Jus St John

Professor Jus St John

Professor

Adelaide Medical School

Faculty of Health and Medical Sciences

Eligible to supervise Masters and PhD - email supervisor to discuss availability.


The overall aim of my research program is to understand how the nuclear and mitochondrial genomes interact during development in order that cells, tissues and organs function efficiently. We undertake these studies using oocytes, preimplantation embryos, embryonic and adult stem cells, somatic cells, and tumour-initiating cells. We use a number of oocyte, embryo and cell manipulation techniques, next generation sequencing technologies and bioinformatics. Our work is divided into three areas, which we will continue to interrogate over the next five years.

Research Focus One: Understanding the role of mitochondrial DNA (mtDNA) in oocyte quality and development outcomes.

We undertake this area of research using porcine and bovine oocytes and generate embryos and offspring through in vitrofertilisation (IVF), nuclear transfer (NT) cytoplasmic transfer (CT), and mitochondrial supplementation (mICSI).  This enables us to determine how the nuclear and mtDNA content of an oocyte affects fertilisation and developmental outcome; and how mtDNA segregation, transmission and replication are normally regulated, as is the case following IVF, and perturbed as these mechanisms are violated by NT and CT.

 

We are currently funded by the NHMRC (Development Grant) to generate mini-pig models using a new assisted reproductive technology that we have been developing, which involves supplementing oocytes deficient in mtDNA with genetically identical populations of mtDNA. This technology is known as mICSI,and our published data suggest that mICSI enhances embryo quality and overcomes the predisposition of poor quality oocytes to give rise to diabetes and obesity. The grant will determine whether supplementing eggs at the time of fertilisation with genetically identical mtDNA is safe practice. Furthermore, we are also funded through an NHMRC Project Grant to determine the mechanisms associated with mICSI. 

 

We have also previously generated a derivation of another assisted reproductive technology, namely cloning or somatic cell nuclear transfer (SCNT), that ensures cloned embryos inherit their mtDNA from the population present in the oocyte only, as is the case following natural conception, and not from the somatic cell as well. This technology, known as mito-SCNT, improves embryo quality and developmental outcomes and overcomes the problem of embryos and offspring inheriting two populations of mtDNA.

 

Research Focus Two: Defining the role of the mtDNA set point in early development and cancer.

In recent years, we have discovered the ‘mtDNA set point’, which is a key developmental milestone that naïve (pluripotent) cells must acquire in order to initiate and complete their differentiation into mature cell types. The mtDNA set point is characterised by cells expressing pluripotent markers and having very low mtDNA copy number, which are both regulated by the epigenetic status of the nuclear genome. Furthermore, we have shown that key nuclear-encoded mtDNA replication factors are DNA methylated in a tissue specific manner that accounts for cell-specific mtDNA copy number, which requires the establishment of the mtDNA set point early during development. Our studies have also shown that failure to establish the mtDNA set point results in developmental failure and is a key characteristic of tumour-initiating cells. In addition, by depleting tumour-initiating cells of their mtDNA, we have shown that the incidence of tumour formation is significantly reduced (glioblastoma mutiforme and osteosarcoma) or prevented (multiple myeloma), as these cells re-establish the mtDNA set point and undergo differentiation and, therefore, do not form tumours.

 

We use and derive embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and tumour-initiating cell lines and tumours to perform these studies. These models allow us to focus on molecular mechanisms and to identify the specific windows during differentiation/development when these events manifest and then to confirm and characterise these events through, for example, targeted gene knockdown and overexpression.

 

Research Focus Three: Determining how mtDNA haplotypes influence phenotype. 

Recent outcomes from our research program have shown that mtDNA halpotypes influence livestock phenotypes, such as meat quality and lifetime daily gains in pigs, and reproductive efficiencies in pigs and cattle. Using stem cells models, we have been able to demonstrate that mtDNA haplotypes induce phenotypic changes through modulation of DNA methylation patterns that, in turn, affect chromosomal gene expression patterns. Using tumour cells, we have also demonstrated differences in the onset and progression of tumours based on mtDNA haplotype alone.

CURRENT RESEARCH PROJECTS AVAILABLE FOR STUDENTS:

RESEARCH PROJECT 1: Identifying variants in the mitochondrial genome predictive of oocyte quality.

Project description: A number of mitochondrial DNA variants have been identified in oocytes. However, their affect and role have yet to be properly defined, especially in oocytes from offspring derived from assisted reproductive technologies that perturb the maternal only transmission of mitochondrial DNA. Using next generation sequencing and bioinformatics tools, this project will focus on identifying variants in the mitochondrial genome that are predictive of oocyte quality and whether an oocyte will progress to fertilise and develop into an embryo. Using an experimental model of pig oocytein vitromaturation, you will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitrofertilisation protocols. After preparing fertilised and failed to fertilise oocyte samples for next generation mitochondrial DNA sequencing technology, you will learn to apply bioinformatics tools to identify and quantify variants that are associated with good and poor oocyte quality. These outputs will then be tested in: i) a larger cohort of oocytes from commercial breeding populations and matched to data associated with economic breeding values; and ii) oocytes derived from offspring that were generated from assisted reproductive technologies that result in two populations of mitochondrial DNA being transmitted. 

