Adelaide Medical School
Faculty of Health and Medical Sciences
Eligible to supervise Masters and PhD - email supervisor to discuss availability.
The overall aim of my research program is to understand how the nuclear and mitochondrial genomes interact during development in order that cells, tissues and organs function efficiently. We undertake these studies using oocytes, preimplantation embryos, embryonic and adult stem cells, somatic cells, and tumour-initiating cells. We use a number of oocyte, embryo and cell manipulation techniques, next generation sequencing technologies and bioinformatics. Our work is divided into three areas, which we will continue to interrogate over the next five years.
Research Focus One: Understanding the role of mitochondrial DNA (mtDNA) in oocyte quality and development outcomes.
We undertake this area of research using porcine and bovine oocytes and generate embryos and offspring through in vitrofertilisation (IVF), nuclear transfer (NT) cytoplasmic transfer (CT), and mitochondrial supplementation (mICSI). This enables us to determine how the nuclear and mtDNA content of an oocyte affects fertilisation and developmental outcome; and how mtDNA segregation, transmission and replication are normally regulated, as is the case following IVF, and perturbed as these mechanisms are violated by NT and CT.
We are currently funded by the NHMRC (Development Grant) to generate mini-pig models using a new assisted reproductive technology that we have been developing, which involves supplementing oocytes deficient in mtDNA with genetically identical populations of mtDNA. This technology is known as mICSI,and our published data suggest that mICSI enhances embryo quality and overcomes the predisposition of poor quality oocytes to give rise to diabetes and obesity. The grant will determine whether supplementing eggs at the time of fertilisation with genetically identical mtDNA is safe practice. Furthermore, we are also funded through an NHMRC Project Grant to determine the mechanisms associated with mICSI.
We have also previously generated a derivation of another assisted reproductive technology, namely cloning or somatic cell nuclear transfer (SCNT), that ensures cloned embryos inherit their mtDNA from the population present in the oocyte only, as is the case following natural conception, and not from the somatic cell as well. This technology, known as mito-SCNT, improves embryo quality and developmental outcomes and overcomes the problem of embryos and offspring inheriting two populations of mtDNA.
Research Focus Two: Defining the role of the mtDNA set point in early development and cancer.
In recent years, we have discovered the ‘mtDNA set point’, which is a key developmental milestone that naïve (pluripotent) cells must acquire in order to initiate and complete their differentiation into mature cell types. The mtDNA set point is characterised by cells expressing pluripotent markers and having very low mtDNA copy number, which are both regulated by the epigenetic status of the nuclear genome. Furthermore, we have shown that key nuclear-encoded mtDNA replication factors are DNA methylated in a tissue specific manner that accounts for cell-specific mtDNA copy number, which requires the establishment of the mtDNA set point early during development. Our studies have also shown that failure to establish the mtDNA set point results in developmental failure and is a key characteristic of tumour-initiating cells. In addition, by depleting tumour-initiating cells of their mtDNA, we have shown that the incidence of tumour formation is significantly reduced (glioblastoma mutiforme and osteosarcoma) or prevented (multiple myeloma), as these cells re-establish the mtDNA set point and undergo differentiation and, therefore, do not form tumours.
We use and derive embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and tumour-initiating cell lines and tumours to perform these studies. These models allow us to focus on molecular mechanisms and to identify the specific windows during differentiation/development when these events manifest and then to confirm and characterise these events through, for example, targeted gene knockdown and overexpression.
Research Focus Three: Determining how mtDNA haplotypes influence phenotype.
Recent outcomes from our research program have shown that mtDNA halpotypes influence livestock phenotypes, such as meat quality and lifetime daily gains in pigs, and reproductive efficiencies in pigs and cattle. Using stem cells models, we have been able to demonstrate that mtDNA haplotypes induce phenotypic changes through modulation of DNA methylation patterns that, in turn, affect chromosomal gene expression patterns. Using tumour cells, we have also demonstrated differences in the onset and progression of tumours based on mtDNA haplotype alone.
CURRENT RESEARCH PROJECTS AVAILABLE FOR STUDENTS:
RESEARCH PROJECT 1: Identifying variants in the mitochondrial genome predictive of oocyte quality.
