Danny Wilson

Dr Danny Wilson


School of Biological Sciences

Faculty of Sciences

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

Single cell parasites such as malaria and Toxoplasma remain leading causes of disease in children and during pregnancy, with Australia and our nearest neighbours continuing to be impacted. Malaria alone causes >400,000 deaths per year, with children under five and pregnant women most at risk. The burden of Malaria falls on developing tropical and sub-tropical countries throughout the world, with the health and prosperity of Australia's nearest neighbours and major trading partners still heavily affected by these mosquito borne parasites. Up to a third of the worlds population is infected with food and water borne Toxoplasma parasites that can cause severe disease during pregnancy and for immunocompromised people, with the long-term impacts of infection not fully understood.
Our research applies multi-disciplinary approaches to better understand the unique biology of these parasites. We use this information to prioritise and develop vaccines that protect against infection and for the identification of new drug treatment strategies to cure people infected with these widespread disease causing parasites.

Half of the world’s population is at risk of infection with mosquito-borne malaria parasites of the genus Plasmodium. These complex eukaryotic parasites that live inside human cells cause widespread sickness and death throughout tropical and sub-tropical regions of the world including countries neighbouring Australia. The majority of the >400,000 malaria related deaths that occur each year are caused by the parasite Plasmodium falciparum in children under 5 years in Africa, Asia and South America.

Unfortunately, resistance has developed to our most effective anti-malarial drugs, resulting in poorer treatment outcomes for clinical cases of this deadly parasite. There is an urgent need to identify novel drugs to partner with current first-line treatments to fill this looming treatment gap. In addition, development of an effective vaccine would reduce the burden of disease and greatly facilitate efforts to eradicate malaria. Unfortunately, a highly effective vaccine to protect against malaria has not been developed to date and efforts to identify the best vaccine targets are ongoing.

My laboratory seeks to identify and characterise novel parasite proteins for their suitability as both drug and vaccine targets. We focus on invasion of the human red blood cell by the small invasive merozoite stage of the parasite lifecycle as a therapeutic target. By developing therapeutics (drugs and vaccines) that inhibit merozoite invasion of the red blood cell we can instantly disrupt the lifecycle of the parasite in the stage that causes all the symptoms of the disease. We apply a breakthrough P. falciparum merozoite purification method (PNAS, 2010) that is superior to alternatives for studying malaria parasite invasion biology, opening up new opportunities to study this previously intractable lifecycle stage (Figure 1).

Antimalarials that inhibit invasion

By applying state of the art methods, we aim to develop invasion inhibitory antimalarials as viable drugs in a climate of increasing resistance to frontline treatments. Using a highly reproducible flow cytometry approach (Antimicrobial Agents & Chemotherapy, 2013; Figure 2 A & B), we have screened traditional antimalarial therapies, protease inhibitors, kinase inhibitors, a range of antibiotics and identified several compounds and there analogues that rapidly inhibit merozoite invasion (BMC Biology, 2015). To understand how drug-like compounds target the essential process of merozoite invasion, we apply mutation analysis of gene targets, phenotypic screens of related Apicomplexan parasites, modifications and screens of analogues, fluorescence microscopy localisation and proteomic identification of drug binding targets. Importantly, many of the invasion inhibitory compounds identified in our research also kill at other stages of the parasites complex lifecycle, increasing there utility as broad acting antimalarials. Development of these invasion inhibitory compounds and identification of the mechanisms by which they inhibit invasion is the subject of ongoing research in the laboratory.

Functional characterisation of poorly defined merozoite vaccine targets

The function of nearly 50% of the genes in P. falciparum parasites is poorly described or not known altogether. Malaria proteins involved in invasion are no exception with a large number of potential vaccine targets having no known function. We apply gene-editing techniques to examine the potential role of some of these proteins in invasion and parasite growth. Using fluorescence microscopy, flow cytometry, genetic manipulation and proteomic techniques, we are characterising the localisation, interactions and functions of merozoite antigens with no known function to test their suitability as therapeutic targets.

