Dr John Bruning

Senior Lecturer

School of Biological Sciences

Faculty of Sciences, Engineering and Technology

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

My Research
Career
Publications
Grants and Funding
Teaching
Supervision
Contact

I head the Laboratory of Protein Crystallography which studeis Nuclear Receptors, Microbial Enzymology, and Broad-Based Cancer Therapeutics. Understanding human disease requires knowledge of the structure-function relationships in protein sensor modulation.This knowledge is incomplete on a broad spectrum of biological systems including nuclear receptor (NR) signalling, proteins involved in cancer biology, and pathogen protein machinery. Our research is focused on understanding these classes of protein interactions on an atomic level. In addition to gaining a basic understanding of protein mechanism, we also focus on structure guided drug design. In our work in understanding NR signalling, we have uncovered new paradigms in NR allostery. These allosteric modulations can now be further studied on a broader scale and harnessed to employ structure guided drug design. Work in the tuberculosis (TB) structural biology consortium has led to a quantum leap in understanding enzymology of the pathogen allowing for new chemotherapy development. We wish to use similar methodology to study a fungal pathogen for the discovery of new anti-fungal molecules.Our research with proteins of the human replication fork, such as PCNA (Proliferating Cellular Nuclear Antigen) has uncovered atomic determinants of these protein modulators that can be used as new paradigms for cancer therapeutic targeting. We will continue this research to aid in the discovery of alternative cancer therapeutic targets and seek to understand their structure-function relationship.

Appointments

Date	Position	Institution name
2016 - ongoing	Adjunct Professor	The Scripps Research Institute
2016 - ongoing	Senior Lecturer Level C (Tenured)	The University of Adelaide
2012 - 2015	Lecturer Level B	The University of Adelaide
2007 - 2012	Associate Research Scientist	Texas A&M University
2005 - 2007	Post-doctoral Research Associate	Scripps Research Institute

Education

Date Institution name Country Title
1998 - 2004 Rice University United States PhD
1994 - 1997 Texas A&M University USA BSc
Postgraduate Training

Date Title Institution Country
2007 - 2012 Assistant Research Scientist Texas A&M USA
2005 - 2007 Post-doctoral Research Assistant The Scripps Research Institute USA

Date	Institution name	Country	Title
1998 - 2004	Rice University	United States	PhD
1994 - 1997	Texas A&M University	USA	BSc

Date	Title	Institution	Country
2007 - 2012	Assistant Research Scientist	Texas A&M	USA
2005 - 2007	Post-doctoral Research Assistant	The Scripps Research Institute	USA