Project description: A number of mitochondrial DNA variants have been identified in oocytes. However, their affect and role have yet to be properly defined, especially in oocytes from offspring derived from assisted reproductive technologies that perturb the maternal only transmission of mitochondrial DNA. Using next generation sequencing and bioinformatics tools, this project will focus on identifying variants in the mitochondrial genome that are predictive of oocyte quality and whether an oocyte will progress to fertilise and develop into an embryo. Using an experimental model of pig oocytein vitromaturation, you will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitrofertilisation protocols. After preparing fertilised and failed to fertilise oocyte samples for next generation mitochondrial DNA sequencing technology, you will learn to apply bioinformatics tools to identify and quantify variants that are associated with good and poor oocyte quality. These outputs will then be tested in: i) a larger cohort of oocytes from commercial breeding populations and matched to data associated with economic breeding values; and ii) oocytes derived from offspring that were generated from assisted reproductive technologies that result in two populations of mitochondrial DNA being transmitted.
Projects available for:Honours / HDR / Masters / Mphil / PhD
Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Level 5.
Research project start: Semester 1 and 2
Special requirements: Vaccination required.
RESEARCH PROJECT 2: Identifying global DNA methylation patterns associated in the preimplantation embryo.
Project description: With the introduction of more invasive assisted reproductive technologies into animal breeding programs and their potential implementation into human clinical IVF, we need to understand how and when they modify the epigenome during early development, especially technologies that perturb the balance between the nuclear and mitochondrial genomes. DNA methylation is a key epigenetic mechanism that regulates gene expression during early development. Using a porcine oocyte and embryo in vitromodel, next generation bisulfite DNA sequencing and bioinformatics tools, this project will focus on mapping the DNA methylation profiles of oocytes and early embryos to define the waves of DNA methylation prior to and post-embryonic genome activation, the time point when the early embryo initiates its own global transcription. You will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitrofertilisation protocols. After preparing fertilised oocyte and embryo samples for next generation bisulfite DNA sequencing, you will learn to apply bioinformatics tools to map regions of DNA methylation across the whole genome and validate the findings. These outputs will then be tested against embryos generated from other assisted reproductive technologies to determine how these technologies impact on the DNA methylation profiles of the developing embryo.
Projects available for: Third Year / Honours / HDR / Masters / Mphil / PhD
Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Level 5.
Research project start: Semester 1 and 2
Special requirements: Vaccination required.
Date Position Institution name 2019 Professor University of Adelaide 2009 - 2019 Professor and Centre Head Hudson Institute of Medical Research 2007 - 2009 Professor of Reproductive Biology University of Warwick 2000 - 2007 Lecturer University of Birmingham
Language Competency German Can read, write, speak, understand spoken and peer review
Date Institution name Country Title 1999 University of Birmingham United Kingdom PhD 1995 University of Sheffield United Kingdom BSc (Hons)
Year Citation 2019 St John, J. C. (2019). Mitochondria and female germline stem cells−a mitochondrial DNA perspective. Cells, 8(8), 852-1-852-15.
2019 Poulton, J., Steffann, J., Burgstaller, J., McFarland, R., Arbeithuber, B., Bengoa, J., . . . Wells, D. (2019). 243rd ENMC international workshop: Developing guidelines for management of reproductive options for families with maternally inherited mtDNA disease, Amsterdam, the Netherlands, 22-24 March 2019. NEUROMUSCULAR DISORDERS, 29(9), 725-733.
2019 St John, J., Makanji, Y., Johnson, J., Tsai, T., Lagondar, S., Rodda, F., . . . Temple-Smith, P. (2019). The transgenerational effects of oocyte mitochondrial supplementation. Scientific Reports, 9(1), 12 pages.
DOI Scopus2 WoS3 Europe PMC1
2018 Sun, X., Vaghjiani, V., Jayasekara, W. S. N., Cain, J. E., & St John, J. C. (2018). The degree of mitochondrial DNA methylation in tumor models of glioblastoma and osteosarcoma.. Clinical epigenetics, 10(1), 157.
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2018 Sun, X., & St John, J. C. (2018). Modulation of mitochondrial DNA copy number in a model of glioblastoma induces changes to DNA methylation and gene expression of the nuclear genome in tumours. Epigenetics & chromatin, 11(1), 53.
DOI Scopus2 WoS2 Europe PMC1
2018 St John, J. C., & Tsai, T. -S. (2018). The association of mitochondrial DNA haplotypes and phenotypic traits in pigs.. BMC genetics, 19(41), 1-12.
