Dr Iain Searle
Deputy Head of Genetics and Evolution
School of Biological Sciences
Faculty of Sciences
Eligible to supervise Masters and PhD - email supervisor to discuss availability.
- My Research
- Career
- Publications
- Grants and Funding
- Teaching
- Supervision
- Professional Activities
- Contact
Our research in plant and animal epigenetics has been driven by a strong interest in understanding and elucidating the molecular mechanisms of how plants and animals adapt to environmental signalling and how speciation barriers evolve in plants. We use a combination of next-generation sequencing, bioinformatics and synthetic biology to describe and elucidate the function of these components.
Epigenetics, broadly defined, refers to all genetic information not encoded in the DNA sequence, with the best-understood consequence of epigenetic modifications being the regulation of gene expression. Although gene transcription and protein synthesis is required for plant development, the full repertoire of epigenetic mechanisms underpinning biological adaptation and speciation barriers remain equivocal.
The majority of the genome is pervasively transcribed such that more than 90% of transcriptional activity is related to the production of various long non-coding RNAs (lncRNA), which possess no or limited protein-coding capacity. Expansion of these transcriptionally active non-coding sequences in plant and animal genomes appears to have occurred primarily in higher multicellular organisms. We have identified thousands of novel lncRNAs transcribed in plants.
Furthermore, we have demonstrated RNA modifications play an important role in extending the transcriptional space. Thus, both RNA modifications and lncRNAs represent attractive candidates for achieving context- and stimulus-specific epigenetic regulation of gene expression.
Fundamental Research- We currently use a combination of molecular genetics, transcriptomics and bioinformatics (high-performance computing and synthetic biology) to answer our research questions on long non-coding RNAs and RNA modifications.
Honours, Masters, PhD and postdoctoral projects in genetics, epigenetics and bioinformatics are available.
Translational Research- We recently translated our wide-hybridisation technology to the Australian canola (Brassica napus) industry. In doing so we introduced disease resistance genes from either B. rapa or B. oleracea to synthesise B. napus (canola) with novel disease resistance. We are currently extending our translational canola research by connecting with international partners. Honours, Masters and PhD projects are available to develop the technology further in collaboration with industry partners. Are you a potential industry partner and want to connect? Don't hesitate to contact me.
Interested in joining our laboratory as an undergraduate placement student or postgraduate student? What to join our mentor programme? Ready to excel and be challenged with fascinating biological questions? Please don't hesitate to email me.
Publications- https://scholar.google.com.au/citations?hl=en&user=F23tKLkAAAAJ&view_op=list_works&sortby=pubdate
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Appointments
Date Position Institution name 2018 Senior Lecturer University of Adelaide 2014 - 2018 ARC Future Fellow University of Adelaide 2009 - 2014 ARC QEII Fellow Australian National University -
Language Competencies
Language Competency German Can speak and understand spoken -
Education
Date Institution name Country Title 2004 University of Queensland Australia PhD 1996 University of Queensland Australia B Ag Sci Hons -
Postgraduate Training
Date Title Institution Country 2007 - 2009 Wellcome Trust VIP Fellow University of Cambridge United Kingdom 2006 - 2007 Marie Curie Postdoctoral Fellow The Sainsbury Laboratory United Kingdom 2002 - 2006 Max Planck Postdoctoral Fellow Max Planck Institute for Plant Breeding Research Germany
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Journals
Year Citation 2020 Sinha, P., Singh, V., Saxena, R., Kale, S., Li, Y., Garg, V., . . . Varshney, R. (2020). Genome-wide analysis of epigenetic and transcriptional changes associated with heterosis in pigeonpea. Plant Biotechnology Journal, 14 pages.
2020 Nguyen, V., & Searle, I. R. (2020). An efficient root transformation system for recalcitrant Vicia sativa.. bioRxiv.
2020 Xi, H., Ryder, M., & Searle, I. R. (2020). The Complete Genome Sequence of Allorhizobium vitis Strain K306, the Causal Agent of Grapevine Crown Gall. Microbiologial Resource Annoucements. 2020 Nguyen, V., Riley, S., Nagel, S., Fisk, I., & Searle, I. R. (2020). Common Vetch: a drought tolerant, high protein neglected leguminous crop with potential as a sustainable food source.. Frontiers in Plant Science. 2020 Xiao, L., Jiang, S., Huang, P., Chen, F., Wang, X., Cheng, Z., . . . Zhang, X. -M. (2020). Two nucleoporin98-homologous genes jointly participate in the regulation of starch degradation to repress senescence in Arabidopsis.. BMC Plant Biology. 2019 Lim, S. M., Page, A. J., Li, H., Carragher, J., Searle, I., Robertson, S., & Muhlhausler, B. (2019). Sexually Dimorphic Response of Increasing Dietary Intake of High Amylose Wheat on Metabolic and Reproductive Outcomes in Male and Female Mice.. Nutrients, 12(1), 14 pages.
