Clifford Young

Dr Clifford Young

Research Fellow

School of Pharmacy and Biomedical Sciences

College of Health

Eligible to supervise Masters and PhD (as Co-Supervisor) - email supervisor to discuss availability.

Clifford is a Research Fellow in the School of Pharmacy and Biomedical Science.
 
He obtained a PhD in Biochemistry from Victoria University of Wellington after using gel-based proteomics to study the cellular effects of an algal toxin. His interests in proteomic technologies led the New Zealander to Denmark, where he spent time at the University of Southern Denmark and University of Copenhagen learning and implementing strategies to investigate the biological roles of protein post-translational modifications. These chemical additions are notoriously difficult to study because of their low abundance, which often require enrichment protocols and detection by high resolution mass spectrometry. Clifford also has extensive experience with liquid chromatography and Orbitrap mass spectrometers, as well as performing selected/parallel reaction monitoring for the quantification of peptides and metabolites.
 
Clifford initially joined the UniSA Future Industries Institute in 2018 before moving to UniSA Clinical & Health Sciences in 2021. He is enthusiastic about scientific collaborations with research groups and industrial companies in South Australia. Interested parties are welcome to contact Clifford for initial discussions.

Year Citation
2026 Donnellan, L., Young, C., Seidel, J. D., Peake, B., Lau, D., Deo, P., & Hoffmann, P. (2026). An Investigation into the Differentiation of Lactyllysine and Carboxyethyllysine Peptide Modifications by Liquid Chromatography/Mass Spectrometry. Journal of Proteome Research, 25(3), 1601-1610.
DOI
2026 Lokman, N. A., Macpherson, A. M., Thompson, A. R., Price, Z. K., Goonetilleke, L., Condina, M. R., . . . Ricciardelli, C. (2026). Proteomics analysis of serum extracellular vesicle identifies UCHL1 as a potential therapeutic target for high grade serous ovarian cancer. British Journal of Cancer, 134(2), 195-207.
DOI
2025 Desire, C. T., Dilmetz, B. A., Degenhardt, J., Donnellan, L., Condina, M. R., Young, C., & Hoffmann, P. (2025). The Importance of Denaturing Conditions for Protein and Peptide Quantification by Tryptophan Fluorescence. ACS Omega, 10(46), 56795-56805.
DOI
2025 Donnellan, L., Fenech, M., Dhillon, V. S., Young, C., Hoffmann, P., & Deo, P. (2025). Role of methylglyoxal protein modifications in DNA damage and chromosomal instability: Emerging molecular mechanisms. Mutation Research Reviews in Mutation Research, 796(108558), 10 pages.
DOI Scopus4 WoS4 Europe PMC4
2025 Jasni, N., Khairul, K. B., Kin, W. W., Lau, D., Young, C., Hoffmann, P., & Othman, N. (2025). In-silico, in-vitro, and proteomics analyses on repurposed drugs in targeting the small GTPase, Rho subfamily protein (Rho GTPase), and putative Rho GTPase-activating protein (RhoGAP) of Giardia lamblia. Journal of Genetic Engineering and Biotechnology, 23(4), 13 pages.
DOI
2025 Palethorpe, H. M., Shard, C., Scheer, K. G., Ryan, C. P., Fouladzadeh, A., Wenholz, D., . . . Gomez, G. A. (2025). An Optimized Explant Organoid Method for Preclinical Brain Tumor Research. NEURO-ONCOLOGY, 27(Supplement_5), v167.
DOI
2025 Pires, M. C., Jungbauer, A., Lau, D., Young, C., Hoffmann, P., de Pádua, T. F., & Gerstweiler, L. (2025). Column ageing of agarose-based chromatography in industrial lactoferrin purification. Journal of Chromatography A, 1760(466274), 466274.
DOI
2025 Seidel, J. D., Young, C., Klingler Hoffmann, M., Condina, M. R., Shah, A., Ramarathinam, S., . . . Hoffmann, P. (2025). Analytical methods for in-depth characterisation of cell culture bioreactors: A case study. Journal of Chromatography Open, 8(100248), 1-13.