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2018 Tsai, T. -S., & St John, J. C. (2018). The effects of mitochondrial DNA supplementation at the time of fertilization on the gene expression profiles of porcine preimplantation embryos.. Molecular reproduction and development, 85(6), 490-504.
DOI Scopus2 WoS2 Europe PMC2
2018 Sun, X., Johnson, J., & St John, J. (2018). Global DNA methylation synergistically regulates the nuclear and mitochondrial genomes in glioblastoma cells. Nucleic Acids Research, 46(12), 5977-5995.
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2018 Tsai, T., Tyagi, S., & St John, J. (2018). The molecular characterisation of mitochondrial DNA deficient oocytes using a pig model. Human Reproduction, 33(5), 942-953.
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2018 Srirattana, K., & St John, J. (2018). Additional mitochondrial DNA influences the interactions between the nuclear and mitochondrial genomes in a bovine embryo model of nuclear transfer. Scientific Reports, 8(1), 17 pages.
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2018 St John, J., Srirattana, K., Tsai, T., & Sun, X. (2018). The mitochondrial genome: How it drives fertility. Reproduction, Fertility and Development, 30(1), 118-139.
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2017 Lee, W., Sun, X., Tsai, T., Johnson, J., Gould, J., Garama, D., . . . St John, J. (2017). Mitochondrial DNA haplotypes induce differential patterns of DNA methylation that result in differential chromosomal gene expression patterns. Cell Death Discovery, 3, 1-11.
2017 Srirattana, K., & St John, J. C. (2017). Manipulating the mitochondrial genome to enhance cattle embryo development. G3 : Genes, Genomes, Genetics, 7(7), 2065-2080.
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2017 Vaghjiani, V., Cain, J., Lee, W., Vaithilingam, V., Tuch, B., & St John, J. (2017). Modulation of Mitochondrial DNA Copy Number to Induce Hepatocytic Differentiation of Human Amniotic Epithelial Cells. Stem Cells and Development, 26(20), 1505-1519.
DOI Scopus1 Europe PMC1
2017 Tsai, T., Johnson, J., White, Y., & St John, J. (2017). The molecular characterization of porcine egg precursor cells. Oncotarget, 8(38), 63484-63505.
2017 Srirattana, K., McCosker, K., Schatz, T., & St John, J. (2017). Cattle phenotypes can disguise their maternal ancestry. BMC Genetics, 18(59), 11 pages.
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2016 Sun, X., & John, J. (2016). The role of the mtDNA set point in differentiation, development and tumorigenesis. Biochemical Journal, 473(19), 2955-2971.
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2016 Tsai, T., & St John, J. (2016). The role of mitochondrial DNA copy number, variants, and haplotypes in farm animal developmental outcome. Domestic Animal Endocrinology, 56, S133-S146.
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2016 Lee, W., & St John, J. (2016). Mitochondrial DNA as an initiator of tumorigenesis. Cell Death and Disease, 7(3), e2171.
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2016 Cagnone, G., Tsai, T., Srirattana, K., Rossello, F., Powell, D., Rohrer, G., . . . St John, J. (2016). Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics, 202(3), 931-944.
DOI Scopus12 Europe PMC9
2016 Tsai, T., Rajasekar, S., & St John, J. (2016). The relationship between mitochondrial DNA haplotype and the reproductive capacity of domestic pigs (Sus scrofa domesticus). BMC Genetics, 17(1), 67.
DOI Scopus18 Europe PMC9
2016 St John, J., Tsai, T., & Cagnone, G. (2016). Mitochondrial DNA supplementation as an enhancer of female reproductive capacity. Current Opinion in Obstetrics and Gynecology, 28(3), 211-216.
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2016 Cagnone, G., Tsai, T., Makanji, Y., Matthews, P., Gould, J., Bonkowski, M., . . . John, J. (2016). Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency. Scientific Reports, 6(1), 1-15.
DOI Scopus32 Europe PMC19
2016 St John, J. (2016). Mitochondrial DNA copy number and replication in reprogramming and differentiation. Seminars in Cell and Developmental Biology, 52, 93-101.
