Dr John Bruning

Senior Lecturer

School of Biological Sciences

Faculty of Sciences, Engineering and Technology

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

I am an expert in structural biology of proteins and protein interactions who has solved the crystal structures of dozens of proteins and protein-ligand complexes. My research has been published in high impact journals such as Nature, Nature Chemical Biology, Nature Communications, Nature Structural and Molecular Biology, and Angewandte Chemie, and has published ~120 manuscripts. The Laboratory of Protein Crystallography at UoA is one of the few in the world that can purify human PCNA in the quantities and quality necessary for structural studies.

My Research
Career
Publications
Grants and Funding
Teaching
Supervision
Contact

I head the Laboratory of Protein Crystallography which studeis Nuclear Receptors, Microbial Enzymology, and Broad-Based Cancer Therapeutics. Understanding human disease requires knowledge of the structure-function relationships in protein sensor modulation.This knowledge is incomplete on a broad spectrum of biological systems including nuclear receptor (NR) signalling, proteins involved in cancer biology, and pathogen protein machinery. Our research is focused on understanding these classes of protein interactions on an atomic level. In addition to gaining a basic understanding of protein mechanism, we also focus on structure guided drug design. In our work in understanding NR signalling, we have uncovered new paradigms in NR allostery. These allosteric modulations can now be further studied on a broader scale and harnessed to employ structure guided drug design. Work in the tuberculosis (TB) structural biology consortium has led to a quantum leap in understanding enzymology of the pathogen allowing for new chemotherapy development. We wish to use similar methodology to study a fungal pathogen for the discovery of new anti-fungal molecules.Our research with proteins of the human replication fork, such as PCNA (Proliferating Cellular Nuclear Antigen) has uncovered atomic determinants of these protein modulators that can be used as new paradigms for cancer therapeutic targeting. We will continue this research to aid in the discovery of alternative cancer therapeutic targets and seek to understand their structure-function relationship.

Appointments

Date	Position	Institution name
2024 - ongoing	Associate Professor	The University of Adelaide
2021 - ongoing	Editor	Journal PPAR research
2016 - ongoing	Adjunct Professor	The Scripps Research Institute
2016 - ongoing	Senior Lecturer Level C (Tenured)	The University of Adelaide
2016 - ongoing	Head of the Laboratory of Protein Crystallography	The University of Adelaide
2012 - 2015	Lecturer Level B	The University of Adelaide
2007 - 2012	Associate Research Scientist	Texas A&M University
2005 - 2007	Post-doctoral Research Associate	Scripps Research Institute

Education

Date Institution name Country Title

1998 - 2004 Rice University United States PhD

1994 - 1997 Texas A&M University USA BSc
Postgraduate Training

Date Title Institution Country

2007 - 2012 Assistant Research Scientist Texas A&M USA

2005 - 2007 Post-doctoral Research Assistant The Scripps Research Institute USA

Date	Institution name	Country	Title
1998 - 2004	Rice University	United States	PhD
1994 - 1997	Texas A&M University	USA	BSc

Date	Title	Institution	Country
2007 - 2012	Assistant Research Scientist	Texas A&M	USA
2005 - 2007	Post-doctoral Research Assistant	The Scripps Research Institute	USA