DOI
2025 Ngai, Y. T., Young, C., Parkinson-Lawrence, E. J., Wimmer-Kleikamp, S., Mittal, P., Beard, H., . . . Hoffmann, P. (2025). Defining lung pathogenesis in a murine model of mucopolysaccharidosis Type I by proteomic analysis. Molecular Genetics and Metabolism, 146(1-2), 109231.
DOI
2025 Subramaniam, S., Donnellan, L., Wignall, A., Woodcock, J., Coolen, C., Pitson, S., . . . Joyce, P. (2025). Polymeric-lipid nanoparticles that leverage cationic helper lipids and the protein corona for lung-targeted delivery of a novel anti-cancer drug. Journal of Controlled Release, 388(Pt 1), 114299.
DOI Scopus1 WoS1
2025 Lee, J. H., Sumer Bayraktar, Z., Mittal, P., Donnellan, L., Young, C., Rushworth, R. L., . . . Meyer, E. J. (2025). Mass spectrometric detection of neutrophil elastase cleaved corticosteroid binding globulin and its association with Asn347 site glycosylation, in septic shock patients. Clinica Chimica Acta, 567(120108), 1-8.
DOI Scopus1 WoS1 Europe PMC1
2024 Seidel, J. D., Condina, M. R., Klingler Hoffmann, M., Young, C., Donnellan, L., Kyngdon, C., & Hoffmann, P. (2024). Development of an optimized LC-MS workflow for host cell protein characterization to support upstream process development. Journal of Proteome Research, 24(1), 234-243.
DOI Scopus1 WoS2
2024 Dilmetz, B. A., Desire, C. T., Meneses, J., Klingler-Hoffmann, M., Young, C., & Hoffmann, P. (2024). Impact of propagation time on yeast physiology during bottle conditioning of beer on an industrial scale. Food Chemistry, 435, 1-9.
DOI Scopus5 WoS4 Europe PMC2
2024 Sukjoi, W., Young, C., Acland, M., Siritutsoontorn, S., Roytrakul, S., Klingler Hoffmann, M., . . . Jitrapakdee, S. (2024). Proteomic analysis of holocarboxylase synthetase deficient-MDA-MB-231 breast cancer cells revealed the biochemical changes associated with cell death, impaired growth signaling, and metabolism. Frontiers in Molecular Biosciences, 10, 1-16.
DOI Scopus1 WoS1 Europe PMC1
2024 Li, C. M. Y., Briggs, M. T., Lee, Y. -R., Tin, T., Young, C., Pierides, J., . . . Fenix, K. (2024). Use of tryptic peptide MALDI mass spectrometry imaging to identify the spatial proteomic landscape of colorectal cancer liver metastases.. Clin Exp Med, 24(1), 53.
DOI Scopus9 WoS10 Europe PMC8
2024 Ngai, Y. T., Briggs, M. T., Mittal, P., Young, C., Parkinson-Lawrence, E., Klingler-Hoffmann, M., . . . Hoffmann, P. (2024). Mass spectrometry imaging protocol for spatial mapping of lipids, N-glycans and peptides in murine lung tissue. Rapid Communications in Mass Spectrometry, 38(9), e9721.
DOI Scopus6 WoS6 Europe PMC3
2024 Dilmetz, B. A., Brar, G., Desire, C. T., Meneses, J., Klingler Hoffmann, M., Young, C., & Hoffmann, P. (2024). Performance of different Saccharomyces strains on secondary fermentation during the production of beer. Foods, 13(16, article no. 2593), 1-17.
DOI Scopus2 WoS1
2023 Hua, Q. Q. H., Young, C., Pukala, T. L., Martino, J. C., Hoffmann, P., Gillanders, B. M., & Doubleday, Z. A. (2023). Better late than never: Optimising the proteomic analysis of field-collected octopus.. PloS one, 18(7), e0288084.
DOI Scopus1 WoS1 Europe PMC1
2023 Sebastian, S., Hoffmann, M. K., Howard, D., Young, C., Washington, J., Unterweger, H., . . . Kempson, I. (2023). Kinetic effects of transferrin-conjugated gold nanoparticles on the antioxidant glutathione-thioredoxin pathway. Antioxidants, 12(8, article no. 1617), 1-21.
DOI Scopus8 WoS8 Europe PMC7
2023 Dilmetz, B. A., Desire, C. T., Donnellan, L., Meneses, J., Klingler Hoffmann, M., & Young, C. (2023). Assessment of yeast physiology during industrial-scale brewing practices using the redox-sensitive dye resazurin. Yeast, 40(5-6), 171-181.
DOI Scopus3 WoS3 Europe PMC2