Projects available for:Honours / HDR / Masters / Mphil / PhD

Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Level 5.

Research project start: Semester 1 and 2

Special requirements: Vaccination required.

 

RESEARCH PROJECT 2: Identifying global DNA methylation patterns associated in the preimplantation embryo.

Project description: With the introduction of more invasive assisted reproductive technologies into animal breeding programs and their potential implementation into human clinical IVF, we need to understand how and when they modify the epigenome during early development, especially technologies that perturb the balance between the nuclear and mitochondrial genomes. DNA methylation is a key epigenetic mechanism that regulates gene expression during early development.  Using a porcine oocyte and embryo in vitromodel, next generation bisulfite DNA sequencing and bioinformatics tools, this project will focus on mapping the DNA methylation profiles of oocytes and early embryos to define the waves of DNA methylation prior to and post-embryonic genome activation, the time point when the early embryo initiates its own global transcription. You will learn how to harvest oocytes from ovaries; culture these immature oocytes to a mature stage; and fertilise them using in vitrofertilisation protocols. After preparing fertilised oocyte and embryo samples for next generation bisulfite DNA sequencing, you will learn to apply bioinformatics tools to map regions of DNA methylation across the whole genome and validate the findings. These outputs will then be tested against embryos generated from other assisted reproductive technologies to determine how these technologies impact on the DNA methylation profiles of the developing embryo.

Projects available for: Third Year / Honours / HDR / Masters / Mphil / PhD

Location: AHMS (Adelaide Health & Medical Sciences Building, North Tce) – Level 5.

Research project start: Semester 1 and 2

Special requirements: Vaccination required.

