Nathan Rout-Pitt

Dr Nathan Rout-Pitt

Grant funded researcher (B)

Adelaide Medical School

Faculty of Health and Medical Sciences

Eligible to supervise Masters and PhD (as Co-Supervisor) - email supervisor to discuss availability.

I I completed my PhD at the University of Adelaide in 2015 with a focus on bone disease in the group of lysosomal storage disorders, Mucopolysaccharidoses.

In late 2015, I joined the CF Airway Research Group (CFARG) where I lead the vector production core and have produced lentiviral vectors for CFARG and Australia-wide laboratories. During this time I have developed and published a method to scale up vector production by as much as 5x. I also developed and published methods for bronchoscopic delivery of liquids such as lentiviral vectors, cells and drugs to single lobes of rat lungs.

In 2018 I began investigating airway remodelling and fibrosis development in CF lungs. A $75,000 Women's and Children's foundation grant allowed me to begin exploring the alterations in cellular architecture of our D508 and 510X CF rat models and their sensitivity to the process of epithelial-mesenchymal transition, which under chronic inflammatory conditions leads to fibrosis.

I briefly left CFARG for a year (2021) to return to lysosomal storage disorders and bone disease, this time looking at Gaucher disease before returning. Upon my return, I have continued with my previous investigations into the developmental/mechanistic pathways leading to lung fibrosis in CF. I have now begun a new project investigating CF bone disease (CFBD) which typically presents with decreased bone mineral volume and short stature and higher risk of fractures. Decreased trabecular number around the growth plate of long bones and previously reported issues at the hypertrophic zone, there is a need to better understand mechanisms at play in the growth plate that could be leading to CFBD.

Honours projects currently available

Project 1

Title: Identifying altered pathways that lead to increased lung stiffness in CF rats models

Description: Our group has access to two CF rat models containing different mutations (A Phe508del mutation which results in misfolded version of the protein, and a KO mutation with a truncated non-functional protein). CF rat models typically do not develop overt lung disease like humans, however, our newer data suggests that there is evidence of more subtle alterations to the lungs of our CF rat models including lung stiffness; a sign of fibrotic tissue. We have also shown that the gene expression profiles of these two CF rat models are not only different to wildtype rats, but also each other. One of the more obvious changes is collagen type 1 production which is increased in Phe508del rats, but decreased in KO rats.

There are currently a number of sub-projects within this project looking at:

1) Further characterising changes in signalling pathways in these rats using RNAseq,

2) Understanding the effect of Phe508del mutant CFTR protein on signalling pathways,

3) Developing and characterising a rat fibrotic lung model, and

4) Determining whether current CFTR modulator therapies or potential lentiviral gene therapies can fully correct these altered signalling pathways.    

These projects will utilise a range of skills including RNAseq analysis, qPCR, Western blotting, cell culture, and immunohistochemistry. These projects may also involve mass spectrometry for lipid analysis and the use of a novel In Vivo Oxidative Status (IVOS) probe to measure oxidative stress levels in the CF rats. 

Co-supervisor(s): A. Prof Martin Donnelley, Dr Alexandra McCarron, A. Prof Erik Noschka (Project dependent)

Projects available for: Honours and Masters

Location: Women's and Children's Hospital

Research project start: Semester 1 or 2

 

 

    2024 N. Rout-Pitt, A. McCarron, D. Parsons, M. Donnelley, Fall Path to a cure (PTAC) Pilot and Feasibility Award Program of the Cystic Fibrosis Foundation (CFF): 'Unravelling the Role of Epithelial-Mesenchymal Transition (EMT) in Cystic Fibrosis Lung Disease: Insights, Challenges, and Therapeutic Prospects', $175,000

    2019 N. Rout-Pitt, N. Farrow, J. Delhove, D. Parsons, M. Donnelley, WCH Foundation Grant: “Cell plasticity of the airways: Understanding the stem cell niche to optimise gene therapy and stem cell targeting”, $75,000

    2018 N. Rout-Pitt, Cure 4 Cystic Fibrosis Foundation Grant: "Lentiviral vector production facility management", $41,828

    2017 M. Donnelley, C. McIntyre, N. Rout-Pitt, A. McCarron, D. Parsons, Channel 7 Children's Research Foundation Grant: “Improving the efficiency of cystic fibrosis airway gene therapy”, $74,213

     

    I am passionate about science and have over ten years research experience as a molecular biologist in bone and respiratory diseases in multi-disciplinary teams delivering high quality research.

    I have supervised 3rd year placement, honours, masters and PhD students. My first student in the Masters of Biotechnology program within the School of Biological Sciences provided with a glowing thank you in her thesis.

    '...I would like to thank my other supervisor, Dr Nathan Rout-Pitt. Thank you for your careful guidance to my every experiment and giving me the most detailed opinions in the process of my writing. I think he is the most responsible and patient supervisor I have seen in Australia in the past two years. Even in my 18 years of school, I haven't met such a good supervisor as him'

    • Current Higher Degree by Research Supervision (University of Adelaide)
      Date Role Research Topic Program Degree Type Student Load Student Name
      2024 Co-Supervisor Improving the effectiveness of gene therapy for cystic fibrosis airway disease Doctor of Philosophy Doctorate Full Time Miss Farzaneh Rahvar
      2023 Co-Supervisor Understanding and improving gene vector targeting with lentiviral magnetic nanoparticles Doctor of Philosophy under a Jointly-awarded Degree Agreement with Doctorate Part Time Ms Madison Michelle Davis
    • Position: Grant funded researcher (B)
    • Phone: 81619179
    • Email: nathan.rout-pitt@adelaide.edu.au
    • Campus: Womens & Childrens Hospital
    • Building: WCH - Gilbert Building, floor 6
    • Org Unit: Women's and Children's Health
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