Journals

Year	Citation
2025	Kirk, A. M., Stok, J. E., Wong, S. H., Coleman, T., Challinor, V. L., Herring, J. N., . . . De Voss, J. J. (2025). An Asynchronous, Concerted Mechanism for Cytochrome P450-Catalyzed Dehydrogenation: A Combined Deuterium Labeling and QM/MM Study. ACS Catalysis, 15(2), 1274-1286. DOI
2025	Podgorski, M. N., Martínez-Castro, L., Bruning, J. B., Campbell, E. C., Maréchal, J. -D., & Bell, S. G. (2025). Investigating the Correlation between Product Release and Solvation in Cytochrome P450 Enzymes. ACS Catalysis, 15(4), 2867-2884. DOI
2025	Holroyd, D. L., Kumar, A., Vasquez, E., Masic, V., von Itzstein, M., Bruning, J. B., & Hansman, G. S. (2025). Antigenic structural analysis of bat and human norovirus protruding (P) domains.. Journal of virology, 99(4), e0197124. DOI
2025	Pederick, J. L., Kumar, A., Pukala, T. L., & Bruning, J. B. (2025). Functional and structural characterization of Staphylococcus aureus N-acetylglucosamine 1-phosphate uridyltransferase (GlmU) reveals a redox-sensitive acetyltransferase activity. Protein Science, 34(4), 11 pages. DOI
2024	Lee, J. H. Z., Bruning, J. B., & Bell, S. G. (2024). An In Crystallo Reaction with an Engineered Cytochrome P450 Peroxygenase*. Chemistry: A European Journal*, 30(8), e202303335-1-e202303335-8. DOI Scopus2
2024	Clayton-Cuch, D., Yu, L., McDougal, D., Burbidge, C. A., Bruning, J. B., Bradley, D., . . . Bulone, V. (2024). Biochemical and in silico characterization of glycosyltransferases from red sweet cherry (Prunus avium L.) reveals their broad specificity toward phenolic substrates. Food Chemistry: Molecular Sciences, 8, 100193. DOI Scopus4
2024	Mohamed, H., Child, S. A., Doherty, D. Z., Bruning, J. B., & Bell, S. G. (2024). Structural determination and characterisation of the CYP105Q4 cytochrome P450 enzyme from Mycobacterium marinum. Arch Biochem Biophys, 754, 109950-1-109950-11. DOI Scopus1
2024	Pederick, J. L., Frkic, R. L., McDougal, D. P., & Bruning, J. B. (2024). A structural basis for the activation of peroxisome proliferator-activated receptor gamma (PPARγ) by perfluorooctanoic acid (PFOA). Chemosphere, 354, 141723-1-141723-12. DOI Scopus1 Europe PMC2
2024	Wallach, I., Bernard, D., Nguyen, K., Ho, G., Morrison, A., Stecula, A., . . . Taglialatela, A. (2024). AI is a viable alternative to high throughput screening: a 318-target study. Scientific Reports, 14(1), 16 pages. DOI Scopus30 Europe PMC32
2024	Podgorski, M. N., Akter, J., Churchman, L. R., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2024). Engineering Peroxygenase Activity into Cytochrome P450 Monooxygenases through Modification of the Oxygen Binding Region. ACS Catalysis, 14(10), 7426-7443. DOI Scopus8
2024	Lee, J. H. Z., Coleman, T., Mclean, M. A., Podgorski, M. N., Hayball, E. F., Stone, I. S. J., . . . Bell, S. G. (2024). Selective α-Hydroxyketone Formation and Subsequent C-C Bond Cleavage by Cytochrome P450 Monooxygenase Enzymes. ACS Catalysis, 14(11), 8958-8971. DOI Scopus3 Europe PMC1
2024	Giles, E., Heifets, A., Artía, Z., Inde, Z., Liu, Z., Zhang, Z., . . . Waggoner, S. N. (2024). Correction to: AI is a viable alternative to high throughput screening: a 318-target study (Scientific Reports, (2024), 14, 1, (7526), 10.1038/s41598-024-54655-z). Scientific Reports, 14(1). DOI
2024	Pederick, J. L., Vandborg, B. C., George, A., Bovermann, H., Boyd, J. M., Freundlich, J. S., & Bruning, J. B. (2024). Identification of cysteine metabolism regulator (CymR)-derived pentapeptides as nanomolar inhibitors of Staphylococcus aureus O-acetyl-ʟ-serine sulfhydrylase (CysK).. bioRxiv. DOI
2024	Pederick, J. L., Vandborg, B. C., George, A., Bovermann, H., Boyd, J. M., Freundlich, J. S., & Bruning, J. B. (2024). Identification of Cysteine Metabolism Regulator (CymR)-Derived Pentapeptides as Nanomolar Inhibitors of Staphylococcus aureus O-Acetyl-l-serine Sulfhydrylase (CysK).. ACS Infect Dis, 11(1), 238-248. DOI
2023	Vandborg, B., Holroyd, D. L., Pukala, T., & Bruning, J. B. (2023). Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization. Protein Expression and Purification, 212, 1-7. DOI Scopus1 Europe PMC1
2023	Salaemae, W., Thompson, A. P., Gaiser, B. I., Lee, K. J., Huxley, M. T., Sumby, C. J., . . . Wegener, K. L. (2023). Fortuitous <i>In Vitro</i> Compound Degradation Produces a Tractable Hit against <i>Mycobacterium tuberculosis</i> Dethiobiotin Synthetase: A Cautionary Tale of What Goes In Does Not Always Come Out. ACS Chemical Biology, 18(9), 1985-1992. DOI
2023	Podgorski, M. N., Lee, J. H. Z., Harbort, J. S., Nguyen, G. T. H., Doherty, D. Z., Donald, W. A., . . . Bell, S. G. (2023). Characterisation of the heme aqua-ligand coordination environment in an engineered peroxygenase cytochrome P450 variant. Journal of Inorganic Biochemistry, 249, 1-13. DOI Scopus3
2023	Becker, R., Pederick, J. L., Dawes, E. G., Bruning, J. B., & Abell, A. D. (2023). Structure-guided design and synthesis of ATP-competitive N-acyl-substituted sulfamide D-alanine-D-alanine ligase inhibitors. Bioorganic and Medicinal Chemistry, 96, 14 pages. DOI
2023	Vandborg, B. C., Horsfall, A. J., Pederick, J. L., Abell, A. D., & Bruning, J. B. (2023). Towards a High-Affinity Peptidomimetic Targeting Proliferating Cell Nuclear Antigen from Aspergillus fumigatus. Journal of Fungi, 9(11), 16 pages. DOI
2023	Henderson, S. W., Nakayama, Y., Whitelaw, M. L., Bruning, J. B., Anderson, P. A., Tyerman, S. D., . . . Yool, A. J. (2023). Proteoliposomes reconstituted with human aquaporin-1 reveal novel single ion channel properties. Biophysical Reports, 3(1), 100100-1-100100-15. DOI Scopus3 WoS2 Europe PMC2
2023	Pederick, J. L., Klose, J., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2023). <i>Escherichia coli</i> YgiC and YjfC Possess Peptide─Spermidine Ligase Activity. Biochemistry, 62(4), 899-911. DOI Scopus3 Europe PMC2
2023	Stachura, D. L., Nguyen, S., Polyak, S. W., Jovcevski, B., Bruning, J. B., & Abell, A. D. (2023). Structural Study of Potent Triazole-Based Inhibitors of <i>Staphylococcus aureus</i> Biotin Protein Ligase. ACS Medicinal Chemistry Letters, 14(3), 285-290. DOI Scopus1 Europe PMC1
2023	Ghith, A., Bruning, J. B., & Bell, S. G. (2023). The catalytic activity and structure of the lipid metabolizing CYP124 cytochrome P450 enzyme from Mycobacterium marinum.. Archives of biochemistry and biophysics, 737, 109554. DOI Scopus2 WoS1
2023	Salaemae, W., Thompson, A., Gaiser, B., Lee, K. J., Huxley, M., Sumby, C., . . . Wegener, K. (2023). Fortuitous<i>in vitro</i>compound degradation produces a tractable hit against<i>Mycobacterium tuberculosis</i>dethiobiotin synthetase: a cautionary tale of what goes in, does not always come out. DOI
2023	Miller, J. C., Lee, J. H. Z., Mclean, M. A., Chao, R. R., Stone, I. S. J., Pukala, T. L., . . . Bell, S. G. (2023). Engineering C–C Bond Cleavage Activity into a P450 Monooxygenase Enzyme. Journal of the American Chemical Society, 145(16), 9207-9222. DOI Scopus15 WoS2 Europe PMC7
