Sam Henderson

School of Agriculture, Food and Wine

Faculty of Sciences, Engineering and Technology

Sam is a researcher with expertise in plant science, cell membrane biology, and ion channels. His first postdoctoral position was undertaken at the ARC Centre of Excellence in Plant Energy Biology, investigating salinity tolerance in crop plants. He carried out further postdoctoral work at CSIRO, investigating the molecular basis of apomixis in plants. In 2019, he joined Andrea Yool’s team in the Faculty of Health and Medical Sciences, to study the fundamental roles of human aquaporins.

Aquaporins (AQPs) are channels that conduct water and small solutes across cell membranes. Our laboratory aims to understand the physiological role of water and ion conductivity of human AQPs in health and disease.

We use a range of techniques including:

• Recombinant protein production and purification
• Patch-clamp electrophysiology
• Two-electrode voltage clamp (TEVC) electrophysiology
• Cell swelling assays
• Cell culture
• Microbiology
• Advanced microscopy

Career
Publications
Grants and Funding
Supervision
Professional Activities
Contact

Appointments

Date	Position	Institution name
2019 - ongoing	Postdoctoral Research Fellow	University of Adelaide
2018 - 2019	Postdoctoral Scientist	Commonwealth Scientific and Industrial Research Organisation
2014 - 2017	Postdoctoral Research Associate	University of Adelaide
2007 - 2010	Research Officer	University of Adelaide
2006 - 2007	Technical Officer	Australian Centre for Plant Functional Genomics, University of Adelaide

Education

Date Institution name Country Title
2014 University of Adelaide Australia PhD
2005 Flinders University Australia Bachelor of Biotechnology (Hons)
Research Interests

Biochemistry & Molecular Biology Plant Cell and Molecular Biology Receptors and Membrane Biology

Date	Institution name	Country	Title
2014	University of Adelaide	Australia	PhD
2005	Flinders University	Australia	Bachelor of Biotechnology (Hons)