Journals

Year	Citation
2022	Button, E. L., Rossi, J. J., McDougal, D. P., Bruning, J. B., Peet, D. J., Bersten, D. C., . . . Whitelaw, M. L. (2022). Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes. Biochemical Journal, 479(13), 1441-1454. DOI
2022	Downes, C. E., McClure, B. J., McDougal, D. P., Heatley, S. L., Bruning, J. B., Thomas, D., . . . White, D. L. (2022). JAK2 Alterations in Acute Lymphoblastic Leukemia: Molecular Insights for Superior Precision Medicine Strategies. Frontiers in Cell and Developmental Biology, 10, 942053-1-942053-34. DOI Scopus1 WoS1
2022	Ghith, A., Doherty, D. Z., Bruning, J. B., Russell, R. A., De Voss, J. J., & Bell, S. G. (2022). The Structures of the Steroid Binding CYP142 Cytochrome P450 Enzymes from Mycobacterium ulcerans and Mycobacterium marinum. ACS Infectious Diseases, 8(8), 1606-1617. DOI
2022	Pederick, J. L., Horsfall, A. J., Jovcevski‬, B., Klose, J., Abell, A. D., Pukala, T. L., & Bruning, J. B. (2022). Discovery of an ʟ-amino acid ligase implicated in Staphylococcal sulfur amino acid metabolism. Journal of Biological Chemistry, 298(10), 16 pages. DOI
2022	Coleman, T., Lee, J., Kirk, A. M., Doherty, D. Z., Podgorski, M. N., Pinidiya, D. K., . . . Bell, S. G. (2022). Enabling Aromatic Hydroxylation in a Cytochrome P450 Monooxygenase Enzyme through Protein Engineering. Chemistry: A European Journal, 28(67), e202201895-1-e202201895-13. DOI
2022	Podgorski, M. N., Coleman, T., Churchman, L. R., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2022). Investigating the active oxidants involved in cytochrome P450 catalyzed sulfoxidation reactions. Chemistry: A European Journal, 28(72), 1-14. DOI
2022	Coleman, T., Doherty, D. Z., Zhang, T., Podgorski, M. N., Qiao, R., Lee, J. H. Z., . . . Bell, S. G. (2022). Exploring the Factors which Result in Cytochrome P450 Catalyzed Desaturation Versus Hydroxylation. Chemistry: An Asian Journal, 17(24), 1-15. DOI
2022	Stachura, D. L., Nguyen, S., Polyak, S. W., Jovcevski, B., Bruning, J. B., & Abell, A. D. (2022). A New 1,2,3-Triazole Scaffold with Improved Potency against Staphylococcus aureus Biotin Protein Ligase. ACS Infectious Diseases, 8(12), 2579-2585. DOI
2022	Coleman, T., Kirk, A. M., Lee, J. H. Z., Doherty, D. Z., Bruning, J. B., Krenske, E. H., . . . Bell, S. G. (2022). Different geometric requirements for cytochrome P450-catalyzed aliphatic versus aromatic hydroxylation results in chemoselective oxidation. ACS Catalysis, 12(2), 1258-1267. DOI Scopus5 WoS4
2022	Podgorski, M. N., Harbort, J. S., Lee, J. H. Z., Nguyen, G. T. H., Bruning, J. B., Donald, W. A., . . . Bell, S. G. (2022). An altered heme environment in an engineered cytochrome P450 enzyme enables the switch from monooxygenase to peroxygenase activity. ACS Catalysis, 12(3), 1614-1625. DOI Scopus9 WoS8
2022	Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2022). Structural insights into the antifungal drug target guanosine monophosphate synthase from Aspergillus fumigatus. Acta Crystallographica Section D: Structural Biology, 78(Pt 2), 248-259. DOI Scopus1 WoS1 Europe PMC1
2022	Bersten, D. C., Sullivan, A. E., McDougal, D., Breen, J., Fitzsimmons, R. L., Muscat, G. E. O., . . . Whitelaw, M. L. (2022). Core and Flanking bHLH-PAS:DNA interactions mediate specificity and drive obesity. DOI
2022	Nguyen, S., Jovcevski, B., Truong, J. Q., Pukala, T. L., & Bruning, J. B. (2022). A structural model of the human plasminogen and Aspergillus fumigatus enolase complex. Proteins: Structure, Function and Bioinformatics, 90(8), 1509-1520. DOI
2022	Mohamed, H., Child, S. A., Bruning, J. B., & Bell, S. G. (2022). A comparison of the bacterial CYP51 cytochrome P450 enzymes from Mycobacterium marinum and Mycobacterium tuberculosis. The Journal of Steroid Biochemistry and Molecular Biology, 221, 106097-1-106097-14. DOI Scopus1 WoS1
2021	Pederick, J. L., & Bruning, J. B. (2021). An antimony-phosphomolybdate microassay of ATPase activity through the detection of inorganic phosphate. Analytical Biochemistry, 623, 114170. DOI Scopus1 WoS2 Europe PMC1
2021	Horsfall, A. J., Chav, T., Bruning, J. B., & Abell, A. D. (2021). A turn-on fluorescent PCNA sensor. Bioorganic & Medicinal Chemistry Letters, 41, 128031-1-128031-6. DOI
2021	Xie, J., De Poi, S. P., Humphrey, S. J., Hein, L. K., Bruning, J. B., Pan, W., . . . Proud, C. G. (2021). TSC-insensitive Rheb mutations induce oncogenic transformation through a combination of constitutively active mTORC1 signalling and proteome remodelling. Cellular and Molecular Life Sciences, 78(8), 4035-4052. DOI Scopus1 WoS1 Europe PMC1
2021	Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid. Acta Crystallographica Section F Structural Biology Communications, 77(5), 156-162. DOI Scopus1 WoS1 Europe PMC1
2021	Tavakoli Shirazi, P., Eadie, L. N., Page, E. C., Heatley, S. L., Bruning, J. B., & White, D. L. (2021). Constitutive JAK/STAT signaling is the primary mechanism of resistance to JAKi in TYK2-rearranged acute lymphoblastic leukemia. Cancer Letters, 512, 28-37. DOI Scopus4 WoS4 Europe PMC3