2023 Subramaniam, S., Joyce, P., Donnellan, L., Young, C., Wignall, A., Hoffmann, P., & Prestidge, C. A. (2023). Protein adsorption determines pulmonary cell uptake of lipid-based nanoparticles. Journal of Colloid and Interface Science, 641, 36-47.
DOI Scopus34 WoS34 Europe PMC25
2022 Donnellan, L., Young, C., Simpson, B. S., Acland, M., Dhillon, V. S., Costabile, M., . . . Deo, P. (2022). Proteomic analysis of methylglyoxal modifications reveals susceptibility of glycolytic enzymes to dicarbonyl stress. International Journal of Molecular Sciences, 23(7, article no. 3689), 1-15.
DOI Scopus27 WoS27 Europe PMC27
2022 Donnellan, L., Young, C., Simpson, B. S., Dhillon, V. S., Costabile, M., Hoffmann, P., . . . Deo, P. (2022). Methylglyoxal impairs sister chromatid separation in lymphocytes. International Journal of Molecular Sciences, 23(8, article no. 4139), 1-11.
DOI Scopus4 WoS4 Europe PMC3
2022 Acland, M., Lokman, N. A., Young, C., Anderson, D., Condina, M., Desire, C., . . . Klingler-Hoffmann, M. (2022). Chemoresistant Cancer Cell Lines Are Characterized by Migratory, Amino Acid Metabolism, Protein Catabolism and IFN1 Signalling Perturbations. Cancers, 14(11), 1-24.
DOI Scopus8 WoS8 Europe PMC9
2022 Lee, Y. -R., Briggs, M. T., Young, C., Condina, M. R., Kuliwaba, J. S., Anderson, P. H., & Hoffmann, P. (2022). Mass spectrometry imaging spatially identifies complex-type N-glycans as putative cartilage degradation markers in human knee osteoarthritis tissue. Analytical and Bioanalytical Chemistry, 414(26), 7597-7607.
DOI Scopus9 WoS9 Europe PMC7
2022 Pham, Q. N., Winter, M., Milanova, V., Young, C., Condina, M. R., Hoffmann, P., . . . Thierry, B. (2022). Magnetic enrichment of immuno-specific extracellular vesicles for mass spectrometry using biofilm-derived iron oxide nanowires. Nanoscale, 15(3), 1236-1247.
DOI Scopus8 WoS7 Europe PMC6
2021 Dilmetz, B. A., Lee, Y. R., Condina, M. R., Briggs, M., Young, C., Desire, C. T., . . . Hoffmann, P. (2021). Novel technical developments in mass spectrometry imaging in 2020: A mini review. Analytical Science Advances, 2(3-4), 225-237.
DOI Scopus28 WoS27 Europe PMC27
2021 Bastow, C. R., Bunting, M. D., Kara, E. E., McKenzie, D. R., Caon, A., Devi, S., . . . Comerford, I. (2021). Scavenging of soluble and immobilized CCL21 by ACKR4 regulates peripheral dendritic cell emigration. Proceedings of the National Academy of Sciences of USA, 118(17), e2025763118-1-e2025763118-9.
DOI Scopus32 WoS31 Europe PMC32
2021 Young, C., Condina, M. R., Briggs, M. T., Moh, E. S. X., Kaur, G., Oehler, M. K., & Hoffmann, P. (2021). In-House Packed Porous Graphitic Carbon Columns for Liquid Chromatography-Mass Spectrometry Analysis of <i>N-</i>Glycans.. Frontiers in chemistry, 9(article no. 653959), 653959.
DOI Scopus19 WoS17 Europe PMC14
2017 Hendriks, I. A., Lyon, D., Young, C., Jensen, L. J., Vertegaal, A. C. O., & Nielsen, M. L. (2017). Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation. Nature Structural and Molecular Biology, 24(3), 325-336.
DOI Scopus309 WoS297 Europe PMC311
2017 Young, C., Podtelejnikov, A. V., & Nielsen, M. L. (2017). Improved reversed phase chromatography of hydrophilic peptides from spatial and temporal changes in column temperature. Journal of Proteome Research, 16(6), 2307-2317.
DOI Scopus14 WoS14 Europe PMC11
2016 Martello, R., Leutert, M., Jungmichel, S., Bilan, V., Larsen, S. C., Young, C., . . . Nielsen, M. L. (2016). Proteome-wide identification of the endogenous ADP-ribosylome of mammalian cells and tissue. Nature Communications, 7(12917), 1-13.
DOI Scopus173 WoS123 Europe PMC179
