John Bruning

APrf John Bruning

Associate Professor

School of Biological Sciences

College of Science

Eligible to supervise Masters and PhD - email supervisor to discuss availability.

I am an expert in structural biology of proteins and protein interactions who has solved the crystal structures of dozens of proteins and protein-ligand complexes. My research has been published in high impact journals such as Nature, Nature Chemical Biology, Nature Communications, Nature Structural and Molecular Biology, and Angewandte Chemie, and has published ~120 manuscripts. The Laboratory of Protein Crystallography at UoA is one of the few in the world that can purify human PCNA in the quantities and quality necessary for structural studies.

I head the Laboratory of Protein Crystallography which studeis Nuclear Receptors, Microbial Enzymology, and Broad-Based Cancer Therapeutics. Understanding human disease requires knowledge of the structure-function relationships in protein sensor modulation.This knowledge is incomplete on a broad spectrum of biological systems including nuclear receptor (NR) signalling, proteins involved in cancer biology, and pathogen protein machinery. Our research is focused on understanding these classes of protein interactions on an atomic level. In addition to gaining a basic understanding of protein mechanism, we also focus on structure guided drug design. In our work in understanding NR signalling, we have uncovered new paradigms in NR allostery. These allosteric modulations can now be further studied on a broader scale and harnessed to employ structure guided drug design. Work in the tuberculosis (TB) structural biology consortium has led to a quantum leap in understanding enzymology of the pathogen allowing for new chemotherapy development. We wish to use similar methodology to study a fungal pathogen for the discovery of new anti-fungal molecules.Our research with proteins of the human replication fork, such as PCNA (Proliferating Cellular Nuclear Antigen) has uncovered atomic determinants of these protein modulators that can be used as new paradigms for cancer therapeutic targeting. We will continue this research to aid in the discovery of alternative cancer therapeutic targets and seek to understand their structure-function relationship.

Date Position Institution name
2024 - ongoing Associate Professor The University of Adelaide
2021 - ongoing Editor Journal PPAR research
2016 - ongoing Adjunct Professor The Scripps Research Institute
2016 - ongoing Senior Lecturer Level C (Tenured) The University of Adelaide
2016 - ongoing Head of the Laboratory of Protein Crystallography The University of Adelaide
2012 - 2015 Lecturer Level B The University of Adelaide
2007 - 2012 Associate Research Scientist Texas A&M University
2005 - 2007 Post-doctoral Research Associate Scripps Research Institute

Date Institution name Country Title
1998 - 2004 Rice University United States PhD
1994 - 1997 Texas A&M University USA BSc

Date Title Institution Country
2007 - 2012 Assistant Research Scientist Texas A&M USA
2005 - 2007 Post-doctoral Research Assistant The Scripps Research Institute USA