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2016 Johnson, J., Lee, W., Frazier, A., Vaghjiani, V., Laskowski, A., Rodriguez, A., . . . St John, J. (2016). Deletion of the Complex I Subunit NDUFS4 Adversely Modulates Cellular Differentiation. Stem Cells and Development, 25(3), 239-250.
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2015 Wu, L., Russell, D., Wong, S., Chen, M., Tsai, T., St John, J., . . . Robker, R. (2015). Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors. Development, 142(4), 681-691.
DOI Scopus93 WoS92 Europe PMC62
2015 Lee, W., Johnson, J., Gough, D., Donoghue, J., Cagnone, G., Vaghjiani, V., . . . St John, J. (2015). Mitochondrial DNA copy number is regulated by DNA methylation and demethylation of POLGA in stem and cancer cells and their differentiated progeny. Cell Death and Disease, 6(2), e1664.
DOI Scopus37 Europe PMC25
2015 Lee, W., & John, J. (2015). The control of mitochondrial DNA replication during development and tumorigenesis. Annals of the New York Academy of Sciences, 1350(1), 95-106.
2015 Henry, B., Loughnan, R., Hickford, J., Young, I., St John, J., & Clarke, I. (2015). Differences in mitochondrial DNA inheritance and function align with body conformation in genetically lean and fat sheep. Journal of Animal Science, 93(5), 2083-2093.
DOI Scopus6 Europe PMC3
2015 St John, J. (2015). The mitochondrion, its genome and their contribution to well-being and disease. Molecular Human Reproduction, 21(1), 1-2.
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2014 St John, J., Srirattana, K., & Clarke, I. (2014). Modifying the mitochondrial genome to enhance animal production. Australasian Biotechnology, 24(3), 61. 2014 Yeung, K., Dickinson, A., Donoghue, J., Polekhina, G., White, S., Grammatopoulos, D., . . . St John, J. (2014). The identification of mitochondrial DNA variants in glioblastoma multiforme. Acta Neuropathologica Communications, 2(1), 1.
DOI Scopus27 Europe PMC37
2014 Sobinoff, A., Sutherland, J., Beckett, E., Stanger, S., Johnson, R., Jarnicki, A., . . . Mclaughlin, E. (2014). Damaging legacy: Maternal cigarette smoking has long-term consequences for male offspring fertility. Human Reproduction, 29(12), 2719-2735.
DOI Scopus24 Europe PMC19
2014 St John, J. (2014). The control of mtDNA replication during differentiation and development. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1345-1354.
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2014 Nagley, P., St John, J., Gabriel, K., & McKenzie, M. (2014). Mitochondrial research in Australia: A major player in worldwide trends. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1225-1226.
2014 Mckenzie, M., Chiotis, M., Hroudová, J., Lopez Sanchez, M., Lim, S., Cook, M., . . . Trounce, I. (2014). Capture of somatic mtDNA point mutations with severe effects on oxidative phosphorylation in synaptosome cybrid clones from human brain. Human Mutation, 35(12), 1476-1484.
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2014 Lee, T., Clarke, I., St John, J., Young, I., Leury, B., Rao, A., . . . Henry, B. (2014). High cortisol responses identify propensity for obesity that is linked to thermogenesis in skeletal muscle. FASEB Journal, 28(1), 35-44.
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2013 Dickinson, A., Yeung, K., Donoghue, J., Baker, M., Kelly, R., McKenzie, M., . . . St John, J. (2013). The regulation of mitochondrial DNA copy number in glioblastoma cells. Cell Death and Differentiation, 20(12), 1644-1653.
DOI Scopus61 Europe PMC48
2013 Kelly, R., Rodda, A., Dickinson, A., Mahmud, A., Nefzger, C., Lee, W., . . . St John, J. (2013). Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells. Stem Cells, 31(4), 703-716.
DOI Scopus49 Europe PMC35
2013 Kelly, R., Sumer, H., McKenzie, M., Facucho-Oliveira, J., Trounce, I., Verma, P., & St John, J. (2013). The Effects of Nuclear Reprogramming on Mitochondrial DNA Replication. Stem Cell Reviews and Reports, 9(1), 1-15.
DOI Scopus36 Europe PMC23
2013 John, J. (2013). A tribute to somatic cell reprogrammers. Molecular Human Reproduction, 19(2), 55-56.
2012 Kelly, R., Mahmud, A., McKenzie, M., Trounce, I., & St John, J. (2012). Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A. Nucleic Acids Research, 40(20), 10124-10138.