2019 Guo, Q., Ng, P. Q., Shi, S., Fan, D., Li, J., Zhao, J., . . . Searle, I. (2019). Arabidopsis TRM5 encodes a nuclear-localised bifunctional tRNA guanine and inosine-N1-methyltransferase that is important for growth. PLoS ONE, 14(11), e0225064-1-e0225064-26.
2019 David, R., Kortschak, R. D., & Searle, I. (2019). The root hair defective phenotype of Arabidopsis thaliana Pol IV subunit mutant nrpd1a-3 is associated with a deletion in RHD6. microPublication Biology, 1-4.
2018 Xu, K., Zhang, X. -M., Yu, G., Lu, M., Liu, C., Chen, H., . . . Fu, Y. -F. (2018). FT/FD-GRF5 repression loop directs growth to increase soybean yield. BioRvx, 1(1), 1-15.
2018 Wang, D., Gu, J., David, R., Wang, Z., Yang, S., Searle, I., . . . Timmis, J. (2018). Experimental reconstruction of double-stranded break repair-mediated plastid DNA insertion into the tobacco nucleus. The Plant Journal, 93(2), 227-234.
Scopus2 WoS2 Europe PMC12017 Crisp, P., Ganguly, D., Smith, A., Murray, K., Estavillo, G., Searle, I., . . . Pogson, B. (2017). Rapid recovery gene downregulation during excess-light stress and recovery in Arabidopsis. The Plant Cell, 29(8), 1836-1863.
Scopus21 WoS21 Europe PMC132017 Liu, L., Jiang, Y., Zhang, X., Wang, X., Wang, Y., Han, Y., . . . Chen, F. (2017). Two SUMO proteases SUMO PROTEASE RELATED TO FERTILITY1 and 2 are required for fertility in arabidopsis. Plant Physiology, 175(4), 1703-1719.
Scopus13 WoS12 Europe PMC82017 David, R., Burgess, A., Parker, B., Li, J., Pulsford, K., Sibbritt, T., . . . Searle, I. (2017). Transcriptome-wide Mapping of RNA 5-Methylcytosine in Arabidopsis mRNAs and non-coding RNAs. The Plant cell, 29(3), 16.00751-1-16.0075-30.
Scopus47 WoS41 Europe PMC212017 Wang, D., Qu, Z., Yang, L., Zhang, Q., Liu, Z., Do, T., . . . Zhu, J. (2017). Transposable elements (TEs) contribute to stress-related long intergenic noncoding RNAs in plants. Plant Journal, 90(1), 133-146.
Scopus39 WoS38 Europe PMC212016 Ashby, R., Forêt, S., Searle, I., & Maleszka, R. (2016). MicroRNAs in Honey Bee Caste Determination. Scientific Reports, 6(1), 15 pages.
Scopus43 WoS42 Europe PMC232016 Burgess, A., David, R., & Searle, I. (2016). Deciphering the epitranscriptome: a green perspective. Journal of Integrative Plant Biology, 58(10), 822-835.
Scopus17 WoS18 Europe PMC92016 Chen, M., Xu, J., Devis, D., Shi, J., Ren, K., Searle, I., & Zhang, D. (2016). Origin and functional prediction of pollen allergens in plants. Plant Physiology, 172(1), 341-357.
Scopus8 WoS6 Europe PMC32015 Liu, H., Searle, I., Watson-Haigh, N., Baumann, U., Mather, D., Able, A., & Able, J. (2015). Genome-wide identification of microRNAs in leaves and the developing head of four durum genotypes during water deficit stress. PLoS One, 10(11), e0142799-1-e0142799-30.
Scopus27 WoS26 Europe PMC162015 Burgess, A., David, R., & Searle, I. (2015). Conservation of tRNA and rRNA 5-methylcytosine in the kingdom Plantae. BMC Plant Biology, 15(1), 199-1-199-17.
Scopus40 WoS39 Europe PMC212015 Liu, H., Searle, I., Mather, D., Able, A., & Able, J. (2015). Morphological, physiological and yield responses of durum wheat to pre-anthesis water-deficit stress are genotype-dependent. Crop and Pasture Science, 66(10), 1024-1038.