2023	Coleman, T., Podgorski, M. N., Doyle, M. L., Scaffidi-Muta, J. M., Campbell, E. C., Bruning, J. B., . . . Bell, S. G. (2023). Cytochrome P450-catalyzed oxidation of halogen-containing substrates.. Journal of Inorganic Biochemistry, 244, 12 pages. DOI Scopus5 WoS1 Europe PMC2
2023	Frkic, R. L., Pederick, J. L., Horsfall, A. J., Jovcevski, B., Crame, E. E., Kowalczyk, W., . . . Bruning, J. B. (2023). PPARγ Corepression Involves Alternate Ligand Conformation and Inflation of H12 Ensembles. ACS Chemical Biology, 18(5), 1115-1123. DOI Scopus3 Europe PMC6
2023	Ghith, A., Bruning, J. B., & Bell, S. G. (2023). The oxidation of cholesterol derivatives by the CYP124 and CYP142 enzymes from Mycobacterium marinum.. J Steroid Biochem Mol Biol, 231, 106317-1-106317-12. DOI Scopus1 Europe PMC1
2023	Pantong, W., Pederick, J. L., Maenpuen, S., Tinikul, R., Jayapalan, J. J., Jovcevski, B., . . . Salaemae, W. (2023). Biochemical and Structural Characterization of Meningococcal Methylenetetrahydrofolate Reductase. Protein Science, 32(6), e4654-1-e4654-13. DOI Scopus1 Europe PMC2
2023	Clayton-Cuch, D., McDougal, D., Schwerdt, J. G., Yu, L., Shirley, N., Bradley, D., . . . Bulone, V. (2023). Identification and characterisation of MdUGT78T2 as a galactosyltransferase with dual activity on flavonol and anthocyanidin substrates in red-skinned apple fruit (Malus domestica L.). Food Chemistry, 424, 12 pages. DOI Scopus5 Europe PMC3
2023	Podgorski, M. N., Keto, A. B., Coleman, T., Bruning, J. B., De Voss, J. J., Krenske, E. H., & Bell, S. G. (2023). The Oxidation of Oxygen and Sulfur-Containing Heterocycles by Cytochrome P450 Enzymes. Chemistry: A European Journal, 29(50), e202301371-1-e202301371-15. DOI Scopus5 Europe PMC1
2023	Horsfall, A. J., Chav, T., Pederick, J. L., Kikhtyak, Z., Vandborg, B. C., Kowalczyk, W., . . . Bruning, J. B. (2023). Designing Fluorescent Nuclear Permeable Peptidomimetics to Target Proliferating Cell Nuclear Antigen. Journal of Medicinal Chemistry, 66(15), 10354-10363. DOI
2023	McDougal, D. P., Rajapaksha, H., Pederick, J. L., & Bruning, J. B. (2023). warpDOCK: Large-Scale Virtual Drug Discovery Using Cloud Infrastructure. ACS Omega, 8(32), 28932-29846. DOI Scopus2 Europe PMC1
2023	Pederick, J. L., Woolman, J. C., & Bruning, J. B. (2023). Comparative functional and structural analysis of Pseudomonas aeruginosa ᴅ-alanine-ᴅ-alanine ligase isoforms as prospective antibiotic targets.. The FEBS journal, 290(23), 5536-5553. DOI
2022	Coleman, T., Kirk, A. M., Lee, J. H. Z., Doherty, D. Z., Bruning, J. B., Krenske, E. H., . . . Bell, S. G. (2022). Different geometric requirements for cytochrome P450-catalyzed aliphatic versus aromatic hydroxylation results in chemoselective oxidation. ACS Catalysis, 12(2), 1258-1267. DOI Scopus24 WoS11
2022	Podgorski, M. N., Harbort, J. S., Lee, J. H. Z., Nguyen, G. T. H., Bruning, J. B., Donald, W. A., . . . Bell, S. G. (2022). An altered heme environment in an engineered cytochrome P450 enzyme enables the switch from monooxygenase to peroxygenase activity. ACS Catalysis, 12(3), 1614-1625. DOI Scopus40 WoS17
2022	Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2022). Structural insights into the antifungal drug target guanosine monophosphate synthase from Aspergillus fumigatus. Acta Crystallographica Section D Structural Biology, 78(2), 1-12. DOI Scopus5 WoS2 Europe PMC3
2022	Bersten, D. C., Sullivan, A. E., McDougal, D., Breen, J., Fitzsimmons, R. L., Muscat, G. E. O., . . . Whitelaw, M. L. (2022). Core and Flanking bHLH-PAS:DNA interactions mediate specificity and drive obesity. DOI Europe PMC1
2022	Nguyen, S., Jovcevski, B., Truong, J. Q., Pukala, T. L., & Bruning, J. B. (2022). A structural model of the human plasminogen and Aspergillus fumigatus enolase complex.. Proteins, 90(8), 1509-1520. DOI Scopus1 Europe PMC1
2022	Mohamed, H., Child, S. A., Bruning, J. B., & Bell, S. G. (2022). A comparison of the bacterial CYP51 cytochrome P450 enzymes from Mycobacterium marinum and Mycobacterium tuberculosis. The Journal of Steroid Biochemistry and Molecular Biology, 221, 106097-1-106097-14. DOI Scopus5 WoS3 Europe PMC1
2022	Button, E. L., Rossi, J. J., McDougal, D. P., Bruning, J. B., Peet, D., Bersten, D., . . . Whitelaw, M. (2022). Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.. The Biochemical journal, 479(13), 1441-1454. DOI Scopus3 Europe PMC2
2022	Downes, C. E., McClure, B. J., McDougal, D. P., Heatley, S. L., Bruning, J. B., Thomas, D., . . . White, D. L. (2022). JAK2 Alterations in Acute Lymphoblastic Leukemia: Molecular Insights for Superior Precision Medicine Strategies. Frontiers in Cell and Developmental Biology, 10, 1-34. DOI Scopus21 WoS8 Europe PMC15
2022	Ghith, A., Doherty, D. Z., Bruning, J. B., Russell, R. A., De Voss, J. J., & Bell, S. G. (2022). The Structures of the Steroid Binding CYP142 Cytochrome P450 Enzymes from Mycobacterium ulcerans and Mycobacterium marinum. ACS Infectious Diseases, 8(8), 1606-1617. DOI Scopus7 WoS3 Europe PMC1
2022	Pederick, J. L., Horsfall, A. J., Jovcevski, B., Klose, J., Abell, A. D., Pukala, T. L., & Bruning, J. B. (2022). Discovery of an ʟ-amino acid ligase implicated in Staphylococcal sulfur amino acid metabolism. Journal of Biological Chemistry, 298(10), 1-16. DOI Scopus5 WoS1 Europe PMC3
2022	Coleman, T., Lee, J., Kirk, A. M., Doherty, D. Z., Podgorski, M. N., Pinidiya, D. K., . . . Bell, S. G. (2022). Enabling Aromatic Hydroxylation in a Cytochrome P450 Monooxygenase Enzyme through Protein Engineering. Chemistry: A European Journal, 28(67), 1-13. DOI Scopus16 WoS2 Europe PMC5
2022	Podgorski, M. N., Coleman, T., Churchman, L. R., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2022). Investigating the active oxidants involved in cytochrome P450 catalyzed sulfoxidation reactions. Chemistry: A European Journal, 28(72), 1-14. DOI Scopus14 WoS5 Europe PMC7
2022	Coleman, T., Doherty, D. Z., Zhang, T., Podgorski, M. N., Qiao, R., Lee, J. H. Z., . . . Bell, S. G. (2022). Exploring the Factors which Result in Cytochrome P450 Catalyzed Desaturation Versus Hydroxylation. Chemistry: An Asian Journal, 17(24), 1-15. DOI Scopus12 WoS4 Europe PMC4
2022	Stachura, D. L., Nguyen, S., Polyak, S. W., Jovcevski, B., Bruning, J. B., & Abell, A. D. (2022). A New 1,2,3-Triazole Scaffold with Improved Potency against Staphylococcus aureus Biotin Protein Ligase. ACS Infectious Diseases, 8(12), 2579-2585. DOI Scopus5 WoS2 Europe PMC2
2021	Pederick, J. L., & Bruning, J. B. (2021). An antimony-phosphomolybdate microassay of ATPase activity through the detection of inorganic phosphate. Analytical Biochemistry, 623, 114170. DOI Scopus9 WoS4 Europe PMC2
2021	Horsfall, A. J., Chav, T., Bruning, J. B., & Abell, A. D. (2021). A turn-on fluorescent PCNA sensor. Bioorganic & Medicinal Chemistry Letters, 41, 128031-1-128031-6. DOI
2021	Xie, J., De Poi, S. P., Humphrey, S. J., Hein, L. K., Bruning, J. B., Pan, W., . . . Proud, C. G. (2021). TSC-insensitive Rheb mutations induce oncogenic transformation through a combination of constitutively active mTORC1 signalling and proteome remodelling. Cellular and Molecular Life Sciences, 78(8), 4035-4052. DOI Scopus6 WoS2 Europe PMC3
