Dr John Bruning
Senior Lecturer
School of Biological Sciences
Faculty of Sciences
Eligible to supervise Masters and PhD, but is currently at capacity - email supervisor to discuss availability.
I head the Laboratory of Protein Crystallography which studeis Nuclear Receptors, Microbial Enzymology, and Broad-Based Cancer Therapeutics. Understanding human disease requires knowledge of the structure-function relationships in protein sensor modulation.This knowledge is incomplete on a broad spectrum of biological systems including nuclear receptor (NR) signalling, proteins involved in cancer biology, and pathogen protein machinery. Our research is focused on understanding these classes of protein interactions on an atomic level. In addition to gaining a basic understanding of protein mechanism, we also focus on structure guided drug design. In our work in understanding NR signalling, we have uncovered new paradigms in NR allostery. These allosteric modulations can now be further studied on a broader scale and harnessed to employ structure guided drug design. Work in the tuberculosis (TB) structural biology consortium has led to a quantum leap in understanding enzymology of the pathogen allowing for new chemotherapy development. We wish to use similar methodology to study a fungal pathogen for the discovery of new anti-fungal molecules.Our research with proteins of the human replication fork, such as PCNA (Proliferating Cellular Nuclear Antigen) has uncovered atomic determinants of these protein modulators that can be used as new paradigms for cancer therapeutic targeting. We will continue this research to aid in the discovery of alternative cancer therapeutic targets and seek to understand their structure-function relationship.
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Appointments
Date Position Institution name 2016 Adjunct Professor The Scripps Research Institute 2016 Senior Lecturer Level C (Tenured) The University of Adelaide 2012 - 2015 Lecturer Level B The University of Adelaide 2007 - 2012 Associate Research Scientist Texas A&M University 2005 - 2007 Post-doctoral Research Associate Scripps Research Institute -
Education
Date Institution name Country Title 1998 - 2004 Rice University United States PhD 1994 - 1997 Texas A&M University USA BSc -
Postgraduate Training
Date Title Institution Country 2007 - 2012 Assistant Research Scientist Texas A&M USA 2005 - 2007 Post-doctoral Research Assistant The Scripps Research Institute USA
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Journals
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Conference Papers
Year Citation 2010 Mosser, R., Reddy, M., Bruning, J., Sacchettini, J. C., & Reinhart, G. D. (2010). Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase. In BIOPHYSICAL JOURNAL Vol. 98 (pp. 39A). CELL PRESS.
2007 Chalmers, M. J., Busby, S. A., Prasad, S., Bruning, J. B., Pascal, B. D., Southern, M. R., & Griffin, P. R. (2007). P97-S A High-Throughput Method for the characterization of Nuclear Receptor Dynamics. In J Biomol Tech. Vol. 18 (pp. 33). Tampa Florida. -
Conference Items
Year Citation 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of APG Student Awards. 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of ruxolitinib resistance mutations in Pro-B cells driven by a high-risk JAK2 fusion in B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of ASMR. 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and Characterisation of Ruxolitinib Resistant Mutations in JAK2-rearranged B-cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of AGTA. 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of mutations that cause ruxolitinib resistance in Pro-B cells driven by a high-risk B-ALL JAK2-fusion.. Poster session presented at the meeting of ANZCHOG. 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of SAHMRI Scientific Seminar. 2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of EMBL Australia Postgraduate Symposium.
- ARC LIEF Grant (2014). LE15010023. Michael, Dr. Micahel Z; Booker, A/Prof., Grant W; Venter, Dr. Henrietta; Pitson, Prof. Stuart M; McKinnon, Prof. Ross A; Abell, Prof. Andrew D; Soole, A/Prof. Kathleen L; Sykes, Prof. Pamela J; Bruning, Dr. John B. “High Throughput Screening Facility.” ($860,000).
- Environment Institute Major Collaborative Project (2015). Dr. Frank Reith, Prof. Peter Hoffmann, Dr. Florian Weiland, Dr. Benjamin Wade, Dr. John Bruning. ($50,000).
- Biochemistry Discipline Collaborative Research Grant (2015-2018). Bruning, JB and Booker, GW. “Structural studies of Aspergillus fumigatus enzyme targets for drug discovery.” ($45,000).