Year Citation
2025 Kirk, A. M., Stok, J. E., Wong, S. H., Coleman, T., Challinor, V. L., Herring, J. N., . . . De Voss, J. J. (2025). An Asynchronous, Concerted Mechanism for Cytochrome P450-Catalyzed Dehydrogenation: A Combined Deuterium Labeling and QM/MM Study. ACS Catalysis, 15(2), 1274-1286.
DOI Scopus4 WoS4
2025 Podgorski, M. N., Martínez-Castro, L., Bruning, J. B., Campbell, E. C., Maréchal, J. -D., & Bell, S. G. (2025). Investigating the Correlation between Product Release and Solvation in Cytochrome P450 Enzymes. ACS Catalysis, 15(4), 2867-2884.
DOI Scopus2
2025 Holroyd, D. L., Kumar, A., Vasquez, E., Masic, V., von Itzstein, M., Bruning, J. B., & Hansman, G. S. (2025). Antigenic structural analysis of bat and human norovirus protruding (P) domains. Journal of Virology, 99(4), e0197124-1-e0197124-6.
DOI Scopus1 WoS1 Europe PMC1
2025 Pederick, J. L., Kumar, A., Pukala, T. L., & Bruning, J. B. (2025). Functional and structural characterization of Staphylococcus aureus N-acetylglucosamine 1-phosphate uridyltransferase (GlmU) reveals a redox-sensitive acetyltransferase activity.. Protein Sci, 34(4), e70111.
DOI Scopus1 WoS1 Europe PMC3
2025 Lagonik, E., Page, E. C., Bruning, J. B., Eadie, L. N., Heatley, S. L., Greenwood, M., . . . White, D. L. (2025). Activity of STAMP inhibitors in ABL2 rearranged Acute Lymphoblastic Leukemia is dependent on the Abl2 SH3 domain. Blood Neoplasia, 2(3), 100109.
DOI Scopus1 Europe PMC1
2025 Doherty, D. Z., De Voss, J. J., Bruning, J. B., & Bell, S. G. (2025). Evolutionary insights into the selectivity of sterol oxidising cytochrome P450 enzymes based on ancestral sequence reconstruction. Chemical Science, 16(24), 11110-11122.
DOI Scopus1 WoS1 Europe PMC1
2025 Pederick, J. L., & Bruning, J. B. (2025). Structural comparison of androstenediol recognition by the human estrogen receptor ligand binding domains. Molecular and Cellular Endocrinology, 607, 112588.
DOI Scopus1 WoS1
2025 Haywood, J., Breese, K. J., McDougal, D. P., Verdonk, C., Partridge, A., Lo, A. F., . . . Mylne, J. S. (2025). Structural insights into a plant-conserved DHFR-TS reveal a selective herbicide target. Molecular Plant, 18(8), 1294-1309.
DOI
2025 Holroyd, D. L., Bruning, J. B., von Itzstein, M., & Hansman, G. S. (2025). Structural constraints prevent a bat norovirus from binding to histo-blood group antigen co-factors. Journal of Virology, 99(9), e00995-25-1-e00995-25-5.
DOI
2025 Xie, J., Shen, K., Liang, W., Kuang, Z., Shrestha, R. K., Hanson, A. R., . . . Selth, L. A. (2025). Protein disulfide isomerases regulate androgen receptor stability and promote prostate cancer cell growth and survival. Proceedings of the National Academy of Sciences of the United States of America, 122(42), e2509222122-1-e2509222122-12.
DOI
2025 McDougal, D. P., Pederick, J. L., Novick, S. J., Jovcevski, B., Warrender, A. K., Pascal, B. D., . . . Bruning, J. B. (2025). A ternary switch model governing ERα ligand binding domain conformation. Nature Communications, 16(1), 13 pages.
DOI
2024 Lee, J. H. Z., Bruning, J. B., & Bell, S. G. (2024). An In Crystallo Reaction with an Engineered Cytochrome P450 Peroxygenase**. Chemistry: A European Journal, 30(8), e202303335-1-e202303335-8.
DOI Scopus3 WoS3
2024 Clayton-Cuch, D., Yu, L., McDougal, D., Burbidge, C. A., Bruning, J. B., Bradley, D., . . . Bulone, V. (2024). Biochemical and in silico characterization of glycosyltransferases from red sweet cherry (Prunus avium L.) reveals their broad specificity toward phenolic substrates. Food Chemistry: Molecular Sciences, 8, 100193.
DOI Scopus5 WoS5 Europe PMC1
2024 Mohamed, H., Child, S. A., Doherty, D. Z., Bruning, J. B., & Bell, S. G. (2024). Structural determination and characterisation of the CYP105Q4 cytochrome P450 enzyme from Mycobacterium marinum. Arch Biochem Biophys, 754, 109950-1-109950-11.
DOI Scopus3 WoS3 Europe PMC1
2024 Pederick, J. L., Frkic, R. L., McDougal, D. P., & Bruning, J. B. (2024). A structural basis for the activation of peroxisome proliferator-activated receptor gamma (PPARγ) by perfluorooctanoic acid (PFOA). Chemosphere, 354, 141723-1-141723-12.
DOI Scopus10 Europe PMC12
2024 Wallach, I., Bernard, D., Nguyen, K., Ho, G., Tumes, D., Cildir, G., & Heifets, A. (2024). AI is a viable alternative to high throughput screening: a 318-target study. Scientific Reports, 14(1), 1-16.
DOI Scopus64 WoS70 Europe PMC68
2024 Podgorski, M. N., Akter, J., Churchman, L. R., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2024). Engineering Peroxygenase Activity into Cytochrome P450 Monooxygenases through Modification of the Oxygen Binding Region. ACS Catalysis, 14(10), 7426-7443.
DOI Scopus15 WoS15
2024 Lee, J. H. Z., Coleman, T., Mclean, M. A., Podgorski, M. N., Hayball, E. F., Stone, I. S. J., . . . Bell, S. G. (2024). Selective α-Hydroxyketone Formation and Subsequent C–C Bond Cleavage by Cytochrome P450 Monooxygenase Enzymes. ACS Catalysis, 14(11), 8958-8971.
DOI Scopus6 WoS6 Europe PMC2
2024 Giles, E., Heifets, A., Artía, Z., Inde, Z., Liu, Z., Zhang, Z., . . . Waggoner, S. N. (2024). Correction to: AI is a viable alternative to high throughput screening: a 318-target study (Scientific Reports, (2024), 14, 1, (7526), 10.1038/s41598-024-54655-z). Scientific Reports, 14(1), 21579.
DOI
2024 Pederick, J. L., Vandborg, B. C., George, A., Bovermann, H., Boyd, J. M., Freundlich, J. S., & Bruning, J. B. (2024). Identification of cysteine metabolism regulator (CymR)-derived pentapeptides as nanomolar inhibitors of Staphylococcus aureus O-acetyl-ʟ-serine sulfhydrylase (CysK).. bioRxiv.
DOI
2024 Pederick, J. L., Vandborg, B. C., George, A., Bovermann, H., Boyd, J. M., Freundlich, J. S., & Bruning, J. B. (2024). Identification of Cysteine Metabolism Regulator (CymR)-Derived Pentapeptides as Nanomolar Inhibitors of Staphylococcus aureus O-Acetyl-l-serine Sulfhydrylase (CysK).. ACS Infect Dis, 11(1), 238-248.
DOI
2023 Vandborg, B., Holroyd, D. L., Pukala, T., & Bruning, J. B. (2023). Production of recombinant human proliferating cellular nuclear antigen (PCNA) for structural and biophysical characterization. Protein Expression and Purification, 212, 1-7.
DOI Scopus2 WoS2 Europe PMC2
2023 Salaemae, W., Thompson, A. P., Gaiser, B. I., Lee, K. J., Huxley, M. T., Sumby, C. J., . . . Wegener, K. L. (2023). Fortuitous <i>In Vitro</i> Compound Degradation Produces a Tractable Hit against <i>Mycobacterium tuberculosis</i> Dethiobiotin Synthetase: A Cautionary Tale of What Goes In Does Not Always Come Out. ACS Chemical Biology, 18(9), 1985-1992.
DOI
2023 Podgorski, M. N., Lee, J. H. Z., Harbort, J. S., Nguyen, G. T. H., Doherty, D. Z., Donald, W. A., . . . Bell, S. G. (2023). Characterisation of the heme aqua-ligand coordination environment in an engineered peroxygenase cytochrome P450 variant. Journal of Inorganic Biochemistry, 249, 1-13.
DOI Scopus4 WoS4 Europe PMC1
2023 Becker, R., Pederick, J. L., Dawes, E. G., Bruning, J. B., & Abell, A. D. (2023). Structure-Guided Design and Synthesis of ATP-competitive NAcyl-substituted Sulfamide D-Alanine-D-Alanine Ligase Inhibitors. Bioorganic and Medicinal Chemistry, 96, 14 pages.
DOI Scopus2 WoS2
2023 Vandborg, B. C., Horsfall, A. J., Pederick, J. L., Abell, A. D., & Bruning, J. B. (2023). Towards a High-Affinity Peptidomimetic Targeting Proliferating Cell Nuclear Antigen from Aspergillus fumigatus. Journal of Fungi, 9(11), 16 pages.
DOI
2023 Henderson, S. W., Nakayama, Y., Whitelaw, M. L., Bruning, J. B., Anderson, P. A., Tyerman, S. D., . . . Yool, A. J. (2023). Proteoliposomes reconstituted with human aquaporin-1 reveal novel single ion channel properties. Biophysical Reports, 3(1), 100100-1-100100-15.
DOI Scopus4 WoS4 Europe PMC3
2023 Pederick, J. L., Klose, J., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2023). <i>Escherichia coli</i> YgiC and YjfC Possess Peptide─Spermidine Ligase Activity. Biochemistry, 62(4), 899-911.
DOI Scopus5 WoS5 Europe PMC5
2023 Stachura, D. L., Nguyen, S., Polyak, S. W., Jovcevski, B., Bruning, J. B., & Abell, A. D. (2023). Structural Study of Potent Triazole-Based Inhibitors of <i>Staphylococcus aureus</i> Biotin Protein Ligase. ACS Medicinal Chemistry Letters, 14(3), 285-290.
DOI Scopus2 WoS1 Europe PMC1
2023 Ghith, A., Bruning, J. B., & Bell, S. G. (2023). The catalytic activity and structure of the lipid metabolizing CYP124 cytochrome P450 enzyme from Mycobacterium marinum.. Archives of biochemistry and biophysics, 737, 109554.
DOI Scopus5 WoS4 Europe PMC2
2023 Salaemae, W., Thompson, A., Gaiser, B., Lee, K. J., Huxley, M., Sumby, C., . . . Wegener, K. (2023). Fortuitous<i>in vitro</i>compound degradation produces a tractable hit against<i>Mycobacterium tuberculosis</i>dethiobiotin synthetase: a cautionary tale of what goes in, does not always come out.
DOI
2023 Miller, J. C., Lee, J. H. Z., Mclean, M. A., Chao, R. R., Stone, I. S. J., Pukala, T. L., . . . Bell, S. G. (2023). Engineering C–C Bond Cleavage Activity into a P450 Monooxygenase Enzyme. Journal of the American Chemical Society, 145(16), 9207-9222.
DOI Scopus20 WoS20 Europe PMC9
2023 Coleman, T., Podgorski, M. N., Doyle, M. L., Scaffidi-Muta, J. M., Campbell, E. C., Bruning, J. B., . . . Bell, S. G. (2023). Cytochrome P450-catalyzed oxidation of halogen-containing substrates.. Journal of Inorganic Biochemistry, 244, 12 pages.
DOI Scopus9 WoS10 Europe PMC5
2023 Frkic, R. L., Pederick, J. L., Horsfall, A. J., Jovcevski, B., Crame, E. E., Kowalczyk, W., . . . Bruning, J. B. (2023). PPARγ Corepression Involves Alternate Ligand Conformation and Inflation of H12 Ensembles.. ACS Chemical Biology, 18(5), 1115-1123.
DOI Scopus6 WoS6 Europe PMC10
2023 Ghith, A., Bruning, J. B., & Bell, S. G. (2023). The oxidation of cholesterol derivatives by the CYP124 and CYP142 enzymes from Mycobacterium marinum.. J Steroid Biochem Mol Biol, 231, 106317-1-106317-12.
DOI Scopus3 WoS4 Europe PMC2
2023 Pantong, W., Pederick, J. L., Maenpuen, S., Tinikul, R., Jayapalan, J. J., Jovcevski, B., . . . Salaemae, W. (2023). Biochemical and Structural Characterization of Meningococcal Methylenetetrahydrofolate Reductase. Protein Science, 32(6), e4654-1-e4654-13.
DOI Scopus1 WoS1 Europe PMC2
2023 Clayton-Cuch, D., McDougal, D., Schwerdt, J. G., Yu, L., Shirley, N., Bradley, D., . . . Bulone, V. (2023). Identification and characterisation of MdUGT78T2 as a galactosyltransferase with dual activity on flavonol and anthocyanidin substrates in red-skinned apple fruit (Malus domestica L.). Food Chemistry, 424, 12 pages.
DOI Scopus6 WoS4 Europe PMC4
2023 Podgorski, M. N., Keto, A. B., Coleman, T., Bruning, J. B., De Voss, J. J., Krenske, E. H., & Bell, S. G. (2023). The Oxidation of Oxygen and Sulfur-Containing Heterocycles by Cytochrome P450 Enzymes. Chemistry: A European Journal, 29(50), e202301371-1-e202301371-15.
DOI Scopus7 WoS8 Europe PMC1
2023 Horsfall, A. J., Chav, T., Pederick, J. L., Kikhtyak, Z., Vandborg, B. C., Kowalczyk, W., . . . Bruning, J. B. (2023). Designing Fluorescent Nuclear Permeable Peptidomimetics to Target Proliferating Cell Nuclear Antigen. Journal of Medicinal Chemistry, 66(15), 10354-10363.
DOI Scopus1 WoS1 Europe PMC1
2023 McDougal, D. P., Rajapaksha, H., Pederick, J. L., & Bruning, J. B. (2023). warpDOCK: Large-Scale Virtual Drug Discovery Using Cloud Infrastructure. ACS Omega, 8(32), 28932-29846.
DOI Scopus3 WoS2 Europe PMC2
2023 Pederick, J. L., Woolman, J. C., & Bruning, J. B. (2023). Comparative functional and structural analysis of Pseudomonas aeruginosa ᴅ-alanine-ᴅ-alanine ligase isoforms as prospective antibiotic targets.. The FEBS journal, 290(23), 5536-5553.
DOI Scopus1 WoS1 Europe PMC1
2022 Coleman, T., Kirk, A. M., Lee, J. H. Z., Doherty, D. Z., Bruning, J. B., Krenske, E. H., . . . Bell, S. G. (2022). Different geometric requirements for cytochrome P450-catalyzed aliphatic versus aromatic hydroxylation results in chemoselective oxidation. ACS Catalysis, 12(2), 1258-1267.
DOI Scopus26 WoS25
2022 Podgorski, M. N., Harbort, J. S., Lee, J. H. Z., Nguyen, G. T. H., Bruning, J. B., Donald, W. A., . . . Bell, S. G. (2022). An altered heme environment in an engineered cytochrome P450 enzyme enables the switch from monooxygenase to peroxygenase activity. ACS Catalysis, 12(3), 1614-1625.
DOI Scopus47 WoS44
2022 Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2022). Structural insights into the antifungal drug target guanosine monophosphate synthase from Aspergillus fumigatus. Acta Crystallographica Section D Structural Biology, 78(2), 1-12.
DOI Scopus8 WoS7 Europe PMC7
2022 Bersten, D. C., Sullivan, A. E., McDougal, D., Breen, J., Fitzsimmons, R. L., Muscat, G. E. O., . . . Whitelaw, M. L. (2022). Core and Flanking bHLH-PAS:DNA interactions mediate specificity and drive obesity.