2021	Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid. Acta Crystallographica Section F Structural Biology Communications, 77(5), 156-162. DOI Scopus2 WoS2 Europe PMC2
2021	Tavakoli Shirazi, P., Eadie, L. N., Page, E. C., Heatley, S. L., Bruning, J. B., & White, D. L. (2021). Constitutive JAK/STAT signaling is the primary mechanism of resistance to JAKi in TYK2-rearranged acute lymphoblastic leukemia. Cancer Letters, 512, 28-37. DOI Scopus10 WoS8 Europe PMC7
2021	Horsfall, A. J., Vandborg, B. A., Kowalczyk, W., Chav, T., Scanlon, D. B., Abell, A. D., & Bruning, J. B. (2021). Unlocking the PIP-box: A peptide library reveals interactions that drive high affinity binding to human PCNA.. The Journal of biological chemistry, 296, 1-16. DOI Scopus11 WoS6 Europe PMC12
2021	Horsfall, A. J., McDougal, D. P., Scanlon, D. B., Bruning, J. B., & Abell, A. D. (2021). Approaches to introduce helical structure in cysteine-containing peptides with a bimane group. Chembiochem, 22(17), 2711-2720. DOI Scopus4 WoS3 Europe PMC1
2021	Qu, Y., Zhang, B., Wang, Y., Yin, S., Pederick, J. L., Bruning, J. B., . . . Bi, J. (2021). Immunogenicity study of engineered ferritins with C- and N-terminus insertion of Epstein-Barr nuclear antigen 1 epitope. Vaccine, 39(34), 4830-4841. DOI Scopus8 WoS4 Europe PMC3
2021	Downes, C. E. J., McClure, B. J., Bruning, J. B., Page, E., Breen, J., Rehn, J., . . . White, D. L. (2021). Acquired JAK2 mutations confer resistance to JAK inhibitors in cell models of acute lymphoblastic leukemia. NPJ Precision Oncology, 5(1), 1-13. DOI Scopus18 WoS8 Europe PMC11
2021	Frkic, R. L., Richter, K., & Bruning, J. B. (2021). The therapeutic potential of inhibiting PPARγ phosphorylation to treat type 2 diabetes.. The Journal of biological chemistry, 297(3), 101030. DOI Scopus45 WoS21 Europe PMC23
2021	Marshall, A. C., & Bruning, J. B. (2021). Engineering potassium activation into biosynthetic thiolase. The Biochemical journal, 478(15), 3047-3062. DOI Scopus2 Europe PMC1
2021	Chao, R., Lau, I., Coleman, T., Churchman, L. R., Child, S. A., Lee, J. H. Z., . . . Bell, S. G. (2021). The stereoselective oxidation of para-substituted benzenes by a cytochrome P450 biocatalyst. Chemistry: A European Journal, 27(59), 14765-14777. DOI Scopus9 WoS6 Europe PMC2
2021	Horsfall, A. J., Vandborg, B., Kikhtyak, Z., Scanlon, D. B., Tilley, W. D., Hickey, T. E., . . . Abell, A. D. (2021). A cell permeable bimane-constrained PCNA-interacting peptide. RSC Chemical Biology, 2(5), 1499-1508. DOI Scopus5 WoS3 Europe PMC2
2021	Schumann, N. C., Lee, K. J., Thompson, A. P., Salaemae, W., Pederick, J. L., Avery, T., . . . Abell, A. D. (2021). Inhibition of Mycobacterium tuberculosis dethiobiotin synthase (MtDTBS): toward next-generation antituberculosis agents. ACS Chemical Biology, 16(11), 2339-2347. DOI Scopus7 WoS3 Europe PMC6
2021	Podgorski, M. N., Coleman, T., Giang, P. D., Wang, C. R., Bruning, J. B., Bernhardt, P. V., . . . Bell, S. G. (2021). To be, or not to be, an Inhibitor: a comparison of azole interactions with and oxidation by a cytochrome P450 enzyme. Inorganic Chemistry, 61(1), 236-245. DOI Scopus13 WoS4 Europe PMC4
2021	Slynko, I., Nguyen, S., Hamilton, E. M. C., Wisse, L. E., de Esch, I. J. P., de Graaf, C., . . . van der Knaap, M. S. (2021). Vanishing white matter: Eukaryotic initiation factor 2B model and the impact of missense mutations.. Molecular genetics & genomic medicine, 9(3), 1-15. DOI Scopus21 WoS14 Europe PMC12
2021	Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2021). Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase.. The FEBS journal, 288(7), 2398-2417. DOI Scopus8 WoS6 Europe PMC6
2021	Nguyen, S., Truong, J. Q., & Bruning, J. B. (2021). Targeting Unconventional Pathways in Pursuit of Novel Antifungals.. Front Mol Biosci, 7, 1-16. DOI Scopus14 WoS6 Europe PMC6
2021	Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Derivatization of protein crystals with I3C using random microseed matrix screening. Journal of Visualized Experiments, 2021(167), 1-19. DOI WoS1 Europe PMC1
2021	Coleman, T., Kirk, A. M., Chao, R. R., Podgorski, M. N., Harbort, J. S., Churchman, L. R., . . . Bell, S. G. (2021). Understanding the Mechanistic Requirements for Efficient and Stereoselective Alkene Epoxidation by a Cytochrome P450 Enzyme. ACS Catalysis, 11(4), 1995-2010. DOI Scopus41 WoS21
2020	Jia, Y., Shen, G., Nguyen, S., Zhang, Y., Huang, K. -S., Ho, H. -Y., . . . Wang, W. -L. (2020). Analysis of the mutation dynamics of SARS-CoV-2 reveals the spread history and emergence of RBD mutant with lower ACE2 binding affinity. DOI Europe PMC2
2020	Horsfall, A. J., Abell, A. D., & Bruning, J. (2020). Targeting PCNA with peptide mimetics for therapeutic purposes. ChemBioChem, 21(4), 442-450. DOI Scopus31 WoS15 Europe PMC19
2020	Podgorski, M. N., Coleman, T., Chao, R. R., De Voss, J. J., Bruning, J. B., & Bell, S. G. (2020). Investigation of the requirements for efficient and selective cytochrome P450 monooxygenase catalysis across different reactions. Journal of Inorganic Biochemistry, 203, 110913-1-110913-13. DOI Scopus29 WoS19 Europe PMC14
2020	Podgorski, M. N., Harbort, J. S., Coleman, T., Stok, J. E., Yorke, J. A., Wong, L. -L., . . . Bell, S. G. (2020). Biophysical techniques for distinguishing ligand binding modes in cytochrome P450 monooxygenases. Biochemistry, 59(9), 1038-1050. DOI Scopus24 WoS15 Europe PMC11
2020	Coleman, T., Stok, J. E., Podgorski, M. N., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2020). Structural insights into the role of the acid-alcohol pair of residues required for dioxygen activation in cytochrome P450 enzymes. JBIC Journal of Biological Inorganic Chemistry, 25(4), 583-596. DOI Scopus33 WoS24 Europe PMC13
2020	Pederick, J. L., Thompson, A. P., Bell, S. G., & Bruning, J. B. (2020). D-alanine-D-alanine ligase as a model for the activation of ATP-grasp enzymes by monovalent cations.. The Journal of biological chemistry, 295(23), 7894-7904. DOI Scopus23 WoS15 Europe PMC14
2020	Child, S. A., Ghith, A., Bruning, J. B., & Bell, S. G. (2020). A comparison of steroid and lipid binding cytochrome P450s from Mycobacterium marinum and Mycobacterium tuberculosis. Journal of Inorganic Biochemistry, 209, 1-12. DOI Scopus13 WoS8 Europe PMC2
2020	Xie, J., De Poi, S., Humphrey, S., Hein, L., Bruning, J., Pan, W., . . . Proud, C. (2020). TSC-Insensitive Rheb Mutations Induce Oncogenic Transformation Through a Combination of Hyperactive mTORC1 Signalling and Metabolic Reprogramming. DOI
2019	Truong, J. Q., Panjikar, S., Shearwin-Whyatt, L., Bruning, J. B., & Shearwin, K. E. (2019). Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68. Acta crystallographica Section D. Structural biology, 75(7), 670-681. DOI Scopus3 WoS3 Europe PMC3
2019	Lee, K. J., Tieu, W., Blanco-Rodriguez, B., Paparella, A. S., Yu, J., Hayes, A., . . . Abell, A. D. (2019). Sulfonamide-based inhibitors of biotin protein ligase as new antibiotic leads. ACS Chemical Biology, 14(9), 1990-1997. DOI Scopus9 WoS4 Europe PMC2