DOI Scopus93 Europe PMC71
2012 St John, J. (2012). Transmission, inheritance and replication of mitochondrial DNA in mammals: Implications for reproductive processes and infertility. Cell and Tissue Research, 349(3), 795-808.
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2011 Rae, P., Kelly, R., Egginton, S., & St John, J. (2011). Angiogenic potential of endothelial progenitor cells and embryonic stem cells. Vascular Cell, 3(1), 11.
2011 Sanchez-Partida, L., Kelly, R., Sumer, H., Lo, C., Aharon, R., Holland, M., . . . John St, J. (2011). The generation of live offspring from vitrified oocytes. PLoS ONE, 6(6), e21597.
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2011 Kelly, R., & St John, J. (2011). Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells. International Journal of Developmental Biology, 54(11-12), 1659-1670.
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2011 Jiang, Y., Kelly, R., Peters, A., Fulka, H., Dickinson, A., Mitchell, D., & St John, J. (2011). Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors. PLoS ONE, 6(4), e14805.
DOI Scopus32 Europe PMC17
2010 St John, J., Facucho-Oliveira, J., Jiang, Y., Kelly, R., & Salah, R. (2010). Mitochondrial DNA transmission, replication and inheritance: A journey from the gamete through the embryo and into offspring and embryonic stem cells. Human Reproduction Update, 16(5), 488-509.
DOI Scopus138 Europe PMC87
2010 St John, J., & Campbell, K. (2010). The battle to prevent the transmission of mitochondrial DNA disease: Is karyoplast transfer the answer. Gene Therapy, 17(2), 147-149.
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2010 Lee, J., Peters, A., Fisher, P., Bowles, E., St John, J., & Campbell, K. (2010). Generation of mtDNA homoplasmic cloned lambs. Cellular Reprogramming, 12(3), 347-355.
DOI Scopus22 Europe PMC12
2009 Fulka, J., Loi, P., Ptak, G., Fulka, H., & John, J. (2009). Hope for the mammoth?. Cloning and Stem Cells, 11(1), 1-3.
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2009 Facucho-Oliveira, J., & John St, J. (2009). The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic Stem Cell differentiation. Stem Cell Reviews and Reports, 5(2), 140-158.
DOI Scopus160 Europe PMC109
2009 Taylor, R., Wang, H., Wilkinson, S., Richards, M., Britt, K., Vaillant, F., . . . Risbridger, G. (2009). Lineage enforcement by inductive mesenchyme on adult epithelial stem cells across developmental germ layers. Stem Cells, 27(12), 3032-3042.
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2008 Fulka, J., Fulka, H., St John, J., Galli, C., Lazzari, G., Lagutina, I., . . . Loi, P. (2008). Cybrid human embryos - warranting opportunities to augment embryonic stem cell research. Trends in Biotechnology, 26(9), 469-474.
DOI Scopus5 Europe PMC3
2008 Bowles, E., Tecirlioglu, R., French, A., Holland, M., & St John, J. (2008). Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells, 26(3), 775-782.
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2008 St John, J., Armstrong, L., Minger, S., & Campbell, K. (2008). Law should recognize value of interspecies embryos . Nature, 451(7179), 627.
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2007 Bowles, E., Lee, J., Alberio, R., Lloyd, R., Stekel, D., Campbell, K., & St John, J. (2007). Contrasting effects of in vitro fertilization and nuclear transfer on the expression of mtDNA replication factors. Genetics, 176(3), 1511-1526.
DOI Scopus47 Europe PMC31
2007 St John, J., Bowles, E., & Amaral, A. (2007). Sperm mitochondria and fertilisation.. Society of Reproduction and Fertility supplement, 65, 399-416.
Scopus17 Europe PMC9
2007 St John, J., & Lovell-Badge, R. (2007). Human-animal cytoplasmic hybrid embryos, mitochondria, and an energetic debate. Nature Cell Biology, 9(9), 988-992.
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2007 Spikings, E., Alderson, J., & St John, J. (2007). Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. Biology of Reproduction, 76(2), 327-335.
DOI Scopus158 Europe PMC112
2007 Amaral, A., Ramalho-Santos, J., & St John, J. (2007). The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Human Reproduction, 22(6), 1585-1596.