2015 Madsen, C. T., Sylvestersen, K. B., Young, C., Larsen, S. C., Poulsen, J. W., Andersen, M. A., . . . Nielsen, M. L. (2015). Biotin starvation causes mitochondrial protein hyperacetylation and partial rescue by the SIRT3-like deacetylase Hst4p. Nature communications, 6(7726), 1-12.
DOI Scopus54 WoS52 Europe PMC46
2015 Zub, K. A., De Sousa, M. M. L., Sarno, A., Sharma, A., Demirovic, A., Rao, S., . . . Slupphaug, G. (2015). Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. PLoS One, 10(3), 1-20.
DOI Scopus68 WoS60 Europe PMC60
2014 Klimovskaia, I. M., Young, C., Strømme, C. B., Menard, P., Jasencakova, Z., Mejlvang, J., . . . Groth, A. (2014). Tousled-like kinases phosphorylate Asf1 to promote histone supply during DNA replication. Nature Communications, 5(1), 13 pages.
DOI Scopus62 WoS61 Europe PMC61
2013 Sylvestersen, K. B., Young, C., & Nielsen, M. L. (2013). Advances in characterizing ubiquitylation sites by mass spectrometry. Current Opinion in Chemical Biology, 17(1), 49-58.
DOI Scopus51 WoS48 Europe PMC43
2013 Vendel Nielsen, L., Krogager, T. P., Young, C., Ferreri, C., Chatgilialoglu, C., Nørregaard Jensen, O., & Enghild, J. J. (2013). Effects of Elaidic Acid on Lipid Metabolism in HepG2 Cells, Investigated by an Integrated Approach of Lipidomics, Transcriptomics and Proteomics. Plos One, 8(9), 17 pages.
DOI Scopus44 WoS39 Europe PMC30
2013 Poulsen, J. W., Madsen, C. T., Young, C., Poulsen, F. M., & Nielsen, M. L. (2013). Using guanidine-hydrochloride for fast and efficient protein digestion and single-step affinity-purification mass spectrometry. Journal of Proteome Research, 12(2), 1020-1030.
DOI Scopus42 WoS40 Europe PMC41
2012 Young, C., & Truman, P. (2012). Proteins isolated with TRIzol are compatible with two-dimensional electrophoresis and mass spectrometry analyses. Analytical Biochemistry, 421(1), 330-332.
DOI Scopus20 WoS18 Europe PMC16
2012 Wagner, S. A., Beli, P., Weinert, B. T., Schölz, C., Kelstrup, C. D., Young, C., . . . Choudhary, C. (2012). Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Molecular and Cellular Proteomics, 11(12), 1578-1585.
DOI Scopus257 WoS252 Europe PMC233
2012 Kelstrup, C. D., Young, C., Lavallee, R., Nielsen, M. L., & Olsen, J. V. (2012). Optimized fast and sensitive acquisition methods for shotgun proteomics on a quadrupole orbitrap mass spectrometer. Journal of Proteome Research, 11(6), 3487-3497.
DOI Scopus246 WoS233 Europe PMC242
2012 Poulsen, J. W., Madsen, C. T., Young, C., Kelstrup, C. D., Grell, H. C., Henriksen, P., . . . Nielsen, M. L. (2012). Comprehensive profiling of proteome changes upon sequential deletion of deubiquitylating enzymes. Journal of Proteomics, 75(13), 3886-3897.
DOI Scopus19 WoS16 Europe PMC15
2012 Krogager, T. P., Nielsen, L. V., Bak, S., Young, C., Ferreri, C., Jensen, O. N., . . . Enghild, J. J. (2012). Identification of a potential biomarker panel for the intake of the common dietary trans fat elaidic acid (trans{increment}9-C18:1). Journal of Proteomics, 75(9), 2685-2696.
DOI Scopus6 WoS6 Europe PMC6
2011 Ungureanu, D., Wu, J., Pekkala, T., Niranjan, Y., Young, C., Jensen, O. N., . . . Silvennoinen, O. (2011). The pseudokinase domain of JAK2 is a dual-specificity protein kinase that negatively regulates cytokine signaling. Nature Structural and Molecular Biology, 18(9), 971-976.
DOI Scopus225 WoS215 Europe PMC201
2010 Ye, J., Zhang, X., Young, C., Zhao, X., Hao, Q., Cheng, L., & Jensen, O. N. (2010). Optimized IMAC-IMAC protocol for phosphopeptide recovery from complex biological samples. Journal of Proteome Research, 9(7), 3561-3573.
DOI Scopus105 WoS103 Europe PMC79
2009 Young, C., Truman, P., Boucher, M., Keyzers, R. A., Northcote, P., & Jordan, T. W. (2009). The algal metabolite yessotoxin affects heterogeneous nuclear ribonucleoproteins in HepG2 cells. Proteomics, 9(9), 2529-2542.
DOI Scopus21 WoS20 Europe PMC14