Postgraduate and undergraduate opportunities

Honours, summer-student, masters and PhD projects are available to explore the biology of the malaria parasite and to develop new therapies against this deadly infectious organism. We are a small, supportive, research team that is applying new technologies to discover new ways of ending the burden of malaria disease. If you would like to find out more about our current research opportunities and whether our research is right for you, please contact Dr. Danny Wilson (danny.wilson@adelaide.edu.au). 

  • Appointments

    Date Position Institution name
    2017 - 2019 DVCR 3 Year Mid-career Beacon Fellowship University of Adelaide, Adelaide
    2015 Laboratory Head University of Adelaide
    2014 Burnet Institute Research Fellow (Honorary) Burnet Institute, Melbourne
    2014 - 2015 Senior Research Fellow (with Prof. James Paton) University of Adelaide
    2012 - 2015 Peter Doherty, Early Career Fellowship National Health and Medical Research Council, Canberra
    2011 - 2013 Senior Research Fellow (with Prof. Alan Cowman) Walter and Eliza Hall Institute of Medical Research, Melbourne
    2009 - 2011 Research Officer (with Prof. James Beeson) Walter and Eliza Hall Institute of Medical Research, Melbourne
  • Awards and Achievements

    Date Type Title Institution Name Country Amount
    2018 Research Award NHMRC Project Grant (CIA)- (773,613) National Health and Medical Research Council Australia
    2018 Research Award Channel 7 Research Foundation Channel 7 Childrens Research Foundation Australia 75,000
    2018 Research Award Universities Australia/DAAD Germany Joint Research Co-operation scheme Universities Australia/DAAD Australia 10,000
    2017 Fellowship DVCR 3 Year Mid-career Beacon Fellowship University of Adelaide
    2016 Research Award DVCR Research Infrastructure Scheme (CIA)- Advanced Confocal Microscope ($342,000) University of Adelaide Australia
    2016 Research Award DVCR Interdisciplinary Research Fund (CIA)- Imaris image analysis software ($48,211) University of Adelaide Australia
    2015 Research Award DVCR Interdisciplinary Research Fund (CID)- Metalloproteomics HPLC ($28,000) University of Adelaide Australia
    2014 Research Award NHMRC Project Grant (CIC)- ($607,484) Walter and Eliza Hall Institute Australia
    2012 Fellowship NHMRC Peter Doherty Early Career Fellowship National Health and Medical Research Institute
    2004 Scholarship NHMRC Dora Lush PhD Scholarship National Health and Medical Research Centre Australia
  • Education

    Date Institution name Country Title
    2004 - 2009 University of Melbourne, Melbourne Australia PhD
  • Research Interests

2020 - NHMRC Ideas Grant: APP2003712. CIA Dr. Danny Wilson. 

2020 - ARC LIEF (LE210100155). Advancing 4D fluorescence microscopy within Australia. CID and Management Team: Dr. Danny Wilson

2019 - Hospital Research Foundation, 3-Year Mid-career Fellowship. CIA: Dr. Danny Wilson

2019 - South Australian Sheep Industry Fund, Project Grant. CIB: Dr Danny Wilson

2019 - DAAD/Universities Australia-Germany Joint Research Co-operation Scheme (2020-2021). Lead-CI: Dr. Danny Wilson.

2017 - NHMRC Project Grant: APP1143974. CIA Dr. Danny Wilson. 

2017 - The University of Adelaide, Cross-Faculty Equipment Call. Oxford Nano-imager NIM 4 Microscope System. Lead CI: Dr. Danny Wilson

2017 - Channel 7 Children's Research Foundation Project GrantCIA: Dr. Danny Wilson

2017 - DAAD/Universities Australia-Germany Joint Research Co-operation Scheme (2018-2019). Lead-CI: Dr. Danny Wilson.