2021	Horsfall, A. J., Vandborg, B. A., Kowalczyk, W., Chav, T., Scanlon, D. B., Abell, A. D., & Bruning, J. B. (2021). Unlocking the PIP-box: A peptide library reveals interactions that drive high affinity binding to human PCNA.. The Journal of biological chemistry, 296, 1-16. DOI Scopus2 WoS2 Europe PMC1
2021	Horsfall, A. J., McDougal, D. P., Scanlon, D. B., Bruning, J. B., & Abell, A. D. (2021). Approaches to introduce helical structure in cysteine-containing peptides with a bimane group. Chembiochem, 22(17), 2711-2720. DOI Scopus2 WoS2 Europe PMC1
2021	Qu, Y., Zhang, B., Wang, Y., Yin, S., Pederick, J. L., Bruning, J. B., . . . Bi, J. (2021). Immunogenicity study of engineered ferritins with C- and N-terminus insertion of Epstein-Barr nuclear antigen 1 epitope. Vaccine, 39(34), 4830-4841. DOI Scopus4 WoS2 Europe PMC1
2021	Downes, C. E. J., McClure, B. J., Bruning, J. B., Page, E., Breen, J., Rehn, J., . . . White, D. L. (2021). Acquired JAK2 mutations confer resistance to JAK inhibitors in cell models of acute lymphoblastic leukemia. NPJ Precision Oncology, 5(1), 1-13. DOI Scopus6 WoS4 Europe PMC4
2021	Frkic, R. L., Richter, K., & Bruning, J. B. (2021). The therapeutic potential of inhibiting PPARγ phosphorylation to treat type 2 diabetes.. The Journal of biological chemistry, 297(3), 101030. DOI Scopus11 WoS9 Europe PMC5
2021	Marshall, A. C., & Bruning, J. B. (2021). Engineering potassium activation into biosynthetic thiolase. The Biochemical journal, 478(15), 3047-3062. DOI
2021	Chao, R., Lau, I., Coleman, T., Churchman, L. R., Child, S. A., Lee, J. H. Z., . . . Bell, S. G. (2021). The stereoselective oxidation of para-substituted benzenes by a cytochrome P450 biocatalyst. Chemistry: A European Journal, 27(59), 14765-14777. DOI Scopus3 WoS3
2021	Horsfall, A. J., Vandborg, B., Kikhtyak, Z., Scanlon, D. B., Tilley, W. D., Hickey, T. E., . . . Abell, A. D. (2021). A cell permeable bimane-constrained PCNA-interacting peptide. RSC Chemical Biology, 2(5), 1499-1508. DOI Scopus1
2021	Schumann, N. C., Lee, K. J., Thompson, A. P., Salaemae, W., Pederick, J. L., Avery, T., . . . Abell, A. D. (2021). Inhibition of Mycobacterium tuberculosis dethiobiotin synthase (MtDTBS): toward next-generation antituberculosis agents. ACS Chemical Biology, 16(11), 2339-2347. DOI Scopus2 WoS1 Europe PMC2
2021	Podgorski, M. N., Coleman, T., Giang, P. D., Wang, C. R., Bruning, J. B., Bernhardt, P. V., . . . Bell, S. G. (2021). To be, or not to be, an Inhibitor: a comparison of azole interactions with and oxidation by a cytochrome P450 enzyme. Inorganic Chemistry, 61(1), 236-245. DOI Scopus1 WoS1
2021	Slynko, I., Nguyen, S., Hamilton, E. M. C., Wisse, L. E., de Esch, I. J. P., de Graaf, C., . . . van der Knaap, M. S. (2021). Vanishing white matter: Eukaryotic initiation factor 2B model and the impact of missense mutations.. Molecular genetics & genomic medicine, 9(3), 1-15. DOI Scopus8 WoS9 Europe PMC5
2021	Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2021). Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase.. The FEBS journal, 288(7), 2398-2417. DOI Scopus5 WoS5 Europe PMC3
2021	Nguyen, S., Truong, J. Q., & Bruning, J. B. (2021). Targeting Unconventional Pathways in Pursuit of Novel Antifungals.. Front Mol Biosci, 7, 1-16. DOI Scopus8 WoS6 Europe PMC3
2021	Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Derivatization of protein crystals with I3C using random microseed matrix screening. Journal of Visualized Experiments, 2021(167), 1-19. DOI WoS1
2021	Coleman, T., Kirk, A. M., Chao, R. R., Podgorski, M. N., Harbort, J. S., Churchman, L. R., . . . Bell, S. G. (2021). Understanding the Mechanistic Requirements for Efficient and Stereoselective Alkene Epoxidation by a Cytochrome P450 Enzyme. ACS Catalysis, 11(4), 1995-2010. DOI Scopus15 WoS15
2020	Jia, Y., Shen, G., Nguyen, S., Zhang, Y., Huang, K. -S., Ho, H. -Y., . . . Wang, W. -L. (2020). Analysis of the mutation dynamics of SARS-CoV-2 reveals the spread history and emergence of RBD mutant with lower ACE2 binding affinity. DOI Europe PMC2
2020	Horsfall, A. J., Abell, A. D., & Bruning, J. (2020). Targeting PCNA with peptide mimetics for therapeutic purposes. ChemBioChem, 21(4), 442-450. DOI Scopus14 WoS13 Europe PMC7
2020	Podgorski, M. N., Coleman, T., Chao, R. R., De Voss, J. J., Bruning, J. B., & Bell, S. G. (2020). Investigation of the requirements for efficient and selective cytochrome P450 monooxygenase catalysis across different reactions. Journal of Inorganic Biochemistry, 203, 110913-1-110913-13. DOI Scopus14 WoS14 Europe PMC3
2020	Podgorski, M. N., Harbort, J. S., Coleman, T., Stok, J. E., Yorke, J. A., Wong, L. -L., . . . Bell, S. G. (2020). Biophysical techniques for distinguishing ligand binding modes in cytochrome P450 monooxygenases. Biochemistry, 59(9), 1038-1050. DOI Scopus8 WoS9 Europe PMC2