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  • Journals

    Year Citation
    2019 St John, J. C. (2019). Mitochondria and Female Germline Stem Cells-A Mitochondrial DNA Perspective.. Cells, 8(8).
    DOI
    2019 St John, J., Makanji, Y., Johnson, J., Tsai, T., Lagondar, S., Rodda, F., . . . Temple-Smith, P. (2019). The transgenerational effects of oocyte mitochondrial supplementation. Scientific Reports, 9(1), 12 pages.
    DOI
    2018 Sun, X., Vaghjiani, V., Jayasekara, W. S. N., Cain, J. E., & St John, J. C. (2018). The degree of mitochondrial DNA methylation in tumor models of glioblastoma and osteosarcoma.. Clinical epigenetics, 10(1), 157.
    DOI
    2018 Sun, X., & St John, J. C. (2018). Modulation of mitochondrial DNA copy number in a model of glioblastoma induces changes to DNA methylation and gene expression of the nuclear genome in tumours. Epigenetics & chromatin, 11(1), 53.
    DOI Scopus1 WoS1
    2018 St John, J. C., & Tsai, T. -S. (2018). The association of mitochondrial DNA haplotypes and phenotypic traits in pigs.. BMC genetics, 19(41), 1-12.
    DOI Scopus2 WoS1 Europe PMC1
    2018 Tsai, T. -S., & St John, J. C. (2018). The effects of mitochondrial DNA supplementation at the time of fertilization on the gene expression profiles of porcine preimplantation embryos.. Molecular reproduction and development, 85(6), 490-504.
    DOI Scopus2 WoS2 Europe PMC1
    2018 Sun, X., Johnson, J., & St John, J. (2018). Global DNA methylation synergistically regulates the nuclear and mitochondrial genomes in glioblastoma cells. Nucleic Acids Research, 46(12), 5977-5995.
    DOI Scopus8 Europe PMC3
    2018 Tsai, T., Tyagi, S., & St John, J. (2018). The molecular characterisation of mitochondrial DNA deficient oocytes using a pig model. Human Reproduction, 33(5), 942-953.
    DOI Scopus4 Europe PMC2
    2018 Srirattana, K., & St John, J. (2018). Additional mitochondrial DNA influences the interactions between the nuclear and mitochondrial genomes in a bovine embryo model of nuclear transfer. Scientific Reports, 8(1), 17 pages.
    DOI Scopus3
    2018 St John, J., Srirattana, K., Tsai, T., & Sun, X. (2018). The mitochondrial genome: How it drives fertility. Reproduction, Fertility and Development, 30(1), 118-139.
    DOI
    2017 Lee, W., Sun, X., Tsai, T., Johnson, J., Gould, J., Garama, D., . . . St John, J. (2017). Mitochondrial DNA haplotypes induce differential patterns of DNA methylation that result in differential chromosomal gene expression patterns. Cell Death Discovery, 3, 1-11.
    DOI Scopus7
    2017 Srirattana, K., & St John, J. C. (2017). Manipulating the mitochondrial genome to enhance cattle embryo development. G3 : Genes, Genomes, Genetics, 7(7), 2065-2080.
    DOI Scopus4 WoS3 Europe PMC2
    2017 Vaghjiani, V., Cain, J., Lee, W., Vaithilingam, V., Tuch, B., & St John, J. (2017). Modulation of Mitochondrial DNA Copy Number to Induce Hepatocytic Differentiation of Human Amniotic Epithelial Cells. Stem Cells and Development, 26(20), 1505-1519.
    DOI Scopus1 Europe PMC1
    2017 Tsai, T., Johnson, J., White, Y., & St John, J. (2017). The molecular characterization of porcine egg precursor cells. Oncotarget, 8(38), 63484-63505.
    DOI Scopus2
    2017 Srirattana, K., McCosker, K., Schatz, T., & St John, J. (2017). Cattle phenotypes can disguise their maternal ancestry. BMC Genetics, 18(59), 11 pages.
    DOI Scopus7 Europe PMC3
    2016 Sun, X., & John, J. (2016). The role of the mtDNA set point in differentiation, development and tumorigenesis. Biochemical Journal, 473(19), 2955-2971.
    DOI Scopus10 Europe PMC5
    2016 Tsai, T., & St John, J. (2016). The role of mitochondrial DNA copy number, variants, and haplotypes in farm animal developmental outcome. Domestic Animal Endocrinology, 56, S133-S146.
    DOI Scopus7 Europe PMC2
    2016 Lee, W., & St John, J. (2016). Mitochondrial DNA as an initiator of tumorigenesis. Cell Death and Disease, 7(3), e2171.
    DOI Scopus4 Europe PMC3
    2016 Cagnone, G., Tsai, T., Srirattana, K., Rossello, F., Powell, D., Rohrer, G., . . . St John, J. (2016). Segregation of naturally occurring mitochondrial DNA variants in a mini-pig model. Genetics, 202(3), 931-944.
    DOI Scopus10 Europe PMC8
    2016 Tsai, T., Rajasekar, S., & St John, J. (2016). The relationship between mitochondrial DNA haplotype and the reproductive capacity of domestic pigs (Sus scrofa domesticus). BMC Genetics, 17(1), 67.
    DOI Scopus15 Europe PMC8
    2016 St John, J., Tsai, T., & Cagnone, G. (2016). Mitochondrial DNA supplementation as an enhancer of female reproductive capacity. Current Opinion in Obstetrics and Gynecology, 28(3), 211-216.
    DOI Scopus3 Europe PMC2
    2016 Cagnone, G., Tsai, T., Makanji, Y., Matthews, P., Gould, J., Bonkowski, M., . . . John, J. (2016). Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency. Scientific Reports, 6(1), 1-15.
    DOI Scopus30 Europe PMC18
    2016 St John, J. (2016). Mitochondrial DNA copy number and replication in reprogramming and differentiation. Seminars in Cell and Developmental Biology, 52, 93-101.
    DOI Scopus21 Europe PMC13