Year Citation
2017 Larsen, S. C., Leutert, M., Bilan, V., Martello, R., Jungmichel, S., Young, C., . . . Nielsen, M. L. (2017). Proteome-wide identification of in vivo ADP-ribose acceptor sites by liquid chromatography–tandem mass spectrometry. In Methods in Molecular Biology (Vol. 1608, pp. 149-162). Springer New York.
DOI Scopus28 Europe PMC29

Year Citation
2025 Scheer, K., Yeo, E., Shard, C., Lenin, S., Ormsby, R., Skarne, N., . . . Gomez, G. (2025). Growth rates of patient-derived tumor organoids reflect glioblastoma patients' heterogeneity in survival. In NEURO-ONCOLOGY Vol. 27 (pp. v479). OXFORD UNIV PRESS INC.
DOI

Year Citation
2026 Scanlan, J., Mittal, P., Washington, J., Young, C., Oehler, M. K., Hoffmann, P., & Klingler-Hoffmann, M. (2026). Ascites-Driven Modulation of Cell Phenotypes and Proteomes: Implications for Cancer Progression.
DOI
2022 Pham, Q. N., Winter, M., Milanova, V., Young, C., Condina, M., Hoffmann, P., . . . Thierry, B. (2022). Magnetic Enrichment of Immuno-Specific Extracellular Vesicles for Mass Spectrometry Using Biofilm-Derived Iron Oxide Nanowires.
DOI

Date Role Research Topic Program Degree Type Student Load Student Name
2023 Co-Supervisor 111153 - Mass Spectrometry assays for protein biomarker for the early detection of ovarian cancer - Master Full Time Mr Nicholas Chappell
2022 Co-Supervisor Mass spectrometry and spectroscopic analytical approaches for directed monoclonal antibody formulation development - Doctorate Full Time Abdulafeez Akinloye
2022 Co-Supervisor Process analytical technologies for the development and monitoring of cell culture processes in biopharmaceutical production - Doctorate Full Time Mr Janik Seidel
2021 Co-Supervisor Functional Analysis and Quantification of Biopharmaceuticals in Tissues by Mass Spectrometry Imaging - Doctorate Part Time Ms Sammi Ngai

Connect With Me

External Profiles

Other Links