DOI Europe PMC1
2022 Nguyen, S., Jovcevski, B., Truong, J. Q., Pukala, T. L., & Bruning, J. B. (2022). A structural model of the human plasminogen and Aspergillus fumigatus enolase complex.. Proteins, 90(8), 1509-1520.
DOI Scopus1 WoS2 Europe PMC1
2022 Mohamed, H., Child, S. A., Bruning, J. B., & Bell, S. G. (2022). A comparison of the bacterial CYP51 cytochrome P450 enzymes from Mycobacterium marinum and Mycobacterium tuberculosis. The Journal of Steroid Biochemistry and Molecular Biology, 221, 106097-1-106097-14.
DOI Scopus5 WoS5 Europe PMC2
2022 Button, E. L., Rossi, J. J., McDougal, D. P., Bruning, J. B., Peet, D., Bersten, D., . . . Whitelaw, M. (2022). Characterization of functionally deficient SIM2 variants found in patients with neurological phenotypes.. The Biochemical journal, 479(13), 1441-1454.
DOI Scopus5 WoS3 Europe PMC3
2022 Downes, C. E., McClure, B. J., McDougal, D. P., Heatley, S. L., Bruning, J. B., Thomas, D., . . . White, D. L. (2022). JAK2 Alterations in Acute Lymphoblastic Leukemia: Molecular Insights for Superior Precision Medicine Strategies. Frontiers in Cell and Developmental Biology, 10(942053), 1-34.
DOI Scopus27 WoS24 Europe PMC22
2022 Ghith, A., Doherty, D. Z., Bruning, J. B., Russell, R. A., De Voss, J. J., & Bell, S. G. (2022). The Structures of the Steroid Binding CYP142 Cytochrome P450 Enzymes from Mycobacterium ulcerans and Mycobacterium marinum. ACS Infectious Diseases, 8(8), 1606-1617.
DOI Scopus8 WoS8 Europe PMC3
2022 Pederick, J. L., Horsfall, A. J., Jovcevski, B., Klose, J., Abell, A. D., Pukala, T. L., & Bruning, J. B. (2022). Discovery of an ʟ-amino acid ligase implicated in Staphylococcal sulfur amino acid metabolism. Journal of Biological Chemistry, 298(10), 102392-1-102392-16.
DOI Scopus6 WoS7 Europe PMC5
2022 Coleman, T., Lee, J., Kirk, A. M., Doherty, D. Z., Podgorski, M. N., Pinidiya, D. K., . . . Bell, S. G. (2022). Enabling Aromatic Hydroxylation in a Cytochrome P450 Monooxygenase Enzyme through Protein Engineering. Chemistry: A European Journal, 28(67), 1-13.
DOI Scopus19 WoS17 Europe PMC9
2022 Podgorski, M. N., Coleman, T., Churchman, L. R., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2022). Investigating the active oxidants involved in cytochrome P450 catalyzed sulfoxidation reactions. Chemistry: A European Journal, 28(72), 1-14.
DOI Scopus17 WoS17 Europe PMC9
2022 Coleman, T., Doherty, D. Z., Zhang, T., Podgorski, M. N., Qiao, R., Lee, J. H. Z., . . . Bell, S. G. (2022). Exploring the Factors which Result in Cytochrome P450 Catalyzed Desaturation Versus Hydroxylation. Chemistry: An Asian Journal, 17(24), 1-15.
DOI Scopus13 WoS13 Europe PMC6
2022 Stachura, D. L., Nguyen, S., Polyak, S. W., Jovcevski, B., Bruning, J. B., & Abell, A. D. (2022). A New 1,2,3-triazole scaffold with improved potency against Staphylococcus aureus biotin protein ligase. ACS Infectious Diseases, 8(12), 2579-2585.
DOI Scopus5 WoS4 Europe PMC4
2021 Pederick, J. L., & Bruning, J. B. (2021). An antimony-phosphomolybdate microassay of ATPase activity through the detection of inorganic phosphate. Analytical Biochemistry, 623, 114170.
DOI Scopus10 WoS11 Europe PMC6
2021 Horsfall, A. J., Chav, T., Bruning, J. B., & Abell, A. D. (2021). A turn-on fluorescent PCNA sensor. Bioorganic & Medicinal Chemistry Letters, 41, 128031-1-128031-6.
DOI Scopus2 WoS2 Europe PMC1
2021 Xie, J., De Poi, S. P., Humphrey, S. J., Hein, L. K., Bruning, J. B., Pan, W., . . . Proud, C. G. (2021). TSC-insensitive Rheb mutations induce oncogenic transformation through a combination of constitutively active mTORC1 signalling and proteome remodelling. Cellular and Molecular Life Sciences, 78(8), 4035-4052.
DOI Scopus6 WoS6 Europe PMC5
2021 Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Simplified heavy-atom derivatization of protein structures via co-crystallization with the MAD tetragon tetrabromoterephthalic acid. Acta Crystallographica Section F Structural Biology Communications, 77(5), 156-162.
DOI Scopus2 WoS2 Europe PMC2
2021 Tavakoli Shirazi, P., Eadie, L. N., Page, E. C., Heatley, S. L., Bruning, J. B., & White, D. L. (2021). Constitutive JAK/STAT signaling is the primary mechanism of resistance to JAKi in TYK2-rearranged acute lymphoblastic leukemia. Cancer Letters, 512, 28-37.
DOI Scopus11 WoS12 Europe PMC12
2021 Horsfall, A. J., Vandborg, B. A., Kowalczyk, W., Chav, T., Scanlon, D. B., Abell, A. D., & Bruning, J. B. (2021). Unlocking the PIP-box: A peptide library reveals interactions that drive high affinity binding to human PCNA.. The Journal of biological chemistry, 296, 1-16.
DOI Scopus15 WoS14 Europe PMC16
2021 Horsfall, A. J., McDougal, D. P., Scanlon, D. B., Bruning, J. B., & Abell, A. D. (2021). Approaches to introduce helical structure in cysteine-containing peptides with a bimane group. Chembiochem, 22(17), 2711-2720.
DOI Scopus4 WoS3 Europe PMC2
2021 Qu, Y., Zhang, B., Wang, Y., Yin, S., Pederick, J. L., Bruning, J. B., . . . Bi, J. (2021). Immunogenicity study of engineered ferritins with C- and N-terminus insertion of Epstein-Barr nuclear antigen 1 epitope. Vaccine, 39(34), 4830-4841.
DOI Scopus8 WoS6 Europe PMC6
2021 Downes, C. E. J., McClure, B. J., Bruning, J. B., Page, E., Breen, J., Rehn, J., . . . White, D. L. (2021). Acquired JAK2 mutations confer resistance to JAK inhibitors in cell models of acute lymphoblastic leukemia. NPJ Precision Oncology, 5(1), 1-13.
DOI Scopus21 WoS20 Europe PMC16
2021 Frkic, R. L., Richter, K., & Bruning, J. B. (2021). The therapeutic potential of inhibiting PPARγ phosphorylation to treat type 2 diabetes.. Journal of Biological Chemistry, 297(3), 101030-1-101030-9.
DOI Scopus56 WoS52 Europe PMC37
2021 Marshall, A. C., & Bruning, J. B. (2021). Engineering potassium activation into biosynthetic thiolase. The Biochemical journal, 478(15), 3047-3062.
DOI Scopus2 WoS2 Europe PMC1
2021 Chao, R., Lau, I., Coleman, T., Churchman, L. R., Child, S. A., Lee, J. H. Z., . . . Bell, S. G. (2021). The stereoselective oxidation of para-substituted benzenes by a cytochrome P450 biocatalyst. Chemistry: A European Journal, 27(59), 14765-14777.
DOI Scopus9 WoS9 Europe PMC5
2021 Horsfall, A. J., Vandborg, B., Kikhtyak, Z., Scanlon, D. B., Tilley, W. D., Hickey, T. E., . . . Abell, A. D. (2021). A cell permeable bimane-constrained PCNA-interacting peptide. RSC Chemical Biology, 2(5), 1499-1508.
DOI Scopus5 WoS5 Europe PMC3
2021 Schumann, N. C., Lee, K. J., Thompson, A. P., Salaemae, W., Pederick, J. L., Avery, T., . . . Abell, A. D. (2021). Inhibition of Mycobacterium tuberculosis dethiobiotin synthase (MtDTBS): toward next-generation antituberculosis agents. ACS Chemical Biology, 16(11), 2339-2347.
DOI Scopus7 WoS7 Europe PMC7
2021 Podgorski, M. N., Coleman, T., Giang, P. D., Wang, C. R., Bruning, J. B., Bernhardt, P. V., . . . Bell, S. G. (2021). To be, or not to be, an Inhibitor: a comparison of azole interactions with and oxidation by a cytochrome P450 enzyme. Inorganic Chemistry, 61(1), 236-245.
DOI Scopus16 WoS16 Europe PMC10
2021 Slynko, I., Nguyen, S., Hamilton, E. M. C., Wisse, L. E., de Esch, I. J. P., de Graaf, C., . . . van der Knaap, M. S. (2021). Vanishing white matter: Eukaryotic initiation factor 2B model and the impact of missense mutations.. Molecular genetics & genomic medicine, 9(3), 1-15.
DOI Scopus26 WoS25 Europe PMC23
2021 Nguyen, S., Jovcevski, B., Pukala, T. L., & Bruning, J. B. (2021). Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase.. The FEBS journal, 288(7), 2398-2417.
DOI Scopus8 WoS9 Europe PMC7
2021 Nguyen, S., Truong, J. Q., & Bruning, J. B. (2021). Targeting Unconventional Pathways in Pursuit of Novel Antifungals.. Front Mol Biosci, 7, 1-16.
DOI Scopus18 WoS14 Europe PMC10
2021 Truong, J. Q., Nguyen, S., Bruning, J. B., & Shearwin, K. E. (2021). Derivatization of protein crystals with I3C using random microseed matrix screening. Journal of Visualized Experiments, 2021(167), 1-19.
DOI WoS1 Europe PMC1
2021 Coleman, T., Kirk, A. M., Chao, R. R., Podgorski, M. N., Harbort, J. S., Churchman, L. R., . . . Bell, S. G. (2021). Understanding the Mechanistic Requirements for Efficient and Stereoselective Alkene Epoxidation by a Cytochrome P450 Enzyme. ACS Catalysis, 11(4), 1995-2010.
DOI Scopus49 WoS44
2020 Jia, Y., Shen, G., Nguyen, S., Zhang, Y., Huang, K. -S., Ho, H. -Y., . . . Wang, W. -L. (2020). Analysis of the mutation dynamics of SARS-CoV-2 reveals the spread history and emergence of RBD mutant with lower ACE2 binding affinity.
DOI Europe PMC9
2020 Horsfall, A. J., Abell, A. D., & Bruning, J. (2020). Targeting PCNA with peptide mimetics for therapeutic purposes. ChemBioChem, 21(4), 442-450.
DOI Scopus35 WoS30 Europe PMC30
2020 Podgorski, M. N., Coleman, T., Chao, R. R., De Voss, J. J., Bruning, J. B., & Bell, S. G. (2020). Investigation of the requirements for efficient and selective cytochrome P450 monooxygenase catalysis across different reactions. Journal of Inorganic Biochemistry, 203, 110913-1-110913-13.
DOI Scopus30 WoS30 Europe PMC19
2020 Podgorski, M. N., Harbort, J. S., Coleman, T., Stok, J. E., Yorke, J. A., Wong, L. -L., . . . Bell, S. G. (2020). Biophysical techniques for distinguishing ligand binding modes in cytochrome P450 monooxygenases. Biochemistry, 59(9), 1038-1050.
DOI Scopus26 WoS25 Europe PMC16
2020 Coleman, T., Stok, J. E., Podgorski, M. N., Bruning, J. B., De Voss, J. J., & Bell, S. G. (2020). Structural insights into the role of the acid-alcohol pair of residues required for dioxygen activation in cytochrome P450 enzymes. JBIC Journal of Biological Inorganic Chemistry, 25(4), 583-596.
DOI Scopus36 WoS40 Europe PMC22
2020 Pederick, J. L., Thompson, A. P., Bell, S. G., & Bruning, J. B. (2020). D-alanine-D-alanine ligase as a model for the activation of ATP-grasp enzymes by monovalent cations.. Journal of Biological Chemistry, 295(23), 7894-7904.
DOI Scopus32 WoS27 Europe PMC27
2020 Child, S. A., Ghith, A., Bruning, J. B., & Bell, S. G. (2020). A comparison of steroid and lipid binding cytochrome P450s from Mycobacterium marinum and Mycobacterium tuberculosis. Journal of Inorganic Biochemistry, 209, 1-12.
DOI Scopus15 WoS14 Europe PMC7
2020 Xie, J., De Poi, S., Humphrey, S., Hein, L., Bruning, J., Pan, W., . . . Proud, C. (2020). TSC-Insensitive Rheb Mutations Induce Oncogenic Transformation Through a Combination of Hyperactive mTORC1 Signalling and Metabolic Reprogramming.
DOI
2019 Truong, J. Q., Panjikar, S., Shearwin-Whyatt, L., Bruning, J. B., & Shearwin, K. E. (2019). Combining random microseed matrix screening and the magic triangle for the efficient structure solution of a potential lysin from bacteriophage P68. Acta crystallographica Section D. Structural biology, 75(7), 670-681.
DOI Scopus3 WoS3 Europe PMC3
2019 Lee, K. J., Tieu, W., Blanco-Rodriguez, B., Paparella, A. S., Yu, J., Hayes, A., . . . Abell, A. D. (2019). Sulfonamide-based inhibitors of biotin protein ligase as new antibiotic leads. ACS Chemical Biology, 14(9), 1990-1997.
DOI Scopus9 WoS8 Europe PMC5
2019 Jia, Y., Burbidge, C. A., Sweetman, C., Schutz, E., Soole, K., Jenkins, C., . . . Ford, C. M. (2019). An aldo-keto reductase with 2-keto- L-gulonate reductase activity functions in L-tartaric acid biosynthesis from vitamin C in Vitis vinifera. The Journal of biological chemistry, 294(44), 15932-15946.
DOI Scopus25 WoS24 Europe PMC19
2019 Schumann, N. C., Bruning, J., Marshall, A. C., & Abell, A. D. (2019). The role of N-terminal heterocycles in hydrogen bonding to α-chymotrypsin. Bioorganic and Medicinal Chemistry Letters, 29(3), 396-399.
DOI Scopus3 WoS3 Europe PMC1
2019 Marshall, A. C., Kidd, S. E., Lamont-Friedrich, S. J., Arentz, G., Hoffmann, P., Coad, B. R., & Bruning, J. B. (2019). Structure, mechanism, and inhibition of aspergillus fumigatus thioredoxin reductase. Antimicrobial Agents and Chemotherapy, 63(3), 1-15.
DOI Scopus32 WoS30 Europe PMC25
2019 Elzahhar, P. A., Alaaeddine, R., Ibrahim, T. M., Nassra, R., Ismail, A., Chua, B. S. K., . . . Belal, A. S. F. (2019). Shooting three inflammatory targets with a single bullet: Novel multi-targeting anti-inflammatory glitazones. European Journal of Medicinal Chemistry, 167, 562-582.
DOI Scopus45 WoS43 Europe PMC24
2019 Child, S. A., Flint, K. L., Bruning, J. B., & Bell, S. G. (2019). The characterisation of two members of the cytochrome P450 CYP150 family: CYP150A5 and CYP150A6 from Mycobacterium marinum. Biochimica et Biophysica Acta - General Subjects, 1863(5), 925-934.
DOI Scopus4 WoS3 Europe PMC2
2019 Chang, M. R., Ciesla, A., Strutzenberg, T. S., Novick, S. J., He, Y., Garcia-Ordonez, R. D., . . . Griffin, P. R. (2019). Unique Polypharmacology Nuclear Receptor Modulator Blocks Inflammatory Signaling Pathways. ACS Chemical Biology, 14(5), 1051-1062.