2020	Coleman, T., Stok, J. E., Podgorski, M. N., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2020). Structural insights into the role of the acid-alcohol pair of residues required for dioxygen activation in cytochrome P450 enzymes. JBIC Journal of Biological Inorganic Chemistry, 25(4), 583-596. DOI Scopus13 WoS15 Europe PMC1
2020	Pederick, J. L., Thompson, A. P., Bell, S. G., & Bruning, J. B. (2020). D-alanine-D-alanine ligase as a model for the activation of ATP-grasp enzymes by monovalent cations.. The Journal of biological chemistry, 295(23), 7894-7904. DOI Scopus9 WoS8 Europe PMC5
2020	Child, S. A., Ghith, A., Bruning, J. B., & Bell, S. G. (2020). A comparison of steroid and lipid binding cytochrome P450s from Mycobacterium marinum and Mycobacterium tuberculosis. Journal of Inorganic Biochemistry, 209, 1-12. DOI Scopus4 WoS4 Europe PMC1
2020	Xie, J., De Poi, S., Humphrey, S., Hein, L., Bruning, J., Pan, W., . . . Proud, C. (2020). TSC-Insensitive Rheb Mutations Induce Oncogenic Transformation Through a Combination of Hyperactive mTORC1 Signalling and Metabolic Reprogramming. DOI
2019	Truong, J. Q., Panjikar, S., Shearwin-Whyatt, L., Bruning, J. B., & Shearwin, K. E. (2019). Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68. Acta crystallographica Section D. Structural biology, 75(7), 670-681. DOI Scopus3 WoS2 Europe PMC1
2019	Lee, K. J., Tieu, W., Blanco-Rodriguez, B., Paparella, A. S., Yu, J., Hayes, A., . . . Abell, A. D. (2019). Sulfonamide-based inhibitors of biotin protein ligase as new antibiotic leads. ACS Chemical Biology, 14(9), 1990-1997. DOI Scopus4 WoS4 Europe PMC1
2019	Jia, Y., Burbidge, C. A., Sweetman, C., Schutz, E., Soole, K., Jenkins, C., . . . Ford, C. M. (2019). An aldo-keto reductase with 2-keto- L-gulonate reductase activity functions in L-tartaric acid biosynthesis from vitamin C in Vitis vinifera. The Journal of biological chemistry, 294(44), 15932-15946. DOI Scopus12 WoS10 Europe PMC5
2019	Schumann, N., Bruning, J., Marshall, A., & Abell, A. (2019). The role of N-terminal heterocycles in hydrogen bonding to α-chymotrypsin. Bioorganic and Medicinal Chemistry Letters, 29(3), 396-399. DOI Scopus2 WoS2 Europe PMC1
2019	Marshall, A. C., Kidd, S. E., Lamont-Friedrich, S. J., Arentz, G., Hoffmann, P., Coad, B. R., & Bruning, J. B. (2019). Structure, mechanism, and inhibition of aspergillus fumigatus thioredoxin reductase. Antimicrobial Agents and Chemotherapy, 63(3), 1-15. DOI Scopus19 WoS19 Europe PMC8
2019	Elzahhar, P., Alaaeddine, R., Ibrahim, T., Nassra, R., Ismail, A., Chua, B., . . . Belal, A. (2019). Shooting three inflammatory targets with a single bullet: Novel multi-targeting anti-inflammatory glitazones. European Journal of Medicinal Chemistry, 167, 562-582. DOI Scopus19 WoS19 Europe PMC6
2019	Child, S., Flint, K., Bruning, J., & Bell, S. (2019). The characterisation of two members of the cytochrome P450 CYP150 family: CYP150A5 and CYP150A6 from Mycobacterium marinum. Biochimica et Biophysica Acta - General Subjects, 1863(5), 925-934. DOI Scopus4 WoS4
2019	Chang, M. R., Ciesla, A., Strutzenberg, T. S., Novick, S. J., He, Y., Garcia-Ordonez, R. D., . . . Griffin, P. R. (2019). Unique Polypharmacology Nuclear Receptor Modulator Blocks Inflammatory Signaling Pathways. ACS Chemical Biology, 14(5), 1051-1062. DOI Scopus7 WoS7 Europe PMC3
2018	Marshall, A., Keiller, B., Pederick, J., Abell, A., & Bruning, J. (2018). Crystal structure of bovine alpha-chymotrypsin in space group P65. Crystals, 8(12), 460. DOI Scopus1 WoS1
2018	Frkic, R. L., Chua, B. S., Shin, Y., Pascal, B. D., Novick, S. J., Kamenecka, T. M., . . . Bruning, J. B. (2018). Structural and dynamic elucidation of a non-acid PPARγ partial agonist: SR1988. Nuclear Receptor Research, 5, 101350-1-101350-12. DOI Europe PMC3
2018	Child, S., Naumann, E., Bruning, J., & Bell, S. (2018). Structural and functional characterisation of the cytochrome P450 enzyme CYP268A2 from Mycobacterium marinum. The Biochemical Journal, 475(4), 705-722. DOI Scopus9 WoS9 Europe PMC2
2018	Marshall, A., Bond, C., & Bruning, J. (2018). Structure of Aspergillus fumigatus Cytosolic thiolase: trapped tetrahedral reaction intermediates and activation by monovalent cations. ACS Catalysis, 8(3), 1973-1989. DOI Scopus3 WoS3
2018	Coleman, T., Wong, S., Podgorski, M., Bruning, J., De Voss, J., & Bell, S. (2018). Cytochrome P450 CYP199A4 from Rhodopseudomonas palustris catalyses heteroatom dealkylations, sulfoxidation and amide and cyclic hemiacetal formation. ACS Catalysis, 8(7), 5915-5927. DOI Scopus19 WoS19
2018	Wegener, K. L., McGrath, A. E., Dixon, N. E., Oakley, A. J., Scanlon, D. B., Abell, A. D., & Bruning, J. (2018). Rational design of a 310-helical PIP-box mimetic targeting PCNA - the human sliding clamp. Chemistry - A European Journal, 24(44), 11325-11331. DOI Scopus9 WoS9 Europe PMC4
2018	Thompson, A., Wegener, K., Booker, G., Polyak, S., & Bruning, J. (2018). Precipitant-ligand exchange technique reveals the ADP binding mode in Mycobacterium tuberculosis dethiobiotin synthetase. Acta Crystallographica Section D: Structural Biology, 74(10), 965-972. DOI Scopus3 WoS3