2019	Jia, Y., Burbidge, C. A., Sweetman, C., Schutz, E., Soole, K., Jenkins, C., . . . Ford, C. M. (2019). An aldo-keto reductase with 2-keto- L-gulonate reductase activity functions in L-tartaric acid biosynthesis from vitamin C in Vitis vinifera. The Journal of biological chemistry, 294(44), 15932-15946. DOI Scopus20 WoS12 Europe PMC11
2019	Schumann, N. C., Bruning, J., Marshall, A. C., & Abell, A. D. (2019). The role of N-terminal heterocycles in hydrogen bonding to α-chymotrypsin. Bioorganic and Medicinal Chemistry Letters, 29(3), 396-399. DOI Scopus3 WoS2 Europe PMC1
2019	Marshall, A. C., Kidd, S. E., Lamont-Friedrich, S. J., Arentz, G., Hoffmann, P., Coad, B. R., & Bruning, J. B. (2019). Structure, mechanism, and inhibition of aspergillus fumigatus thioredoxin reductase. Antimicrobial Agents and Chemotherapy, 63(3), 1-15. DOI Scopus30 WoS25 Europe PMC17
2019	Elzahhar, P. A., Alaaeddine, R., Ibrahim, T. M., Nassra, R., Ismail, A., Chua, B. S. K., . . . Belal, A. S. F. (2019). Shooting three inflammatory targets with a single bullet: Novel multi-targeting anti-inflammatory glitazones. European Journal of Medicinal Chemistry, 167, 562-582. DOI Scopus39 WoS28 Europe PMC12
2019	Child, S. A., Flint, K. L., Bruning, J. B., & Bell, S. G. (2019). The characterisation of two members of the cytochrome P450 CYP150 family: CYP150A5 and CYP150A6 from Mycobacterium marinum. Biochimica et Biophysica Acta - General Subjects, 1863(5), 925-934. DOI Scopus4 WoS4
2019	Chang, M. R., Ciesla, A., Strutzenberg, T. S., Novick, S. J., He, Y., Garcia-Ordonez, R. D., . . . Griffin, P. R. (2019). Unique Polypharmacology Nuclear Receptor Modulator Blocks Inflammatory Signaling Pathways. ACS Chemical Biology, 14(5), 1051-1062. DOI Scopus8 WoS8 Europe PMC4
2018	Marshall, A., Keiller, B., Pederick, J., Abell, A., & Bruning, J. (2018). Crystal structure of bovine alpha-chymotrypsin in space group P65. Crystals, 8(12), 460. DOI Scopus3 WoS1
2018	Frkic, R. L., Chua, B. S., Shin, Y., Pascal, B. D., Novick, S. J., Kamenecka, T. M., . . . Bruning, J. B. (2018). Structural and dynamic elucidation of a non-acid PPARγ partial agonist: SR1988. Nuclear Receptor Research, 5, 101350-1-101350-12. DOI Europe PMC3
2018	Child, S., Naumann, E., Bruning, J., & Bell, S. (2018). Structural and functional characterisation of the cytochrome P450 enzyme CYP268A2 from Mycobacterium marinum. The Biochemical Journal, 475(4), 705-722. DOI Scopus14 WoS11 Europe PMC4
2018	Marshall, A., Bond, C., & Bruning, J. (2018). Structure of Aspergillus fumigatus Cytosolic thiolase: trapped tetrahedral reaction intermediates and activation by monovalent cations. ACS Catalysis, 8(3), 1973-1989. DOI Scopus4 WoS3
2018	Coleman, T., Wong, S., Podgorski, M., Bruning, J., De Voss, J., & Bell, S. (2018). Cytochrome P450 CYP199A4 from Rhodopseudomonas palustris catalyses heteroatom dealkylations, sulfoxidation and amide and cyclic hemiacetal formation. ACS Catalysis, 8(7), 5915-5927. DOI Scopus35 WoS22
2018	Wegener, K. L., McGrath, A. E., Dixon, N. E., Oakley, A. J., Scanlon, D. B., Abell, A. D., & Bruning, J. (2018). Rational design of a 310-helical PIP-box mimetic targeting PCNA - the human sliding clamp. Chemistry - A European Journal, 24(44), 11325-11331. DOI Scopus16 WoS10 Europe PMC9
2018	Thompson, A., Wegener, K., Booker, G., Polyak, S., & Bruning, J. (2018). Precipitant-ligand exchange technique reveals the ADP binding mode in Mycobacterium tuberculosis dethiobiotin synthetase. Acta Crystallographica Section D: Structural Biology, 74(10), 965-972. DOI Scopus4 WoS4 Europe PMC1
2018	Frkic, R. L., Marshall, A. C., Blayo, A. -L., Pukala, T. L., Kamenecka, T. M., Griffin, P. R., & Bruning, J. B. (2018). PPARγ in complex with an antagonist and inverse agonist: a tumble and trap mechanism of the activation helix. iScience, 5, 69-79. DOI Scopus51 WoS31 Europe PMC31
2018	Zheng, J., Corzo, C., Chang, M., Shang, J., Lam, V., Brust, R., . . . Griffin, P. (2018). Chemical crosslinking mass spectrometry reveals the conformational landscape of the activation helix of PPARγ; a model for ligand-dependent antagonism. Structure, 26(11), 1431-1439. DOI Scopus26 WoS18 Europe PMC21
2018	Thompson, A. P., Salaemae, W., Pederick, J. L., Abell, A. D., Booker, G. W., Bruning, J. B., . . . Polyak, S. W. (2018). Mycobacterium tuberculosis Dethiobiotin Synthetase Facilitates Nucleoside Triphosphate Promiscuity through Alternate Binding Modes. ACS Catalysis, 8(11), 10774-10783. DOI Scopus7 WoS5
2018	Wegener, K. L., McGrath, A. E., Dixon, N. E., Oakley, A. J., Scanlon, D. B., Abell, A. D., & Bruning, J. B. (2018). Front Cover: Rational Design of a 3<sub>10</sub>‐Helical PIP‐Box Mimetic Targeting PCNA, the Human Sliding Clamp (Chem. Eur. J. 44/2018). Chemistry – A European Journal, 24(44), 11234. DOI
2017	Stevens, T., Song, S., Bruning, J., Choo, A., & Baxter, S. (2017). Expressing a moth abcc2 gene in transgenic Drosophila causes susceptibility to Bt Cry1Ac without requiring a cadherin-like protein receptor. Insect Biochemistry and Molecular Biology, 80, 61-70. DOI Scopus46 WoS39 Europe PMC26
2017	Marshall, A., Kroker, A., Murray, L., Gronthos, K., Rajapaksha, H., Wegener, K., & Bruning, J. (2017). Structure of the sliding clamp from the fungal pathogen Aspergillus fumigatus (AfumPCNA) and interactions with Human p21. FEBS Journal, 284(6), 985-1002. DOI Scopus11 WoS9 Europe PMC9
2017	Sternicki, L., Wegener, K., Bruning, J., Booker, G., & Polyak, S. (2017). Mechanisms governing precise protein biotinylation. Trends in Biochemical Sciences, 42(5), 383-394. DOI Scopus22 WoS17 Europe PMC9
2017	Rajapaksha, H., Bhatia, H., Wegener, K., Petrovsky, N., & Bruning, J. (2017). X-ray crystal structure of rivoglitazone bound to PPARγ and PPAR subtype selectivity of TZDs. Biochimica et Biophysica Acta - General Subjects, 1861(8), 1981-1991. DOI Scopus17 WoS16 Europe PMC10
2017	Frkic, R., He, Y., Rodriguez, B., Chang, M., Kuruvilla, D., Ciesla, A., . . . Bruning, J. (2017). Structure-Activity Relationship of 2,4-Dichloro-N-(3,5-dichloro-4-(quinolin-3-yloxy)phenyl)benzenesulfonamide (INT131) Analogs for PPARγ-Targeted Antidiabetics. Journal of Medicinal Chemistry, 60(11), 4584-4593. DOI Scopus24 WoS18 Europe PMC11
2017	Zheng, J., Chang, M., Stites, R., Wang, Y., Bruning, J., Pascal, B., . . . Griffin, P. (2017). HDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions. Nature Communications, 8(1), 923-1-923-13. DOI Scopus36 WoS26 Europe PMC27
2016	Van Marrewijk, L., Polyak, S., Hijnen, M., Kuruvilla, D., Chang, M., Shin, Y., . . . Bruning, J. (2016). SR2067 reveals a unique kinetic and structural signature for PPARγ partial agonism. ACS Chemical Biology, 11(1), 273-283. DOI Scopus33 WoS26 Europe PMC17
2016	Zhang, X., Bruning, J., George, J., & Abell, A. (2016). A mechanistic study on the inhibition of α-chymotrypsin by a macrocyclic peptidomimetic aldehyde. Organic and Biomolecular Chemistry, 14(29), 6970-6978. DOI Scopus12 WoS10 Europe PMC1