DOI Scopus74 Europe PMC43
2007 Facucho-Oliveira, J., Alderson, J., Spikings, E., Egginton, S., & St John, J. (2007). Mitochondrial DNA replication during differentiation of murine embryonic stem cells. Journal of Cell Science, 120(22), 4025-4034.
DOI Scopus198 Europe PMC154
2007 Fulka, J., Fulka, H., & St John, J. (2007). Transmission of mitochondrial DNA disorders: Possibilities for the elimination of mutated mitochondria. Cloning and Stem Cells, 9(1), 47-50.
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2006 Poulton, J., Kennedy, S., Oakeshott, P., & St John, J. (2006). Nuclear transfer to prevent mitochondrial DNA diseases. Lancet, 368(9538), 841.
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2006 Santos, T., El Shourbagy, S., & St John, J. (2006). Mitochondrial content reflects oocyte variability and fertilization outcome. Fertility and Sterility, 85(3), 584-591.
DOI Scopus198 Europe PMC137
2006 St John, J., Amaral, A., Bowles, E., Oliveira, J., Lloyd, R., Freitas, M., . . . Ramalho-Santos, J. (2006). The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells.. Methods in molecular biology (Clifton, N.J.), 331, 347-374.
DOI Scopus40 Europe PMC32
2006 El Shourbagy, S., Spikings, E., Freitas, M., & St John, J. (2006). Mitochondria directly influence fertilisation outcome in the pig. Reproduction, 131(2), 233-245.
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2006 Lloyd, R., Lee, J., Alberio, R., Bowles, E., Ramalho-Santos, J., Campbell, K., & St John, J. (2006). Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. Genetics, 172(4), 2515-2527.
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2006 Spikings, E., Alderson, J., & St John, J. (2006). Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer. Human Reproduction Update, 12(4), 401-415.
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2005 Fleming, C., Maldjian, A., Da Costa, D., Rullay, A., Haddleton, D., St John, J., . . . Davis, B. (2005). A Carbohydrate-Antioxidant Hybrid Polymer Reduces Oxidative Damage in Spermatozoa and Enhances Fertility. Nature Chemical Biology, 1(5), 270-274.
DOI Scopus58 Europe PMC17
2005 St John, J., & Alderson, J. (2005). Stem-cell banking: The size of the task. Lancet, 366(9502), 1991-1992.
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2005 St John, J., Ramalho-Santos, J., Gray, H., Petrosko, P., Rawe, V., Navara, C., . . . Schatten, G. (2005). The expression of mitochondrial DNA transcription factors during early cardiomyocyte in vitro differentiation from human embryonic stemn cells. Cloning and Stem Cells, 7(3), 141-153.
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2005 St John, J., Jokhi, R., & Barratt, C. (2005). The impact of mitochondrial genetics on male infertility. International Journal of Andrology, 28(2), 65-73.
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2005 St John, J., Moffatt, O., & D'Souza, N. (2005). Aberrant heteroplasmic transmission of mtDNA in cloned pigs arising from double nuclear transfer. Molecular Reproduction and Development, 72(4), 450-460.
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2004 St John, J., & Schatten, G. (2004). Paternal mitochondrial DNA transmission during nonhuman primate nuclear transfer. Genetics, 167(2), 897-905.
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2004 St John, J., Lloyd, R., Bowles, E., Thomas, E., & El Shourbagy, S. (2004). The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective. Reproduction, 127(6), 631-641.
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2004 Barratt, C., St John, J., & Afnan, M. (2004). Clinical challenges in providing embryos for stem-cell initiatives. Lancet, 364(9429), 115-118.
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2004 Mital, D., White, D., & St John, J. (2004). Mitochondrial DNA and sperm quality in patients on antiretroviral therapy - Response . AIDS, 18(6), 963.
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2004 St John, J., Lloyd, R., & El Shourbagy, S. (2004). The potential risks of abnormal transmission of mtDNA through assisted reproductive technologies. Reproductive BioMedicine Online, 8(1), 34-44.
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2003 Payne, C., St John, J., Ramalho-Santos, J., & Schatten, G. (2003). LIS1 Association with Dynactin is Required for Nuclear Motility and Genomic Union in the Fertilized Mammalian Oocyte. Cell Motility and the Cytoskeleton, 56(4), 245-251.