    2016 Johnson, J., Lee, W., Frazier, A., Vaghjiani, V., Laskowski, A., Rodriguez, A., . . . St John, J. (2016). Deletion of the Complex I Subunit NDUFS4 Adversely Modulates Cellular Differentiation. Stem Cells and Development, 25(3), 239-250.
    DOI Scopus4 Europe PMC1
    2015 Lee, W., Johnson, J., Gough, D., Donoghue, J., Cagnone, G., Vaghjiani, V., . . . St John, J. (2015). Mitochondrial DNA copy number is regulated by DNA methylation and demethylation of POLGA in stem and cancer cells and their differentiated progeny. Cell Death and Disease, 6(2), e1664.
    DOI Scopus36 Europe PMC23
    2015 Lee, W., & John, J. (2015). The control of mitochondrial DNA replication during development and tumorigenesis. Annals of the New York Academy of Sciences, 1350(1), 95-106.
    DOI Scopus22
    2015 Henry, B., Loughnan, R., Hickford, J., Young, I., St John, J., & Clarke, I. (2015). Differences in mitochondrial DNA inheritance and function align with body conformation in genetically lean and fat sheep. Journal of Animal Science, 93(5), 2083-2093.
    DOI Scopus5 Europe PMC3
    2015 Wu, L., Russell, D., Wong, S., Chen, M., Tsai, T., St John, J., . . . Robker, R. (2015). Mitochondrial dysfunction in oocytes of obese mothers: transmission to offspring and reversal by pharmacological endoplasmic reticulum stress inhibitors. Development, 142(4), 681-691.
    DOI Scopus87 WoS84 Europe PMC56
    2015 St John, J. (2015). The mitochondrion, its genome and their contribution to well-being and disease. Molecular Human Reproduction, 21(1), 1-2.
    DOI Scopus3 Europe PMC4
    2014 St John, J., Srirattana, K., & Clarke, I. (2014). Modifying the mitochondrial genome to enhance animal production. Australasian Biotechnology, 24(3), 61.
    2014 Yeung, K., Dickinson, A., Donoghue, J., Polekhina, G., White, S., Grammatopoulos, D., . . . St John, J. (2014). The identification of mitochondrial DNA variants in glioblastoma multiforme. Acta Neuropathologica Communications, 2(1), 1.
    DOI Scopus26 Europe PMC33
    2014 Sobinoff, A., Sutherland, J., Beckett, E., Stanger, S., Johnson, R., Jarnicki, A., . . . Mclaughlin, E. (2014). Damaging legacy: Maternal cigarette smoking has long-term consequences for male offspring fertility. Human Reproduction, 29(12), 2719-2735.
    DOI Scopus24 Europe PMC19
    2014 St John, J. (2014). The control of mtDNA replication during differentiation and development. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1345-1354.
    DOI Scopus48 Europe PMC29
    2014 Nagley, P., St John, J., Gabriel, K., & McKenzie, M. (2014). Mitochondrial research in Australia: A major player in worldwide trends. Biochimica et Biophysica Acta - General Subjects, 1840(4), 1225-1226.
    DOI
    2014 Mckenzie, M., Chiotis, M., Hroudová, J., Lopez Sanchez, M., Lim, S., Cook, M., . . . Trounce, I. (2014). Capture of somatic mtDNA point mutations with severe effects on oxidative phosphorylation in synaptosome cybrid clones from human brain. Human Mutation, 35(12), 1476-1484.
    DOI Scopus7 Europe PMC6
    2014 Lee, T., Clarke, I., St John, J., Young, I., Leury, B., Rao, A., . . . Henry, B. (2014). High cortisol responses identify propensity for obesity that is linked to thermogenesis in skeletal muscle. FASEB Journal, 28(1), 35-44.
    DOI Scopus11 Europe PMC7
    2013 Dickinson, A., Yeung, K., Donoghue, J., Baker, M., Kelly, R., McKenzie, M., . . . St John, J. (2013). The regulation of mitochondrial DNA copy number in glioblastoma cells. Cell Death and Differentiation, 20(12), 1644-1653.
    DOI Scopus60 Europe PMC47
    2013 Kelly, R., Rodda, A., Dickinson, A., Mahmud, A., Nefzger, C., Lee, W., . . . St John, J. (2013). Mitochondrial DNA haplotypes define gene expression patterns in pluripotent and differentiating embryonic stem cells. Stem Cells, 31(4), 703-716.
    DOI Scopus48 Europe PMC33
    2013 Kelly, R., Sumer, H., McKenzie, M., Facucho-Oliveira, J., Trounce, I., Verma, P., & St John, J. (2013). The Effects of Nuclear Reprogramming on Mitochondrial DNA Replication. Stem Cell Reviews and Reports, 9(1), 1-15.
    DOI Scopus36 Europe PMC22
    2013 John, J. (2013). A tribute to somatic cell reprogrammers. Molecular Human Reproduction, 19(2), 55-56.
    DOI
    2012 Kelly, R., Mahmud, A., McKenzie, M., Trounce, I., & St John, J. (2012). Mitochondrial DNA copy number is regulated in a tissue specific manner by DNA methylation of the nuclear-encoded DNA polymerase gamma A. Nucleic Acids Research, 40(20), 10124-10138.
    DOI Scopus90 Europe PMC68
    2012 St John, J. (2012). Transmission, inheritance and replication of mitochondrial DNA in mammals: Implications for reproductive processes and infertility. Cell and Tissue Research, 349(3), 795-808.
    DOI Scopus20 Europe PMC6
    2011 Rae, P., Kelly, R., Egginton, S., & St John, J. (2011). Angiogenic potential of endothelial progenitor cells and embryonic stem cells. Vascular Cell, 3(1), 11.
    DOI Scopus25
    2011 Sanchez-Partida, L., Kelly, R., Sumer, H., Lo, C., Aharon, R., Holland, M., . . . John St, J. (2011). The generation of live offspring from vitrified oocytes. PLoS ONE, 6(6), e21597.
    DOI Scopus12 WoS10 Europe PMC9
    2011 Jiang, Y., Kelly, R., Peters, A., Fulka, H., Dickinson, A., Mitchell, D., & St John, J. (2011). Interspecies somatic cell nuclear transfer is dependent on compatible mitochondrial DNA and reprogramming factors. PLoS ONE, 6(4), e14805.