2016	Stechschulte, L., Czernik, P., Rotter, Z., Tausif, F., Corzo, C., Marciano, D., . . . Lecka-Czernik, B. (2016). PPARG Post-translational modifications regulate bone formation and bone resorption. EBioMedicine, 10, 174-184. DOI Scopus68 WoS52 Europe PMC57
2015	Jia, Y., Wong, D., Sweetman, C., Bruning, J., & Ford, C. (2015). New insights into the evolutionary history of plant sorbitol dehydrogenase. BMC Plant Biology, 15(1), 101-1-101-23. DOI Scopus32 WoS21 Europe PMC19
2015	Kroker, A., & Bruning, J. (2015). p21 exploits residue Tyr151 as a tether for high-affinity PCNA binding. Biochemistry, 54(22), 3483-3493. DOI Scopus27 WoS23 Europe PMC18
2015	Coleman, T., Chao, R., Bruning, J., De Voss, J., & Bell, S. (2015). CYP199A4 catalyses the efficient demethylation and demethenylation of para-substituted benzoic acid derivatives. RSC Advances, 5(64), 52007-52018. DOI Scopus33 WoS25
2015	Marciano, D., Kuruvilla, D., Boregowda, S., Asteian, A., Hughes, T., Garcia-Ordonez, R., . . . Griffin, P. (2015). Pharmacological repression of PPARγ promotes osteogenesis. Nature Communications, 6(1), 7443-1-7443-7. DOI Scopus102 WoS84 Europe PMC81
2015	Kojetin, D., Matta-Camacho, E., Hughes, T., Srinivasan, S., Nwachukwu, J., Cavett, V., . . . Nettles, K. (2015). Structural mechanism for signal transduction in RXR nuclear receptor heterodimers. Nature Communications, 6(1), 8013-1-8013-14. DOI Scopus104 WoS87 Europe PMC79
2015	Kroker, A., & Bruning, J. (2015). Review of the structural and dynamic mechanisms of PPAR γ partial agonism. PPAR Research, 2015, 816856-1-816856-15. DOI Scopus166 WoS104 Europe PMC110
2014	Chua, K., Pietsch, M., Zhang, X., Hautmann, S., Chan, H., Bruning, J., . . . Abell, A. (2014). Macrocyclic protease inhibitors with reduced peptide character. Angewandte Chemie International Edition, 53(30), 7828-7831. DOI Scopus23 WoS20 Europe PMC11
2014	Sullivan, A., Raimondo, A., Schwab, T., Bruning, J., Froguel, P., Farooqi, I., . . . Whitelaw, M. (2014). Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2. Biochemical Journal, 461(3), 403-412. DOI Scopus9 WoS8 Europe PMC9
2014	Bersten, D., Bruning, J., Peet, D., & Whitelaw, M. (2014). Human variants in the Neuronal Basic Helix-Loop-Helix/ Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. PLoS One, 9(1), e85768-1-e85768-11. DOI Scopus20 WoS20 Europe PMC21
2014	Reddy, M., Breda, A., Bruning, J., Sherekar, M., Valluru, S., Thurman, C., . . . Sacchettini, J. (2014). Structure, activity, and inhibition of the carboxyltransferase β-Subunit of acetyl coenzyme a carboxylase (AccD6) from mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 58(10), 6122-6132. DOI Scopus20 WoS16 Europe PMC13
2013	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2013). Redefining the role of the quaternary shift in bacillus stearothermophilus phosphofructokinase. Biochemistry, 52(32), 5421-5429. DOI Scopus8 WoS4 Europe PMC8
2013	Bonnefond, A., Raimondo, A., Stutzmann, F., Ghoussaini, M., Ramachandrappa, S., Bersten, D., . . . Froguel, P. (2013). Loss-of-function mutations in SIM1 contribute to obesity and Prader-Willi-like features. Journal of Clinical Investigation, 123(7), 3037-3041. DOI Scopus106 WoS78 Europe PMC79
2013	Ramachandrappa, S., Raimondo, A., Cali, A., Keogh, J., Henning, E., Saeed, S., . . . Farooqi, I. (2013). Rare variants in single-minded 1 (SIM1) are associated with severe obesity. Journal of Clinical Investigation, 123(7), 3042-3050. DOI Scopus132 WoS98 Europe PMC101
2012	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2012). Structure of the apo form of Bacillus Stearothermophilus Phosphofructokinase. Biochemistry, 51(3), 769-775. DOI Scopus9 WoS8 Europe PMC6
2011	Bruning, J., Murillo, A., Chacon, O., Barletta, R., & Sacchettini, J. (2011). Structure of the Mycobacterium tuberculosis D-alanine:D-alanine Ligase, a target of the antituberculosis drug D-Cycloserine. Antimicrobial Agents and Chemotherapy, 55(1), 291-301. DOI Scopus128 WoS103 Europe PMC74
2011	Zhang, J., Chalmers, M., Stayrook, K., Burris, L., Wang, Y., Busby, S., . . . Griffin, P. (2011). DNA binding alters coactivator interaction surfaces of the intact VDR-RXR complex. Nature Structural and Molecular Biology, 18(5), 556-563. DOI Scopus170 WoS146 Europe PMC136
2011	Chim, N., Habel, J., Johnston, J., Krieger, I., Miallau, L., Sankaranarayanan, R., . . . Goulding, C. (2011). The TB structural genomics consortium: a decade of progress. Tuberculosis, 91(2), 155-172. DOI Scopus36 WoS32 Europe PMC30
2011	Choi, J., Banks, A., Kamenecka, T., Busby, S., Chalmers, M., Kumar, N., . . . Griffin, P. (2011). Anti-diabetic actions of a non-agonist PPARgamma ligand that blocks Cdk5-mediated phosphorylation. Nature, 477(7365), 477-481. DOI Scopus466 WoS381 Europe PMC356
2010	Bruning, J., Parent, A., Gil, G., Zhao, M., Nowak, J., Pace, M., . . . Nettles, K. (2010). Coupling of receptor conformation and ligand orientation determine graded activity. Nature Chemical Biology, 6(11), 837-843. DOI Scopus121 WoS109 Europe PMC85
2009	Reddy, M., Palaninathan, S., Bruning, J., Thurman, C., Smith, D., & Sacchettini, J. (2009). Structural insights into the mechanism of the allosteric transitions of Mycobacterium Tuberculosis cAMP receptor protein. Journal of Biological Chemistry, 284(52), 36581-36591. DOI Scopus37 WoS30 Europe PMC27
2008	Nettles, K., Bruning, J., Gil, G., Nowak, J., Sharma, S., Hahm, J., . . . Greene, G. (2008). NFκB selectivity of estrogen receptor ligands revealed by comparative crystallographic analyses. Nature Chemical Biology, 4(4), 241-247. DOI Scopus136 WoS127 Europe PMC103
2008	Dai, S., Chalmers, M., Bruning, J., Bramlett, K., Osborne, H., Montrose-Rafizadeh, C., . . . Griffin, P. (2008). Prediction of the tissue-specificity of selective estrogen receptor modulators by using a single biochemical method. Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7171-7176. DOI Scopus80 WoS67 Europe PMC58
2007	Nettles, K., Bruning, J., Gil, G., O'Neill, E., Nowak, J., Hughs, A., . . . Greene, G. (2007). Structural plasticity in the oestrogen receptor ligand-binding domain. EMBO Reports, 8(6), 563-568. DOI Scopus119 WoS109 Europe PMC89
2007	Zhou, H., Nettles, K., Bruning, J., Kim, Y., Joachimiak, A., Sharma, S., . . . Katzenellenbogen, J. (2007). Elemental isomerism: a boron-nitrogen surrogate for a carbon-carbon double bond increases the chemical diversity of estrogen receptor ligands. Chemistry & Biology, 14(6), 659-669. DOI Scopus63 WoS62 Europe PMC42
2007	Bruning, J., Chalmers, M., Prasad, S., Busby, S., Kamenecka, T., He, Y., . . . Griffin, P. (2007). Partial agonists activate PPARγ using a helix 12 independent mechanism. Structure, 15(10), 1258-1271. DOI Scopus326 WoS278 Europe PMC238
2004	Bruning, J. B., & Shamoo, Y. (2004). Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-δ p66 subunit and flap endonuclease-1. Structure, 12(12), 2209-2219. DOI Scopus180 WoS168 Europe PMC156
-	Coleman, T., Podgorski, M. N., Doyle, M. L., Scaffidi-Muta, J. M., Campbell, E. C., Bruning, J. B., . . . Bell, S. (n.d.). Cytochrome P450-Catalyzed Oxidation of Halogen-Containing Substrates. SSRN Electronic Journal. DOI