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2002 Perkins, J., St John, J., & Ahmed, A. (2002). Modulation of trophoblast cell death by oxygen and EGF. Molecular Medicine, 8(12), 847-856.
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2002 St John, J. (2002). Ooplasm donation in humans: The need to investigate the transmission of mitochondrial DNA following cytoplasmic transfer. Human Reproduction, 17(8), 1954-1958.
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2001 White, D., Mital, D., Taylor, S., & John, J. (2001). Sperm mitochondrial DNA deletions as a consequence of long term highly active antiretroviral therapy. AIDS, 15(8), 1061-1062.
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2001 John, J., Jokhi, R., & Barratt, C. (2001). Men with oligoasthenoteratozoospermia harbour higher numbers of multiple mitochondrial DNA deletions in their spermatozoa, but individual deletions are not indicative of overall aetiology. Molecular Human Reproduction, 7(1), 103-111.
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2000 John, J., & De Jonge, C. (2000). A hypothesis for transmission of paternal mitochondrial DNA. Reproductive Medicine Review, 8(1), 73-85.
2000 St John, J., Sakkas, D., & Barratt, C. (2000). A role for mitochondrial DNA and sperm survival. Journal of Andrology, 21(2), 189-199.
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2000 Ahmed, A., Rahman, M., Zhang, X., Acevedo, C., Nijjar, S., Rushton, I., . . . St John, J. (2000). Induction of placental heme oxygenase-1 is protective against TNFalpha-induced cytotoxicity and promotes vessel relaxation.. Molecular medicine (Cambridge, Mass.), 6(5), 391-409.
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2000 St John, J., Sakkas, D., Dimitriadi, K., Barnes, A., Maclin, V., Ramey, J., . . . De Jonge, C. (2000). Failure of elimination of paternal mitochondrial DNA in abnormal embryos. Lancet, 355(9199), 200.
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1999 St John, J. (1999). Incorporating molecular screening techniques into the modern andrology laboratory. Journal of Andrology, 20(6), 692-701.
1999 Sakkas, D., Mariethoz, E., & St John, J. (1999). Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the fas-mediated pathway. Experimental Cell Research, 251(2), 350-355.
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1997 St John, J., Cooke, I., Barratt, C., & Johns, D. (1997). Mitochondrial mutations and male infertility . Nature Medicine, 3(2), 124-125.
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1997 St John, J., Barratt, C., Brenner, C., Scott, J., & Cohen, J. (1997). Use of anucleate donor oocyte cytoplasm in recipient eggs (multiple letters) . Lancet, 350(9082), 961-962.
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Year Citation 2013 St John, J. (2013). Mitochondrial DNA, mitochondria, disease and stem cells.
2007 St John, J. (2007). Preface (Vol. 77).
2007 Bowles, E., Campbell, K., & St John, J. (2007). Nuclear Transfer: Preservation of a Nuclear Genome at the Expense of Its Associated mtDNA Genome(s) (Vol. 77).
DOI Scopus38 Europe PMC20
Year Citation 2019 Srirattana, K., & St John, J. (2019). Transmission of Dysfunctional Mitochondrial DNA and Its Implications for Mammalian Reproduction. In P. Sutovsky (Ed.), Advances in Anatomy Embryology and Cell Biology (Vol. 231, pp. 75-103). Switzerland: Springer Nature.
2016 Cagnone, G., Vaghjiani, V., Lee, W., Sun, C., Johnson, J., Yeung, K., & St John, J. (2016). Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. In K. Turksen, & A. Nagy (Eds.), Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Vol. 1357, pp. 231-267). New York; USA: Springer.
DOI Scopus1 Europe PMC1
2016 Sun, X., Lee, W., Vaghjiani, V., & St John, J. (2016). Analysis of mitochondrial DNA copy number and its regulation through DNA methylation of POLGA. In M. McKenzie (Ed.), Mitochondrial DNA: Methods and Protocols (Vol. 1351, pp. 131-141). New York; USA: Springer.
DOI Scopus1 Europe PMC1
2015 Lee, W., Kelly, R., Yeung, K., Cagnone, G., McKenzie, M., & St John, J. (2015). Analysis of mitochondrial DNA in induced pluripotent and embryonic stem cells. In P. J. Verma, & H. Sumer (Eds.), Cell Reprogramming: Methods and Protocols (Vol. 1330, pp. 219-252). New York; USA: Springer.