DOI Scopus8 WoS8 Europe PMC7
2018 Marshall, A., Keiller, B., Pederick, J., Abell, A., & Bruning, J. (2018). Crystal structure of bovine alpha-chymotrypsin in space group P65. Crystals, 8(12), 460.
DOI Scopus3 WoS3
2018 Frkic, R. L., Chua, B. S., Shin, Y., Pascal, B. D., Novick, S. J., Kamenecka, T. M., . . . Bruning, J. B. (2018). Structural and dynamic elucidation of a non-acid PPARγ partial agonist: SR1988. Nuclear Receptor Research, 5, 101350-1-101350-12.
DOI Europe PMC6
2018 Child, S., Naumann, E., Bruning, J., & Bell, S. (2018). Structural and functional characterisation of the cytochrome P450 enzyme CYP268A2 from Mycobacterium marinum. The Biochemical Journal, 475(4), 705-722.
DOI Scopus14 WoS14 Europe PMC9
2018 Marshall, A., Bond, C., & Bruning, J. (2018). Structure of Aspergillus fumigatus Cytosolic thiolase: trapped tetrahedral reaction intermediates and activation by monovalent cations. ACS Catalysis, 8(3), 1973-1989.
DOI Scopus5 WoS5
2018 Coleman, T., Wong, S., Podgorski, M., Bruning, J., De Voss, J., & Bell, S. (2018). Cytochrome P450 CYP199A4 from Rhodopseudomonas palustris catalyses heteroatom dealkylations, sulfoxidation and amide and cyclic hemiacetal formation. ACS Catalysis, 8(7), 5915-5927.
DOI Scopus36 WoS36
2018 Wegener, K. L., McGrath, A. E., Dixon, N. E., Oakley, A. J., Scanlon, D. B., Abell, A. D., & Bruning, J. (2018). Rational design of a 310-helical PIP-box mimetic targeting PCNA - the human sliding clamp. Chemistry - A European Journal, 24(44), 11325-11331.
DOI Scopus17 WoS16 Europe PMC16
2018 Thompson, A., Wegener, K., Booker, G., Polyak, S., & Bruning, J. (2018). Precipitant-ligand exchange technique reveals the ADP binding mode in Mycobacterium tuberculosis dethiobiotin synthetase. Acta Crystallographica Section D: Structural Biology, 74(10), 965-972.
DOI Scopus4 WoS4 Europe PMC3
2018 Frkic, R. L., Marshall, A. C., Blayo, A. -L., Pukala, T. L., Kamenecka, T. M., Griffin, P. R., & Bruning, J. B. (2018). PPARγ in complex with an antagonist and inverse agonist: a tumble and trap mechanism of the activation helix. iScience, 5, 69-79.
DOI Scopus60 WoS56 Europe PMC49
2018 Zheng, J., Corzo, C., Chang, M., Shang, J., Lam, V., Brust, R., . . . Griffin, P. (2018). Chemical crosslinking mass spectrometry reveals the conformational landscape of the activation helix of PPARγ; a model for ligand-dependent antagonism. Structure, 26(11), 1431-1439.
DOI Scopus29 WoS29 Europe PMC26
2018 Thompson, A. P., Salaemae, W., Pederick, J. L., Abell, A. D., Booker, G. W., Bruning, J. B., . . . Polyak, S. W. (2018). Mycobacterium tuberculosis Dethiobiotin Synthetase Facilitates Nucleoside Triphosphate Promiscuity through Alternate Binding Modes. ACS Catalysis, 8(11), 10774-10783.
DOI Scopus7 WoS6
2018 Wegener, K. L., McGrath, A. E., Dixon, N. E., Oakley, A. J., Scanlon, D. B., Abell, A. D., & Bruning, J. B. (2018). Front Cover: Rational Design of a 3<sub>10</sub>‐Helical PIP‐Box Mimetic Targeting PCNA, the Human Sliding Clamp (Chem. Eur. J. 44/2018). Chemistry – A European Journal, 24(44), 11234.
DOI
2017 Stevens, T., Song, S., Bruning, J., Choo, A., & Baxter, S. (2017). Expressing a moth abcc2 gene in transgenic Drosophila causes susceptibility to Bt Cry1Ac without requiring a cadherin-like protein receptor. Insect Biochemistry and Molecular Biology, 80, 61-70.
DOI Scopus48 WoS45 Europe PMC37
2017 Marshall, A., Kroker, A., Murray, L., Gronthos, K., Rajapaksha, H., Wegener, K., & Bruning, J. (2017). Structure of the sliding clamp from the fungal pathogen Aspergillus fumigatus (AfumPCNA) and interactions with Human p21. FEBS Journal, 284(6), 985-1002.
DOI Scopus11 WoS11 Europe PMC10
2017 Sternicki, L., Wegener, K., Bruning, J., Booker, G., & Polyak, S. (2017). Mechanisms governing precise protein biotinylation. Trends in Biochemical Sciences, 42(5), 383-394.
DOI Scopus23 WoS21 Europe PMC13
2017 Rajapaksha, H., Bhatia, H., Wegener, K., Petrovsky, N., & Bruning, J. (2017). X-ray crystal structure of rivoglitazone bound to PPARγ and PPAR subtype selectivity of TZDs. Biochimica et Biophysica Acta - General Subjects, 1861(8), 1981-1991.
DOI Scopus20 WoS21 Europe PMC12
2017 Frkic, R., He, Y., Rodriguez, B., Chang, M., Kuruvilla, D., Ciesla, A., . . . Bruning, J. (2017). Structure-Activity Relationship of 2,4-Dichloro-N-(3,5-dichloro-4-(quinolin-3-yloxy)phenyl)benzenesulfonamide (INT131) Analogs for PPARγ-Targeted Antidiabetics. Journal of Medicinal Chemistry, 60(11), 4584-4593.
DOI Scopus26 WoS24 Europe PMC20
2017 Zheng, J., Chang, M., Stites, R., Wang, Y., Bruning, J., Pascal, B., . . . Griffin, P. (2017). HDX reveals the conformational dynamics of DNA sequence specific VDR co-activator interactions. Nature Communications, 8(1), 923-1-923-13.
DOI Scopus38 WoS35 Europe PMC36
2016 Van Marrewijk, L., Polyak, S., Hijnen, M., Kuruvilla, D., Chang, M., Shin, Y., . . . Bruning, J. (2016). SR2067 reveals a unique kinetic and structural signature for PPARγ partial agonism. ACS Chemical Biology, 11(1), 273-283.
DOI Scopus35 WoS33 Europe PMC25
2016 Zhang, X., Bruning, J., George, J., & Abell, A. (2016). A mechanistic study on the inhibition of α-chymotrypsin by a macrocyclic peptidomimetic aldehyde. Organic and Biomolecular Chemistry, 14(29), 6970-6978.
DOI Scopus13 WoS13 Europe PMC6
2016 Stechschulte, L., Czernik, P., Rotter, Z., Tausif, F., Corzo, C., Marciano, D., . . . Lecka-Czernik, B. (2016). PPARG Post-translational modifications regulate bone formation and bone resorption. EBioMedicine, 10, 174-184.
DOI Scopus74 WoS72 Europe PMC65
2015 Jia, Y., Wong, D., Sweetman, C., Bruning, J., & Ford, C. (2015). New insights into the evolutionary history of plant sorbitol dehydrogenase. BMC Plant Biology, 15(1), 101-1-101-23.
DOI Scopus35 WoS32 Europe PMC22
2015 Kroker, A., & Bruning, J. (2015). p21 exploits residue Tyr151 as a tether for high-affinity PCNA binding. Biochemistry, 54(22), 3483-3493.
DOI Scopus27 WoS26 Europe PMC24
2015 Coleman, T., Chao, R., Bruning, J., De Voss, J., & Bell, S. (2015). CYP199A4 catalyses the efficient demethylation and demethenylation of para-substituted benzoic acid derivatives. RSC Advances, 5(64), 52007-52018.
DOI Scopus35 WoS34
2015 Marciano, D., Kuruvilla, D., Boregowda, S., Asteian, A., Hughes, T., Garcia-Ordonez, R., . . . Griffin, P. (2015). Pharmacological repression of PPARγ promotes osteogenesis. Nature Communications, 6(1), 7443-1-7443-7.
DOI Scopus110 WoS107 Europe PMC97
2015 Kojetin, D., Matta-Camacho, E., Hughes, T., Srinivasan, S., Nwachukwu, J., Cavett, V., . . . Nettles, K. (2015). Structural mechanism for signal transduction in RXR nuclear receptor heterodimers. Nature Communications, 6(1), 8013-1-8013-14.
DOI Scopus110 WoS109 Europe PMC94
2015 Kroker, A., & Bruning, J. (2015). Review of the structural and dynamic mechanisms of PPAR γ partial agonism. PPAR Research, 2015, 816856-1-816856-15.
DOI Scopus185 WoS169 Europe PMC144
2014 Chua, K., Pietsch, M., Zhang, X., Hautmann, S., Chan, H., Bruning, J., . . . Abell, A. (2014). Macrocyclic protease inhibitors with reduced peptide character. Angewandte Chemie International Edition, 53(30), 7828-7831.
DOI Scopus24 WoS23 Europe PMC15
2014 Sullivan, A., Raimondo, A., Schwab, T., Bruning, J., Froguel, P., Farooqi, I., . . . Whitelaw, M. (2014). Characterization of human variants in obesity-related SIM1 protein identifies a hot-spot for dimerization with the partner protein ARNT2. Biochemical Journal, 461(3), 403-412.
DOI Scopus9 WoS8 Europe PMC10
2014 Bersten, D., Bruning, J., Peet, D., & Whitelaw, M. (2014). Human variants in the Neuronal Basic Helix-Loop-Helix/ Per-Arnt-Sim (bHLH/PAS) transcription factor complex NPAS4/ARNT2 disrupt function. PLoS One, 9(1), e85768-1-e85768-11.
DOI Scopus22 WoS21 Europe PMC21
2014 Reddy, M., Breda, A., Bruning, J., Sherekar, M., Valluru, S., Thurman, C., . . . Sacchettini, J. (2014). Structure, activity, and inhibition of the carboxyltransferase β-Subunit of acetyl coenzyme a carboxylase (AccD6) from mycobacterium tuberculosis. Antimicrobial Agents and Chemotherapy, 58(10), 6122-6132.
DOI Scopus23 WoS20 Europe PMC16
2013 Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2013). Redefining the role of the quaternary shift in bacillus stearothermophilus phosphofructokinase. Biochemistry, 52(32), 5421-5429.
DOI Scopus9 WoS9 Europe PMC11
2013 Bonnefond, A., Raimondo, A., Stutzmann, F., Ghoussaini, M., Ramachandrappa, S., Bersten, D., . . . Froguel, P. (2013). Loss-of-function mutations in SIM1 contribute to obesity and Prader-Willi-like features. Journal of Clinical Investigation, 123(7), 3037-3041.
DOI Scopus118 WoS105 Europe PMC98
2013 Ramachandrappa, S., Raimondo, A., Cali, A., Keogh, J., Henning, E., Saeed, S., . . . Farooqi, I. (2013). Rare variants in single-minded 1 (SIM1) are associated with severe obesity. Journal of Clinical Investigation, 123(7), 3042-3050.
DOI Scopus140 WoS115 Europe PMC112
2012 Mosser, R., Reddy, M., Bruning, J., Sacchettini, J., & Reinhart, G. (2012). Structure of the apo form of Bacillus Stearothermophilus Phosphofructokinase. Biochemistry, 51(3), 769-775.
DOI Scopus9 WoS9 Europe PMC8
2011 Bruning, J., Murillo, A., Chacon, O., Barletta, R., & Sacchettini, J. (2011). Structure of the Mycobacterium tuberculosis D-alanine:D-alanine Ligase, a target of the antituberculosis drug D-Cycloserine. Antimicrobial Agents and Chemotherapy, 55(1), 291-301.
DOI Scopus131 WoS118 Europe PMC96
2011 Zhang, J., Chalmers, M., Stayrook, K., Burris, L., Wang, Y., Busby, S., . . . Griffin, P. (2011). DNA binding alters coactivator interaction surfaces of the intact VDR-RXR complex. Nature Structural and Molecular Biology, 18(5), 556-563.
DOI Scopus175 WoS160 Europe PMC155
2011 Chim, N., Habel, J., Johnston, J., Krieger, I., Miallau, L., Sankaranarayanan, R., . . . Goulding, C. (2011). The TB structural genomics consortium: a decade of progress. Tuberculosis, 91(2), 155-172.
DOI Scopus39 WoS33 Europe PMC32
2011 Choi, J., Banks, A., Kamenecka, T., Busby, S., Chalmers, M., Kumar, N., . . . Griffin, P. (2011). Anti-diabetic actions of a non-agonist PPARgamma ligand that blocks Cdk5-mediated phosphorylation. Nature, 477(7365), 477-481.
DOI Scopus480 WoS445 Europe PMC414
2010 Bruning, J., Parent, A., Gil, G., Zhao, M., Nowak, J., Pace, M., . . . Nettles, K. (2010). Coupling of receptor conformation and ligand orientation determine graded activity. Nature Chemical Biology, 6(11), 837-843.
DOI Scopus125 WoS121 Europe PMC97
2009 Reddy, M., Palaninathan, S., Bruning, J., Thurman, C., Smith, D., & Sacchettini, J. (2009). Structural insights into the mechanism of the allosteric transitions of Mycobacterium Tuberculosis cAMP receptor protein. Journal of Biological Chemistry, 284(52), 36581-36591.
DOI Scopus37 WoS32 Europe PMC30
2008 Nettles, K., Bruning, J., Gil, G., Nowak, J., Sharma, S., Hahm, J., . . . Greene, G. (2008). NFκB selectivity of estrogen receptor ligands revealed by comparative crystallographic analyses. Nature Chemical Biology, 4(4), 241-247.
DOI Scopus140 WoS139 Europe PMC120
2008 Dai, S., Chalmers, M., Bruning, J., Bramlett, K., Osborne, H., Montrose-Rafizadeh, C., . . . Griffin, P. (2008). Prediction of the tissue-specificity of selective estrogen receptor modulators by using a single biochemical method. Proceedings of the National Academy of Sciences of the United States of America, 105(20), 7171-7176.
DOI Scopus83 WoS71 Europe PMC66
2007 Nettles, K., Bruning, J., Gil, G., O'Neill, E., Nowak, J., Hughs, A., . . . Greene, G. (2007). Structural plasticity in the oestrogen receptor ligand-binding domain. EMBO Reports, 8(6), 563-568.
DOI Scopus121 WoS118 Europe PMC103
2007 Zhou, H., Nettles, K., Bruning, J., Kim, Y., Joachimiak, A., Sharma, S., . . . Katzenellenbogen, J. (2007). Elemental isomerism: a boron-nitrogen surrogate for a carbon-carbon double bond increases the chemical diversity of estrogen receptor ligands. Chemistry & Biology, 14(6), 659-669.
DOI Scopus63 WoS63 Europe PMC45
2007 Bruning, J., Chalmers, M., Prasad, S., Busby, S., Kamenecka, T., He, Y., . . . Griffin, P. (2007). Partial agonists activate PPARγ using a helix 12 independent mechanism. Structure, 15(10), 1258-1271.
DOI Scopus340 WoS327 Europe PMC291
2004 Bruning, J. B., & Shamoo, Y. (2004). Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-δ p66 subunit and flap endonuclease-1. Structure, 12(12), 2209-2219.
DOI Scopus184 WoS182 Europe PMC177
- Coleman, T., Podgorski, M. N., Doyle, M. L., Scaffidi-Muta, J. M., Campbell, E. C., Bruning, J. B., . . . Bell, S. (2023). Cytochrome P450-Catalyzed Oxidation of Halogen-Containing Substrates. SSRN Electronic Journal.
DOI