Book Chapters

Year	Citation
2021	Chua, B. S. K., & Bruning, J. B. (2021). PPARα and δ Ligand Design: Honing the Traditional Empirical Method with a More Holistic Overview. In M. Z. Badr (Ed.), Nuclear Receptors: The Art and Science of Modulator Design and Discovery (pp. 111-178). Cham, Switzerland: Springer. DOI
2019	Frkic, R., & Bruning, J. (2019). Obtaining crystals of PPARγ ligand binding domain bound to small molecules. In Unknown Book (Vol. 1966, pp. 253-260). DOI Scopus3 Europe PMC1

Conference Papers

Year	Citation
2023	Nguyen, S., Jovcevski, B., Truong, J. Q., Pukala, T. L., & Bruning, J. B. (2023). A structural model of the human plasminogen and <i>Aspergillus fumigatus</i> enolase complex: insights for antifungal drug discovery. In ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES Vol. 79 (pp. C706). INT UNION CRYSTALLOGRAPHY.
2010	Mosser, R., Reddy, M., Bruning, J., Sacchettini, J. C., & Reinhart, G. D. (2010). Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase. In BIOPHYSICAL JOURNAL Vol. 98 (pp. 39A). CELL PRESS. DOI
2007	Chalmers, M. J., Busby, S. A., Prasad, S., Bruning, J. B., Pascal, B. D., Southern, M. R., & Griffin, P. R. (2007). P97-S A High-Throughput Method for the characterization of Nuclear Receptor Dynamics. In J Biomol Tech. Vol. 18 (pp. 33). Tampa Florida.

Conference Items

Year	Citation
2020	Tavakoli, P., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent Activation of JAK/STAT Signaling Plays an Important Role in<i>in Vitro</i>Jaki Resistance in <i>TYK2</i>-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: AMER SOC HEMATOLOGY. DOI
2020	Tavakoli, P. S., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent activation of JAK/STAT signaling plays an important role in in vitro Jaki resistance in TYK2-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of 62nd Annual Meeting of the American Society of Hematology. San Diego, California (virtual conference).
2020	Downes, C. E. J., McClure, B. J., Rehn, J., Breen, J., Bruning, J. B., Yeung, D. T., & White, D. L. (2020). Acquired Mutations within the JAK2 Kinase Domain Confer Resistance to JAK Inhibitors in an <i>in Vitro</i> model of a High-Risk Acute Lymphoblastic Leukemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: AMER SOC HEMATOLOGY. DOI WoS1
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of APG Student Awards.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of ruxolitinib resistance mutations in Pro-B cells driven by a high-risk JAK2 fusion in B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of ASMR.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and Characterisation of Ruxolitinib Resistant Mutations in JAK2-rearranged B-cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of AGTA.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of mutations that cause ruxolitinib resistance in Pro-B cells driven by a high-risk B-ALL JAK2-fusion.. Poster session presented at the meeting of ANZCHOG.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of SAHMRI Scientific Seminar.
2018	Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of EMBL Australia Postgraduate Symposium.

Original Creative Works

Year	Citation
2018	Authors: Wegener K, Mcgrath A, Dixon N, Oakley A, Scanlon D, Abell A, Bruning J. Title: Rational Design of a 310-Helical PIP-Box Mimetic Targeting PCNA, the Human Sliding Clamp [Cover Profile]. Description: N/A. Extent: N/A. DOI

Preprint

Year	Citation
2025	McDougal, D., Pederick, J., Shearwin-Whyatt, L., Grützner, F., & Bruning, J. (2025). Structural evolution of the estrogen receptor regulatory domain. DOI Europe PMC1
2025	McDougal, D., Pederick, J., Novick, S., Jovcevski, B., Warrender, A., Pascal, B., . . . Bruning, J. (2025). A ternary switch determines ERα LBD conformation. DOI
2023	Lee, J., Bruning, J., & Bell, S. (2023). In Crystallo Reactions with an Engineered Cytochrome P450 Peroxygenase. DOI

ARC DP (2024). DP240101500. A/Prof Stephen Bell ; Prof James De Voss ; A/Prof John Bruning. New biocatalysts for selective chemical oxidations under extreme conditions ($781,608).
Oracle Research Grant (2023). A/P John Bruning. High throughput protein folding for in-silico characterisation of designed and natural protein.
ARC DP (2023) DP230100609. A/Prof John Bruning ; Prof Frank Gruetzner ; Prof Patrick Griffin. Structural and molecular studies of endocrine disruption in Australia fauna ($563,171).
NHMRC Idea Grant (2021). Dr Nichoklas Eyre; Dr Amanda Aloia, Dr Thomas Avery; Dr John Bruning, Dr Tim Chataway. Development and mechanistic analysis of novel inhibitors of the dengue virus NS1 protein ($783,440)
ARC ITTC (2019). IC190100017. Prof Peter Dowd ; Prof William Skinner ; Prof Frank Neumann ; Prof Stephen Grano ; A/Prof Damith Ranasinghe ; Dr Tien-Fu Lu ; Prof Anton Kepic ; A/Prof Chaoshui Xu ; Prof Nigel Cook ; A/Prof Jixue Liu ; Prof David Lancaster .... Dr John Bruning. ARC Training Centre for Integrated Operations for Complex Resources ($3,703,664)
ARC LIEF Grant (2014). LE15010023. Michael, Dr. Micahel Z; Booker, A/Prof., Grant W; Venter, Dr. Henrietta; Pitson, Prof. Stuart M; McKinnon, Prof. Ross A; Abell, Prof. Andrew D; Soole, A/Prof. Kathleen L; Sykes, Prof. Pamela J; Bruning, Dr. John B. “High Throughput Screening Facility.” ($860,000).
Environment Institute Major Collaborative Project (2015). Dr. Frank Reith, Prof. Peter Hoffmann, Dr. Florian Weiland, Dr. Benjamin Wade, Dr. John Bruning. ($50,000).
Biochemistry Discipline Collaborative Research Grant (2015-2018). Bruning, JB and Booker, GW. “Structural studies of Aspergillus fumigatus enzyme targets for drug discovery.” ($45,000).
AIB Labs, Research Infrastructure Fund (2015). Parkinson-Lawrence, E., Polyak, S.W., Brooks, D., Booker, G.W., Forbes, B.E., Pitson, S., Cowin, A., Bruning, J., Butler, L. ($12,793).
IPAS Pilot Projects (2015). Krogsgaard-Larsen, N.; Bruning, J.B.; Luiten, A.; Callen, D.; Zhang, X.Z.; Alaknanda, A. “First Steps toward a Cancer Biosensor.” ($6,000).
Australian Synchrotron CAP (2013). CAP6919. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”
Australian Synchrotron CAP (2014). CAP8221. Hrmova, M. and Bruning, J.B. “X-ray crystal structures of plant and mammalian proteins, and protein design.”