Journals

Year	Citation
2023	Henderson, S. W., Nakayama, Y., Whitelaw, M. L., Bruning, J. B., Anderson, P. A., Tyerman, S. D., . . . Yool, A. J. (2023). Proteoliposomes reconstituted with human aquaporin-1 reveal novel single ion channel properties. Biophysical Reports, 3(1), 100100-1-100100-15. DOI Scopus2 WoS2 Europe PMC1
2022	Wu, Y., Henderson, S. W., Walker, R. R., & Gilliham, M. (2022). Root-Specific Expression of Vitis vinifera VviNPF2.2 Modulates Shoot Anion Concentration in Transgenic Arabidopsis. Frontiers in Plant Science, 13, 1-13. DOI Scopus1 WoS1 Europe PMC1
2022	Qiu, J., Henderson, S. W., Tester, M., Roy, S. J., & Gilliham, M. (2022). SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl- accumulation and salt tolerance in Arabidopsis thaliana (vol 67, pg 4495, 2016). JOURNAL OF EXPERIMENTAL BOTANY, 73(18), 1 page. DOI
2022	Henderson, S. W., Nourmohammadi, S., Ramesh, S. A., & Yool, A. J. (2022). Aquaporin ion conductance properties defined by membrane environment, protein structure, and cell physiology. Biophysical Reviews, 14(1), 181-198. DOI Scopus9 WoS7 Europe PMC7
2021	Chow, P. H., Cox, C. D., Pei, J. V., Anabaraonye, N., Nourmohammadi, S., Henderson, S. W., . . . Yool, A. J. (2021). Inhibition of the Aquaporin-1 Cation Conductance by Selected Furan Compounds Reduces Red Blood Cell Sickling.. Frontiers in pharmacology, 12, 1-13. DOI Scopus3 WoS3 Europe PMC3
2021	Qu, Y., Guan, R., Bose, J., Henderson, S. W., Wege, S., Qiu, L., & Gilliham, M. (2021). Soybean CHX-type ion transport protein GmSALT3 confers leaf Na⁺ exclusion via a root derived mechanism, and Cl⁻ exclusion via a shoot derived process. Plant, Cell and Environment, 44(3), 856-869. DOI Scopus20 WoS13 Europe PMC15
2021	Zhou‐Tsang, A., Wu, Y., Henderson, S. W., Walker, A. R., Borneman, A. R., Walker, R. R., & Gilliham, M. (2021). Grapevine salt tolerance. Australian Journal of Grape and Wine Research, 27(2), 149-168. DOI Scopus26 WoS12
2020	Munns, R., Day, D. A., Fricke, W., Watt, M., Arsova, B., Barkla, B. J., . . . Tyerman, S. D. (2020). Energy costs of salt tolerance in crop plants. New Phytologist, 225(3), 1072-1090. DOI Scopus277 WoS229 Europe PMC96
2020	Wu, Y., Henderson, S. W., Wege, S., Zheng, F., Walker, A. R., Walker, R. R., & Gilliham, M. (2020). The grapevine NaE sodium exclusion locus encodes sodium transporters with diverse transport properties and localisation. Journal of Plant Physiology, 246-247, 153113-1-153113-11. DOI Scopus14 WoS7 Europe PMC4
2020	Henderson, S. W., Henderson, S. T., Goetz, M., & Koltunow, A. M. G. (2020). Efficient crispr/cas9-mediated knockout of an endogenous phytoene desaturase gene in t1 progeny of apomictic hieracium enables new strategies for apomixis gene identification. Genes, 11(9), 1-16. DOI Scopus5 WoS5 Europe PMC4
2019	Tyerman, S., Munns, R., Fricke, W., Arsova, B., Barkla, B., Bose, J., . . . Wen, Z. (2019). Energy costs of salinity tolerance in crop plants. New Phytologist, 221(1), 25-29. DOI Scopus53 WoS46 Europe PMC24
2018	Henderson, S., Dunlevy, J., Wu, Y., Blackmore, D., Walker, R., Edwards, E., . . . Walker, A. (2018). Functional differences in transport properties of natural HKT1;1 variants influence shoot Na⁺ exclusion in grapevine rootstocks. New Phytologist, 217(3), 1113-1127. DOI Scopus55 WoS43 Europe PMC19
2018	Henderson, S., Wege, S., & Gilliham, M. (2018). Plant cation-chloride cotransporters (CCC): evolutionary origins and functional insights. International Journal of Molecular Sciences, 19(2), 492-1-492-12. DOI Scopus19 WoS13 Europe PMC5
2018	Walker, R., Blackmore, D., Gong, H., Henderson, S., Gilliham, M., & Walker, A. (2018). Analysis of the salt exclusion phenotype in rooted leaves of grapevine (Vitis spp.). Australian Journal of Grape and Wine Research, 24(3), 317-326. DOI Scopus8 WoS9
2017	Wege, S., Gilliham, M., & Henderson, S. (2017). Chloride: not simply a 'cheap osmoticum', but a beneficial plant macronutrient. Journal of Experimental Botany, 68(12), 3057-3069. DOI Scopus91 WoS77 Europe PMC29
2017	Byrt, C., Zhao, M., Kourghi, M., Bose, J., Henderson, S., Qiu, J., . . . Tyerman, S. (2017). Non-selective cation channel activity of aquaporin AtPIP2;1 regulated by Ca²⁺ and pH. Plant, Cell and Environment, 40(6), 802-815. DOI Scopus145 WoS124 Europe PMC69
2016	Li, B., Byrt, C., Qiu, J., Baumann, U., Hrmova, M., Evrard, A., . . . Roy, S. (2016). Identification of a stelar-localized transport protein that facilitates root-to-shoot transfer of chloride in arabidopsis. Plant Physiology, 170(2), 1014-1029. DOI Scopus88 WoS72 Europe PMC46
2016	Qiu, J., Henderson, S., Tester, M., Roy, S., & Gilliham, M. (2016). SLAH1, a homologue of the slow type anion channel SLAC1, modulates shoot Cl⁻ accumulation and salt tolerance in Arabidopsis thaliana. Journal of Experimental Botany, 67(15), 4495-4505. DOI Scopus72 WoS60 Europe PMC28
2015	Henderson, S., Wege, S., Qiu, J., Blackmore, D., Walker, A., Tyerman, S., . . . Gilliham, M. (2015). Grapevine and Arabidopsis cation-chloride cotransporters localize to the Golgi and trans-Golgi network and indirectly influence long-distance ion transport and plant salt tolerance. Plant Physiology, 169(3), 2215-2229. DOI Scopus64 WoS54 Europe PMC34
2014	Henderson, S., Baumann, U., Blackmore, D., Walker, A., Walker, R., & Gilliham, M. (2014). Shoot chloride exclusion and salt tolerance in grapevine is associated with differential ion transporter expression in roots. BMC Plant Biology, 14(1), 273-1-273-18. DOI Scopus79 WoS74 Europe PMC33
2013	Conn, S., Hocking, B., Dayod, M., Xu, B., Athman, A., Henderson, S., . . . Gilliham, M. (2013). Protocol: optimising hydroponic growth systems for nutritional and physiological analysis of Arabidopsis thaliana and other plants. Plant Methods, 9(1), 4. DOI Scopus143 WoS136 Europe PMC88

Book Chapters

Year	Citation
2015	Henderson, S., & Gilliham, M. (2015). The "Gatekeeper" Concept: Cell-Type Specific Molecular Mechanisms of Plant Adaptation to Abiotic Stress. In R. Laitinen (Ed.), Molecular Mechanisms in Plant Adaptation (pp. 83-116). New Jersey: Wiley. DOI Scopus19

Conference Items

Year	Citation
2016	Byrt, C. S., Zhao, M., Kourghi, M., Bose, J., Henderson, S. W., Qui, J., . . . Tyerman, S. (2016). Charging up water transport with plant aquaporins permeable to ions. Poster session presented at the meeting of Combio2016. Brisbane, Australia.

Date	Grant Title	Investigators	Funding Body
2020 - 2021	Replacing frog oocytes and animal antibodies in aquaporin research: Developing an animal-free analysis platform to assess human Aquaporin-1 channels involved in cancer cell migration.	Henderson, SW; Yool AJ; Martinac B	The Medical Advances Without Animals (MAWA) Trust

Past Higher Degree by Research Supervision (University of Adelaide)

Date	Role	Research Topic	Program	Degree Type	Student Load	Student Name
2015 - 2019	Co-Supervisor	Functional Study of Grapevine (Vitis vinifera L.) Membrane Ion Transporters Related to Salt Tolerance	Doctor of Philosophy	Doctorate	Full Time	Miss Yue Wu

Memberships

Date Role Membership Country
2010 - ongoing - Australian Society for Plant Scientists (ASPS) Australia

Date	Role	Membership	Country
2010 - ongoing	-	Australian Society for Plant Scientists (ASPS)	Australia

Email: sam.henderson@adelaide.edu.au

Sam Henderson

Appointments

Education

Research Interests

Journals

Book Chapters

Conference Items

Past Higher Degree by Research Supervision (University of Adelaide)

Memberships

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