Year Citation
2021 Chua, B. S. K., & Bruning, J. B. (2021). PPARα and δ Ligand Design: Honing the Traditional Empirical Method with a More Holistic Overview. In M. Z. Badr (Ed.), Nuclear Receptors: The Art and Science of Modulator Design and Discovery (pp. 111-178). Cham, Switzerland: Springer.
DOI
2019 Frkic, R., & Bruning, J. (2019). Obtaining crystals of PPARγ ligand binding domain bound to small molecules. In Unknown Book (Vol. 1966, pp. 253-260).
DOI Scopus3 Europe PMC2

Year Citation
2023 Nguyen, S., Jovcevski, B., Truong, J. Q., Pukala, T. L., & Bruning, J. B. (2023). A structural model of the human plasminogen and <i>Aspergillus fumigatus</i> enolase complex: insights for antifungal drug discovery. In ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES Vol. 79 (pp. C706). INT UNION CRYSTALLOGRAPHY.
2010 Mosser, R., Reddy, M., Bruning, J., Sacchettini, J. C., & Reinhart, G. D. (2010). Redefining the Role of the Quaternary Shift in the Allosteric Inhibition of Bacillus Stearothermophilus Phosphofructokinase. In BIOPHYSICAL JOURNAL Vol. 98 (pp. 39A). CELL PRESS.
DOI
2007 Chalmers, M. J., Busby, S. A., Prasad, S., Bruning, J. B., Pascal, B. D., Southern, M. R., & Griffin, P. R. (2007). P97-S A High-Throughput Method for the characterization of Nuclear Receptor Dynamics. In J Biomol Tech. Vol. 18 (pp. 33). Tampa Florida.