    DOI Scopus31 Europe PMC16
    2011 Kelly, R., & St John, J. (2011). Role of mitochondrial DNA replication during differentiation of reprogrammed stem cells. International Journal of Developmental Biology, 54(11-12), 1659-1670.
    DOI Scopus16 Europe PMC11
    2010 St John, J., & Campbell, K. (2010). The battle to prevent the transmission of mitochondrial DNA disease: Is karyoplast transfer the answer. Gene Therapy, 17(2), 147-149.
    DOI Scopus16 Europe PMC8
    2010 St John, J., Facucho-Oliveira, J., Jiang, Y., Kelly, R., & Salah, R. (2010). Mitochondrial DNA transmission, replication and inheritance: A journey from the gamete through the embryo and into offspring and embryonic stem cells. Human Reproduction Update, 16(5), 488-509.
    DOI Scopus136 Europe PMC85
    2010 Lee, J., Peters, A., Fisher, P., Bowles, E., St John, J., & Campbell, K. (2010). Generation of mtDNA homoplasmic cloned lambs. Cellular Reprogramming, 12(3), 347-355.
    DOI Scopus22 Europe PMC12
    2009 Fulka, J., Loi, P., Ptak, G., Fulka, H., & John, J. (2009). Hope for the mammoth?. Cloning and Stem Cells, 11(1), 1-3.
    DOI Scopus6 Europe PMC9
    2009 Facucho-Oliveira, J., & John St, J. (2009). The relationship between pluripotency and mitochondrial DNA proliferation during early embryo development and embryonic Stem Cell differentiation. Stem Cell Reviews and Reports, 5(2), 140-158.
    DOI Scopus158 Europe PMC106
    2009 Taylor, R., Wang, H., Wilkinson, S., Richards, M., Britt, K., Vaillant, F., . . . Risbridger, G. (2009). Lineage enforcement by inductive mesenchyme on adult epithelial stem cells across developmental germ layers. Stem Cells, 27(12), 3032-3042.
    DOI Scopus21 Europe PMC16
    2008 Fulka, J., Fulka, H., St John, J., Galli, C., Lazzari, G., Lagutina, I., . . . Loi, P. (2008). Cybrid human embryos - warranting opportunities to augment embryonic stem cell research. Trends in Biotechnology, 26(9), 469-474.
    DOI Scopus5 Europe PMC3
    2008 Bowles, E., Tecirlioglu, R., French, A., Holland, M., & St John, J. (2008). Mitochondrial DNA transmission and transcription after somatic cell fusion to one or more cytoplasts. Stem Cells, 26(3), 775-782.
    DOI Scopus37 Europe PMC22
    2008 St John, J., Armstrong, L., Minger, S., & Campbell, K. (2008). Law should recognize value of interspecies embryos [1]. Nature, 451(7179), 627.
    DOI Scopus5 Europe PMC2
    2007 St John, J., & Lovell-Badge, R. (2007). Human-animal cytoplasmic hybrid embryos, mitochondria, and an energetic debate. Nature Cell Biology, 9(9), 988-992.
    DOI Scopus21 Europe PMC14
    2007 Bowles, E., Lee, J., Alberio, R., Lloyd, R., Stekel, D., Campbell, K., & St John, J. (2007). Contrasting effects of in vitro fertilization and nuclear transfer on the expression of mtDNA replication factors. Genetics, 176(3), 1511-1526.
    DOI Scopus47 Europe PMC30
    2007 St John, J., Bowles, E., & Amaral, A. (2007). Sperm mitochondria and fertilisation.. Society of Reproduction and Fertility supplement, 65, 399-416.
    Scopus16 Europe PMC8
    2007 Spikings, E., Alderson, J., & St John, J. (2007). Regulated mitochondrial DNA replication during oocyte maturation is essential for successful porcine embryonic development. Biology of Reproduction, 76(2), 327-335.
    DOI Scopus157 Europe PMC111
    2007 Amaral, A., Ramalho-Santos, J., & St John, J. (2007). The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm. Human Reproduction, 22(6), 1585-1596.
    DOI Scopus74 Europe PMC42
    2007 Facucho-Oliveira, J., Alderson, J., Spikings, E., Egginton, S., & St John, J. (2007). Mitochondrial DNA replication during differentiation of murine embryonic stem cells. Journal of Cell Science, 120(22), 4025-4034.
    DOI Scopus194 Europe PMC150
    2007 Fulka, J., Fulka, H., & St John, J. (2007). Transmission of mitochondrial DNA disorders: Possibilities for the elimination of mutated mitochondria. Cloning and Stem Cells, 9(1), 47-50.
    DOI Scopus6 Europe PMC3
    2006 Spikings, E., Alderson, J., & St John, J. (2006). Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer. Human Reproduction Update, 12(4), 401-415.
    DOI Scopus55 Europe PMC32
    2006 Poulton, J., Kennedy, S., Oakeshott, P., & St John, J. (2006). Nuclear transfer to prevent mitochondrial DNA diseases. Lancet, 368(9538), 841.
    DOI Scopus8 Europe PMC2
    2006 Santos, T., El Shourbagy, S., & St John, J. (2006). Mitochondrial content reflects oocyte variability and fertilization outcome. Fertility and Sterility, 85(3), 584-591.
    DOI Scopus196 Europe PMC135
    2006 Lloyd, R., Lee, J., Alberio, R., Bowles, E., Ramalho-Santos, J., Campbell, K., & St John, J. (2006). Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos. Genetics, 172(4), 2515-2527.
    DOI Scopus52 Europe PMC34
    2006 St John, J., Amaral, A., Bowles, E., Oliveira, J., Lloyd, R., Freitas, M., . . . Ramalho-Santos, J. (2006). The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells.. Methods in molecular biology (Clifton, N.J.), 331, 347-374.
    DOI Scopus39 Europe PMC32