Scopus18 WoS152013 Cazzonelli, C., Vanstraelen, M., Simon, S., Yin, K., Carron-Arthur, A., Nisar, N., . . . Pogson, B. (2013). Role of the Arabidopsis PIN6 auxin transporter in auxin homeostasis and auxin-mediated development. PLoS One, 8(7), 1-14.
Scopus33 WoS31 Europe PMC202012 Campoli, C., Drosse, B., Searle, I., Coupland, G., & von Korff, M. (2012). Functional characterisation of HvCO1, the barley (Hordeum vulgare) flowering time ortholog of CONSTANS. The Plant Journal, 69(5), 868-880.
Scopus91 WoS83 Europe PMC582011 Gursanscky, N., Searle, I., & Carroll, B. (2011). Mobile microRNAs hit the target. Traffic, 12(11), 1475-1482.
Scopus10 WoS10 Europe PMC52011 Burgess, A., & Searle, I. (2011). The clock primes defense at dawn. Immunology and Cell Biology, 89(6), 661-662.
Scopus4 WoS4 Europe PMC32011 Indrasumunar, A., Searle, I., Lin, M., Kereszt, A., Men, A., Carroll, B., & Gresshoff, P. (2011). Nodulation factor receptor kinase 1α controls nodule organ number in soybean (Glycine max L. Merr). The Plant Journal, 65(1), 39-50.
Scopus69 WoS67 Europe PMC492011 Castro Marín, I., Loef, I., Bartetzko, L., Searle, I., Coupland, G., Stitt, M., & Osuna, D. (2011). Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways. Planta, 233(3), 539-552.
Scopus82 WoS77 Europe PMC482010 Searle, I., Pontes, O., Melnyk, C., Smith, L., & Baulcombe, D. (2010). JMJ14, a JmjC domain protein, is required for RNA silencing and cell-to-cell movement of an RNA silencing signal in Arabidopsis. Genes and Development, 24(10), 986-991.
Scopus72 WoS70 Europe PMC542010 Indrasumunar, A., Kereszt, A., Searle, I., Miyagi, M., Li, D., Nguyen, C., . . . Gresshoff, P. (2010). Inactivation of duplicated nod factor receptor 5 (NFR5) genes in recessive loss-of-function non-nodulation mutants of allotetraploid soybean (Glycine max L. Merr.). Plant and Cell Physiology, 51(2), 201-214.
Scopus72 WoS71 Europe PMC472009 Huang, L., Jones, A., Searle, I., Patel, K., Vogler, H., Hubner, N., & Baulcombe, D. (2009). An atypical RNA polymerase involved in RNA silencing shares small subunits with RNA polymerase II. Nature Structural and Molecular Biology, 16(1), 91-93.
Scopus99 WoS89 Europe PMC802007 Corbesier, L., Vincent, C., Jang, S., Fornara, F., Fan, Q., Searle, I., . . . Coupland, G. (2007). FT protein movement contributes to long-distance signaling in floral induction of Arabidopsis. Science, 316(5827), 1030-1033.
Scopus1222 WoS1171 Europe PMC8202007 Smith, L., Pontes, O., Searle, I., Yelina, N., Yousafzai, F., Herr, A., . . . Baulcombe, D. (2007). An SNF2 protein associated with nuclear RNA silencing and the spread of a silencing signal between cells in Arabidopsis. Plant Cell, 19(5), 1507-1521.
Scopus188 WoS179 Europe PMC1582006 Searle, I., He, Y., Turck, F., Vincent, C., Fornara, F., Kröber, S., . . . Coupland, G. (2006). The transcription factor FLC confers a flowering response to vernalization by repressing meristem competence and systemic signaling in Arabidopsis. Genes and Development, 20(7), 898-912.
Scopus487 WoS467 Europe PMC3472006 Corbesier, L., Vincent, C., Searle, I., Fomara, F., & Coupland, G. (2006). Analysis of the expression pattern of FT protein during flowering. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY, 143(4), S166. 2005 Berendzen, K., Searle, I., Ravenscroft, D., Koncz, C., Batschauer, A., Coupland, G., . . . Ülker, B. (2005). A rapid and versatile combined DNA/RNA extraction protocol and its application to the analysis of a novel DNA marker set polymorphic between Arabidopsis thaliana ecotypes Col-0 and Landsberg erecta. Plant Methods, 1(1), 15 pages.