- AIB Labs, Research Infrastructure Fund (2015). Parkinson-Lawrence, E., Polyak, S.W., Brooks, D., Booker, G.W., Forbes, B.E., Pitson, S., Cowin, A., Bruning, J., Butler, L. ($12,793).
- IPAS Pilot Projects (2015). Krogsgaard-Larsen, N.; Bruning, J.B.; Luiten, A.; Callen, D.; Zhang, X.Z.; Alaknanda, A. “First Steps toward a Cancer Biosensor.” ($6,000).
- Australian Synchrotron CAP (2013). CAP6919. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”
- Australian Synchrotron CAP (2014). CAP8221. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”
- BIOCHEM3000 – Molecular and Structural Biology
- BIOCHEM3230 – Molecular and Structural Biology (Biomedical Science)
- BIOCHEM3125 – Advanced Molecular Biology IIIA (Biochemistry)
- BIOCHEM3001 – Cancer, Stem Cells and Development III
- BIOCHEM3235 – Cancer, Stem Cells and Development III (Biomedical Science)
- BIOCHEM3520 – Cancer, Stem Cells and Development (Theory) III
- BIOCHEM3225 – Advanced Molecular Biology IIIB (Biochemistry)
- BIOCHEM2500 – Biochemistry II: Molecular & Cell Biology
- BIOCHEM2502 – Biochemistry II (Biotech): Molecular & Cell Biology
- BIOCHEM2504 – Biochemistry II (Mol Biol): Molecular & Cell Biology
- BIOLOGY1201 – Biology I – Human Perspectives
- CHEMENG 2015 - Principles of Biotechnology II
- BIOTECH7000 – Advanced Research Platforms
- BIOTECH7001 – Drug Discovery and Development
- Science2300 – Principles and Practice of Research
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Current Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2019 Principal Supervisor Structural Mechanisms of Estrogen Receptor Allostery and Endocrine Disruption Doctor of Philosophy Doctorate Full Time Mr Daniel Patrick McDougal 2018 Principal Supervisor Biochemistry Doctor of Philosophy Doctorate Full Time Ms Stephanie Nguyen 2018 Principal Supervisor Biochemistry Doctor of Philosophy Doctorate Full Time Mr Jordan Luke Pederick 2018 Co-Supervisor Characterising signalling cascades and investigating therapeutic targets in JAK2-rearranged acute lymphoblastic leukaemia Doctor of Philosophy Doctorate Full Time Miss Charlotte Emma-Jeane Downes 2018 Co-Supervisor Peptide Synthesis for Biological Application Doctor of Philosophy Doctorate Full Time Miss Aimee Jade Horsfall 2018 Co-Supervisor Application of organic synthetic chemistry and computational techniques for drug discovery and optimization Doctor of Philosophy Doctorate Full Time Miss Hebatalla Ahmed Ibrahim Mohamed 2017 Principal Supervisor Protein Crystallography Doctor of Philosophy Doctorate Full Time Miss Rebecca Louise Frkic 2017 Principal Supervisor Protein Crystallography of PPAR Gamma and Estrogen Receptor With Various Novel Drug Candidates Master of Philosophy Master Full Time Mr Benjamin Soon Kai Chua 2017 Co-Supervisor Using Grapevine enzymes to make novel biochemicals Master of Philosophy Master Full Time Miss Emi Kaye Schutz 2016 Co-Supervisor The role of Eukaryotic Elongation Factor 2 Kinase (EEF2K) in the Survival of cancer cells and Macrophages Doctor of Philosophy Doctorate Full Time Mr Roman Valeryevich Lenchine -
Past Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2018 - 2019 Co-Supervisor Structural and Biophysical Characterisation of Mycobacterium tuberculosis Dethiobiotin Synthetase Doctor of Philosophy Doctorate Full Time Andrew Philip Thompson 2016 - 2017 Principal Supervisor Defining the Key Residues in PCNA-Protein Interactions Master of Philosophy Master Part Time Alice Jane Kroker 2014 - 2018 Principal Supervisor Structural and Biochemical Studies on Three Aspergillus fumigatus Proteins That Present as Targets for Novel Antifungal Drugs Doctor of Philosophy Doctorate Full Time Andrew Craig Marshall 2014 - 2016 Co-Supervisor Molecular and Structural Characterization of the Candidate Enzymes Responsible for Tartaric Acid Synthesis in the Grapevine Doctor of Philosophy Doctorate Full Time Mr Yong Jia
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