2018	Frkic, R. L., Marshall, A. C., Blayo, A. -L., Pukala, T. L., Kamenecka, T. M., Griffin, P. R., & Bruning, J. B. (2018). PPARγ in complex with an antagonist and inverse agonist: a tumble and trap mechanism of the activation helix. iScience, 5, 69-79. DOI Scopus23 WoS24 Europe PMC11
2018	Zheng, J., Corzo, C., Chang, M., Shang, J., Lam, V., Brust, R., . . . Griffin, P. (2018). Chemical crosslinking mass spectrometry reveals the conformational landscape of the activation helix of PPARγ; a model for ligand-dependent antagonism. Structure, 26(11), 1431-1439. DOI Scopus15 WoS15 Europe PMC9
2018	Thompson, A. P., Salaemae, W., Pederick, J. L., Abell, A. D., Booker, G. W., Bruning, J. B., . . . Polyak, S. W. (2018). Mycobacterium tuberculosis Dethiobiotin Synthetase Facilitates Nucleoside Triphosphate Promiscuity through Alternate Binding Modes. ACS Catalysis, 8(11), 10774-10783. DOI Scopus6 WoS5
2017	Stevens, T., Song, S., Bruning, J., Choo, A., & Baxter, S. (2017). Expressing a moth abcc2 gene in transgenic Drosophila causes susceptibility to Bt Cry1Ac without requiring a cadherin-like protein receptor. Insect Biochemistry and Molecular Biology, 80, 61-70. DOI Scopus37 WoS35 Europe PMC15
2017	Marshall, A., Kroker, A., Murray, L., Gronthos, K., Rajapaksha, H., Wegener, K., & Bruning, J. (2017). Structure of the sliding clamp from the fungal pathogen Aspergillus fumigatus (AfumPCNA) and interactions with Human p21. FEBS Journal, 284(6), 985-1002. DOI Scopus8 WoS8 Europe PMC5
2017	Sternicki, L., Wegener, K., Bruning, J., Booker, G., & Polyak, S. (2017). Mechanisms governing precise protein biotinylation. Trends in Biochemical Sciences, 42(5), 383-394. DOI Scopus17 WoS14 Europe PMC6
2017	Rajapaksha, H., Bhatia, H., Wegener, K., Petrovsky, N., & Bruning, J. (2017). X-ray crystal structure of rivoglitazone bound to PPARγ and PPAR subtype selectivity of TZDs. Biochimica et Biophysica Acta - General Subjects, 1861(8), 1981-1991. DOI Scopus12 WoS13 Europe PMC1
2017	Frkic, R., He, Y., Rodriguez, B., Chang, M., Kuruvilla, D., Ciesla, A., . . . Bruning, J. (2017). Structure-Activity Relationship of 2,4-Dichloro-N-(3,5-dichloro-4-(quinolin-3-yloxy)phenyl)benzenesulfonamide (INT131) Analogs for PPARγ-Targeted Antidiabetics. Journal of Medicinal Chemistry, 60(11), 4584-4593. DOI Scopus19 WoS16 Europe PMC7
2017	Zheng, J., Chang, M., Stites, R., Wang, Y., Bruning, J., Pascal, B., . . . Griffin, P. (2017). HDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions. Nature Communications, 8(1), 923-1-923-13. DOI Scopus24 WoS24 Europe PMC12
2016	Van Marrewijk, L., Polyak, S., Hijnen, M., Kuruvilla, D., Chang, M., Shin, Y., . . . Bruning, J. (2016). SR2067 reveals a unique kinetic and structural signature for PPARγ partial agonism. ACS Chemical Biology, 11(1), 273-283. DOI Scopus27 WoS24 Europe PMC13
2016	Zhang, X., Bruning, J., George, J., & Abell, A. (2016). A mechanistic study on the inhibition of α-chymotrypsin by a macrocyclic peptidomimetic aldehyde. Organic and Biomolecular Chemistry, 14(29), 6970-6978. DOI Scopus9 WoS9
2016	Stechschulte, L., Czernik, P., Rotter, Z., Tausif, F., Corzo, C., Marciano, D., . . . Lecka-Czernik, B. (2016). PPARG Post-translational modifications regulate bone formation and bone resorption. EBioMedicine, 10, 174-184. DOI Scopus49 WoS48 Europe PMC36
2015	Jia, Y., Wong, D., Sweetman, C., Bruning, J., & Ford, C. (2015). New insights into the evolutionary history of plant sorbitol dehydrogenase. BMC Plant Biology, 15(1), 101-1-101-23. DOI Scopus17 WoS18 Europe PMC10
2015	Kroker, A., & Bruning, J. (2015). p21 exploits residue Tyr151 as a tether for high-affinity PCNA binding. Biochemistry, 54(22), 3483-3493. DOI Scopus22 WoS21 Europe PMC15
2015	Coleman, T., Chao, R., Bruning, J., De Voss, J., & Bell, S. (2015). CYP199A4 catalyses the efficient demethylation and demethenylation of para-substituted benzoic acid derivatives. RSC Advances, 5(64), 52007-52018. DOI Scopus21 WoS21
2015	Marciano, D., Kuruvilla, D., Boregowda, S., Asteian, A., Hughes, T., Garcia-Ordonez, R., . . . Griffin, P. (2015). Pharmacological repression of PPARγ promotes osteogenesis. Nature Communications, 6(1), 7443-1-7443-7. DOI Scopus81 WoS78 Europe PMC55
2015	Kojetin, D., Matta-Camacho, E., Hughes, T., Srinivasan, S., Nwachukwu, J., Cavett, V., . . . Nettles, K. (2015). Structural mechanism for signal transduction in RXR nuclear receptor heterodimers. Nature Communications, 6(1), 8013-1-8013-14. DOI Scopus88 WoS83 Europe PMC56
2015	Kroker, A., & Bruning, J. (2015). Review of the structural and dynamic mechanisms of PPAR γ partial agonism. PPAR Research, 2015, 816856-1-816856-15. DOI Scopus114 WoS103 Europe PMC64
2014	Chua, K., Pietsch, M., Zhang, X., Hautmann, S., Chan, H., Bruning, J., . . . Abell, A. (2014). Macrocyclic protease inhibitors with reduced peptide character. Angewandte Chemie International Edition, 53(30), 7828-7831. DOI Scopus21 WoS20 Europe PMC7