Year Citation
2022 Toomes, C., McClure, B., Bruning, J., Page, E., Breen, J., Rehn, J., . . . White, D. (2022). Acquired JAK2 kinase domain mutations confer resistance to JAK inhibitors in in vitro models of a high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, New Directions in Leukaemia Research (NDLR).
2021 Toomes, C., McClure, B., Bruning, J., Breen, J., Rehn, J., Yeung, D., & White, D. (2021). Acquired JAK2 kinase domain mutations confer resistance to JAK inhibitors in cell models of a high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Oral, South Australian Health and Medical Research Institute (SAHMRI) Research Showcase.
2021 Toomes, C., McClure, B., Bruning, J., Breen, J., Rehn, J., Hughes, T., . . . White, D. (2021). Acquired mutations within the JAK2 kinase domain confer resistance to JAK inhibitors in in vitro models of a high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, Lorne Cancer Conference.
2021 Toomes, C., McClure, B., Bruning, J., Breen, J., Rehn, J., Hughes, T., . . . Yeung, D. (2021). Acquired mutations within the JAK2 kinase domain confer resistance to JAK inhibitors in in vitro models of high-risk ALL. Poster session presented at the meeting of Poster, European School of Haematology (ESH) Translational Research Conference.
2021 Toomes, C., McClure, B., Bruning, J., Breen, J., Rehn, J., Yeung, D., & White, D. (2021). Acquired mutations within the JAK2 kinase domain confer resistance to JAK inhibitors in in vitro models of a high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, Australian and New Zealand Children's Haematology/Oncology Group (ANZCHOG) Annual Scientific Meeting.
2021 Toomes, C., McClure, B., Bruning, J., Breen, J., Rehn, J., Yeung, D., & White, D. (2021). Acquired mutations within the JAK2 kinase domain confer resistance to JAK inhibitors in in vitro models of acute lymphoblastic leukaemia. Poster session presented at the meeting of Oral, Haematology Society of Australia & New Zealand (HSANZ) Blood Meeting.
2020 Toomes, C., McClure, B., Bruning, J., & White, D. (2020). The race is on in high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Oral, University of Adelaide School of Biological Sciences Postgraduate Symposium.
2020 Tavakoli, P., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent Activation of JAK/STAT Signaling Plays an Important Role in<i>in Vitro</i>Jaki Resistance in <i>TYK2</i>-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: ELSEVIER.
DOI
2020 Tavakoli, P. S., Eadie, L. N., Heatley, S. L., Bruning, J. B., & White, D. L. (2020). Persistent activation of JAK/STAT signaling plays an important role in in vitro Jaki resistance in TYK2-rearranged B-Cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of 62nd Annual Meeting of the American Society of Hematology. San Diego, California (virtual conference).
2020 Downes, C. E. J., McClure, B. J., Rehn, J., Breen, J., Bruning, J. B., Yeung, D. T., & White, D. L. (2020). Acquired Mutations within the JAK2 Kinase Domain Confer Resistance to JAK Inhibitors in an <i>in Vitro</i> model of a High-Risk Acute Lymphoblastic Leukemia. Poster session presented at the meeting of BLOOD. ELECTR NETWORK: ELSEVIER.
DOI WoS2
2019 Toomes, C., McClure, B., Mayne, B., Bruning, J., Heatley, S., Kok, C., . . . White, D. (2019). Identification and characterisation of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, Australian Society for Medical Research (ASMR) SA Meeting.
2019 Toomes, C., McClure, B., Bruning, J., & White, D. (2019). JAK2 kinase domain mutations confer resistance to JAK inhibitors in a pro-B cell model of high-risk acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, University of Adelaide School of Biological Sciences Postgraduate Symposium.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, Adelaide Protein Group (APG) Student Awards.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of ruxolitinib resistance mutations in Pro-B cells driven by a high-risk JAK2 fusion in B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of Oral, Australian Society for Medical Research (ASMR) SA Meeting.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and Characterisation of Ruxolitinib Resistant Mutations in JAK2-rearranged B-cell Acute Lymphoblastic Leukaemia. Poster session presented at the meeting of Poster, Australasian Genomic Technologies Association (AGTA) Annual Conference.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification of ruxolitinib resistance mutations in Pro-B cells driven by a high-risk B-ALL JAK2-fusion. Poster session presented at the meeting of Poster, Australian and New Zealand Children's Haematology/Oncology Group (ANZCHOG) Annual Scientific Meeting.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, South Australian Health and Medical Research Institute (SAHMRI) Annual Scientific Meeting.
2018 Downes, C. E., McClure, B. J., Mayne, B. T., Bruning, J. B., Heatley, S. L., Kok, C., . . . White, D. L. (2018). Identification and computational modelling of ruxolitinib resistant mutations in JAK2-rearranged B-cell acute lymphoblastic leukaemia. Poster session presented at the meeting of Poster, European Molecular Biology Laboratory (EMBL) Australia Postgraduate Symposium.