Scopus49 WoS47 Europe PMC272004 Searle, I., & Coupland, G. (2004). Induction of flowering by seasonal changes in photoperiod. EMBO Journal, 23(6), 1217-1222.
Scopus192 WoS179 Europe PMC1182003 Searle, I., Men, A., Laniya, T., Buzas, D., Iturbe-Ormaetxe, I., Carroll, B., & Gresshoff, P. (2003). Long-distance signaling in nodulation directed by a CLAVATA1-like receptor kinase. Science, 299(5603), 109-112.
Scopus349 WoS337 Europe PMC2332003 Waldron, J., Peace, C., Searle, I., Furtado, A., Wade, N., Findlay, I., . . . Carroll, B. (2003). Randomly Amplified DNA Fingerprinting: A culmination of DNA marker technologies based on arbitrarily-primed PCR amplification. Journal of Biomedicine and Biotechnology, 2(3), 141-150.
Scopus21 Europe PMC102002 May, K., Whisson, S., Zwart, R., Searle, I., Irwin, J., Maclean, D., . . . Drenth, A. (2002). Inheritance and mapping of 11 avirulence genes in Phytophthora sojae. Fungal Genetics and Biology, 37(1), 1-12.
Scopus50 WoS42 Europe PMC352002 Waldron, J., Peace, C., Searle, I., Furtado, A., Wade, N., Findlay, I., . . . Carroll, B. (2002). Randomly amplified DNA fingerprinting: A culmination of DNA marker technologies based on arbitrarily-primed PCR amplification. Journal of Biomedicine and Biotechnology, 2002(3), 141-150.
Scopus171999 Schenk, G., Ge, Y., Carrington, L., Wynne, C., Searle, I., Carroll, B., . . . De Jersey, J. (1999). Binuclear metal centers in plant purple acid phosphatases: Fe-Zn in sweet potato and Fe-Zn in soybean. Archives of Biochemistry and Biophysics, 370(2), 183-189.
Scopus124 WoS119 Europe PMC69 -
Book Chapters
Year Citation 2019 Do, T., Qu, Z., & Searle, I. (2019). Purification and functional analysis of plant long noncoding RNAs (lncRNA). In J. Chekanova, & H. -L. Wang (Eds.), Plant long non-coding RNAs: methods and protocols (Vol. 1933, pp. 131-147). New York: Springer.
2018 Li, J., Wu, X., Do, T., Nguyen, V., Zhao, J., QinNg, P., . . . Searle, I. R. (2018). Quantitative and single nucleotide resolution profiling of RNA 5-methylcytosine.. In R. Gupta, & N. Wajapeyee (Eds.), Epitranscriptomics: Methods and Protocols. -
Conference Papers
Year Citation 2017 Yang, S. Y., Tate, M., Asenstorfer, R., Carragher, J., Paull, J., & Searle, I. (2017). Progress Towards Developing Low Toxin Common Vetch (Vicia sativa L.). In BIT’s 5th World Congress of Agriculture-2017. Shenyang, China. 2007 Searle, I., Mosher, R., Melnyk, C., & Baulcombe, D. (2007). Small RNAs hit the big time: Meetings. In New Phytologist Vol. 174 (pp. 479-482). England: WILEY.
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Conference Items
Year Citation 2015 David, R., Lim, H. M., & Searle, I. R. (2015). Identification of pla/ubp14, an Arabidopsis mutant that displays lengthened plastochron and larger organs. Poster session presented at the meeting of Annual Genetics Society of Australasia conference. -
Theses
Year Citation 2016 Liu, H. (2016). Genome-wide characterisation of microRNAs and their target genes in different durum wheat genotypes under water limiting conditions. (PhD Thesis, University of Adelaide). -
Datasets
Year Citation 2015 Liu, H., Searle, I., Watson-Haigh., Baumann., Mather, D. E., Able, A. J., & Able, J. A. (2015). Genome-wide identification of differentially expressed microRNAs in leaves and the developing head of four durum genotypes during water deficit stress.