    2006 El Shourbagy, S., Spikings, E., Freitas, M., & St John, J. (2006). Mitochondria directly influence fertilisation outcome in the pig. Reproduction, 131(2), 233-245.
    DOI Scopus217 Europe PMC149
    2005 Fleming, C., Maldjian, A., Da Costa, D., Rullay, A., Haddleton, D., St John, J., . . . Davis, B. (2005). A Carbohydrate-Antioxidant Hybrid Polymer Reduces Oxidative Damage in Spermatozoa and Enhances Fertility. Nature Chemical Biology, 1(5), 270-274.
    DOI Scopus58 Europe PMC17
    2005 St John, J., Moffatt, O., & D'Souza, N. (2005). Aberrant heteroplasmic transmission of mtDNA in cloned pigs arising from double nuclear transfer. Molecular Reproduction and Development, 72(4), 450-460.
    DOI Scopus30 Europe PMC20
    2005 St John, J., & Alderson, J. (2005). Stem-cell banking: The size of the task. Lancet, 366(9502), 1991-1992.
    DOI Scopus3 Europe PMC2
    2005 St John, J., Ramalho-Santos, J., Gray, H., Petrosko, P., Rawe, V., Navara, C., . . . Schatten, G. (2005). The expression of mitochondrial DNA transcription factors during early cardiomyocyte in vitro differentiation from human embryonic stemn cells. Cloning and Stem Cells, 7(3), 141-153.
    DOI Scopus179 Europe PMC133
    2005 St John, J., Jokhi, R., & Barratt, C. (2005). The impact of mitochondrial genetics on male infertility. International Journal of Andrology, 28(2), 65-73.
    DOI Scopus74 Europe PMC45
    2004 St John, J., & Schatten, G. (2004). Paternal mitochondrial DNA transmission during nonhuman primate nuclear transfer. Genetics, 167(2), 897-905.
    DOI Scopus52 Europe PMC25
    2004 St John, J., Lloyd, R., Bowles, E., Thomas, E., & El Shourbagy, S. (2004). The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective. Reproduction, 127(6), 631-641.
    DOI Scopus69 Europe PMC39
    2004 Mital, D., White, D., & St John, J. (2004). Mitochondrial DNA and sperm quality in patients on antiretroviral therapy - Response [3]. AIDS, 18(6), 963.
    DOI Scopus4 Europe PMC2
    2004 Barratt, C., St John, J., & Afnan, M. (2004). Clinical challenges in providing embryos for stem-cell initiatives. Lancet, 364(9429), 115-118.
    DOI Scopus19 Europe PMC12
    2004 St John, J., Lloyd, R., & El Shourbagy, S. (2004). The potential risks of abnormal transmission of mtDNA through assisted reproductive technologies. Reproductive BioMedicine Online, 8(1), 34-44.
    DOI Scopus27 Europe PMC10
    2003 Payne, C., St John, J., Ramalho-Santos, J., & Schatten, G. (2003). LIS1 Association with Dynactin is Required for Nuclear Motility and Genomic Union in the Fertilized Mammalian Oocyte. Cell Motility and the Cytoskeleton, 56(4), 245-251.
    DOI Scopus14 Europe PMC10
    2002 Perkins, J., St John, J., & Ahmed, A. (2002). Modulation of trophoblast cell death by oxygen and EGF. Molecular Medicine, 8(12), 847-856.
    DOI Scopus23 Europe PMC16
    2002 St John, J. (2002). Ooplasm donation in humans: The need to investigate the transmission of mitochondrial DNA following cytoplasmic transfer. Human Reproduction, 17(8), 1954-1958.
    DOI Scopus46 Europe PMC26
    2001 White, D., Mital, D., Taylor, S., & John, J. (2001). Sperm mitochondrial DNA deletions as a consequence of long term highly active antiretroviral therapy. AIDS, 15(8), 1061-1062.
    DOI Scopus50 Europe PMC22
    2001 John, J., Jokhi, R., & Barratt, C. (2001). Men with oligoasthenoteratozoospermia harbour higher numbers of multiple mitochondrial DNA deletions in their spermatozoa, but individual deletions are not indicative of overall aetiology. Molecular Human Reproduction, 7(1), 103-111.
    DOI Scopus75 Europe PMC45
    2000 John, J., & De Jonge, C. (2000). A hypothesis for transmission of paternal mitochondrial DNA. Reproductive Medicine Review, 8(1), 73-85.
    DOI Scopus6
    2000 St John, J., Sakkas, D., & Barratt, C. (2000). A role for mitochondrial DNA and sperm survival. Journal of Andrology, 21(2), 189-199.
    Scopus44 Europe PMC24
    2000 Ahmed, A., Rahman, M., Zhang, X., Acevedo, C., Nijjar, S., Rushton, I., . . . St John, J. (2000). Induction of placental heme oxygenase-1 is protective against TNFalpha-induced cytotoxicity and promotes vessel relaxation.. Molecular medicine (Cambridge, Mass.), 6(5), 391-409.
    DOI Scopus110 Europe PMC85
    2000 St John, J., Sakkas, D., Dimitriadi, K., Barnes, A., Maclin, V., Ramey, J., . . . De Jonge, C. (2000). Failure of elimination of paternal mitochondrial DNA in abnormal embryos. Lancet, 355(9199), 200.
    DOI Scopus67 Europe PMC31
    1999 St John, J. (1999). Incorporating molecular screening techniques into the modern andrology laboratory. Journal of Andrology, 20(6), 692-701.
    Scopus8
    1999 Sakkas, D., Mariethoz, E., & St John, J. (1999). Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the fas-mediated pathway. Experimental Cell Research, 251(2), 350-355.
    DOI Scopus259 Europe PMC144
    1997 St John, J., Cooke, I., Barratt, C., & Johns, D. (1997). Mitochondrial mutations and male infertility [2]. Nature Medicine, 3(2), 124-125.
    DOI Scopus54 Europe PMC25
    1997 St John, J., Barratt, C., Brenner, C., Scott, J., & Cohen, J. (1997). Use of anucleate donor oocyte cytoplasm in recipient eggs (multiple letters) [16]. Lancet, 350(9082), 961-962.
    DOI Scopus12 Europe PMC7
  • Books