2016 - The University of Adelaide, DVCR, 3-Year Beacon Fellowship. CIA: Dr. Danny Wilson

2016 - The University of Adelaide, DVCR, Research Infrastructure Scheme. Advanced confocal microscope. CIA: Dr. Danny Wilson

2016 - The University of Adelaide, DVCR, Interdisciplinary Research Fund. Imaris microscopy analysis software. CIA: Dr. Danny Wilson

2015 - The University of Adelaide, DVCR, Interdisciplinary Research Fund. Metalloproteomics HPLC. CID: Dr. Danny Wilson

2013 - NHMRC Project Grant: APP1057960. CIC Dr. Danny Wilson. 

2011- NHMRC Peter Doherty Early Career Fellowship APP1035715. CIA Dr. Danny Wilson. 

2004- NHMRC Dora Lush PhD Scholarship 2004-2008: APP305553. CIA: Dr. Danny Wilson. 

  • 3rd Year: Infection and Immunity A, 2 lectures, Semester 1.
  • 2nd Year: Biomedical Science, 2 lectures, Semester 2.
  • 2nd Year: BSc Advance Mentor, Semester 1.
  • 1st Year: SGDE for human perspectives, Semester 2.
  • Current Higher Degree by Research Supervision (University of Adelaide)

    Date Role Research Topic Program Degree Type Student Load Student Name
    2021 Principal Supervisor Development of new drugs against malaria Doctor of Philosophy Doctorate Full Time Miss Juliana Oliveira de Souza
    2021 Co-Supervisor Development of methods to control toxoplasmosis and associated apicomplexan parasites in livestock Doctor of Philosophy Doctorate Full Time Mr Quinn Phillip Mackie
    2021 Principal Supervisor Insights into the Biological Constraints on Malaria Red Blood Cell Invasion and the Implications for Vaccine Development Doctor of Philosophy Doctorate Full Time Miss Jill Chmielewski
    2019 Principal Supervisor Functional characterisation of a putative Plasmodium falciparum invasion ligand and its homologues Doctor of Philosophy Doctorate Full Time Mr Juan Miguel Balbin
    2018 Principal Supervisor Characterisation of the Function and Vaccine Potential of Surface Exposed Antigens Required for Malaria Parasite Invasion of Human Host Cells Doctor of Philosophy Doctorate Full Time Ms Isabelle Grace Henshall
  • Past Higher Degree by Research Supervision (University of Adelaide)

    Date Role Research Topic Program Degree Type Student Load Student Name
    2017 - 2020 Principal Supervisor Functional Characterisation Of Novel Plasmodium falciparum Proteins And Their Role In Erythrocyte Invasion. Doctor of Philosophy Doctorate Full Time Mr Benjamin Scott Liffner
    2016 - 2020 Principal Supervisor Investigation of azithromycin analogues and proteasome-like inhibitors as quick-killing antimalarials Doctor of Philosophy Doctorate Full Time Miss Amy Lee Burns
  • Board Memberships

    Date Role Board name Institution name Country
    2016 - 2019 Representative Australian Society for Parasitology, SA Representative Australia
  • Committee Memberships

    Date Role Committee Institution Country
    2017 - ongoing Secretary Molecular Life Sciences Microscopy Committee University of Adelaide Australia
    2016 - 2018 Representative Australian Society for Parasitology, Education Committee Australian Society for Parasitology Australia
    2013 - 2013 Treasurer Malaria in Melbourne Organising Committee Australia
  • Memberships

    Date Role Membership Country
    2017 - ongoing Member Light Microscopy Australia Australia
    2014 - ongoing Member Adelaide Protein Group Australia
    2014 - ongoing Member Australian Society for Biochemistry and Molecular Biology Australia
    2013 - ongoing Representative Australian Society for Parasitology Australia
  • Position: Postdoctoral
  • Phone: 83130413
  • Email: danny.wilson@adelaide.edu.au
  • Fax: 8313 4362
  • Campus: North Terrace
  • Building: Molecular Life Sciences, floor 4
  • Room: 4 13
  • Org Unit: Molecular and Biomedical Science

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