte cytoplasm in recipient eggs (multiple letters) [16]. Lancet, 350(9082), 961-962.
    DOI Scopus2 WoS12 Europe PMC9
    - St. John, J. C. (n.d.). Mitochondrial
    <scp>DNA</scp>
    : Fate of the Paternal Mitochondrial Genome.

    DOI
  • Books

    Year Citation
    2013 St. John, J. C. (2013). Mitochondrial DNA, mitochondria, disease and stem cells. J. C. St. John (Ed.), Humana Press.
    DOI
    2007 St John, J. C. (2007). Preface (Vol. 77). Elsevier.
    DOI
    2007 Bowles, E. J., Campbell, K. H. S., & St. John, J. C. (2007). Nuclear Transfer: Preservation of a Nuclear Genome at the Expense of Its Associated mtDNA Genome(s) (Vol. 77). J. C. StJohn (Ed.), ELSEVIER ACADEMIC PRESS INC.
    DOI Scopus45 WoS36 Europe PMC23
  • Book Chapters

    Year Citation
    2019 Srirattana, K., & St John, J. (2019). Transmission of Dysfunctional Mitochondrial DNA and Its Implications for Mammalian Reproduction. In P. Sutovsky (Ed.), Cellular and Molecular Basis of Mitochondrial Inheritance (Vol. 231, pp. 75-103). Switzerland: Springer Nature.
    DOI Scopus12 WoS11 Europe PMC5
    2016 Cagnone, G., Vaghjiani, V., Lee, W., Sun, C., Johnson, J., Yeung, K. Y., & St John, J. C. (2016). Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. In K. Turksen, & A. Nagy (Eds.), Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Vol. 1357, pp. 231-267). New York; USA: Springer.
    DOI Scopus2 Europe PMC2
    2016 Sun, X., Lee, W., Vaghjiani, V., & St John, J. C. (2016). Analysis of mitochondrial DNA copy number and its regulation through DNA methylation of POLGA. In M. McKenzie (Ed.), Mitochondrial DNA: Methods and Protocols (Vol. 1351, pp. 131-141). New York; USA: Springer.
    DOI Scopus5 Europe PMC3
    2015 Lee, W., Kelly, R. D. W., Yeung, K. Y., Cagnone, G., McKenzie, M., & St John, J. C. (2015). Analysis of mitochondrial DNA in induced pluripotent and embryonic stem cells. In P. J. Verma, & H. Sumer (Eds.), Cell Reprogramming: Methods and Protocols (Vol. 1330, pp. 219-252). New York; USA: Springer.
    DOI Scopus3 Europe PMC1
    2013 Yeung, K. Y., Dickinson, A., & St. John, J. C. (2013). The Role of Mitochondrial DNA in Tumorigenesis. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 119-155). Humana Press.
    DOI Scopus1
    2013 Facucho-Oliveira, J., Kulkarni, T., Machado-Oliveira, G., & St. John, J. C. (2013). From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 87-118). Humana Press.
    DOI
    2013 St. John, J. C. (2013). Mitochondrial DNA: Its Transmission from Gametes and Embryos. In Principles of Cloning: Second Edition (pp. 429-439). Elsevier.
    DOI Scopus1
    2013 Kelly, R. D. W., Mahmud, A., & St. John, J. C. (2013). Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 157-183). Humana Press.
    DOI
    2012 St. John, J. C., & Campbell, K. H. S. (2012). The consequences of reprogramming a somatic cell for mitochondrial DNA transmission, inheritance and replication. In Nuclear Reprogramming and Stem Cells (pp. 83-97). Humana Press.
    DOI
  • Conference Papers