    Year Citation
    2013 St John, J. (2013). Mitochondrial DNA, mitochondria, disease and stem cells.
    DOI
    2007 St John, J. (2007). Preface (Vol. 77).
    DOI
    2007 Bowles, E., Campbell, K., & St John, J. (2007). Nuclear Transfer: Preservation of a Nuclear Genome at the Expense of Its Associated mtDNA Genome(s) (Vol. 77).
    DOI Scopus38 Europe PMC20
  • Book Chapters

    Year Citation
    2019 Srirattana, K., & St John, J. (2019). Transmission of Dysfunctional Mitochondrial DNA and Its Implications for Mammalian Reproduction. In P. Sutovsky (Ed.), Advances in Anatomy Embryology and Cell Biology (Vol. 231, pp. 75-103). Switzerland: Springer Nature.
    DOI Scopus1
    2016 Cagnone, G., Vaghjiani, V., Lee, W., Sun, C., Johnson, J., Yeung, K., & St John, J. (2016). Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. In K. Turksen, & A. Nagy (Eds.), Induced Pluripotent Stem (iPS) Cells: Methods and Protocols (Vol. 1357, pp. 231-267). New York; USA: Springer.
    DOI Scopus1 Europe PMC1
    2016 Sun, X., Lee, W., Vaghjiani, V., & St John, J. (2016). Analysis of mitochondrial DNA copy number and its regulation through DNA methylation of POLGA. In M. McKenzie (Ed.), Mitochondrial DNA: Methods and Protocols (Vol. 1351, pp. 131-141). New York; USA: Springer.
    DOI Scopus1 Europe PMC1
    2015 Lee, W., Kelly, R., Yeung, K., Cagnone, G., McKenzie, M., & St John, J. (2015). Analysis of mitochondrial DNA in induced pluripotent and embryonic stem cells. In P. J. Verma, & H. Sumer (Eds.), Cell Reprogramming: Methods and Protocols (Vol. 1330, pp. 219-252). New York; USA: Springer.
    DOI Scopus1
    2013 Yeung, K., Dickinson, A., & St John, J. (2013). The Role of Mitochondrial DNA in Tumorigenesis. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 119-155).
    DOI
    2013 Facucho-Oliveira, J., Kulkarni, T., Machado-Oliveira, G., & St John, J. (2013). From Pluripotency to Differentiation: The Role of mtDNA in Stem Cell Models of Mitochondrial Diseases. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 87-118).
    DOI
    2013 St John, J. (2013). Mitochondrial DNA: Its Transmission from Gametes and Embryos. In Principles of Cloning: Second Edition (pp. 429-439).
    DOI Scopus1
    2013 Kelly, R., Mahmud, A., & St John, J. (2013). Assisted Reproductive Technologies: The Potential to Prevent the Transmission of Mutant mtDNA from One Generation to the Next. In Mitochondrial DNA, Mitochondria, Disease and Stem Cells (pp. 157-183).
    DOI
    2012 St John, J., & Campbell, K. (2012). The consequences of reprogramming a somatic cell for mitochondrial DNA transmission, inheritance and replication. In Nuclear Reprogramming and Stem Cells (pp. 83-97).
    DOI
  • Conference Papers