Year Citation
2018 Authors: Wegener K, Mcgrath A, Dixon N, Oakley A, Scanlon D, Abell A, Bruning J. Title: Rational Design of a 310-Helical PIP-Box Mimetic Targeting PCNA, the Human Sliding Clamp [Cover Profile]. Extent: N/A.
DOI

Year Citation
2025 Eadie, L. N., McDougal, D. P., Lagonik, E., Schutz, C. E., Conlin, T. S., Page, E. C., . . . White, D. L. (2025). The 5’ fusion partner modulates response to the STAMP inhibitor asciminib in <i>ABL</i> -rearranged ALL.
DOI
2025 McDougal, D., Pederick, J., Shearwin-Whyatt, L., Grützner, F., & Bruning, J. (2025). Structural evolution of the estrogen receptor regulatory domain.
DOI Europe PMC1
2025 McDougal, D., Pederick, J., Novick, S., Jovcevski, B., Warrender, A., Pascal, B., . . . Bruning, J. (2025). A ternary switch determines ERα LBD conformation.
DOI
2023 Lee, J., Bruning, J., & Bell, S. (2023). In Crystallo Reactions with an Engineered Cytochrome P450 Peroxygenase.
DOI
  • ARC DP (2024). DP240101500. A/Prof Stephen Bell ; Prof James De Voss ; A/Prof John Bruning. New biocatalysts for selective chemical oxidations under extreme conditions ($781,608).
  • Oracle Research Grant (2023). A/P John Bruning. High throughput protein folding for in-silico characterisation of designed and natural protein.
  • ARC DP (2023) DP230100609. A/Prof John Bruning ; Prof Frank Gruetzner ; Prof Patrick Griffin. Structural and molecular studies of endocrine disruption in Australia fauna ($563,171).
  • NHMRC Idea Grant (2021). Dr Nichoklas Eyre; Dr Amanda Aloia, Dr Thomas Avery; Dr John Bruning, Dr Tim Chataway. Development and mechanistic analysis of novel inhibitors of the dengue virus NS1 protein ($783,440)
  • ARC ITTC (2019). IC190100017. Prof Peter Dowd ; Prof William Skinner ; Prof Frank Neumann ; Prof Stephen Grano ; A/Prof Damith Ranasinghe ; Dr Tien-Fu Lu ; Prof Anton Kepic ; A/Prof Chaoshui Xu ; Prof Nigel Cook ; A/Prof Jixue Liu ; Prof David Lancaster .... Dr John Bruning. ARC Training Centre for Integrated Operations for Complex Resources ($3,703,664)
  • ARC LIEF Grant (2014). LE15010023.  Michael, Dr. Micahel Z; Booker, A/Prof., Grant W; Venter, Dr. Henrietta; Pitson, Prof. Stuart M; McKinnon, Prof. Ross A; Abell, Prof. Andrew D; Soole, A/Prof. Kathleen L; Sykes, Prof. Pamela J; Bruning, Dr. John B. “High Throughput Screening Facility.” ($860,000).
  • Environment Institute Major Collaborative Project (2015). Dr. Frank Reith, Prof. Peter Hoffmann, Dr. Florian Weiland, Dr. Benjamin Wade, Dr. John Bruning. ($50,000).
  • Biochemistry Discipline Collaborative Research Grant (2015-2018).  Bruning, JB and Booker, GW.  “Structural studies of Aspergillus fumigatus enzyme targets for drug discovery.” ($45,000).
  • AIB Labs, Research Infrastructure Fund (2015).  Parkinson-Lawrence, E., Polyak, S.W., Brooks, D., Booker, G.W., Forbes, B.E., Pitson, S., Cowin, A., Bruning, J., Butler, L. ($12,793).
  • IPAS Pilot Projects (2015).  Krogsgaard-Larsen, N.; Bruning, J.B.; Luiten, A.; Callen, D.; Zhang, X.Z.; Alaknanda, A.  “First Steps toward a Cancer Biosensor.” ($6,000).
  • Australian Synchrotron CAP (2013). CAP6919. Hrmova, M. and Bruning, J.B.  “X-ray crystal structures of plant and mammalian proteins, and protein design.”
  • Australian Synchrotron CAP (2014). CAP8221. Hrmova, M. and Bruning, J.B.  “X-ray crystal structures of plant and mammalian proteins, and protein design.”
  • BIOCHEM3000 – Molecular and Structural Biology
  • BIOCHEM3230 – Molecular and Structural Biology (Biomedical Science)
  • BIOCHEM3125 – Advanced Molecular Biology IIIA (Biochemistry)
  • BIOCHEM3001 – Cancer, Stem Cells and Development III
  • BIOCHEM3235 – Cancer, Stem Cells and Development III (Biomedical Science)
  • BIOCHEM3520 – Cancer, Stem Cells and Development (Theory) III
  • BIOCHEM3225 – Advanced Molecular Biology IIIB (Biochemistry)
  • BIOCHEM2500 – Biochemistry II: Molecular & Cell Biology
  • BIOCHEM2502 – Biochemistry II (Biotech): Molecular & Cell Biology
  • BIOCHEM2504 – Biochemistry II (Mol Biol):  Molecular & Cell Biology
  • BIOLOGY1201 – Biology I – Human Perspectives
  • CHEMENG 2015 - Principles of Biotechnology II
  • BIOTECH7000 – Advanced Research Platforms
  • BIOTECH7001 – Drug Discovery and Development
  • Science2300 – Principles and Practice of Research