2014	Sullivan, A., Raimondo, A., Schwab, T., Bruning, J., Froguel, P., Farooqi, I., . . . Whitelaw, M. (2014). Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2. Biochemical Journal, 461(3), 403-412. DOI Scopus9 WoS8 Europe PMC8
2014	Bersten, D., Bruning, J., Peet, D., & Whitelaw, M. (2014). Human variants in the Neuronal Basic Helix-Loop-Helix/ Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. PLoS One, 9(1), e85768-1-e85768-11. DOI Scopus19 WoS19 Europe PMC19
2014	Reddy, M., Breda, A., Bruning, J., Sherekar, M., Valluru, S., Thurman, C., . . . Sacchettini, J. (2014). Structure, activity, and inhibition of the carboxyltransferase β-Subunit of acetyl coenzyme a carboxylase (AccD6) from mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 58(10), 6122-6132. DOI Scopus16 WoS14 Europe PMC7
2013	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2013). Redefining the role of the quaternary shift in bacillus stearothermophilus phosphofructokinase. Biochemistry, 52(32), 5421-5429. DOI Scopus4 WoS4 Europe PMC2
2013	Bonnefond, A., Raimondo, A., Stutzmann, F., Ghoussaini, M., Ramachandrappa, S., Bersten, D., . . . Froguel, P. (2013). Loss-of-function mutations in SIM1 contribute to obesity and Prader-Willi-like features. Journal of Clinical Investigation, 123(7), 3037-3041. DOI Scopus81 WoS73 Europe PMC58
2013	Ramachandrappa, S., Raimondo, A., Cali, A., Keogh, J., Henning, E., Saeed, S., . . . Farooqi, I. (2013). Rare variants in single-minded 1 (SIM1) are associated with severe obesity. Journal of Clinical Investigation, 123(7), 3042-3050. DOI Scopus109 WoS95 Europe PMC78
2012	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2012). Structure of the apo form of Bacillus Stearothermophilus Phosphofructokinase. Biochemistry, 51(3), 769-775. DOI Scopus8 WoS8 Europe PMC6
2011	Bruning, J., Murillo, A., Chacon, O., Barletta, R., & Sacchettini, J. (2011). Structure of the Mycobacterium tuberculosis D-alanine:D-alanine Ligase, a target of the antituberculosis drug D-Cycloserine. Antimicrobial Agents and Chemotherapy, 55(1), 291-301. DOI Scopus100 WoS95 Europe PMC54
2011	Zhang, J., Chalmers, M., Stayrook, K., Burris, L., Wang, Y., Busby, S., . . . Griffin, P. (2011). DNA binding alters coactivator interaction surfaces of the intact VDR-RXR complex. Nature Structural and Molecular Biology, 18(5), 556-563. DOI Scopus155 WoS143 Europe PMC108
2011	Chim, N., Habel, J., Johnston, J., Krieger, I., Miallau, L., Sankaranarayanan, R., . . . Goulding, C. (2011). The TB structural genomics consortium: a decade of progress. Tuberculosis, 91(2), 155-172. DOI Scopus34 WoS32 Europe PMC24
2011	Choi, J., Banks, A., Kamenecka, T., Busby, S., Chalmers, M., Kumar, N., . . . Griffin, P. (2011). Anti-diabetic actions of a non-agonist PPARgamma ligand that blocks Cdk5-mediated phosphorylation. Nature, 477(7365), 477-481. DOI Scopus411 WoS378 Europe PMC297
2010	Bruning, J., Parent, A., Gil, G., Zhao, M., Nowak, J., Pace, M., . . . Nettles, K. (2010). Coupling of receptor conformation and ligand orientation determine graded activity. Nature Chemical Biology, 6(11), 837-843. DOI Scopus107 WoS103 Europe PMC75
2009	Reddy, M., Palaninathan, S., Bruning, J., Thurman, C., Smith, D., & Sacchettini, J. (2009). Structural insights into the mechanism of the allosteric transitions of Mycobacterium Tuberculosis cAMP receptor protein. Journal of Biological Chemistry, 284(52), 36581-36591. DOI Scopus31 WoS28 Europe PMC19
2008	Nettles, K., Bruning, J., Gil, G., Nowak, J., Sharma, S., Hahm, J., . . . Greene, G. (2008). NFκB selectivity of estrogen receptor ligands revealed by comparative crystallographic analyses. Nature Chemical Biology, 4(4), 241-247. DOI Scopus127 WoS124 Europe PMC83
2008	Dai, S., Chalmers, M., Bruning, J., Bramlett, K., Osborne, H., Montrose-Rafizadeh, C., . . . Griffin, P. (2008). Prediction of the tissue-specificity of selective estrogen receptor modulators by using a single biochemical method. Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7171-7176. DOI Scopus73 WoS66 Europe PMC52
2007	Nettles, K., Bruning, J., Gil, G., O'Neill, E., Nowak, J., Hughs, A., . . . Greene, G. (2007). Structural plasticity in the oestrogen receptor ligand-binding domain. EMBO Reports, 8(6), 563-568. DOI Scopus109 WoS106 Europe PMC79
2007	Zhou, H., Nettles, K., Bruning, J., Kim, Y., Joachimiak, A., Sharma, S., . . . Katzenellenbogen, J. (2007). Elemental isomerism: a boron-nitrogen surrogate for a carbon-carbon double bond increases the chemical diversity of estrogen receptor ligands. Chemistry & Biology, 14(6), 659-669. DOI Scopus62 WoS62 Europe PMC38
2007	Bruning, J., Chalmers, M., Prasad, S., Busby, S., Kamenecka, T., He, Y., . . . Griffin, P. (2007). Partial agonists activate PPARγ using a helix 12 independent mechanism. Structure, 15(10), 1258-1271. DOI Scopus272 WoS261 Europe PMC171
2004	Bruning, J. B., & Shamoo, Y. (2004). Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-δ p66 subunit and flap endonuclease-1. Structure, 12(12), 2209-2219. DOI Scopus163 WoS163 Europe PMC131