BIOCHEM3000 – Molecular and Structural Biology
BIOCHEM3230 – Molecular and Structural Biology (Biomedical Science)
BIOCHEM3125 – Advanced Molecular Biology IIIA (Biochemistry)
BIOCHEM3001 – Cancer, Stem Cells and Development III
BIOCHEM3235 – Cancer, Stem Cells and Development III (Biomedical Science)
BIOCHEM3520 – Cancer, Stem Cells and Development (Theory) III
BIOCHEM3225 – Advanced Molecular Biology IIIB (Biochemistry)
BIOCHEM2500 – Biochemistry II: Molecular & Cell Biology
BIOCHEM2502 – Biochemistry II (Biotech): Molecular & Cell Biology
BIOCHEM2504 – Biochemistry II (Mol Biol): Molecular & Cell Biology
BIOLOGY1201 – Biology I – Human Perspectives
CHEMENG 2015 - Principles of Biotechnology II
BIOTECH7000 – Advanced Research Platforms
BIOTECH7001 – Drug Discovery and Development
Science2300 – Principles and Practice of Research

Current Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2025	Co-Supervisor	Exploring the molecular interplay of highly related tumour suppressor proteins that bind identical DNA motifs	Master of Philosophy (Medical Science)	Master	Full Time	Miss Katarina Anastasia Amerl
2025	Co-Supervisor	Characterising Endocrine Disrupting Chemicals and Synthetic Agonists as Peroxisome Proliferator Activated Receptor Ligands.	Doctor of Philosophy	Doctorate	Full Time	Mr Samuel Luke Wallis
2025	Co-Supervisor	Investigating the role of TLR4 antagonists and the effects on chronic pain: a structural biology journey	Doctor of Philosophy under a Jointly-awarded Degree Agreement with	Doctorate	Full Time	Mr Anston Savio D'silva
2024	Co-Supervisor	Characterization of peroxygenase activity in CYP154 enzymes from the thermophilic bacterium Thermobifida fusca	Doctor of Philosophy	Doctorate	Full Time	Ms Oghenesivwe Osiebe
2024	Principal Supervisor	The design of novel peptide mimetics or small molecule inhibitors of proliferating cell nuclear antigen (PCNA) for the development of broad-spectrum cancer therapeutics.	Doctor of Philosophy	Doctorate	Full Time	Miss Dayna Louise Holroyd
2024	Co-Supervisor	Innate phagocytosis from a basic feeding behaviour to a regulated defence action	Doctor of Philosophy	Doctorate	Full Time	Mr Wei Da Ho
2024	Principal Supervisor	Selective Androgen Receptor Modulators (SARMs) for the Treatment of Androgen Receptor positive (AR+) and Estrogen Receptor Positive (ER+) Breast Cancer.	Doctor of Philosophy	Doctorate	Full Time	Mr Manjit Rana
2024	Co-Supervisor	Exploration and Exploitation of Novel Thermally Adapted Cytochromes P450 and their Biotechnological Uses	Doctor of Philosophy	Doctorate	Full Time	Mr Delroy Sheng Yu Huang
2024	Co-Supervisor	The Study of Cholesterol Oxidizing Enzymes from Actinobacteria	Doctor of Philosophy	Doctorate	Full Time	Dr Gashaw Getaneh Dagnaw
2023	Co-Supervisor	Investigation of transcriptional regulators as a novel approach to treat ovarian cancer	Doctor of Philosophy	Doctorate	Full Time	Ms Suet Yee Too
2022	Co-Supervisor	Researching the Interaction, Structure and Function of S-Proteins in Almond	Doctor of Philosophy under a Jointly-awarded Degree Agreement with	Doctorate	Full Time	Ms Isis Pinner
2021	Principal Supervisor	Development and Analysis of Gold Sensing Protein	Doctor of Philosophy	Doctorate	Full Time	Mr Akhil Kumar

Past Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2020 - 2024	Principal Supervisor	Insights into Peptidomimetic Approaches for Inhibiting the DNA Sliding Clamps	Doctor of Philosophy	Doctorate	Full Time	Miss Bethiney Chantel Vandborg
2020 - 2023	Co-Supervisor	Development of 2-Aminoquinoline Derivatives with Improved Drug-Like Character as Small Molecule Inhibitors for a Protein-Protein Interaction Domain	Doctor of Philosophy	Doctorate	Part Time	Ms Ellen Lillian Swan
2019 - 2021	Co-Supervisor	Targeting PCNA with Cell and Nuclear Permeable p21- derived Peptides	Master of Philosophy	Master	Full Time	Miss Theresa Chav
2019 - 2023	Principal Supervisor	The structural selectivity and various ligands of PPAR gamma	Doctor of Philosophy	Doctorate	Full Time	Mr Benjamin Soon Kai Chua
2019 - 2025	Principal Supervisor	A Structural Analysis of Estrogen Receptor Evolution and Allosteric Regulation	Doctor of Philosophy	Doctorate	Full Time	Mr Daniel Patrick McDougal
2018 - 2021	Principal Supervisor	Structural and Biochemical Insights into Antifungal Drug Targets from Aspergillus fumigatus	Doctor of Philosophy	Doctorate	Full Time	Ms Stephanie Nguyen
2018 - 2023	Principal Supervisor	Functional and Structural Characterisation of Bacterial ATP-grasp Ligases	Doctor of Philosophy	Doctorate	Full Time	Mr Jordan Luke Pederick
2018 - 2022	Co-Supervisor	Precision Medicine Approaches for JAK2-Rearranged Acute Lymphoblastic Leukaemia: The Efficacy of Targeted Therapies and Molecular Mechanisms of Drug Resistance	Doctor of Philosophy	Doctorate	Full Time	Miss Charlotte Emma-Jeane Downes
2018 - 2021	Co-Supervisor	Approaches to study protein interactions	Doctor of Philosophy	Doctorate	Full Time	Miss Aimee Jade Horsfall
2018 - 2019	Co-Supervisor	Structural and Biophysical Characterisation of Mycobacterium tuberculosis Dethiobiotin Synthetase	Doctor of Philosophy	Doctorate	Full Time	Andrew Philip Thompson
2018 - 2023	Co-Supervisor	Structural and Functional Investigations of Cytochrome P450 Enzymes from Mycobacterium Species	Doctor of Philosophy	Doctorate	Full Time	Miss Hebatalla Ahmed Ibrahim Mohamed
2017 - 2021	Principal Supervisor	Understanding PPARg-Ligand Interactions to Treat Type 2 Diabetes	Doctor of Philosophy	Doctorate	Full Time	Miss Rebecca Louise Frkic
2017 - 2019	Co-Supervisor	Microbial Biosynthesis of Grapevine Specialised Metabolites	Master of Philosophy	Master	Full Time	Miss Emi Kaye Schutz
2016 - 2017	Principal Supervisor	Defining the Key Residues in PCNA-Protein Interactions	Master of Philosophy	Master	Part Time	Alice Jane Kroker
2016 - 2021	Co-Supervisor	Eukaryotic elongation factor 2 kinase (eEF2K): its relation to cancer cell survival, autophagy and other cell signalling pathways	Doctor of Philosophy	Doctorate	Full Time	Mr Roman Valeryevich Lenchine
2015 - 2021	Co-Supervisor	Structural Characterization of Lysogeny-Promoting Transcription Factor of Bacteriophage 186	Doctor of Philosophy	Doctorate	Full Time	Mr Jia Quyen Truong
2014 - 2018	Principal Supervisor	Structural and Biochemical Studies on Three Aspergillus fumigatus Proteins That Present as Targets for Novel Antifungal Drugs	Doctor of Philosophy	Doctorate	Full Time	Andrew Craig Marshall
2014 - 2016	Co-Supervisor	Molecular and Structural Characterization of the Candidate Enzymes Responsible for Tartaric Acid Synthesis in the Grapevine	Doctor of Philosophy	Doctorate	Full Time	Mr Yong Jia

Position: Senior Lecturer
Phone: 83135218
Email: john.bruning@adelaide.edu.au
Fax: 83134362
Campus: North Terrace
Building: Molecular Life Sciences, floor 2
Org Unit: Molecular and Biomedical Science