Competitive funding only listed below
- SAGIT grant- Investigators Searle and Nagel (2020-2023)
- ARC Discovery Grant DP190101303- Investigators Searle and Studholme (2019-2021)
- Hermon Slade Foundation- Investigator Searle (2017-2020)
- ACSRF Australia-China Joint Research Centre of Grains for Health- Investigators, Searle, et al (2016-2019)
- ARC Future Fellowship FT130100525- Investigator Searle (2014-2018)
- ASTE Travel Grant- Investigator Searle (2015)
- ACSRF Australia-China Young Scientists Exchange Programme- Investigator Searle (2014)
- GRDC E-Innovation award- EzyCross- Investigators Searle & Salisbury (2013-2014)
- GRDC Travel Grant- Investigator Searle (2013)
- ANU Connect Ventures Direct Translation Fund- Investigators Lohe and Searle (2012)
- CSIRO SEIF top-up PhD scholarship- Investigators Searle and Helliwell (2011-2013)
- ARC Discovery Grant DP110103805- Investigators Searle and Helliwell (2011-2013)
- ARC QEII Fellowship DP0988846- Investigator Searle (2009-2014)
- Wellcome Trust Value-In-People Award- Investigator Searle (2008-2009)
- Epigenome travel scholarship- Investigator Searle (2007)
- Marie Curie International Incoming Fellowship- Investigator Searle (2006-2007)
- BIOTECH 7040- Coordinator for Applications of Next Generation Sequencing
- GENETICS 2510- Genetics IIA
- GENETICS 3211- Gene Expression & Human Developmental Genetics III
- SCIENCE 3500- Opening Doors to the Middle Kingdom
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Current Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2020 Principal Supervisor Application of Second and Third-Generation Sequencing to Vicia sativa Improvement Doctor of Philosophy Doctorate Full Time Mr Hangwei Xi 2019 Principal Supervisor The role of RNA modifications on RNA Processing in Arabidopsis thaliana Doctor of Philosophy Doctorate Full Time Miss Pei Qin Ng 2019 Principal Supervisor Vetch as a New Protein Source for the Human Diet Doctor of Philosophy under a Jointly-awarded Degree Agreement with Doctorate Full Time Mr Samuel Arthur George Riley 2018 Principal Supervisor The genetic basis of floral variation in the Brassicaceae Master of Philosophy Master Full Time Mrs Jayashrini Sakunthala Madawala 2018 Principal Supervisor The Role of RNA Modifications in Endosperm Development Doctor of Philosophy Doctorate Full Time Miss Huong Thi Thuy Ta 2017 Principal Supervisor Identification of an RNA m5C demethylose from plants Doctor of Philosophy Doctorate Full Time Miss Xingyu Wu 2017 Co-Supervisor Major Evolutionary Adaptive Events in Brassicaceous Plants and Their Insect Hosts Doctor of Philosophy Doctorate Full Time Mr Christopher Michael Ward 2017 Principal Supervisor Zero Toxin Grain Pulse Vetch Doctor of Philosophy Doctorate Full Time Miss Vy Hoang Thao Nguyen 2016 Principal Supervisor The Role of 5-Methly Cytosine during Plant Development Doctor of Philosophy Doctorate Full Time Miss Jing Zhao -
Past Higher Degree by Research Supervision (University of Adelaide)
Date Role Research Topic Program Degree Type Student Load Student Name 2016 - 2019 Co-Supervisor Don't Cry for Me: Evolutionary and Functional Analysis of Two Rice Pollen Allergens, Ory s 1 and Ory s 12 Doctor of Philosophy Doctorate Full Time Ms Deborah Devis 2016 - 2020 Principal Supervisor Identification and functional analysis of RNA modifications in Arabidopsis thaliana and Mus musculus Doctor of Philosophy Doctorate Full Time Miss Jun Li 2014 - 2018 Principal Supervisor Identification and Functional Characterization of Long Noncoding RNAs Involved in Endosperm Development of Arabidopsis thaliana Doctor of Philosophy Doctorate Full Time Mr Quang Trung Do 2013 - 2016 Co-Supervisor Genome-wide Characterisation of microRNAs and their target genes in different durum wheat genotypes under water limiting conditions Doctor of Philosophy Doctorate Full Time Dr Haipei Liu 2013 - 2016 Principal Supervisor Conservation and Function of RNA 5-methylcytosine in Plants Doctor of Philosophy Doctorate Full Time Miss Alice Louise Burgess
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Editorial Boards
Date Role Editorial Board Name Institution Country 2011 - ongoing Consulting Editor Frontiers in Genetics and Genomics — — -
Offices Held
Date Office Name Institution Country 2018 - ongoing Australian Epigenetic Alliance- South Australian Representative Australian Epigenetic Alliance (AEpiA) Australia 2016 - 2017 Deputy Head of the Department of Genetics and Evolution School of Biological Sciences, The University of Adelaide Australia 2015 - ongoing GSA South Australian Representative on Executive Genetics Society of AustralAsia Australia
Connect With Me
External Profiles