2013 St John, J. (2013). Mitochondrial DNA: Its Transmission from Gametes and Embryos. In Principles of Cloning: Second Edition (pp. 429-439).
2013 Kelly, R., Mahmud, A., & St John, J. (2013). Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 157-183).
2013 Yeung, K., Dickinson, A., & St John, J. (2013). The Role of Mitochondrial DNA in Tumorigenesis. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 119-155).
2013 Facucho-Oliveira, J., Kulkarni, T., Machado-Oliveira, G., & St John, J. (2013). From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 87-118).
2012 St John, J., & Campbell, K. (2012). The consequences of reprogramming a somatic cell for mitochondrial DNA transmission, inheritance and replication. In Nuclear Reprogramming and Stem Cells (pp. 83-97).
Year Citation 2009 St John, J. (2009). mtDNA and sperm function. In Systems Biology in Reproductive Medicine Vol. 55 (pp. 17-18). 2008 Fulka, H., St John, J., Fulka, J., & Hozák, P. (2008). Chromatin in early mammalian embryos: Achieving the pluripotent state. In Differentiation Vol. 76 (pp. 3-14). England.
DOI Scopus45 Europe PMC27
2002 St John, J. (2002). The transmission of mitochondrial DNA following assisted reproductive techniques. In Theriogenology Vol. 57 (pp. 109-123). United States.
DOI Scopus39 Europe PMC23
1998 Barratt, C., & St John, J. (1998). Diagnostic tools in male infertility. In Human Reproduction Vol. 13 (pp. 51-61). England.
DOI Scopus13 Europe PMC3
Year Citation 2014 Chang, J., Chang, C., Chao, S., Cheng, A., Dai, K., Lee, J., . . . Yang, C. (2014). Preface. Poster session presented at the meeting of Geotechnical Special Publication.
2019 – 2022: NHMRC Project Grant (GNT1160106), Understanding how mitochondrial DNA contributes to embryo development (St. John J) $669,790
2018 – 2020: NHMRC Development Grant (GNT1136065), Improving oocyte mitochondrial DNA copy number to enhance female reproductive capacity (St. John J, McKenzie W, Western P, Rombauts L) $670,867.20.
2017 – 2018: The Winston Foundation (WF1), Developing a canine in vitro maturation culture system (St. John J) $42,000
2016 – 2018: OvaScience Inc. Waltham, USA. Renewal Grant, Safety of Mitochondrial Transfer (St. John J) $600,000.
2016 – 2019: ARC Discovery Grant(DP160102575), Diet influences the selective advantage of mitochondrial DNA mutations (Ballard B, St. John J, Smyth G) $313,600.00
2015 – 2017: NHMRC Project Grant(GNT1079309) - Metabolic and molecular basis of embryo signalling (Lane M, St. John J, Zander-Fox D) $397,077.00
2014 – 2016: Australian Mitochondrial Disease Foundation- Preventing the transmission of mutant mitochondrial DNA (St. John J) $25,000
2013 – 2016: OvaScience Inc- Determining the safety of homologous mitochondrial transfer in a mini-pig model (St. John J) $1,250,000
2013 – 2015: NHMRC Project Grant(GNT1041471) - Understanding mitochondrial DNA segregation and transmission (CIA - St. John J; Trounce I) $492,863
2013 – 2015: Zwart Estate- The role of mtDNA variants in glioblastoma (St. John J) $133,131
2013 – 2016: Australian Pork Limited- Defining the role of mitochondrial DNA in pig fertility (St. John J) $92,637
2013 – 2014: Johnston Estate- The generation of stem cell models of mtDNA disease (St. John J) $170,000
2012 – 2014: NHMRC Project Grant(GNT1022222) - Understanding the pathogenesis of mitochondrial disease using iPS cells (Thorburn D, St. John J, Frazier A, Nisbet D, White S) $618,675
2011: Marian and EH Flack Trust- Funding to purchase a High Resolution Melting Curve Analysis Machine(White S, Western P, St. John J, Watkins DN) $30,000.
Current Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2019 Co-Supervisor Increasing Mitochondrial DNA Copy Number to Enhance Female Reproductive Capacity Doctor of Philosophy Doctorate Full Time Dr Dang Kien Nguyen
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