    Year Citation
    2014 St John, J. C. (2014). SUPPLEMENTING OOCYTES WITH AUTOLOGOUS MITOCHONDRIA ENHANCES FERTILIZATION OUTCOMES.. In FERTILITY AND STERILITY Vol. 102 (pp. E330). Honolulu, HI: ELSEVIER SCIENCE INC.
    DOI
    2009 St. John, J. C. (2009). mtDNA and sperm function. In Systems Biology in Reproductive Medicine Vol. 55 (pp. 17-18).
    2008 Fulka, H., St. John, J. C., Fulka, J., & Hozák, P. (2008). Chromatin in early mammalian embryos: Achieving the pluripotent state. In Differentiation Vol. 76 (pp. 3-14). England: ELSEVIER SCI LTD.
    DOI Scopus50 WoS43 Europe PMC29
    2004 Ramalho-Santos, J., Sammak, P., Chong, K. Y., Qidwai, H., Rawe, V. Y., St John, J. C., & Schatten, G. (2004). Membrane trafficking and organelle dynamics in undifferentiated embryonic stem cells: Endomembrane biogenesis and expansion coupled with differentiation.. In BIOLOGY OF REPRODUCTION (pp. 216-217). Univ British Columbia, Vancouver, CANADA: SOC STUDY REPRODUCTION.
    2002 St. John, J. C. (2002). The transmission of mitochondrial DNA following assisted reproductive techniques. In Theriogenology Vol. 57 (pp. 109-123). FOZ DO IGUACU, BRAZIL: ELSEVIER SCIENCE INC.
    DOI Scopus42 WoS39 Europe PMC28
    2000 O'Connell, M., St John, J. C., McClure, N., Steele, E. K., & Lewis, S. E. M. (2000). A comparison of mitochondrial DNA deletions in proximal epididymal and testicular spermatozoa in males with obstructive azoospermia. In HUMAN REPRODUCTION Vol. 15 (pp. 114-115). OXFORD UNIV PRESS.
    1999 Barratt, C. L. R., St John, J. C., & Afnan, M. (1999). Genetic testing of the male.. In C. Gagnon (Ed.), MALE GAMETE: FROM BASIC SCIENCE TO CLINICAL APPLICATIONS (pp. 397-405). MONTREAL, CANADA: CACHE RIVER PRESS.
    WoS1
    1998 Barratt, C. L. R., & St John, J. C. (1998). Diagnostic tools in male infertility. In Human Reproduction Vol. 13 (pp. 51-61). THESSALONIKI, GREECE: OXFORD UNIV PRESS.
    DOI Scopus13 WoS9 Europe PMC3
    1997 St John, J. C., Cooke, I. D., & Barratt, C. L. R. (1997). The Use of Long PCR to detect multiple deletions in the mitochondrial DNA of human testicular tissue from azoospermic and severe oligozoospermic patients. In C. Barratt, C. DeJonge, D. Mortimer, & J. Parinaud (Eds.), GENETICS OF HUMAN MALE FERTILITY (pp. 333-347). COLLIOURE, FRANCE: EDITIONS EDK.
    WoS6
    1997 StJohn, J. C., Jokhi, R. P., Barratt, C. L. R., & Cooke, I. D. (1997). The effects of mitochondrial dysfunction on sperm motility. In HUMAN REPRODUCTION Vol. 12 (pp. P11). OXFORD UNIV PRESS.
  • Conference Items

    Year Citation
    2014 Chang, J. R., Chang, C. W., Chao, S. J., Cheng, A., Dai, K., Lee, J. T., . . . Yang, C. P. (2014). Preface. Poster session presented at the meeting of Geotechnical Special Publication.

2021 – 2025    NHMRC Ideas Grant (GNT2000723), Understanding the benefits and limitations of metaphase II spindle transfer (St. John J) $1,629,373.

2019 – 2022: NHMRC Project Grant (GNT1160106), Understanding how mitochondrial DNA contributes to embryo development (St. John J) $669,790

2018 – 2020: NHMRC Development Grant (GNT1136065), Improving oocyte mitochondrial DNA copy number to enhance female reproductive capacity (St. John J, McKenzie W, Western P, Rombauts L) $670,867.20.

2017 – 2018: The Winston Foundation (WF1), Developing a canine in vitro maturation culture system (St. John J) $42,000

2016 – 2018: OvaScience Inc. Waltham, USA. Renewal Grant, Safety of Mitochondrial Transfer (St. John J) $600,000.

2016 – 2019: ARC Discovery Grant(DP160102575), Diet influences the selective advantage of mitochondrial DNA mutations (Ballard B, St. John J, Smyth G) $313,600.00

2015 – 2017: NHMRC Project Grant(GNT1079309) - Metabolic and molecular basis of embryo signalling (Lane M, St. John J, Zander-Fox D) $397,077.00

2014 – 2016: Australian Mitochondrial Disease Foundation- Preventing the transmission of mutant mitochondrial DNA (St. John J) $25,000

2013 – 2016: OvaScience Inc- Determining the safety of homologous mitochondrial transfer in a mini-pig model (St. John J) $1,250,000

2013 – 2015: NHMRC Project Grant(GNT1041471) - Understanding mitochondrial DNA segregation and transmission (CIA - St. John J; Trounce I) $492,863

2013 – 2015: Zwart Estate- The role of mtDNA variants in glioblastoma (St. John J) $133,131

2013 – 2016: Australian Pork Limited- Defining the role of mitochondrial DNA in pig fertility (St. John J) $92,637

2013 – 2014: Johnston Estate- The generation of stem cell models of mtDNA disease (St. John J) $170,000

2012 – 2014: NHMRC Project Grant(GNT1022222) - Understanding the pathogenesis of mitochondrial disease using iPS cells (Thorburn D, St. John J, Frazier A, Nisbet D, White S) $618,675

2011: Marian and EH Flack Trust- Funding to purchase a High Resolution Melting Curve Analysis Machine(White S, Western P, St. John J, Watkins DN) $30,000.

  • Committee Memberships

    Date Role Committee Institution Country
    2019 - 2020 Member Mitochondrial Donation Expert Working Committee National Health and Medical Research Council Australia
    2018 - 2021 Member Embryo Research Licencing Committee National Health and Medical Reseach Council Australia
  • Position: NHMRC Grant-Funded Researchr E
  • Email: jus.stjohn@adelaide.edu.au
  • Campus: North Terrace
  • Building: Adelaide Health and Medical Sciences, floor 5
  • Org Unit: Medical Sciences

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