    Year Citation
    2009 St John, J. (2009). mtDNA and sperm function. In Systems Biology in Reproductive Medicine Vol. 55 (pp. 17-18).
    2008 Fulka, H., St John, J., Fulka, J., & Hozák, P. (2008). Chromatin in early mammalian embryos: Achieving the pluripotent state. In Differentiation Vol. 76 (pp. 3-14). England.
    DOI Scopus45 Europe PMC27
    2002 St John, J. (2002). The transmission of mitochondrial DNA following assisted reproductive techniques. In Theriogenology Vol. 57 (pp. 109-123). United States.
    DOI Scopus38 Europe PMC21
    1998 Barratt, C., & St John, J. (1998). Diagnostic tools in male infertility. In Human Reproduction Vol. 13 (pp. 51-61). England.
    DOI Scopus13 Europe PMC3
  • Conference Items

    Year Citation
    2014 Chang, J., Chang, C., Chao, S., Cheng, A., Dai, K., Lee, J., . . . Yang, C. (2014). Preface. Poster session presented at the meeting of Geotechnical Special Publication.

2019 – 2022: NHMRC Project Grant (GNT1160106), Understanding how mitochondrial DNA contributes to embryo development (St. John J) $669,790

2018 – 2020: NHMRC Development Grant (GNT1136065), Improving oocyte mitochondrial DNA copy number to enhance female reproductive capacity (St. John J, McKenzie W, Western P, Rombauts L) $670,867.20.

2017 – 2018: The Winston Foundation (WF1), Developing a canine in vitro maturation culture system (St. John J) $42,000

2016 – 2018: OvaScience Inc. Waltham, USA. Renewal Grant, Safety of Mitochondrial Transfer (St. John J) $600,000.

2016 – 2019: ARC Discovery Grant(DP160102575), Diet influences the selective advantage of mitochondrial DNA mutations (Ballard B, St. John J, Smyth G) $313,600.00

2015 – 2017: NHMRC Project Grant(GNT1079309) - Metabolic and molecular basis of embryo signalling (Lane M, St. John J, Zander-Fox D) $397,077.00

2014 – 2016: Australian Mitochondrial Disease Foundation- Preventing the transmission of mutant mitochondrial DNA (St. John J) $25,000

2013 – 2016: OvaScience Inc- Determining the safety of homologous mitochondrial transfer in a mini-pig model (St. John J) $1,250,000

2013 – 2015: NHMRC Project Grant(GNT1041471) - Understanding mitochondrial DNA segregation and transmission (CIA - St. John J; Trounce I) $492,863

2013 – 2015: Zwart Estate- The role of mtDNA variants in glioblastoma (St. John J) $133,131

2013 – 2016: Australian Pork Limited- Defining the role of mitochondrial DNA in pig fertility (St. John J) $92,637

2013 – 2014: Johnston Estate- The generation of stem cell models of mtDNA disease (St. John J) $170,000

2012 – 2014: NHMRC Project Grant(GNT1022222) - Understanding the pathogenesis of mitochondrial disease using iPS cells (Thorburn D, St. John J, Frazier A, Nisbet D, White S) $618,675

2011: Marian and EH Flack Trust- Funding to purchase a High Resolution Melting Curve Analysis Machine(White S, Western P, St. John J, Watkins DN) $30,000.

  • Position: Professor
  • Email: jus.stjohn@adelaide.edu.au
  • Campus: North Terrace
  • Building: Adelaide Health and Medical Sciences, floor 5
  • Org Unit: Paediatrics and Reproductive Health

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