Book Chapters

Year	Citation
2021	Chua, B. S. K., & Bruning, J. B. (2021). PPARα and δ Ligand Design: Honing the Traditional Empirical Method with a More Holistic Overview. In M. Z. Badr (Ed.), Nuclear Receptors: The Art and Science of Modulator Design and Discovery (pp. 111-178). Cham, Switzerland: Springer. DOI
2019	Frkic, R., & Bruning, J. (2019). Obtaining crystals of PPARγ ligand binding domain bound to small molecules. In Unknown Book (Vol. 1966, pp. 253-260). DOI Scopus1 Europe PMC1

Conference Papers

Year	Citation
2010	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J. C., & Reinhart, G. D. (2010). Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase. In BIOPHYSICAL JOURNAL Vol. 98 (pp. 39A). CELL PRESS. DOI
2007	Chalmers, M. J., Busby, S. A., Prasad, S., Bruning, J. B., Pascal, B. D., Southern, M. R., & Griffin, P. R. (2007). P97-S A High-Throughput Method for the characterization of Nuclear Receptor Dynamics. In J Biomol Tech. Vol. 18 (pp. 33). Tampa Florida.

Conference Items

Year	Citation
2020	Tavakoli, P., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent Activation of JAK/STAT Signaling Plays an Important Role inin VitroJaki Resistance in TYK2-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: AMER SOC HEMATOLOGY. DOI
2020	Tavakoli, P. S., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent activation of JAK/STAT signaling plays an important role in in vitro Jaki resistance in TYK2-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of 62nd Annual Meeting of the American Society of Hematology. San Diego, California (virtual conference).
2020	Downes, C. E. J., McClure, B. J., Rehn, J., Breen, J., Bruning, J. B., Yeung, D. T., & White, D. L. (2020). Acquired Mutations within the JAK2 Kinase Domain Confer Resistance to JAK Inhibitors in an in Vitro model of a High-Risk Acute Lymphoblastic Leukemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: AMER SOC HEMATOLOGY. DOI WoS1
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of APG Student Awards.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of ruxolitinib resistance mutations in Pro-B cells driven by a high-risk JAK2 fusion in B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of ASMR.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and Characterisation of Ruxolitinib Resistant Mutations in JAK2-rearranged B-cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of AGTA.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of mutations that cause ruxolitinib resistance in Pro-B cells driven by a high-risk B-ALL JAK2-fusion.. Poster session presented at the meeting of ANZCHOG.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of SAHMRI Scientific Seminar.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of EMBL Australia Postgraduate Symposium.

Original Creative Works

Year	Citation
2018	Authors: Wegener K, Mcgrath A, Dixon N, Oakley A, Scanlon D, Abell A, Bruning J. Title: Rational Design of a 310-Helical PIP-Box Mimetic Targeting PCNA, the Human Sliding Clamp [Cover Profile]. Description: N/A. Extent: N/A. DOI

ARC LIEF Grant (2014). LE15010023. Michael, Dr. Micahel Z; Booker, A/Prof., Grant W; Venter, Dr. Henrietta; Pitson, Prof. Stuart M; McKinnon, Prof. Ross A; Abell, Prof. Andrew D; Soole, A/Prof. Kathleen L; Sykes, Prof. Pamela J; Bruning, Dr. John B. “High Throughput Screening Facility.” ($860,000).
Environment Institute Major Collaborative Project (2015). Dr. Frank Reith, Prof. Peter Hoffmann, Dr. Florian Weiland, Dr. Benjamin Wade, Dr. John Bruning. ($50,000).
Biochemistry Discipline Collaborative Research Grant (2015-2018). Bruning, JB and Booker, GW. “Structural studies of Aspergillus fumigatus enzyme targets for drug discovery.” ($45,000).
AIB Labs, Research Infrastructure Fund (2015). Parkinson-Lawrence, E., Polyak, S.W., Brooks, D., Booker, G.W., Forbes, B.E., Pitson, S., Cowin, A., Bruning, J., Butler, L. ($12,793).
IPAS Pilot Projects (2015). Krogsgaard-Larsen, N.; Bruning, J.B.; Luiten, A.; Callen, D.; Zhang, X.Z.; Alaknanda, A. “First Steps toward a Cancer Biosensor.” ($6,000).
Australian Synchrotron CAP (2013). CAP6919. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”
Australian Synchrotron CAP (2014). CAP8221. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”