 

Date Role Research Topic Program Degree Type Student Load Student Name
2025 Co-Supervisor Exploring the molecular interplay of highly related tumour suppressor proteins that bind identical DNA motifs Master of Philosophy (Medical Science) Master Full Time Miss Katarina Anastasia Amerl
2025 Co-Supervisor Characterising Endocrine Disrupting Chemicals and Synthetic Agonists as Peroxisome Proliferator Activated Receptor Ligands. Doctor of Philosophy Doctorate Full Time Mr Samuel Luke Wallis
2025 Co-Supervisor Investigating the role of TLR4 antagonists and the effects on chronic pain: a structural biology journey Doctor of Philosophy under a Jointly-awarded Degree Agreement with Doctorate Full Time Mr Anston Savio D'silva
2025 Co-Supervisor Exploring the molecular interplay of highly related tumour suppressor proteins that bind identical DNA motifs - Master Full Time Miss Katarina Anastasia Amerl
2025 Co-Supervisor Characterising Endocrine Disrupting Chemicals and Synthetic Agonists as Peroxisome Proliferator Activated Receptor Ligands. Doctor of Philosophy Doctorate Full Time Mr Samuel Luke Wallis
2025 Co-Supervisor Investigating the role of TLR4 antagonists and the effects on chronic pain: a structural biology journey Doctor of Philosophy Doctorate Full Time Mr Anston Savio D'silva
2024 Co-Supervisor Characterization of peroxygenase activity in CYP154 enzymes from the thermophilic bacterium Thermobifida fusca Doctor of Philosophy Doctorate Full Time Ms Oghenesivwe Osiebe
2024 Principal Supervisor The design of novel peptide mimetics or small molecule inhibitors of proliferating cell nuclear antigen (PCNA) for the development of broad-spectrum cancer therapeutics. Doctor of Philosophy Doctorate Full Time Miss Dayna Louise Holroyd
2024 Co-Supervisor Innate phagocytosis from a basic feeding behaviour to a regulated defence action Doctor of Philosophy Doctorate Full Time Mr Wei Da Ho
2024 Principal Supervisor Selective Androgen Receptor Modulators (SARMs) for the Treatment of Androgen Receptor positive (AR+) and Estrogen Receptor Positive (ER+) Breast Cancer. Doctor of Philosophy Doctorate Full Time Mr Manjit Rana
2024 Co-Supervisor Exploration and Exploitation of Novel Thermally Adapted Cytochromes P450 and their Biotechnological Uses Doctor of Philosophy Doctorate Full Time Mr Delroy Sheng Yu Huang
2024 Co-Supervisor The Study of Cholesterol Oxidizing Enzymes from Actinobacteria Doctor of Philosophy Doctorate Full Time Dr Gashaw Getaneh Dagnaw
2024 Principal Supervisor The design of novel peptide mimetics or small molecule inhibitors of proliferating cell nuclear antigen (PCNA) for the development of broad-spectrum cancer therapeutics. Doctor of Philosophy Doctorate Full Time Miss Dayna Louise Holroyd
2024 Co-Supervisor Characterization of peroxygenase activity in CYP154 enzymes from the thermophilic bacterium Thermobifida fusca Doctor of Philosophy Doctorate Full Time Ms Oghenesivwe Osiebe
2024 Co-Supervisor Innate phagocytosis from a basic feeding behaviour to a regulated defence action Doctor of Philosophy Doctorate Full Time Mr Wei Da Ho
2024 Co-Supervisor Exploration and Exploitation of Novel Thermally Adapted Cytochromes P450 and their Biotechnological Uses Doctor of Philosophy Doctorate Full Time Mr Delroy Sheng Yu Huang
2024 Co-Supervisor The Study of Cholesterol Oxidizing Enzymes from Actinobacteria Doctor of Philosophy Doctorate Full Time Dr Gashaw Getaneh Dagnaw
2024 Principal Supervisor Selective Androgen Receptor Modulators (SARMs) for the Treatment of Androgen Receptor positive (AR+) and Estrogen Receptor Positive (ER+) Breast Cancer. Doctor of Philosophy Doctorate Full Time Mr Manjit Rana
2023 Co-Supervisor Investigation of transcriptional regulators as a novel approach to treat ovarian cancer Doctor of Philosophy Doctorate Full Time Ms Suet Yee Too
2023 Co-Supervisor Investigation of transcriptional regulators as a novel approach to treat ovarian cancer Doctor of Philosophy Doctorate Full Time Ms Suet Yee Too
2022 Co-Supervisor Researching the Interaction, Structure and Function of S-Proteins in Almond Doctor of Philosophy under a Jointly-awarded Degree Agreement with Doctorate Full Time Ms Isis Pinner
2022 Co-Supervisor Researching the Interaction, Structure and Function of S-Proteins in Almond Doctor of Philosophy Doctorate Full Time Ms Isis Pinner

Date Role Research Topic Program Degree Type Student Load Student Name
2021 - 2025 Principal Supervisor Towards the Development of a Protein Based Gold Biosensor Doctor of Philosophy Doctorate Full Time Mr Akhil Kumar
2020 - 2024 Principal Supervisor Insights into Peptidomimetic Approaches for Inhibiting the DNA Sliding Clamps Doctor of Philosophy Doctorate Full Time Miss Bethiney Chantel Vandborg
2020 - 2023 Co-Supervisor Development of 2-Aminoquinoline Derivatives with Improved Drug-Like Character as Small Molecule Inhibitors for a Protein-Protein Interaction Domain Doctor of Philosophy Doctorate Part Time Ms Ellen Lillian Swan
2019 - 2021 Co-Supervisor Targeting PCNA with Cell and Nuclear Permeable p21- derived Peptides Master of Philosophy Master Full Time Miss Theresa Chav
2019 - 2023 Principal Supervisor The structural selectivity and various ligands of PPAR gamma Doctor of Philosophy Doctorate Full Time Mr Benjamin Soon Kai Chua
2019 - 2025 Principal Supervisor A Structural Analysis of Estrogen Receptor Evolution and Allosteric Regulation Doctor of Philosophy Doctorate Full Time Mr Daniel Patrick McDougal
2018 - 2021 Principal Supervisor Structural and Biochemical Insights into Antifungal Drug Targets from Aspergillus fumigatus Doctor of Philosophy Doctorate Full Time Ms Stephanie Nguyen
2018 - 2023 Principal Supervisor Functional and Structural Characterisation of Bacterial ATP-grasp Ligases Doctor of Philosophy Doctorate Full Time Mr Jordan Luke Pederick
2018 - 2022 Co-Supervisor Precision Medicine Approaches for JAK2-Rearranged Acute Lymphoblastic Leukaemia: The Efficacy of Targeted Therapies and Molecular Mechanisms of Drug Resistance Doctor of Philosophy Doctorate Full Time Miss Charlotte Emma-Jeane Toomes
2018 - 2021 Co-Supervisor Approaches to study protein interactions Doctor of Philosophy Doctorate Full Time Miss Aimee Jade Horsfall
2018 - 2019 Co-Supervisor Structural and Biophysical Characterisation of Mycobacterium tuberculosis Dethiobiotin Synthetase Doctor of Philosophy Doctorate Full Time Andrew Philip Thompson
2018 - 2023 Co-Supervisor Structural and Functional Investigations of Cytochrome P450 Enzymes from Mycobacterium Species Doctor of Philosophy Doctorate Full Time Miss Hebatalla Ahmed Ibrahim Mohamed
2017 - 2021 Principal Supervisor Understanding PPARg-Ligand Interactions to Treat Type 2 Diabetes Doctor of Philosophy Doctorate Full Time Miss Rebecca Louise Frkic
2017 - 2019 Co-Supervisor Microbial Biosynthesis of Grapevine Specialised Metabolites Master of Philosophy Master Full Time Miss Emi Kaye Schutz
2016 - 2017 Principal Supervisor Defining the Key Residues in PCNA-Protein Interactions Master of Philosophy Master Part Time Alice Jane Kroker
2016 - 2021 Co-Supervisor Eukaryotic elongation factor 2 kinase (eEF2K): its relation to cancer cell survival, autophagy and other cell signalling pathways Doctor of Philosophy Doctorate Full Time Mr Roman Valeryevich Lenchine
2015 - 2021 Co-Supervisor Structural Characterization of Lysogeny-Promoting Transcription Factor of Bacteriophage 186 Doctor of Philosophy Doctorate Full Time Mr Jia Quyen Truong
2014 - 2018 Principal Supervisor Structural and Biochemical Studies on Three Aspergillus fumigatus Proteins That Present as Targets for Novel Antifungal Drugs Doctor of Philosophy Doctorate Full Time Andrew Craig Marshall
2014 - 2016 Co-Supervisor Molecular and Structural Characterization of the Candidate Enzymes Responsible for Tartaric Acid Synthesis in the Grapevine Doctor of Philosophy Doctorate Full Time Mr Yong Jia

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