BIOCHEM3000 – Molecular and Structural Biology
BIOCHEM3230 – Molecular and Structural Biology (Biomedical Science)
BIOCHEM3125 – Advanced Molecular Biology IIIA (Biochemistry)
BIOCHEM3001 – Cancer, Stem Cells and Development III
BIOCHEM3235 – Cancer, Stem Cells and Development III (Biomedical Science)
BIOCHEM3520 – Cancer, Stem Cells and Development (Theory) III
BIOCHEM3225 – Advanced Molecular Biology IIIB (Biochemistry)
BIOCHEM2500 – Biochemistry II: Molecular & Cell Biology
BIOCHEM2502 – Biochemistry II (Biotech): Molecular & Cell Biology
BIOCHEM2504 – Biochemistry II (Mol Biol): Molecular & Cell Biology
BIOLOGY1201 – Biology I – Human Perspectives
CHEMENG 2015 - Principles of Biotechnology II
BIOTECH7000 – Advanced Research Platforms
BIOTECH7001 – Drug Discovery and Development
Science2300 – Principles and Practice of Research

Current Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2022	Co-Supervisor	Researching the Interaction, Structure and Function of S-Proteins in Almond	Doctor of Philosophy under a Jointly-awarded Degree Agreement with	Doctorate	Full Time	Ms Isis Pinner
2021	Principal Supervisor	Development and Analysis of Gold Sensing Protein	Doctor of Philosophy	Doctorate	Full Time	Mr Akhil Kumar
2020	Principal Supervisor	Peptidomimetic approaches for inhibiting sliding clamp proteins	Doctor of Philosophy	Doctorate	Full Time	Miss Bethiney Chantel Vandborg
2020	Co-Supervisor	Development of 2-aminoquinoline Derivatives with Improved Drug-like Character as Small Molecule Inhibitors for the Tec SH3 Domain	Doctor of Philosophy	Doctorate	Part Time	Ms Ellen Lillian Swan
2019	Principal Supervisor	Structural Mechanisms of Estrogen Receptor Allostery and Endocrine Disruption	Doctor of Philosophy	Doctorate	Full Time	Mr Daniel Patrick McDougal
2018	Principal Supervisor	Functional and Structural Characterisation of Bacterial ATP-grasp Ligases	Doctor of Philosophy	Doctorate	Full Time	Mr Jordan Luke Pederick
2018	Co-Supervisor	Structural and Functional Investigations of Cytochrome P450 Enzymes from Mycobacterium Species	Doctor of Philosophy	Doctorate	Full Time	Miss Hebatalla Ahmed Ibrahim Mohamed

Past Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2019 - 2021	Co-Supervisor	Targeting PCNA with Cell and Nuclear Permeable p21- derived Peptides	Master of Philosophy	Master	Full Time	Miss Theresa Chav
2018 - 2021	Principal Supervisor	Structural and Biochemical Insights into Antifungal Drug Targets from Aspergillus fumigatus	Doctor of Philosophy	Doctorate	Full Time	Ms Stephanie Nguyen
2018 - 2022	Co-Supervisor	Precision Medicine Approaches for JAK2-Rearranged Acute Lymphoblastic Leukaemia: The Efficacy of Targeted Therapies and Molecular Mechanisms of Drug Resistance	Doctor of Philosophy	Doctorate	Full Time	Miss Charlotte Emma-Jeane Downes
2018 - 2021	Co-Supervisor	Approaches to study protein interactions	Doctor of Philosophy	Doctorate	Full Time	Miss Aimee Jade Horsfall
2018 - 2019	Co-Supervisor	Structural and Biophysical Characterisation of Mycobacterium tuberculosis Dethiobiotin Synthetase	Doctor of Philosophy	Doctorate	Full Time	Andrew Philip Thompson
2017 - 2021	Principal Supervisor	Understanding PPARg-Ligand Interactions to Treat Type 2 Diabetes	Doctor of Philosophy	Doctorate	Full Time	Miss Rebecca Louise Frkic
2017 - 2019	Co-Supervisor	Microbial Biosynthesis of Grapevine Specialised Metabolites	Master of Philosophy	Master	Full Time	Miss Emi Kaye Schutz
2016 - 2017	Principal Supervisor	Defining the Key Residues in PCNA-Protein Interactions	Master of Philosophy	Master	Part Time	Alice Jane Kroker
2016 - 2021	Co-Supervisor	Eukaryotic elongation factor 2 kinase (eEF2K): its relation to cancer cell survival, autophagy and other cell signalling pathways	Doctor of Philosophy	Doctorate	Full Time	Mr Roman Valeryevich Lenchine
2015 - 2021	Co-Supervisor	Structural Characterization of Lysogeny-Promoting Transcription Factor of Bacteriophage 186	Doctor of Philosophy	Doctorate	Full Time	Mr Jia Quyen Truong
2014 - 2018	Principal Supervisor	Structural and Biochemical Studies on Three Aspergillus fumigatus Proteins That Present as Targets for Novel Antifungal Drugs	Doctor of Philosophy	Doctorate	Full Time	Andrew Craig Marshall
2014 - 2016	Co-Supervisor	Molecular and Structural Characterization of the Candidate Enzymes Responsible for Tartaric Acid Synthesis in the Grapevine	Doctor of Philosophy	Doctorate	Full Time	Mr Yong Jia

Position: Senior Lecturer
Phone: 83135218
Email: john.bruning@adelaide.edu.au
Fax: 83134362
Campus: North Terrace
Building: Molecular Life Sciences, floor 2
Org Unit: Molecular and Biomedical Science

Dr John Bruning

Appointments

Education

Postgraduate Training